Long non-coding RNA HULC affects the proliferation, apoptosis, migration, and invasion of mesenchymal stem cells

Further studies on the molecular mechanisms of mesenchymal stem cells in the maintenance of growth and function are essential for their clinical application. Growing evidence has shown that long non-coding RNAs (lncRNAs) play an important role in the regulation of mesenchymal stem cells. Recently, it is reported that highly upregulated in liver cancer (HULC), with another lncRNA MALAT-1, accelerated liver cancer stem cell growth. The regulating role of MALAT-1 in mesenchymal stem cells has been investigated. However, the effects of HULC on the mesenchymal stem cells are unknown. In this study, we overexpressed HULC in mesenchymal stem cells derived from umbilical cord and analyzed the cell phenotypes, proliferation, apoptosis, migration, invasion and differentiation of mesenchymal stem cells. We found that overexpression of HULC significantly promotes cell proliferation through promoting cell division and inhibits cell apoptosis. HULC-overexpressed mesenchymal stem cells migrate and invade faster than control mesenchymal stem cells. HULC has no effect on phenotypes and differentiation of mesenchymal stem cells. Furthermore, we found that the expression of HULC in mesenchymal stem cells could be reduced by several inflammatory factors, including TNF-α, TGF-β1, and R848. Taken together, our data demonstrated that HULC has a vital role in the growth and function maintenance of mesenchymal stem cells without affecting differentiation. Impact statement Exploring the molecular mechanisms of growth and function in MSCs is the key to improve their clinical therapeutic effects. Currently, more and more evidence show that the long non-coding RNA (lncRNA) plays an important role in the growth, stemness and function of MSCs.Both HULC and MALAT1 are the earliest discovered LNCRNAs, which are closely related to tumor growth. All of them can promote the growth of liver cancer stem cells. Previously, we have studied the effects of MALAT1 on the growth and function of MSCs. In this study, we focused on the effects of HULC on MSCs. We elucidated the effects of HULC on the growth and differentiation of MSCs, and explored the relationship between inflammatory stimuli and HULC expression in MSCs. Our findings provide a new molecular target for the growth and clinical application of MSCs.

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The Cross-Talk between Mesenchymal Stem Cells and Immune Cells in Tissue Repair and Regeneration

International Journal of Molecular Sciences ◽

10.3390/ijms22052472 ◽

2021 ◽

Vol 22 (5) ◽

pp. 2472

Author(s):

Carl Randall Harrell ◽

Valentin Djonov ◽

Vladislav Volarevic

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Immune Cells ◽

Tissue Repair ◽

Molecular Mechanisms ◽

Current Knowledge ◽

Multipotent Stem Cells ◽

Tissue Repair And Regeneration ◽

Almost All ◽

And Function

Mesenchymal stem cells (MSCs) are self-renewable, rapidly proliferating, multipotent stem cells which reside in almost all post-natal tissues. MSCs possess potent immunoregulatory properties and, in juxtacrine and paracrine manner, modulate phenotype and function of all immune cells that participate in tissue repair and regeneration. Additionally, MSCs produce various pro-angiogenic factors and promote neo-vascularization in healing tissues, contributing to their enhanced repair and regeneration. In this review article, we summarized current knowledge about molecular mechanisms that regulate the crosstalk between MSCs and immune cells in tissue repair and regeneration.

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Long non-coding RNA BDNF-AS modulates osteogenic differentiation of bone marrow-derived mesenchymal stem cells

Molecular and Cellular Biochemistry ◽

10.1007/s11010-017-3251-2 ◽

2017 ◽

Vol 445 (1-2) ◽

pp. 59-65 ◽

Cited By ~ 10

Author(s):

Xiaobo Feng ◽

Tao Lin ◽

Xianzhe Liu ◽

Cao Yang ◽

Shuhua Yang ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Osteogenic Differentiation ◽

Non Coding Rna ◽

Long Non Coding Rna

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Long non-coding RNA, Bmcob, regulates osteoblastic differentiation of bone marrow mesenchymal stem cells

Biochemical and Biophysical Research Communications ◽

10.1016/j.bbrc.2018.09.142 ◽

2018 ◽

Vol 506 (3) ◽

pp. 536-542 ◽

Cited By ~ 6

Author(s):

Xi Sun ◽

Ying Yuan ◽

Ye Xiao ◽

Qiong Lu ◽

Li Yang ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Osteoblastic Differentiation ◽

Non Coding Rna ◽

Marrow Mesenchymal Stem Cells ◽

Long Non Coding Rna

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Correction: Long non-coding RNA HoxA-AS3 interacts with EZH2 to regulate lineage commitment of mesenchymal stem cells

