scholarly journals Effect of cat litters on feline coronavirus infection of cell culture and cats

2019 ◽  
Vol 22 (4) ◽  
pp. 350-357 ◽  
Author(s):  
Diane Addie ◽  
Lene Houe ◽  
Kirsty Maitland ◽  
Giuseppe Passantino ◽  
Nicola Decaro
Keyword(s):  
Cell Culture ◽  
Virus Infection ◽  
Real Life ◽  
In Vitro Study ◽  
Oral Route ◽  
Virus Shedding ◽  
Virus Load ◽  
Fuller’S Earth ◽  
Feline Coronavirus

Objectives Feline infectious peritonitis (FIP) is caused by infection with feline coronavirus (FCoV). FCoV is incredibly contagious and transmission is via the faecal–oral route. FCoV infection, and therefore FIP, is most common in breeder and rescue catteries, where many cats are kept indoors, using litter trays. Whether it is possible to break the cycle of FCoV infection and reinfection using cat litters has never been investigated. The aim of the study was to examine the effect of cat litters on FCoV infectivity and virus load in multi-cat households, and transmission frequency. Methods Fifteen cat litters were mixed and incubated with FCoV, centrifuged and the supernatants tested in vitro for the ability to prevent virus infection of cell culture. To test applicability of in vitro results to real life, virus load was measured in two households in a double crossover study of four Fuller’s earth-based cat litters by testing rectal swabs using FCoV reverse transcriptase quantitative PCR. Results Four litters abrogated FCoV infection of cell culture, nine reduced it to a greater or lesser extent and two had no effect. One brand had different virus inhibitory properties depending on where it was manufactured. Fuller’s earth-based litters performed best, presumably by adsorbing virus. In the field study, there appeared to be less virus shedding on one Fuller’s earth-based cat litter. Conclusions and relevance The in vitro study successfully identified cat litters that inactivate FCoV; such litters exist so do not need to be developed. Fuller’s earth-based litters best prevented infection of cell culture, but did not completely abrogate FCoV transmission in two multi-cat households. A dust-free clumping Fuller’s earth litter appeared to fare best, but virus shedding also varied on the control litters, complicating interpretation. Sawdust-based cat litters are not useful in FCoV-endemic households because they track badly and have a poor effect on virus infection.

scholarly journals Effects of Ethanol and Acetaldehyde on Tight Junction Integrity: In Vitro Study in a Three Dimensional Intestinal Epithelial Cell Culture Model

PLoS ONE ◽  
2012 ◽  
Vol 7 (4) ◽  
pp. e35008 ◽  
Author(s):  
Elhaseen Elamin ◽  
Daisy Jonkers ◽  
Kati Juuti-Uusitalo ◽  
Sven van IJzendoorn ◽  
Freddy Troost ◽  
...  
Keyword(s):  
Cell Culture ◽  
Epithelial Cell ◽  
Intestinal Epithelial Cell ◽  
Three Dimensional ◽  
In Vitro Study ◽  
Intestinal Epithelial ◽  
Cell Culture Model ◽  
Epithelial Cell Culture ◽  
Culture Model

EFFECT OF TRIGONA HONEY ON Escherichia Coli CELL CULTURE GROWTH: IN VITRO STUDY

2019 ◽  
Vol 5 (2) ◽  
pp. 10
Author(s):  
Mohammad Kafaween ◽  
Abu Hilmi ◽  
Rao Khan ◽  
Mabrouka BOUACHA ◽  
Malik Amonov
Keyword(s):  
Escherichia Coli ◽  
Cell Culture ◽  
In Vitro Study ◽  
Vitro Study ◽  
Coli Cell ◽  
Culture Growth ◽  
Cell Culture Growth

scholarly journals Evaluation of Antiviral Activities of Curcumin Derivatives against HSV-1 in Vero Cell Line

2010 ◽  
Vol 5 (12) ◽  
pp. 1934578X1000501 ◽  
Author(s):  
Keivan Zandi ◽  
Elissa Ramedani ◽  
Khosro Mohammadi ◽  
Saeed Tajbakhsh ◽  
Iman Deilami ◽  
...  
Keyword(s):  
Cell Culture ◽  
Antiviral Activity ◽  
Vero Cell ◽  
Cell Line ◽  
Antiviral Agents ◽  
Antiviral Drug ◽  
In Vitro Study ◽  
Vero Cell Line ◽  
Hsv 1

Antiviral drug resistance is one of the most common problems in medicine, and, therefore, finding new antiviral agents, especially from natural resources, seems to be necessary. This study was designed to assay the antiviral activity of curcumin and its new derivatives like gallium-curcumin and Cu-curcumin on replication of HSV-1 in cell culture. The research was performed as an in vitro study in which the antiviral activity of different concentrations of three substances including curcumin, Gallium-curcumin and Cu-curcumin were tested on HSV-1. The cytotoxicity of the tested compounds was also evaluated on the Vero cell line. The CC50 values for curcumin, gallium-curcumin and Cu-curcumin were 484.2 μg/mL, 255.8 μg/mL and 326.6 μg/mL, respectively, and the respective IC50 values 33.0 μg/mL, 13.9 μg/mL and 23.1 μg/mL. The calculated SI values were 14.6, 18.4 and 14.1, respectively. The results showed that curcumin and its new derivatives have remarkable antiviral effects on HSV-1 in cell culture.

