scholarly journals Combined Analysis of the Root Bark Oil of Cleistopholis glauca by Chromatographic and Spectroscopic Techniques

2013 ◽  
Vol 8 (12) ◽  
pp. 1934578X1300801 ◽  
Author(s):  
Zana A. Ouattara ◽  
Jean Brice Boti ◽  
Coffy Antoine Ahibo ◽  
Félix Tomi ◽  
Joseph Casanova ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Silica Gel ◽  
Ivory Coast ◽  
Retention Indices ◽  
Spectroscopic Techniques ◽  
Root Bark ◽  
Oxygenated Compounds ◽  
Germacrene D ◽  
Main Components ◽  
C Nmr

The composition of root bark oil from Cleistopholis glauca Pierre ex Engler & Diels growing wild in Ivory Coast was investigated by GC (in combination with retention indices) and 13C NMR spectroscopy after partition of hydrocarbons and oxygenated compounds on silica gel. Thirty-one compounds have been identified. C. glauca produces a sesquiterpene-rich oil, patchoulenone (33.5%), cyperene (9.5%) and germacrene D (6.6%) being the main components. Special attention was paid to the identification and quantification of germacrene C (a heat-sensitive compound) and δ-elemene, which were achieved by a combination of GC(FID) and 13C NMR spectroscopy. The composition of C. glauca root bark and leaf oils differed drastically.

scholarly journals Composition and Variability of Leaf Oil from Monodora Crispata -13C NMR Identification of Heat-sensitive Compounds

2017 ◽  
Vol 12 (3) ◽  
pp. 1934578X1701200
Author(s):  
Tra Marc Gabin Djié Bi ◽  
Acafou Thierry Yapi ◽  
Zana Adama Ouattara ◽  
Janat Akhanovna Mamyrbekova-Békro ◽  
Mathieu Paoli ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Two Samples ◽  
Sesquiterpene Hydrocarbons ◽  
Germacrene D ◽  
Fourth Sample ◽  
Leaf Oil ◽  
Nmr Techniques ◽  
Main Components ◽  
Nmr Identification ◽  
C Nmr

The composition of four samples of essential oil isolated from the leaves of individual Monodora crispata trees growing wild in an Ivorian forest (Adiopodoumé) were investigated by a combination of chromatographic (GC(RI)) and spectrometric (GC-MS, 13C NMR) techniques. In total, fifty-seven compounds accounting for 92.3–98.7% of the whole composition were identified. These oils were characterized by the preeminence of sesquiterpene hydrocarbons and the content of the main components varied substantially from sample to sample. Two samples were largely dominated by germacrene D (67.3% and 76.3%, respectively), a third sample was represented by germacrene D (28.3%), germacrene C (14.3%), δ-elemene (12.1%) and β-elemene (9.3%) and the fourth sample was characterized by 3-dimethylallylindole (15.1%), germacrene D (12.0%) and cyperene (7.8%). 13C NMR spectroscopy was particularly efficient for the identification of heat-sensitive compounds.

scholarly journals Composition and Chemical Variability of the Cone Oil from Algerian Tetraclinis articulata

2016 ◽  
Vol 11 (8) ◽  
pp. 1934578X1601100 ◽  
Author(s):  
Maghnia Boussaïd ◽  
Chahrazed Bekhechi ◽  
Fewzia Atik-Bekkara ◽  
Mathieu Paoli ◽  
Joseph Casanova ◽  
...  
Keyword(s):  
Essential Oil ◽  
Retention Indices ◽  
Spectroscopic Techniques ◽  
Medicinal And Aromatic Plants ◽  
Tetraclinis Articulata ◽  
Chemical Variability ◽  
Western Algeria ◽  
Main Components ◽  
First Time ◽  
C Nmr

