scholarly journals Role of Bee Venom and Melittin on Restraining Angiogenesis and Metastasis in γ-Irradiated Solid Ehrlich Carcinoma-Bearing Mice

2020 ◽  
Vol 19 ◽  
pp. 153473542094447
Author(s):  
Nermeen M. El Bakary ◽  
Asmaa Z. Alsharkawy ◽  
Zeinab A. Shouaib ◽  
Emad M. S. Barakat
Keyword(s):  
Ehrlich Ascites Carcinoma ◽  
Bee Venom ◽  
Whole Body ◽  
Major Constituent ◽  
Ehrlich Carcinoma ◽  
Γ Radiation ◽  
Ehrlich Ascites ◽  
Tnf Α ◽  
Therapeutic Protocols

Pathological angiogenesis and apoptosis evasion are common hallmarks of cancer. The present work was an endeavor to evaluate the influence of bee venom (BV) or its major constituent melittin (MEL) as antiapoptotic and angiogenic regulator modifier on the tumor growth and the cell sensitivity to ionizing radiation targeting the improvement of cancer therapeutic protocols. BV (0.56 mg/kg/day) and MEL (500 µg/kg body weight/day) were injected intraperitoneally to mice bearing 1 cm3 solid tumor of Ehrlich ascites carcinoma (EAC) for 21 consecutive days. Mice were whole-body exposed to 1 Gray (Gy) of γ-radiation (2 fractionated doses). Treatment with BV or MEL markedly suppresses the proliferation of tumor in EAC mice. The concentrations of m-RNA for angiogenic factors (TNF-α, VEGF) as well as MMPs 2 and 9 activities and NO concentration were significantly decreased, combined with improvements in apoptotic regulators (caspase-3 activity) and normal cells redox tone (catalase and free radicals content) compared with EAC mice. Moreover, the histopathological investigation confirms the improvement exerted by BV or MEL in the EAC mice group or EAC + R group. Exposure to γ-radiation sustained the modulatory effect of BV on tumor when compared with EAC + BV mice. Convincingly, the role of BV or MEL as a natural antiangiogenic in the biological sequelae after radiation exposure is verified. Hence, BV and its major constituent MEL might represent a potential therapeutic strategy for increasing the radiation response of solid tumors.

THE ROLE OF ADENOSINE TRIPHOSPHATE IN TRANSPORT REACTIONS

1964 ◽  
Vol 42 (6) ◽  
pp. 917-924 ◽  
Author(s):  
P. G. Scholefield
Keyword(s):  
Amino Acid ◽  
Adenosine Triphosphate ◽  
Quantitative Relationship ◽  
Ehrlich Ascites Carcinoma ◽  
Amino Acid Uptake ◽  
Ehrlich Carcinoma ◽  
Acid Uptake ◽  
Ehrlich Ascites ◽  
Qualitative Relationship

The evidence bearing on the participation of adenosine triphosphate (ATP) in transport reactions is reviewed. The connection may take the form of a strict quantitative relationship as observed in potassium uptake by red blood cells or as in amino acid uptake by slices of rat brain cortex. There may also be a qualitative relationship such as that observed in studies of amino acid uptake by Ehrlich ascites carcinoma cells. In other tissues (e.g. slices of the Ehrlich carcinoma) amino acid uptake may only have an uncertain relationship to the ATP content of the tissue. Some of the schemes which have been put forward to account for the involvement of ATP are discussed.

