scholarly journals Phenolic Compounds from the Fruits of Medemia argun, a Food and Medicinal Plant of Ancient Egypt

2016 ◽  
Vol 11 (3) ◽  
pp. 1934578X1601100 ◽  
Author(s):  
Milena Masullo ◽  
Arafa I. Hamed ◽  
Usama A. Mahalel ◽  
Cosimo Pizza ◽  
Sonia Piacente
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Ancient Egypt ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Phenolic Fraction ◽  
Oxidative Reactions ◽  
Phytochemical Investigation ◽  
Trolox Equivalent ◽  
In Vitro Antioxidant Activity

Medemia argun is a mysterious and little known monotypic fan palm from the Nubian Desert Oases of southern Egypt and northern Sudan. Its fruits have been found in the tombs from the 5th Dynasty (ca. 2500 BC) to Roman times (6–7th century AD), including the celebrated tomb of Tutankhamun. In ancient Egypt, the fruits of this palm were widely distributed and were highly valued, as confirmed by their frequent occurrence in offerings in the tombs. İn order to elucidate the chemical composition of the phenolic fraction, phytochemical investigation of the BuOH extract of fruits was carried out to afford eight compounds (1–8), among which was the new 2,4-dihydroxy-6-methylacetophenone 2- O-β-D-glucopyranoside (1). With the aim to investigate if the high shelf life of M. argun fruits could be related to the occurrence of antioxidant principles that were able to prevent oxidative reactions, the evaluation was carried out of the in vitro antioxidant activity by Trolox equivalent antioxidant capacity (TEAC) assay of the extract and isolated compounds.

scholarly journals Evaluation of theIn VitroandIn VivoAntioxidant Potentials ofSudarshanaPowder

2018 ◽  
Vol 2018 ◽  
pp. 1-5 ◽  
Author(s):  
Weerakoon Achchige Selvi Saroja Weerakoon ◽  
Pathirage Kamal Perera ◽  
Dulani Gunasekera ◽  
Thusharie Sugandhika Suresh
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Thiobarbituric Acid ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Control Group ◽  
Thiobarbituric Acid Reactive Substance ◽  
Trolox Equivalent ◽  
Antioxidant Effects

Sudarshanapowder (SP) is one of the most effective Ayurveda powder preparations for paediatric febrile conditions. The objective of the present study was to evaluate thein vitroandin vivoantioxidant potentials of SP. Thein vitroantioxidant effects were evaluated using ABTS radical cation decolourization assay where the TROLOX equivalent antioxidant capacity (TEAC) was determined. Thein vivoantioxidant activity of SP was determined in Wistar rats using the Lipid Peroxidation (LPO) assay in serum. Thein vitroassay was referred to as the TROLOX equivalent antioxidant capacity (TEAC) assay. For thein vivoassay, animals were dosed for 21 consecutive days and blood was drawn to evaluate the MDA level. Thein vitroantioxidant activity of 0.5 μg of SP was equivalent to 14.45 μg of standard TROLOX. The percentage inhibition against the radical formation was50.93±0.53%. The SP showed a statistically significant (p<0.01) decrease in the serum level of thiobarbituric acid-reactive substance in the test rats when compared with the control group. These findings suggest that the SP possesses potent antioxidant activity which may be responsible for some of its reported bioactivities.

scholarly journals Quantification of the Antioxidant Activity of Plant Extracts: Analysis of Sensitivity and Hierarchization Based on the Method Used

Antioxidants ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 76 ◽  
Author(s):  
Natividad Chaves ◽  
Antonio Santiago ◽  
Juan Carlos Alías
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Plant Species ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Antioxidant Compounds ◽  
Frap Assay ◽  
Trolox Equivalent

Plants have a large number of bioactive compounds with high antioxidant activity. Studies for the determination of the antioxidant activity of different plant species could contribute to revealing the value of these species as a source of new antioxidant compounds. There is a large variety of in vitro methods to quantify antioxidant activity, and it is important to select the proper method to determine which species have the highest antioxidant activity. The aim of this work was to verify whether different methods show the same sensitivity and/or capacity to discriminate the antioxidant activity of the extract of different plant species. To that end, we selected 12 species with different content of phenolic compounds. Their extracts were analyzed using the following methods: 2,2-di-phenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity assay, ferric reducing (FRAP) assay, Trolox equivalent antioxidant capacity (ABTS) assay, and reducing power (RP) assay. The four methods selected could quantify the antioxidant capacity of the 12 study species, although there were differences between them. The antioxidant activity values quantified through DPPH and RP were higher than the ones obtained by ABTS and FRAP, and these values varied among species. Thus, the hierarchization or categorization of these species was different depending on the method used. Another difference established between these methods was the sensitivity obtained with each of them. A cluster revealed that RP established the largest number of groups at the shortest distance from the root. Therefore, as it showed the best discrimination of differences and/or similarities between species, RP is considered in this study as the one with the highest sensitivity among the four studied methods. On the other hand, ABTS showed the lowest sensitivity. These results show the importance of selecting the proper antioxidant activity quantification method for establishing a ranking of species based on this parameter.

Peppermint and Basil Essential Oils: Chemical Composition, in vitro Antioxidant Activity and in vivo Estimation of Skin Irritation

2019 ◽  
Vol 22 (4) ◽  
pp. 979-993
Author(s):  
Ljiljana P. Stanojevic ◽  
Jelena S. Stanojevic ◽  
Vesna Lj. Savic ◽  
Dragan J. Cvetkovic ◽  
Ana Kolarevic ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Skin Irritation ◽  
In Vitro Antioxidant Activity

scholarly journals Antioxidant Activity and Chemical Composition of Essential Oil from Atriplex Undulata

2010 ◽  
Vol 5 (11) ◽  
pp. 1934578X1000501 ◽  
Author(s):  
Silvana A. Rodriguez ◽  
Ana P. Murray
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Dpph Radical ◽  
Active Compounds ◽  
Aerial Parts ◽  
Stems And Leaves ◽  
In Vitro Antioxidant Activity

The essential oil from aerial parts (stems and leaves) of Atriplex undulata (Moq) D. Dietr. (Chenopodiaceae) has been studied for its in vitro antioxidant activity. The chemical composition of the oil obtained by hydrodistillation was determined by GC and GC-MS. The major constituents were p-acetanisole (28.1%), β-damascenone (9.3%), β-ionone (5.1%), viridiflorene (4.7%) and 3-oxo-α-ionol (2.2%). The antioxidant activity of the oil was determined by two methods: Crocin bleaching inhibition (Krel= 0.72 ± 0.15) and scavenging of the DPPH radical (IC50 = 36.2 ± 1.6 μg/mL). The presence of active compounds like p-acetanisole, carvone, vanillin, 4-vinylguaiacol, guaiacol, terpinen-4-ol and α- terpineol could explain the antioxidant activity observed for this oil.

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