scholarly journals DIFFERENTIAL STAINING OF ULTRATHN SECTIONS OF EPON-EMBEDDED TISSUES FOR LIGHT MICROSCOPY

1965 ◽  
Vol 13 (7) ◽  
pp. 579-582 ◽  
Author(s):  
BERNARD P. LANE ◽  
DOMINIC L. EUROPA
Keyword(s):  
Fine Structure ◽  
Periodic Acid ◽  
Differential Staining ◽  
Tissue Architecture ◽  
Paraffin Sections ◽  
Periodic Acid Schiff ◽  
Thin Sections ◽  
Hematoxylin Staining ◽  
Ultrathin Sections ◽  
Hematoxylin Eosin

A convenient method is described for the removal of Epon 812 from thin sections, utilizing a saturated solutions of sodium hydroxide. The tissue architecture and ultrastructural details are preserved. Hematoxylin-eosin, periodic acid-Schiff and phosphotungstic-hematoxylin staining modifications are suggested which result in differentiation similar to that seen in paraffin sections. The technique is applicable to ultrathin sections suitable for examination with the electron microscope, allowing comparison of staining characteristics and fine structure of adjacent thin sections.

Identification of Glycogen in Thin Sections of Amphibian Embryos

1967 ◽  
Vol 2 (2) ◽  
pp. 257-264
Author(s):  
MARGARET M. PERRY
Keyword(s):  
Staining Method ◽  
Periodic Acid ◽  
Differential Staining ◽  
Small Particles ◽  
Periodic Acid Schiff ◽  
Thin Sections ◽  
Enzymic Digestion ◽  
Cell Structures ◽  
Schiff Reaction ◽  
Glycogen Particles

Embryonic amphibian cells when examined with the electron microscope were observed to contain an abundance of small particles, approximately 325 Å in diameter. The periodic acid/Schiff reaction and enzymic digestion were employed to determine the nature of the particles, and from the results of these tests they were concluded to be glycogen. Treatment of thin sections with periodic acid/lead citrate solutions resulted in a marked increase in contrast of the glycogen particles compared with other cell structures, and in a clearly defined substructure of 40-Å grains appearing within the particles. This differential staining method enabled the particulate glycogen to be distinguished from ribosomes.

scholarly journals THE FINE STRUCTURE OF CEROID IN HUMAN ATHEROMA

1967 ◽  
Vol 15 (12) ◽  
pp. 732-736 ◽  
Author(s):  
FERENC GYÖRKEY ◽  
TETSUO SHIMAMURA ◽  
ROBERT M. O'NEAL
Keyword(s):  
Fine Structure ◽  
Electron Microscope ◽  
Lamellar Structure ◽  
Periodic Acid ◽  
Periodic Acid Schiff ◽  
Histochemical Tests ◽  
Ultrathin Sections ◽  
Human Atheroma ◽  
Oil Red O ◽  
Electron Lucent

Ceroid in human atherosclerotic aorta was identified with histochemical tests (acid-fast, oil red O and periodic acid-Schiff techniques) applied to Epon-Araldite- and methacrylate-embedded tissues from which immediately adjacent ultrathin sections were studied with the electron microscope. The fine structure of ceroid in the human atherosclerotic aorta appears to be heterogeneous, showing uniquely wavy, irregularly arranged lamellae lying within granular material of varying electron density. The lamellar structure shows regular and alternating parallel electron-dense and electron-lucent zones which we believe to be characteristic of ceroid.

Ultrastructure of the Parotid Gland of the Nine-Banded Armadillo

1977 ◽  
Vol 35 ◽  
pp. 640-641
Author(s):  
J. R. Ruby
Keyword(s):  
Endoplasmic Reticulum ◽  
Parotid Gland ◽  
Periodic Acid ◽  
Acinar Cells ◽  
Duct System ◽  
Parotid Glands ◽  
Dasypus Novemcinctus ◽  
Anterior Portion ◽  
Periodic Acid Schiff ◽  
Thin Sections

