HPLC/PDA/ESI-MS Evaluation of Saffron (Crocus sativus L.) Adulteration

The present study evaluated the reliability of the ISO/TS 3632-2 UV-Vis spectrometric method for saffron classification, making experiments on saffron samples to which were added increasing concentrations of common saffron spice adulterants (safflower, marigold and turmeric). The results showed that the ISO/TS 3632-2 method is not able to detect addition of up to 10-20%, w/w, of saffron adulterants. For additions from 20 to 50%, w/w, of the three adulterants, saffron was classified in a wrong category; addition of higher than 50%, w/w, determined variations in the investigated parameters that did not allow identification of the product as “saffron”. In all cases, the method did not permit the recognition of the nature of the adulterant. On the contrary, the specificity of the HPLC/PDA/MS technique allowed the unequivocal identification of adulterant characteristic marker molecules that could be recognized by the values of absorbance and mass. The selection of characteristic ions of each marker molecule has revealed concentrations of up to 5%, w/w, for safflower and marigold and up to 2% for turmeric. In addition, the high dyeing power of turmeric allowed the determination of 2%, w/w, addition using exclusively the HPLC/PDA technique.

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Qualitative Profile and Quantitative Determination of Flavonoids from Crocus Sativus L. Petals by LC-MS/MS

Natural Product Communications ◽

10.1177/1934578x0800301215 ◽

2008 ◽

Vol 3 (12) ◽

pp. 1934578X0800301 ◽

Cited By ~ 4

Author(s):

Paola Montoro ◽

Carlo I. G. Tuberoso ◽

Mariateresa Maldini ◽

Paolo Cabras ◽

Cosimo Pizza

Keyword(s):

Quantitative Determination ◽

Methanolic Extract ◽

Waste Product ◽

Crocus Sativus ◽

Esi Ms ◽

Quantitative Studies ◽

Derivatives Of ◽

Crocus Sativus L

From the methanolic extract of Crocus sativus petals nine known flavonoids have been isolated and identified, including glycosidic derivatives of quercetin and kaempferol as major compounds (1–2), and their methoxylated and acetylated derivatives. Additionally, LC-ESI-MS qualitative and LC-ESI-MS/MS quantitative studies of the major compounds of the methanolic extract were performed. The high content of glycosylated flavonoids could give value to C. sativus petals, which are a waste product in the production of the spice saffron.

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Determination of some phenolic compounds in Crocus sativus L. corms and its antioxidant activities study

Pharmacognosy Magazine ◽

10.4103/0973-1296.75906 ◽

2011 ◽

Vol 7 (25) ◽

pp. 74 ◽

Cited By ~ 27

Author(s):

K Abdi ◽

S Safarian ◽

N Esmaeili ◽

H Ebrahimzadeh

Keyword(s):

Phenolic Compounds ◽

Antioxidant Activities ◽

Crocus Sativus ◽

Crocus Sativus L

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In‐depth analysis of crocetin ester glycosides from dried/processed stigmas of Crocus sativus L. by HPLC‐ESI‐MS n ( n = 2, 3)

Phytochemical Analysis ◽

10.1002/pca.2817 ◽

2019 ◽

Vol 30 (3) ◽

pp. 346-356 ◽

Cited By ~ 1

Author(s):

Ernst Pittenauer ◽

Edita Rados ◽

Anthony Tsarbopoulos ◽

Günter Allmaier

Keyword(s):

Crocus Sativus ◽

Esi Ms ◽

Depth Analysis ◽

Crocus Sativus L

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Application of AFLP-Method in Plant Sample Identification

Acta Agraria Debreceniensis ◽

10.34101/actaagrar/10/3497 ◽

2003 ◽

pp. 207-213

Author(s):

Ákos Zubor ◽

Gyula Surányi ◽

József Prokisch ◽

Zoltán Győri ◽

György Borbély

Keyword(s):

Dna Polymorphism ◽

Fragment Length Polymorphism ◽

Crocus Sativus ◽

Plant Sample ◽

Science Literature ◽

Natural Hybrid ◽

The World ◽

Biological Methods ◽

Crocus Sativus L

One possible method for the determination of DNA-polymorphism is the PCR-based AFLP (Amplified Fragment Length Polymorphism). This method had been succesfully introduced to the Department of Botany at University of Debrecen in 2000-2001 with the examination of hay saffron (Crocus sativus L.) and its allies. Hay saffron is grown as a spice for some thousand years producing the most expensive spice in the world. This plant is sterile, triploid reproduces only vegetatively with no fertile seeds. However its origin is unknown it exists only in cultivation and it is a mutated variety of another species or an artificial or natural hybrid. Usual methods for the systematic examination are restricted hence it seemed to be reasonable to apply molecular biological methods in its case. Results of this work include the introduction and many fold application of the method beside ensuring the consequences of science literature with determining the C. cartwrightianus to give the most similar genetical pattern to C. sativus.

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Investigation of the Effect of Disinfectants on Biofilm Forming Non-Fermenting Bacteria

UNIVERSITY NEWS. NORTH-CAUCASIAN REGION. NATURAL SCIENCES SERIES ◽

10.18522/1026-2237-2020-1-89-94 ◽

2020 ◽

pp. 89-94

Author(s):

Anna V. Aleshukina ◽

Elena V. Goloshva ◽

Tatyana I. Tverdokhlebova

Keyword(s):

Biofilm Formation ◽

Economic Effect ◽

Mass Spectrometric ◽

Spectrometric Method ◽

Mass Spectrometric Method ◽

Fermenting Bacteria ◽

Sublethal Concentrations ◽

Selection Of ◽

Denaturation Of Proteins

The relevance of this study is determined by the wide prevalence in medical practice of biofilm infections caused, in particular, non-fermenting gram-negative bacteria with increased resistance to antibiotics and disinfectants. The aim of this study was to study the effect of disinfectants on biofilm formation of non-fermenting bacteria and to assess the possibility of using a mass spectrometric method to optimize the selection of disinfectants that affect the process of biofilm formation in non-fermenting bacteria. Results and conclusions. The drugs of choice for the reliable elimination of non-fermenting bacteria was hydro-gen peroxide and Ultradon, which microbially effect close to 100 %. The used solutions of disinfectants showed the same effect on the ability of non-fermenting bacteria to biofilm formation: the use of sublethal concentrations of these disinfectants led to a synchronous decrease in biofilm formation. The advantage of the mass spectrometric method of studying the sensitivity of non-fermenting bacteria to disinfectants is its objectivity, speed and ease of execution. The method gives an economic effect on time, labor and consumables compared to the traditionally used method of serial dilutions and the determination of viable clones of bacteria. Allows to investigate simultaneously sensitivity of cul-tures to different disinfectants on an assessment of denaturation of proteins with the subsequent change of mass spectrometric profiles.

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