Evaluation of Osteoblast Responses to Silver-Hydroxyapatite/Titania Nanoparticles In Vitro

2007 ◽  
Vol 330-332 ◽  
pp. 447-450 ◽  
Author(s):  
Jing Chao Zhang ◽  
Juan Liao ◽  
An Chun Mo ◽  
Hong Kun Wu ◽  
Yu Bao Li ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Proliferation ◽  
In Vitro Study ◽  
Vitro Study ◽  
Titania Nanoparticles ◽  
Respiration Rates ◽  
The Difference

In the present in vitro study, osteoblasts proliferation, vitality and ultrastructure were investigated when cultured in the presence of silver-hydroxyapatite/titania nanoparticles (nAg_HA/TiO2) compared to HA nanoparticles (nHA) at various concentrations and cell culture without nanoparticles for up to 120 hours. Results confirmed the detrimental influences of both nAg_HA/TiO2 and nHA on osteoblast growth.Cell vitality was slightly higher during the earlier 24h, but after that was inhibited. Both cell proliferation and vitality by addition of nanoparticles were restricted with concentrations of nanoparticles increasing. However, the respiration rates by addition of nanoparticles were showed higher than that of the cell culture without nanoparticles. No remarkable ultrastructure changes were showed in the osteoblasts exposed nanoparticles. The difference in cell proliferation, vitality and ultrastructure between nAg_HA/TiO2 and nHA were insignificant. It was demonstrated that biocompatibility of nAg_HA/TiO2 is almost the same as nHA.

Aerosol vehicle for Delivery of Epidermal Cells – An in Vitro Study

1997 ◽  
Vol 5 (3) ◽  
pp. 153-156 ◽  
Author(s):  
Andre Bahoric ◽  
A Robertson Harrop ◽  
Howard M Clarke ◽  
Ronald M Zuker
Keyword(s):  
Cell Culture ◽  
Cell Proliferation ◽  
In Vitro Study ◽  
Daily Basis ◽  
Epidermal Cells ◽  
Vitro Study ◽  
Full Thickness ◽  
Thickness Skin ◽  
Skin Biopsies

This in vitro study investigated the possibility of delivering epidermal cells to cell culture plates using a simple and inexpensive aerosolization apparatus. Full-thickness skin biopsies were incubated in dispase to separate the epidermis from the dermis, and the epidermis was treated with trypsin to separate the epidermal cells from one another. The cells were suspended in aerosolization medium (1x106 cells/mL) and sprayed onto culture plates using a sterilized pump-action aerosol nozzle. The plates were incubated and microscopically examined on a daily basis. The aerosolization process was successful in consistently delivering a uniform distribution of suspended epidermal cells. By day 4 there was evidence of cell proliferation, and by days 7 to 9 a confluent layer of cells was achieved on the plates. The monolayer consisted primarily of keratinocytes interspersed with a few fibroblasts. The aerosol method was shown to be effective at delivering a suspension of viable epidermal cells to a culture plate.

scholarly journals In vitro study on release of thyroid hormone in solitary autonomously functioning thyroid nodules using cell culture method.

1984 ◽  
Vol 31 (6) ◽  
pp. 749-753 ◽  
Author(s):  
AKIRA SUGENOYA ◽  
YOSHITSUGU YAMADA ◽  
GENGO KANEKO ◽  
MASARU KOBAYASHI ◽  
MAKOTO MIYAKAWA ◽  
...  
Keyword(s):  
Cell Culture ◽  
Thyroid Hormone ◽  
Thyroid Nodules ◽  
In Vitro Study ◽  
Culture Method ◽  
Vitro Study ◽  
Autonomously Functioning Thyroid Nodules

Screening of the essential oil effects on human H1N1 influenza virus infection: an in vitro study in MDCK cells

2021 ◽  
pp. 1-4
Author(s):  
Basma Najar ◽  
Valeria Nardi ◽  
Maria Alfreda Stincarelli ◽  
Samuele Patrissi ◽  
Luisa Pistelli ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Virus Infection ◽  
Essential Oil ◽  
Mdck Cells ◽  
Influenza Virus Infection ◽  
In Vitro Study ◽  
H1n1 Influenza ◽  
Vitro Study ◽  
H1n1 Influenza Virus

Modulation by Dipyridamole of the Arachidonic Acid Metabolic Pathway in Platelets an in vivo and in vitro Study

1979 ◽  
Author(s):  
Neri G.G. Serneri ◽  
G. F. Gensini ◽  
R. Abbate ◽  
S. Favilla ◽  
R. Laureano
Keyword(s):  
Arachidonic Acid ◽  
Antiplatelet Agent ◽  
In Vitro Study ◽  
Inhibitory Effect ◽  
Oral Route ◽  
Radiometric Assay ◽  
Venous Infusion ◽  
Clinical Conditions

Dipyridamole is a useful antiplatelet agent in specific clinical conditions, but its effects on TxEL production by platelets are now being debated, Resting platelets from patients with 1.5-2 fig/ml serum dipyridamole (spectrofluorimetric assay). administered by venous infusion or by oral route, showed an increased concentration (m.v. +60% P<0.001) of cAMP (radiometric assay). After stimulation with thrombin (5U/ml) platelets produced a significantly decreased amount of TxB(m.v. -60%, F< 0.001) (radioimmunoassay with antibody kindly supplied by Doctor J.B. Smith, Philadelphia). However also after stimulation with arachidonic acid (A.A.) 1 mM TxB production was decreased(m.v. -50%, P<0.001). The incubation of control platelets with different concentrations of dipyridamole (0.5, 1 and 2 μg/ml) for 20 min at 37°C resulted in an increase of cAMP and in a decrease of TxB, production after stimulation with thrombin and with A.A.. These results indicate that dipyridamole is endowed with direct antiaggrega= ting activity caused by a decreased production of TxB2. This in tum seems due to an inhibitory modulating effect of cAMP on arachidonic acid cyclo-oxygenation. However our findings do not rule out an inhibitory effect also on phospholipase A2.

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