Antiproliferative Diterpenes from a Malleastrum sp. from the Madagascar dry forest [1]

An ethanol extract of leaves of the plant species Malleastrum sp. collected in northern Madagascar afforded the new clerodane diterpene 18-oxo-cleroda-3,13-dien-16,15-olide (1), together with the three known clerodane diterpenes 16,18-dihydroxykolavenic acid lactone (2), solidagolactone (3) and (-)-kolavenol (4), and the known labdane diterpene 3-oxo-ent-Iabda-8(17),13-dien-15,16-olide (5). Compounds 1, 3, and 4 showed moderate antiproliferative activities against the A2780 ovarian cancer cell line, with the IC50 values of 3.01 ± 0.8, 7.84 ± 0.2, and 17.9 ± 3 μM, respectively. The structure elucidations of all compounds were carried out based on analysis of NMR and mass spectroscopic data. The relative stereochemistry of compound 1 was determined by NOESY NMR spectrum.

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Two Antiproliferative Saponins of Tarenna grevei from the Madagascar Dry Forest [1]

Natural Product Communications ◽

10.1177/1934578x1200700604 ◽

2012 ◽

Vol 7 (6) ◽

pp. 1934578X1200700 ◽

Cited By ~ 3

Author(s):

Liva Harinantenaina ◽

Peggy J. Brodie ◽

Martin W. Callmander ◽

L. Jérémie Razafitsalama ◽

Vincent E. Rasamison ◽

...

Keyword(s):

Ovarian Cancer Cell ◽

Ovarian Cancer Cell Line ◽

Cancer Cell Line ◽

Ethanol Extract ◽

2D Nmr ◽

Dry Forest ◽

Triterpene Saponins ◽

2D Nmr Spectroscopy ◽

Bioassay Guided Fractionation ◽

Chemical Evidence

Antiproliferative bioassay-guided fractionation of the ethanol extract of the endemic Malagasy Rubiaceous plant Tarenna grevei led to the isolation of two new antiproliferative oxygenated oleanane triterpene saponins. The structures of the two active compounds were elucidated as 23-hydroxylongispinogenin 3- O-β-D-glucopyranoside (1) and longispinogenin 3- O-β-D-glucopyranosyl (1→2)-β-D-glucopyranoside (3) by analyses of their spectral data including 1D- and 2D-NMR spectroscopy and chemical evidence. Compounds 1 and 3 displayed moderate antiproliferative activity against the A2780 ovarian cancer cell line with IC50 values of 7.6 and 4 μM, respectively.

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A New Bioactive Diterpene Glycoside from Molinaea Retusa from the Madagascar Dry Forest

Natural Product Communications ◽

10.1177/1934578x1300800903 ◽

2013 ◽

Vol 8 (9) ◽

pp. 1934578X1300800 ◽

Cited By ~ 1

Author(s):

Alexander L. Eaton ◽

Liva Harinantenaina ◽

Peggy J. Brodie ◽

Maria B. Cassera ◽

Jessica D. Bowman ◽

...

Keyword(s):

Ovarian Cancer ◽

Plasmodium Falciparum ◽

Ovarian Cancer Cell ◽

Ovarian Cancer Cell Line ◽

Cancer Cell Line ◽

Ethanol Extract ◽

Dry Forest ◽

Human Ovarian Cancer ◽

Spectroscopic Methods ◽

Human Ovarian Cancer Cell

In a continuing collaboration in a search for new antiproliferative compounds in Madagascar as part of an International Cooperative Biodiversity Group (ICBG), an ethanol extract of Molinaea retusa Radlk. (Sapindaceae) was investigated on the basis of its moderate antiproliferative activity against the A2780 human ovarian cancer cell line (IC50 16 μg/mL). One new compound, 2″,3″,4″,6′-de- O-acetylcupacinoside (1, IC50 15.4 μM) and two known compounds, cupacinoside (2, IC50 9.5 μM) and 6-de- O-acetylcupacinoside (3, IC50 10.9 μM), were isolated by bioassay-directed fractionation using liquid-liquid partitioning, column chromatography, and HPLC. Compounds 2 and 3 also had moderate antiplasmodial activities, with IC50 values of 4.0 and 6.4 μM, respectively, against Plasmodium falciparum, Dd2 strain. The structures were determined using spectroscopic methods.

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DIFFERENTIAL EXPRESSION OF MTOR COMPONENTS IN ENDOMETRIOSIS AND ENDOMETRIOID OVARIAN CANCER CELL LINE: EFFECTS OF RAPAMYCIN, RESVERATROL AND BEZ-235

10.26226/morressier.597eedbbd462b80296ca1bb1 ◽

2017 ◽

Author(s):

Chatterjee Jayanta

Keyword(s):

Ovarian Cancer ◽

Cell Line ◽

Differential Expression ◽

Cancer Cell ◽

Ovarian Cancer Cell ◽

Ovarian Cancer Cell Line ◽

Cancer Cell Line ◽

Endometrioid Ovarian Cancer

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Extracellular Matrix Proteins Expression Profiling in Chemoresistant Variants of the A2780 Ovarian Cancer Cell Line

BioMed Research International ◽

10.1155/2014/365867 ◽

2014 ◽

Vol 2014 ◽

pp. 1-9 ◽

Cited By ~ 38

Author(s):

Radosław Januchowski ◽

Piotr Zawierucha ◽

Marcin Ruciński ◽

Michał Nowicki ◽

Maciej Zabel

Keyword(s):

Ovarian Cancer ◽

Extracellular Matrix ◽

Drug Resistance ◽

Cell Line ◽

Cancer Cell ◽

Ovarian Cancer Cell ◽

Ovarian Cancer Cell Line ◽

Cancer Cell Line ◽

Altered Expression ◽

Expression Levels

Ovarian cancer is the leading cause of death among gynaecological malignancies. Extracellular matrix (ECM) can affect drug resistance by preventing the penetration of the drug into cancer cells and increased resistance to apoptosis. This study demonstrates alterations in the expression levels of ECM components and related genes in cisplatin-, doxorubicin-, topotecan-, and paclitaxel-resistant variants of the A2780 ovarian cancer cell line. Affymetrix Gene Chip Human Genome Array Strips were used for hybridisations. The genes that had altered expression levels in drug-resistant sublines were selected and filtered by scatter plots. The genes that were up- or downregulated more than fivefold were selected and listed. Among the investigated genes, 28 genes were upregulated, 10 genes were downregulated, and two genes were down- or upregulated depending on the cell line. Between upregulated genes 12 were upregulated very significantly—over 20-fold. These genes included COL1A2, COL12A1, COL21A1, LOX, TGFBI, LAMB1, EFEMP1, GPC3, SDC2, MGP, MMP3, and TIMP3. Four genes were very significantly downregulated: COL11A1, LAMA2, GPC6, and LUM. The expression profiles of investigated genes provide a preliminary insight into the relationship between drug resistance and the expression of ECM components. Identifying correlations between investigated genes and drug resistance will require further analysis.

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