scholarly journals Molecular Characterization of Microbiota Associated With Sperm of Malaysian Mahseer Tor tambroides

2019 ◽  
Vol 15 ◽  
pp. 117693431985082
Author(s):  
Ivan Chong Chu Koh ◽  
Bin Hassan Badrul Nizam ◽  
Yazid Muhammad Abduh ◽  
Ambok Bolong Abol Munafi ◽  
Shumpei Iehata
Keyword(s):  
Gel Electrophoresis ◽  
Pathogenic Bacteria ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Sperm Quality ◽  
Good Prospect ◽  
Chain Reaction ◽  
Gradient Gel Electrophoresis ◽  
Polymerase Chain ◽  
Staphylococcus Spp

Malaysian Mahseer ( Tor tambroides) is considered as a good prospect for aquaculture in Malaysia. However, knowledge about Malaysian Mahseer-associated sperm microbiota is still limited, although some studies reported that sperm-related bacteria are a factor in the decline of sperm quality, as sperm may become the carrier of pathogenic bacteria to the egg. The goal of this study was to evaluate the sperm microbiota associated with Malaysian Mahseer from 3 different locations (Universiti Malaysia Terengganu [UMT], Ajil, and Pahang) using polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) fingerprinting and to compare location differences by cluster analysis. Our results showed that the UMT sample had different sperm microbiota composition and a different trend in its relationship with sperm quality. Correlation analysis showed a relationship between bacterial diversity and sperm quality. Phylogenetic analysis indicated that sperm microbiota was composed of diverse phyla, including Proteobacteria, Firmicutes, and Actinobacteria. Interestingly, bacteria such as Salinisphaera sp., Pelomonas sp., and Staphylococcus spp. were detected in all the locations, suggesting that these bacteria are indigenous bacterial members of the Malaysian Mahseer sperm microbiota, although their function is still unclear.

Archaeal characterization of bioaerosols from cage-housed and floor-housed poultry operations

2013 ◽  
Vol 59 (1) ◽  
pp. 46-50 ◽  
Author(s):  
Natasha Just ◽  
Pascale Blais Lecours ◽  
Mélissa Marcoux-Voiselle ◽  
Shelley Kirychuk ◽  
Marc Veillette ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Respiratory Symptoms ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Methanogenic Archaea ◽  
Chain Reaction ◽  
Poultry Facilities ◽  
Gradient Gel Electrophoresis ◽  
Personal Sampling ◽  
Polymerase Chain

Although bioaerosols from both cage-housed (CH) and floor-housed (FH) poultry operations are highly concentrated, the concentrations of dust, endotoxin, and bacteria are significantly higher in FH bioaerosols. Workers from CH operations have reported a greater prevalence of respiratory symptoms. To date, archaea have been examined in swine and dairy bioaerosols but not in poultry bioaerosols. The objective of this study was to directly compare methanogenic archaea concentrations in bioaerosols from CH and FH poultry facilities. Bioaerosols were collected from 15 CH and 15 FH poultry operations, using stationary area samplers as well as personal sampling devices. Archaea were quantified and their diversity was investigated using polymerase chain reaction (PCR) followed by denaturing gradient gel electrophoresis (DGGE) and band sequencing. Archaea were significantly higher in area and personal bioaerosols of CH poultry operations than in those from FH poultry operations (p < 0.001 and p < 0.05, respectively) and did not differ significantly between area and personal samples within each barn type. Sequences matching Methanobrevibacter woesei, an archaea previously found in poultry samples, were detected in bioaerosol samples from CH operations. Methanogenic archaea concentrations are significantly different between bioaerosols from CH and FH poultry operations.

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