THE EFFECT OF LETHAL DOSES OF X-IRRADIATION ON THE ENZYMATIC ACTIVITY OF MITOCHONDRIAL CYTOCHROME c

1966 ◽  
Vol 44 (4) ◽  
pp. 433-448 ◽  
Author(s):  
J. F. Scaife
Keyword(s):  
Electron Transport ◽  
Cytochrome C ◽  
Electron Transport Chain ◽  
Reductase Activity ◽  
Ehrlich Ascites Carcinoma ◽  
Whole Body ◽  
Triphenyltetrazolium Chloride ◽  
Transport Chain ◽  
Ehrlich Ascites ◽  
X Irradiation

The coupling of the tetrazolium salts triphenyltetrazolium chloride and nitro-blue tetrazolium to the electron transport chain in mitochondria of thymus, spleen, liver, kidney, and Ehrlich ascites carcinoma cells has been studied with several substrates. In experiments on succinate–triphenyltetrazolium chloride reductase activity it has been possible to demonstrate a radiation lesion in the electron transport chain of mitochondria from thymus and spleen, but not in those from other tissues. This lesion is evident 4 hours after 25 rad of whole-body irradiation, or earlier with higher doses. It is not prevented by the prior administration of aminoethylisothiouronium bromide, serotonin, vitamin K1, or vitamin E, but is reduced by anoxic conditions.Lower levels of cytochrome c are found in irradiated mitochondria isolated from thymus, and the radiation lesion is believed to be produced by loosening the binding of cytochrome c to the mitochondrial membrane after X-irradiation. Decreased levels of ATP occur in thymus, spleen, and ascites cells following irradiation.

Inhibition of nuclear DNA-dependent RNA polymerases from mouse ascites tumors and liver by glycerol

1977 ◽  
Vol 55 (10) ◽  
pp. 1117-1120 ◽  
Author(s):  
D. G. R. Blair
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Mouse Liver ◽  
Nuclear Dna ◽  
Ehrlich Ascites Carcinoma ◽  
Rna Polymerases ◽  
Ehrlich Carcinoma ◽  
Ehrlich Ascites ◽  
Mouse Ascites ◽  
Ascites Tumors

Nuclear DNA-dependent RNA polymerases were isolated from Ehrlich ascites carcinoma, TA3 ascites adenocarcinoma, and mouse liver and tested for inhibition by glycerol. The results confirm the finding of Smith and Duerksen ((1975) Biochem. Biophys. Res. Commun. 67, 916–923) that glycerol may inhibit nuclear RNA polymerase II, but because different grades of glycerol inhibited mouse liver RNA polymerase IIa to different extents, it is suggested that an inhibitory contaminant is present. RNA polymerases IIa and IIb from the two tumors and mouse liver were proportionately inhibited by A.C.S. reagent-grade glycerol at concentrations above 10%. RNA polymerase Ia from liver and the TA3 tumor was not inhibited by any concentration of glycerol tested (2–32.3%), but RNA polymerase Ia from Ehrlich carcinoma was inhibited by glycerol concentrations above 16%.

scholarly journals Anticancer redox activity of gallium nanoparticles accompanied with low dose of gamma radiation in female mice

Tumor Biology ◽  
2018 ◽  
Vol 40 (3) ◽  
pp. 101042831774967 ◽  
Author(s):  
Eman I Kandil ◽  
Sawsan M El-sonbaty ◽  
Fatma SM Moawed ◽  
Ola MS Khedr
Keyword(s):  
Gamma Radiation ◽  
Alanine Aminotransferase ◽  
Low Dose ◽  
Whole Body ◽  
Ehrlich Carcinoma ◽  
Γ Radiation ◽  
Female Mice ◽  
Gallium Nanoparticles ◽  
Solid Ehrlich Carcinoma ◽  
Iron Concentrations