Very little is known about the yield and chemical composition of the essential oil isolated from cones of Tetraclinis articulata (Vahl) Masters (Cupressaceae). In continuation of our on-going work devoted to the valorisation of medicinal and aromatic plants from Tlemcen Province (North-Western Algeria), cones have been harvested in eight locations (coastal township and highlands) and 40 oil samples have been isolated by hydrodistillation. Six oil samples were analyzed by a combination of chromatographic [GC(Retention indices)] and spectroscopic techniques (GC-MS, 13C NMR). Then, the remaining 34 oil samples were analyzed by [GC(RI)] and 13C NMR and the 40 compositions were submitted to statistical analysis. Essential oil yields varied substantially from sample to sample (0.17 to 1.13%, w/w). The cone oils consisted mainly of monoterpenes, with α-pinene (47.1-73.6%) and limonene (6.5-21.9%) being the main components. (E)-β-Caryophyllene and germacrene D were the major sesquiterpenes. Diterpenes, ( Z) and ( E)-biformene were identified for the first time in T. articulata essential oil. PCA analysis of the data allowed the distinction of two groups, the second group being sub-divided into two sub-groups. Groups and sub-groups were differentiated with respect to their contents of α-pinene and limonene and, to a lesser extent, of sesquiterpenes.

scholarly journals Integrated Analysis by GC(RI), GC-MS and 13C NMR of Fortunella Japonica Leaf Volatiles Obtained by Hydrodistillation, Microwave- assisted Hydrodistillation and Hydrolate Extraction

2017 ◽  
Vol 12 (3) ◽  
pp. 1934578X1701200
Author(s):  
Sylvain Sutour ◽  
François Luro ◽  
Joseph Casanova ◽  
Félix Tomi
Keyword(s):  
Essential Oil ◽  
Tertiary Alcohol ◽  
Appreciable Amount ◽  
Integrated Analysis ◽  
Spectroscopic Techniques ◽  
Leaf Volatiles ◽  
Microwave Assisted ◽  
Germacrene D ◽  
Main Components ◽  
C Nmr

The chemical composition of the essential oil (EO), microwave extract (ME) and hydrolate extract (HE) from the same batch of leaves of Fortunella japonica, was investigated by combination of chromatographic (GC, CC) and spectroscopic techniques (GC-MS, 13C NMR). F. japonica essential oil and extracts are complex mixtures of 28–60 compounds being mainly oxygenated sesquiterpenes. The EO composition was dominated by germacrene D (14.9%), β-elemol (9.1%), cis-guai-6-en-10p-ol (6.3%), β-eudesmol (5.5%), and δ-elemene (5.2%). Limonene was the unique monoterpene identified at appreciable amount (7.1%). The extract obtained by microwave assisted hydrodistillation contained as main components: β-elemol (12.4%), germacrene D (9.9%), cis-guai-6-en-10β-ol (9.0%), β-eudesmol (8.2%), germacra-1(10),5-dien-4α-ol (7.1%) and α-eudesmol (6.4%). Finally, the highest content of oxygenated sesquiterpenes (near 92%) was found in the hydrolate extract displaying cryptomeridiol (23.3%, but totally absent in the EO and ME), β-eudesmol (20.6%) and α-eudesmol (10.7%). Combined analysis by chromatographic and spectroscopic techniques appeared useful for identification of various sesquiterpenols bearing a tertiary alcohol function.

scholarly journals Essential Oil of Three Uvaria species from Ivory Coast

2011 ◽  
Vol 6 (11) ◽  
pp. 1934578X1100601
Author(s):  
Koffi A. Muriel ◽  
Tonzibo Z. Félix ◽  
Gilles Figueredo ◽  
Pierre Chalard ◽  
Yao T. N'guessan
Keyword(s):  
Essential Oil ◽  
Ivory Coast ◽  
Stem Bark ◽  
Root Bark ◽  
Benzyl Benzoate ◽  
Germacrene D ◽  
Leaf Oil ◽  
Main Components ◽  
Different Parts ◽  
Type Apparatus