Oncotarget ◽

10.18632/oncotarget.25078 ◽

2018 ◽

Vol 9 (25) ◽

pp. 17978-17978 ◽

Cited By ~ 1

Author(s):

Xin-Xing Zhu ◽

Ya-Wei Yan ◽

Demeng Chen ◽

Chun-Zhi Ai ◽

Xifeng Lu ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Lineage Commitment ◽

Non Coding Rna ◽

Long Non Coding Rna

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Long non‑coding RNA BCAR4 promotes liver cancer progression by regulating proliferation, migration and invasion

Oncology Letters ◽

10.3892/ol.2020.11826 ◽

2020 ◽

Vol 20 (3) ◽

pp. 2779-2787

Author(s):

Aiyao Wang ◽

Jun Meng ◽

Hui Liu ◽

Chen Li ◽

Zhiyong Zhou

Keyword(s):

Liver Cancer ◽

Cancer Progression ◽

Migration And Invasion ◽

Non Coding Rna ◽

Long Non Coding Rna

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TUG1 promotes prostate cancer progression by acting as a ceRNA of miR-26a

Bioscience Reports ◽

10.1042/bsr20180677 ◽

2018 ◽

Vol 38 (5) ◽

Cited By ~ 18

Author(s):

Bin Yang ◽

Xiaodi Tang ◽

Zhixin Wang ◽

Daju Sun ◽

Xin Wei ◽

...

Keyword(s):

Prostate Cancer ◽

Cancer Progression ◽

Migration And Invasion ◽

Tumor Cell Migration ◽

Real Time Quantitative Pcr ◽

Non Coding Rna ◽

Key Aspects ◽

And Function ◽

First Time ◽

Long Non Coding Rna

Previous studies have demonstrated that taurine-upregulated gene 1 (TUG1) was aberrantly expressed and involved in multiple types of cancer; however, the expression profile and potential role of TUG1 in prostate cancer (PCa) remains unclear. The aim of the present study was to evaluate the expression and function of TUG1 in PCa. In the present study, we analyzed TUG1 expression levels of PCa patients in tumor and adjacent normal tissue by real-time quantitative PCR. Knockdown of TUG1 by RNAi was performed to explore its roles in cell proliferation, migration, and invasion. Here we report, for the first time, that TUG1 promotes tumor cell migration, invasion, and proliferation in PCa by working in key aspects of biological behaviors. TUG1 could negatively regulate the expression of miR-26a in PCa cells. The bioinformatics prediction revealed putative miR-26a-binding sites within TUG1 transcripts. In conclusion, our study suggests that long non-coding RNA (lncRNA) TUG1 acts as a functional oncogene in PCa development.

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The Role of Tumor-related LncRNA PART1 in cancer

Current Pharmaceutical Design ◽

10.2174/1381612827666210705161955 ◽

2021 ◽

Vol 27 ◽

Author(s):

Jinlan Chen ◽

Enqing Meng ◽

Yexiang Lin ◽

Yujie Shen ◽

Chengyu Hu ◽

...

Keyword(s):

Molecular Mechanisms ◽

Malignant Tumors ◽

Migration And Invasion ◽

Signal Pathways ◽

Toll Like Receptor ◽

Non Coding Rna ◽

Regulated Expression ◽

The One ◽

Long Non Coding Rna

Background: As we all know, long non-coding RNA (lncRNA) affects tumor progression, which has caused a great upsurge in recent years. It can also affect the growth, migration, and invasion of tumors. When we refer to the abnormal expression of lncRNA, we will find it associated with malignant tumors. In addition, lncRNA has been proved to be a key targeted gene for the treatment of some diseases. PART1, a member of lncRNA, has been reported as a regulator in the process of tumor occurrence and development. This study aims to reveal the biological functions, specific mechanisms, and clinical significance of PART1 in various tumor cells. Methods: Through the careful search of PUBMED, the mechanisms of the effect of PART1 on tumorigenesis and development are summarized. Results: On the one hand, the up-regulated expression of PART1 plays a tumor-promoting role in tumors, including lung cancer, prostate cancer, bladder cancer and so on. On the other hand, PART1 is down-regulated in gastric cancer, glioma and other tumors to play a tumor inhibitory role. In addition, PART1 regulates tumor growth mainly by targeting microRNA such as miR-635, directly regulating the expression of proteins such as FUS/EZH2, affecting signal pathways such as the Toll-like receptor pathway, or regulating immune cells. Conclusion: PART1 is closely related to tumors by regulating a variety of molecular mechanisms. In addition, PART1 can be used as a clinical marker for the early diagnosis of tumors and plays an important role in tumor-targeted therapy.

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