Guided treatments with nanoparticles and radiotherapy are a new approach in cancer therapy. This study evaluated the beneficial antitumor effects of γ-radiation together with gallium nanoparticles against solid Ehrlich carcinoma in female mice. Gallium nanoparticles were biologically synthesized using Lactobacillus helveticus cells. Transmission electron microscopy showed gallium nanoparticles with size range of 8−20 nm. In vitro study of gallium nanoparticles on MCF-7 revealed IC50 of 8.0 μg. Gallium nanoparticles (0.1 mg/kg body weight) were injected intraperitoneally daily on the seventh day of Ehrlich carcinoma cells inoculation. Whole-body γ-radiation was carried out at a single dose of 0.25 Gy on eighth day after tumor inoculation. Biochemical analysis showed that solid Ehrlich carcinoma induced a significant increase in alanine aminotransferase activity and creatinine level in serum, calcium, and iron concentrations in liver tissue compared to normal control. Treatment of Ehrlich carcinoma–bearing mice with gallium nanoparticles and/or low dose of γ-radiation exposure significantly reduced tumor volume, decreased alanine aminotransferase and creatinine levels in serum, increased lipid peroxidation, and decreased glutathione content as well as calcium and iron concentrations in liver and tumor tissues with intense DNA fragmentation accompanied compared to untreated tumor cells. Moreover, mitochondria in the treated groups displayed a significant increase in Na+/K+-ATPase, complexes II and III with significant reduction in CYP450 gene expression, which may indicate a synergistic effect of gallium nanoparticles and/or low dose of γ-radiation combination against Ehrlich carcinoma injury, and this results were well appreciated with the histopathological findings in the tumor tissue. We conclude that combined treatment of gallium nanoparticles and low dose of gamma-radiation resulted in suppressive induction of cytotoxic effects on cancer cells.

Effects of Lysozyme on Ehrlich Ascites Carcinoma Cells: The Possible Physiological Role of Lysozyme in the Prevention of Carcinogenesis

2019 ◽  
Vol 5 (2) ◽  
pp. 113-118
Author(s):  
Vardan K. Gasparyan ◽  
Hayk A. Harutyunyan ◽  
Mariam V. Mikaelyan ◽  
Gayane G. Poghosyan
Keyword(s):  
Physiological Role ◽  
Ehrlich Ascites Carcinoma ◽  
Carcinoma Cells ◽  
Ehrlich Ascites

scholarly journals ANTIOXIDANT, METABOLIC AND ANTITUMOR ACTIVITY OF TRITERPENOIDS COMBINATION WITH CYTOSTATICS

2020 ◽  
pp. 49-56
Author(s):  
OLGA VOROBYOVA ◽  
ELIZAVETA GRUBOVA ◽  
KSENIYA BELYAEVA ◽  
ANNA SOLOVYEVA ◽  
NADEZHDA PLOTNIKOVA ◽  
...  
Keyword(s):  
Antioxidant Properties ◽  
Ehrlich Ascites Carcinoma ◽  
Ehrlich Carcinoma ◽  
Ros Generation ◽  
Rat Blood ◽  
Ldh Activity ◽  
Ehrlich Ascites ◽  
Combination Drugs ◽  
Betulin Derivatives

Objective: To study the effect of betulin derivatives combination with 5-fluorouracil or hydrazine sulfate on the ROS generation, the SOD and LDH activity using rat blood, as well as the effect of combination drugs on Ehrlich carcinoma in experiments on mice. Methods: We used a chemiluminescence technique to study the ROS generation, and spectrophotometry to determine the MDA level and the SOD and LDH activity. The model of transplanted Ehrlich ascites carcinoma was investigated on mice using a cytological analysis of ascitic fluid cells according to Pappenheim`s method. Results: In vitro experiments on rat blood at the doses of 2, 5 and 10 μg per ml revealed the dose-dependent effect of combination drugs on the antioxidant properties. In plasma, the ROS generation and the MDA level increased by 10-300% in comparison with control at the doses of 5 and 10 μg per ml only. Still, the SOD and LDH activity in general increased by 10-130% in comparison with control under the action of the studied combination drugs. The study on mice showed the effectiveness of a combination of triterpenoids and cytostatics in Ehrlich ascites carcinoma therapy. The state and behavior of the animals improved, the volume of ascites fluid decreased by 40-50% after treatment for 10 d. Conclusion: The combination of betulin derivatives with cytostatics can be used as antitumor drugs in Ehrlich ascites carcinoma therapy that is due to metabolic plasticity, increased ROS generation in enhanced antioxidant enzyme protection.

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