Different parts of Uvaria ovata (Dunals) A, U. anonoides Baker f. and U. tortilis A. Chev were collected from Ivory Coast, in Toumodi (center), Agboville (south-east) and Sikensi (south), respectively. The essential oils, obtained by hydrodistillation using a Clevenger-type apparatus, were investigated by CG and CG/MS. The proportion of the chromatographed constituents identified varied from 92.5% to 98.5%. For U. ovata, the root bark oil comprised mainly camphene (10.2%), β-pinene (10.1%), epi–α-cadinol (13.2%) and intermedeol (9.7%), while the oil of the stem bark was dominated by epi–α-cadinol (27.3%), intermedeol (11.9%) and benzyl benzoate (13.4%). The oil of the leaves showed β-caryophyllene (15.6%), germacrene D (24.2%) and benzyl benzoate (18.3%) as the most abundant constituents. The leaf oil of U. anonoides was rich in 2,5-dimethoxy- p-cymene (15.5%), bicyclogermacrene (21.3%) and benzyl benzoate (8.7%), while, γ-terpinene (31.7%), β–caryophyllene (23.9%) and germacrene D (15.8%) constituted the main components of the stem bark oil of U. tortilis.

scholarly journals Composition and Chemical Variability of Ivoirian Xylopia staudtii Leaf Oil

2015 ◽  
Vol 10 (6) ◽  
pp. 1934578X1501000
Author(s):  
Thierry Acafou Yapi ◽  
Jean Brice Boti ◽  
Antoine Coffy Ahibo ◽  
Sylvain Sutour ◽  
Ange Bighelli ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Nmr Spectroscopy ◽  
Essential Oils ◽  
Oil Composition ◽  
Chemical Variability ◽  
Germacrene D ◽  
Leaf Oil ◽  
Nmr Techniques ◽  
C Nmr

The chemical composition of a leaf oil sample from Ivoirian Xylopia staudtii Engler & Diels (Annonaceae) has been investigated by a combination of chromatographic [GC(RI)] and spectroscopic (GC-MS, 13C NMR) techniques. Thirty-five components that accounted for 91.8% of the whole composition have been identified. The oil composition was dominated by the furanoguaiadienes furanoguaia-1,4-diene (39.0%) and furanoguaia-1,3-diene (7.5%), and by germacrene D (17.5%). The composition of twelve other leaf oil samples demonstrated qualitative homogeneity, but quantitative variability. Indeed, the contents of the major components varied substantially: furanoguaia-1,4-diene (24.7–51.7%) and germacrene D (5.9–24.8%). The composition of X. staudtii leaf oil is close to that of X. rubescens leaf oil but varied drastically from those of the essential oils isolated from other Xylopia species. 13C NMR spectroscopy appeared as a powerful and complementary tool for analysis of sesquiterpene-rich essential oils.

scholarly journals Chemical Composition of the Essential Oils from Two Vietnamese Asarum Species: A. glabrum and A. cordifolium

2013 ◽  
Vol 8 (2) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Tran Huy Thai ◽  
Ophélie Bazzali ◽  
Tran Minh Hoi ◽  
Nguyen Anh Tuan ◽  
Félix Tomi ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Nmr Spectroscopy ◽  
Endangered Species ◽  
Essential Oil ◽  
Essential Oils ◽  
Spectroscopic Techniques ◽  
Red Data Book ◽  
Data Book ◽  
First Time ◽  
C Nmr

The essential oil isolated from Asarum cordifolium C. E. C. Fischer recently discovered in Vietnam, and A. glabrum Merr., an endangered species listed as vulnerable in the Red Data Book of Vietnam, have been analyzed by a combination of chromatographic and spectroscopic techniques including 13C NMR spectroscopy. The composition of A. cordifolium essential oil, investigated for the first time, was dominated by elemicin (82.5%). The essential oil isolated from A. glabrum contained safrole (41.9%) as its major component and was characterized by the diversity of phenylpropanoids contained in this oil (10 compounds).