Parotid glands were obtained from five adult (four male and one female) armadillos (Dasypus novemcinctus) which were perfusion-fixed. The glands were located in a position similar to that of most mammals. They extended interiorly to the anterior portion of the submandibular gland.In the light microscope, it was noted that the acini were relatively small and stained strongly positive with the periodic acid-Schiff (PAS) and alcian blue techniques, confirming the earlier results of Shackleford (1). Based on these qualities and other structural criteria, these cells have been classified as seromucous (2). The duct system was well developed. There were numerous intercalated ducts and intralobular striated ducts. The striated duct cells contained large amounts of PAS-positive substance.Thin sections revealed that the acinar cells were pyramidal in shape and contained a basally placed, slightly flattened nucleus (Fig. 1). The rough endoplasmic reticulum was also at the base of the cell.

scholarly journals Morphological and Histological Features of the Vomeronasal Organ in African Pygmy Hedgehog (Atelerix albiventris)

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1462
Author(s):  
Daisuke Kondoh ◽  
Yusuke Tanaka ◽  
Yusuke K. Kawai ◽  
Takayuki Mineshige ◽  
Kenichi Watanabe ◽  
...  
Keyword(s):  
Soft Tissues ◽  
Periodic Acid ◽  
Vomeronasal Organ ◽  
Sensory Epithelium ◽  
Middle Part ◽  
Periodic Acid Schiff ◽  
Histological Features ◽  
Duct Opening ◽  
Hematoxylin Eosin ◽  
Rostral Part

The vomeronasal organ (VNO) detects specific chemicals such as pheromones and kairomones. Hedgehogs (Eulipotyphla: Erinaceidae) have a well-developed accessory olfactory bulb that receives projections from the VNO, but little is known about the hedgehog VNO. Here, we studied the histological features of the VNO in five individual African pygmy hedgehogs by hematoxylin-eosin, periodic acid-Schiff, and Alcian blue stains. The hedgehog VNO comprises a hyaline cartilage capsule, soft tissue and epithelial lumen, and it branches from the site just before the incisive duct opening into the nasal cavity. The soft tissues contain several small mucous (or mucoserous) glands and a large serous gland, and many venous sinuses all around the lumen. The VNO lumen is round to oval throughout the hedgehog VNO, and the sensory epithelium lines almost the entire rostral part and medial wall of the middle part. These findings indicate that the VNO is functional and plays an important role in the hedgehog. Notably, the VNO apparently has a characteristic flushing mechanism with serous secretions like those of gustatory glands, which the hedgehog might frequently use to recognize the external environment.

Lectins as Cytochemical Probes of the Developing Wheat Grain. V. Demonstration of Separate Polysaccharides Containing N-Acetyl-D-Glucosamine and D-Galactose in Nuclear Epidermal Cell Walls

1984 ◽  
Vol 11 (3) ◽  
pp. 179 ◽  
Author(s):  
BA Baldo ◽  
D Barnett ◽  
JW Lee
Keyword(s):  
Epidermal Cell ◽  
Cell Walls ◽  
Wheat Germ ◽  
Periodic Acid ◽  
Fluorescein Isothiocyanate ◽  
Wheat Grain ◽  
Periodic Acid Schiff ◽  
Thin Sections ◽  
Lectin Staining ◽  
Wheat Germ Lectin

Fluorescein isothiocyanate-labelled lectin from wheat-gem, which binds N-acetyl-D-glucosamine, and Griffonia simplicifolia, Arachis hypogaea and Glycine max lectins, each of which binds D-galactose, react with nucellar epidermal cell walls in thin sections of plastic-embedded developing wheat grain. Reactivity of these cell walls with periodic acid-Schiff reagent, the absence of staining with protein stains and the failure of a number of proteases and the endoglycosidases D and H to prevent the binding suggested that the lectin-reactive wall components are neither proteins nor N-glycosidically linked glycoproteins. Morphological differences in lectin staining patterns and treatment of sections with chitinase and α-galactosidase, prior to the reaction with the lectins, indicated that two separate polysaccharides are probably involved in the binding. Chitinase removed the reactivity of the nucellar epidermal cell walls for wheat-germ lectin but the binding of D-galactose-specific lectins was unimpaired. Conversely, α-galactosidase did not affect the binding of wheat-germ lectin but reactivity with the galactose-specific lectins was abolished. From the available evidence we conclude that one polysaccharide in the nucellar epidermal cell wall reacts with wheat-germ lectin and contains N-acetyl-D-glucosamine in a chitin-like structure. The other polysaccharide reacts with D-galactose- specific lectins by virtue of terminal α-D-galactose residues. Hydrolysis and subsequent chromatographic analysis of nucellar epidermal cell walls peeled from immature grains revealed the presence of D-glucosamine, D-glucose, D-galactose, D-xylose, L-arabinose and a trace of D-mannose.

