Enteral nutrition ameliorates the symptoms of Crohn's disease in mice via activating special pro-resolving mediators through innate lymphoid cells

Crohn's disease activates the inflammatory reactions to induce intestinal disorders. Enteral nutrition (EN) could exert general immunomodulatory effects. Cecal ligation and perforation (CLP) surgery was utilized to establish Crohn's disease mice models. Survival analysis, hematoxylin-eosin staining, flow cytometry, ELISA, Western blot and liquid chromatography-tandem MS were applied. Baicalein was added to inhibit lipoxygenases. The survival rate was restored and inflammatory injury, exudate neutrophils in peritoneal lavage and serum levels of IL-6 and TNF-α were ameliorated by EN treatment as compared with CLP treatment. EN also increased ILC-3 content, 5/15-LOX level and RvD1-RvD5 in peritoneal lavage. Baicalein reversed all the detected effects of EN except ILC-3 content. EN could activate special pro-resolving mediators (SPMs) through ILCs to mitigate injuries of Crohn's disease.

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Potential Pro-Inflammatory Role Of VEGF In Patients With Crohn’s Disease

Serbian Journal of Experimental and Clinical Research ◽

10.1515/sjecr-2015-0046 ◽

2015 ◽

Vol 16 (4) ◽

pp. 319-326

Author(s):

Natasa Zdravkovic ◽

Ivan Jovanovic ◽

Gordana Radosavljevic ◽

Nebojsa Zdravkovic ◽

Slobodanka Mitrovic ◽

...

Keyword(s):

Ulcerative Colitis ◽

Immune Response ◽

Crohn’S Disease ◽

Crohn's Disease ◽

Expression Patterns ◽

Serum Levels ◽

Enhanced Production ◽

Tnf Α ◽

Affected Tissue

AbstractThe aim of this study was to investigate the expression patterns of p16, p53 and VEGF in affected tissue and serum levels of cytokines TNF-α, IL-6, TGF-β and IL-17 in patients with ulcerative colitis (UC) and fistulating Crohn’s disease (CD). Serum levels of cytokines in patients with ulcerative colitis (n=24) and with Crohn’s disease (n=7) were analysed by ELISA. In colonoscopically obtained biopsies, p16, p53 and vascular endothelial growth factor expression were evaluated by immunohistochemistry.The results of this study clearly show the predominance of pro-inflammatory type 1 and 17 immune response in patients with CD compared to those with UC. We believe that altered p16 and p53 induce enhanced VEGF expression and implicates enhanced production of pro-inflammatory TNF-α and IL-6. TNF-α and IL-6 further facilitate development of type 1/17 immune response.

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Predictive factors of response to anti-TNF α treatment of complex ano-perineal fistulas in Crohn’s disease.

10.26226/morressier.59a6b34cd462b80290b55a24 ◽

2017 ◽

Author(s):

maroua hafi

Keyword(s):

Crohn’S Disease ◽

Crohn's Disease ◽

Predictive Factors ◽

Tnf Α

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Faculty Opinions recommendation of Crohn's disease exclusion diet plus partial enteral nutrition induces sustained remission in a randomized controlled trial.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.735919047.793568128 ◽

2019 ◽

Author(s):

Ashish Patel

Keyword(s):

Randomized Controlled Trial ◽

Crohn’S Disease ◽

Crohn's Disease ◽

Enteral Nutrition ◽

Controlled Trial ◽

Sustained Remission ◽

Randomized Controlled ◽

Exclusion Diet

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Meta-analysis of enteral nutrition as a primary treatment of active crohn's disease

Gastroenterology ◽

10.1016/0016-5085(95)90203-1 ◽

1995 ◽

Vol 108 (4) ◽

pp. 1056-1067 ◽

Cited By ~ 316

Author(s):

Anne M. Griffiths ◽

Arne Ohlsson ◽

Philip M. Sherman ◽

Lloyd R. Sutherland

Keyword(s):

Crohn’S Disease ◽

Crohn's Disease ◽

Enteral Nutrition ◽

Meta Analysis ◽

Primary Treatment

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THE EVALUATION OF SERUM SEROTONIN LEVEL AMONG PATIENTS WITH CROHN’S DISEASE AND ITS IMPACT ON QUALITY OF LIFE

Inflammatory Bowel Diseases ◽

10.1093/ibd/izaa347.131 ◽

2021 ◽

Vol 27 (Supplement_1) ◽

pp. S55-S55

Author(s):

Marcin Sochal ◽

Piotr Bialasiewicz ◽

Agata Gabryelska ◽

Renata Talar-Wojnarowska ◽

Jakub Fichna ◽

...

Keyword(s):

Crohn’S Disease ◽

Crohn's Disease ◽

Sleep Quality ◽

Sleep Fragmentation ◽

Clinical Severity ◽

Serotonin Level ◽

Intestinal Physiology ◽

Tnf Α

Abstract Background and aims Serotonin affects intestinal physiology, mood, as well as circadian rhythm. Moreover, serotonin has proinflammatory function. Therefore, the aim of this study was to investigate the role of serotonin in clinical severity of Crohn’s Disease (CD) and its effect on pain and sleep quality. Methods Fifty-nine CD patients (34 in exacerbation and 25 in remission according to the Harvey-Bradshaw Index-HBI) and 25 health control individuals(HC) were recruited. Sleep quality was assessed by the Pittsburgh Sleep Quality Index (PSQI) and subjective severity of pain by the Visual Analog Scale (VAS). Seventeen patients were treated with anti-TNF-α induction therapy for 14 weeks. Results Serotonin level was higher in CD (145.12ng/mL, IQR:98.14–179.25) compared to HC (87.52ng/mL, IQR:70.04–129.39; p=0.002) and in exacerbation of CD (157.66ng/mL, IQR:111.94–197.64) compared to remission (122.33ng/mL, IQR:83.28–163.67; p=0.029). Serotonin level with cut-off point of 92.45 ng/mL is useful for distinguishing participants with CD from HC (sensitivity: 78%, specificity: 60%, positive predictive value: 82%). Positive correlation between serotonin and HBI (r=0.279, p=0.032) and severity of diarrhoea (r=0.260, p=0.047) were found. Serotonin does not correlate with PSQI (r=0.152, p=0.168), but correlates with presence of sleep fragmentation for example by getting up to use the bathroom (joined 5b-5j PSQI questions; r=0.270, p=0.039). Correlations between serotonin and VAS were also obtained (r=0.220, p=0.045). Moreover, serotonin level significantly decreased after anti-TNF-α therapy (192.35ng/mL, IQR:150.36–225.56 vs. 121.11ng/mL, IQR:91.28–188.87; p=0.006). The study was funded by National Science Centre, Poland (#2018/31/N/NZ5/03715). Conclusions Serotonin level correlates with the severity of CD and decreases after anti-TNF-α therapy. It is associated with sleep fragmentation, which may be caused by diarrhea.

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