scholarly journals Chemical Composition and Variability of Leaf and Berry oils from Corsican Juniperus macrocarpa

2018 ◽  
Vol 13 (2) ◽  
pp. 1934578X1801300
Author(s):  
Joséphine Ottavioli ◽  
Ange Bighelli ◽  
Joseph Casanova ◽  
Félix Tomi
Keyword(s):  
Principal Component Analysis ◽  
Statistical Analysis ◽  
Chemical Composition ◽  
Principal Component ◽  
Component Analysis ◽  
Germacrene D ◽  
Leaf Oil ◽  
Main Components ◽  
Main Component ◽  
C Nmr

The chemical composition of five leaf oil samples and eighteen berry oil samples from Corsican Juniperus macrocarpa have been investigated by GC(RI), GC-MS and 13C NMR. The composition of berry oils was dominated by monoterpene hydrocarbons with α-pinene (56.4-78.9%) as main component followed by myrcene (2.2-11.9%). Germacrene D (4.5-103%) was the major sesquiterpene. The contents of the main components of leaf oils varied drastically from sample to sample: α-pinene (28.7-76.4%), δ3-carene (up to 17.3%), β-phellandrene (up to 12.3%), manoyl oxide (up to 8.1%). The occurrence of the unusual ( Z)-pentadec-6-en-2-one (0.1-1.2%) should be pointed out. Statistical analysis (Principal Component Analysis and k- means partition) suggested a unique group with atypical samples.

scholarly journals Composition and Chemical Variability of Root Bark oil from Ivoirian Cleistopholis patens

2018 ◽  
Vol 13 (6) ◽  
pp. 1934578X1801300
Author(s):  
Zana A. Ouattara ◽  
Thierry A. Yapi ◽  
Yves-Alain Békro ◽  
Akhanovna J. Mamyrbékova-Békro ◽  
Mathieu Paoli ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Juvenile Hormone ◽  
Retention Indices ◽  
Bornyl Acetate ◽  
Chemical Compositions ◽  
Root Bark ◽  
Juvenile Hormone Iii ◽  
Nmr Data ◽  
Chemical Variability ◽  
C Nmr

The chemical composition of root bark oil of Cleistopholis patens (Benth.) Engl. & Diels from Côte d'Ivoire was determined by GC (in combination with retention indices), GC-MS and 13C NMR. The contents of the major compounds varied drastically from sample to sample: patchoulenone (0–70.5%), β-pinene (0–51.9%), bornyl acetate (0.5–31.2%), α-pinene (0.2–25.7%), juvenile hormone III (0.3–22.2%) and β-elemol (0–18.8%). Three chemical compositions may be differentiated, dominated by i) patchoulenone, ii) β-pinene and α-pinene, iii) juvenile hormone III, accompanied by bornyl acetate or β-elemol. 13C NMR data of patchoulenol (3-patchoulen-5- exo-ol) are reported.

Analysis of Insecticidal Azadirachta indica A. Juss. Fractions

2004 ◽  
Vol 59 (1-2) ◽  
pp. 104-112 ◽  
Author(s):  
Bina Shaheen Siddiqui ◽  
Munawwer Rasheed ◽  
Firdous Ilyas ◽  
Tahsin Gulzar ◽  
Rajput Mohammad Tariq ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Azadirachta Indica ◽  
Anopheles Stephensi ◽  
Ethanolic Extract ◽  
Retention Indices ◽  
Spectroscopic Techniques ◽  
Methyl Pentadecanoate ◽  
Ethyl Hexadecanoate ◽  
First Time ◽  
C Nmr