scholarly journals Actinomycosis is not Frequent in the Periapex But is a Persistent Lesion

2017 ◽  
Vol 28 (6) ◽  
pp. 688-693 ◽  
Author(s):  
Lucas Senhorinho Esteves ◽  
Águida Cristina Gomes Henriques ◽  
Carolina Ávila Varginha de Moraes e Silva ◽  
Maria Cristina Teixeira Cangussu ◽  
Eduardo Antônio Gonçalves Ramos ◽  
...  
Keyword(s):  
Differential Diagnosis ◽  
Periodic Acid ◽  
Lesion Size ◽  
Periapical Lesion ◽  
Periodic Acid Schiff ◽  
Bacterial Colony ◽  
Periapical Lesions ◽  
Criteria For Diagnosis ◽  
Hematoxylin Eosin

Abstract Periapical actinomycosis caused by a gram-positive anaerobic pathogen characterizes a typical extra-radicular infection. This study determined the frequency and correlated the content of bacteria colonies with the of periapical actinomycosis size. The study comprised a total of 218 periapical lesions (PL) (cysts, granulomas or abscess). The specimens embedded in paraffin were sliced into 4-µm sections and stained with hematoxylin-eosin, Gram, Periodic Acid-Schiff (PAS) and Grocott’s stain. The presence of bacterial colonies composed of filamentous structures labeled with the histochemical stains were described as Actinomyces, and for each case, the bacterial colonies were counted and measured. The correlation between the number and size of bacterial colonies and the size of PL was tested using Pearson’s adjusted correlation coefficient. From 218 PL, bacterial colonies were identified in 64 biopsies. Seven cases (0.3%) fulfill the criteria for diagnosis of periapical actinomycosis. All of cases were therapy-resistant and did not showed periapical repair after 12 months of follow-up. Periapical surgery or dental extraction was performed. The correlation test indicated no correlation between the number of bacterial colonies and the lesion size (p=0.752, r=-0.148). However, a larger bacterial colony size generally resulted in a larger periapical lesion (P=0.000, r=0.657). The frequency of periapical actinomycosis was low, and this lesion should be included in the differential diagnosis of PL. The size of the Actinomyces colonies seemed to contribute to increase the size of the periapical lesion.

scholarly journals Relationship between the morphologic alterations of vocal cords from adult autopsies and the cause of death

2004 ◽  
Vol 59 (2) ◽  
pp. 63-66 ◽  
Author(s):  
Ana Karina Marques Salge ◽  
Eumenia Costa da Cunha Castro ◽  
Mara Lúcia Fonseca Ferraz ◽  
Marlene Antônia dos Reis ◽  
Vicente de Paula Antunes Teixeira
Keyword(s):  
Vocal Cord ◽  
Cause Of Death ◽  
Respiratory Diseases ◽  
Periodic Acid ◽  
Basal Membrane ◽  
Vocal Cords ◽  
Periodic Acid Schiff ◽  
White Ethnicity ◽  
Staining Methods ◽  
Hematoxylin Eosin

PURPOSE: The purpose of this study was to identify the possible alteration in the thickness of the epithelium basal membrane of the vocal cords and correlate it with the cause of death. METHOD: Larynxes collected from adult autopsies during the period of 1993 to 2001 were utilized. We used the hematoxylin-eosin and periodic acid-Schiff staining methods for the morphological and morphometric analysis. RESULTS: Sixty-six vocal cords were analysed; increased thickness was identified in 14 cases (21.2%), with equal proportions between the genders. Increased vocal-cord thickness was more frequent in patients of the white ethnicity (12 cases, 85.7%). Respiratory alterations were found in 10 (71.4%) of the cases with increased vocal-cord thickness. Of the patients that were maintained with mechanical ventilation before death, 7 (18.4%) had thickening of the basal membrane. Among the smokers, 9 (19.63%) had basal membrane thickening. CONCLUSION: No statistically significant differences were found between the cases in which the cause of death was related to respiratory diseases as compared to non-respiratory diseases and the thickening of the basal membrane of the vocal cords. However, new studies are needed in order to verify the etiopathogenesis of this thickening.

PATHOLOGY AND RENAL OUTCOME OF THREE UNCOMMON FACES OF CRESCENTRIC GLOMERULONEPHRITIS

2021 ◽  
pp. 7-12
Author(s):  
Keya Basu ◽  
Dipankar Sircar ◽  
Manimoy Bandopadhyay
Keyword(s):  
Iga Nephropathy ◽  
Survival Data ◽  
Periodic Acid ◽  
Rapidly Progressive Glomerulonephritis ◽  
Renal Outcome ◽  
Single Centre ◽  
Periodic Acid Schiff ◽  
Cross Sectional ◽  
Single Centre Study ◽  
Hematoxylin Eosin

AIMS: Rapidly progressive glomerulonephritis (RPGN) presents with rapidly deteriorating renal function (> 50% loss of glomerular ltration rate /GFR within 3 months) associated with nephritic urinary sediments and crescents in biopsy. Crescentric IgA Nephropathy, Anti-GBM (Glomerular basement membrane) disease and combined IgA Nephropathy with Anti-GBM disease are three uncommon reasons of RPGN. We have compared clinicopathological. Immunouorescence (DIF) and renal outcome of three groups. Setting and designs: Prospective, cross-sectional, single centre study. METHODS AND MATERIALS :Ultrasonography guided core biopsies obtained, one stained with hematoxylin-eosin, periodic acid-Schiff, Masson's trichrome, and silver methenamine stain another one with immunouorescence conjugated IgG, IgM, IgA, C3, C1q, kappa and lambda stain. Demographic, clinicopathological and therapeutic parameters with survival data were collected. STATISTICAL ANALYSIS USED: Done using software (GraphPad PRISM 6). RESULTS: We have included 9 cases of crescentric IgA Nephropathies, 6 cases of AntiGBM diseases and 2 cases of combined IgA Nephropathy and AntiGBM diseases. Signicant difference seen in the incidences of hypertension, hemoptysis, serum creatinine, anti GBM antibody, total number of crescents and mesangial hypercellularity, fragmentation of GBM etc. IgG, IgA and kappa positivity in DIF show signicant difference. Survival analysis and mortality versus dialysis dependence and complete and partial remission versus no remission showed no difference between these three groups. CONCLUSIONS: Proper and early clinicopathological diagnosis is important since all are of poor renal outcome. Further renal outcome of the combined disease is same as that of individual ones.

scholarly journals An improved periodic acid-thiosemicarbazide-osmium technique to reveal glycoconjugates at the molecular level in situ.

1986 ◽  
Vol 34 (9) ◽  
pp. 1161-1170 ◽  
Author(s):  
M Derenzini ◽  
F Farabegoli ◽  
V Marinozzi
Keyword(s):  
Structural Organization ◽  
Molecular Level ◽  
Periodic Acid ◽  
Basement Membranes ◽  
Staining Reaction ◽  
Membrane Glycoproteins ◽  
Periodic Acid Schiff ◽  
Thin Sections ◽  
Periodic Distribution

The periodic acid-thiocarbohydrazide or thiosemicarbazide-OsO4 method (Seligman AM, Hanker JS, Wasserkrug H, Katzoff L: J Histochem Cytochem 13:629, 1965) has been modified in order to obtain a periodic acid-Schiff (PAS)-like reaction for electron microscopy capable of visualizing structures at the molecular level in situ. Thiocarbohydrazide (TCH) and thiosemicarbazide (TSC) have been used dissolved in distilled water and bubbled with SO2. Treatment of previously oxidized thin sections with TCH (SO2) or TSC (SO2), followed by osmification, resulted in selective and very good staining of all the PAS-positive structures examined: glycogen, intestinal mucopolysaccharides, plasma membrane glycoproteins, basement membranes, Golgi apparatus, and collagen. The staining reaction was highly specific when TSC was used on thin sections from paraformaldehyde-fixed samples. The non-particulate end-reaction product made possible visualization of a periodic distribution of sugar residues in the 64-nm unit of collagen and the structural organization of the PAS-positive glycoconjugate components in the glomerular basement membrane.

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