As a result of chemical investigation on the ethanolic extract of fresh fruit coatings of Azadirachta indica A. Juss. (neem), twenty-seven compounds were identified in non-polar to less polar fractions which showed pesticidal activity determined by WHO method against Anopheles stephensi Liston. These identifications were basically made through GC-EIMS and were further supported by other spectroscopic techniques, including 13C NMR, UV and FTIR as well as retention indices. Thus sixteen n-alkanes, 1-16; three aromatics 2,6-bis-(1,1- dimethylethyl)-4-methyl phenol (17), 2-(phenylmethylene)-octanal (20), 1,2,4-trimethoxy-5- (1Z-propenyl)-benzene (27); three benzopyranoids 3,4-dihydro-4,4,5,8-tetramethylcoumarin (18), 3,4-dihydro-4,4,7,8-tetramethylcoumarin-6-ol (19), 1,3,4,6,7,8-hexahydro-4,6,6,7,8,8-hexamethyl- cyclopenta[g]-2-benzopyran (22); one sesquiterpene methyl-3,7,11-trimethyl-2E,- 6E,10-dodecatrienoate (21); three esters of fatty acids methyl 14-methyl-pentadecanoate (23), ethyl hexadecanoate (24), ethyl 9Z-octadecenoate (25) and one monoterpene 3,7-dimethyl- 1-octen-7-ol (26) were identified. Except 6, 8, 24 and 25 all these compounds were identified for the first time from the pericarp and fifteen of these, 1-3, 7, 9, 10, 17-23, 26, 27, are hitherto unreported previously from any part of the tree. Although this tree is a rich source of various natural products, it is the first report of identification of mono- and sesquiterpenes 26 and 21 and a potent antioxidant, 17.

scholarly journals New Natural Oxygenated Sesquiterpenes and Chemical Composition of Leaf Essential Oil from Ivoirian Isolona dewevrei (De Wild. & T. Durand) Engl. & Diels

Molecules ◽  
2020 ◽  
Vol 25 (23) ◽  
pp. 5613
Author(s):  
Didjour Albert Kambiré ◽  
Jean Brice Boti ◽  
Thierry Acafou Yapi ◽  
Zana Adama Ouattara ◽  
Ange Bighelli ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
13C Nmr ◽  
2D Nmr ◽  
Integrated Analysis ◽  
Spectroscopic Techniques ◽  
Germacrene D ◽  
Leaf Essential Oil ◽  
Main Components ◽  
First Time

This study aimed to investigate the chemical composition of the leaf essential oil from Ivoirian Isolona dewevrei. A combination of chromatographic and spectroscopic techniques (GC(RI), GC-MS and 13C-NMR) was used to analyze two oil samples (S1 and S2). Detailed analysis by repetitive column chromatography (CC) of essential oil sample S2 was performed, leading to the isolation of four compounds. Their structures were elucidated by QTOF-MS, 1D and 2D-NMR as (10βH)-1β,8β-oxido-cadin-4-ene (38), 4-methylene-(7αH)-germacra-1(10),5-dien-8β-ol (cis-germacrene D-8-ol) (52), 4-methylene-(7αH)-germacra-1(10),5-dien-8α-ol (trans-germacrene D-8-ol) (53) and cadina-1(10),4-dien-8β-ol (56). Compounds 38, 52 and 53 are new, whereas NMR data of 56 are reported for the first time. Lastly, 57 constituents accounting for 95.5% (S1) and 97.1% (S2) of the whole compositions were identified. Samples S1 and S2 were dominated by germacrene D (23.6 and 20.5%, respectively), followed by germacrene D-8-one (8.9 and 8.7%), (10βH)-1β,8β-oxido-cadin-4-ene (7.3 and 8.7), 4-methylene-(7αH)-germacra-1(10),5-dien-8β-ol (7.8 and 7.4%) and cadina-1(10),4-dien-8β-ol (7.6 and 7.2%). Leaves from I. dewevrei produced sesquiterpene-rich essential oil with an original chemical composition, involving various compounds reported for the first time among the main components. Integrated analysis by GC(RI), GC-MS and 13C-NMR appeared fruitful for the knowledge of such a complex essential oil.

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