scholarly journals New Alkaloids and Anti-inflammatory Constituents from the Leaves of Antidesma ghaesembilla

2017 ◽  
Vol 12 (1) ◽  
pp. 1934578X1701200
Author(s):  
Phan Van Kiem ◽  
Le Canh Viet Cuong ◽  
Do Thi Trang ◽  
Nguyen Xuan Nhiem ◽  
Hoang Le Tuan Anh ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Inhibitory Activity ◽  
No Production ◽  
Raw 264.7 ◽  
Chemical Methods ◽  
Raw 264.7 Macrophages ◽  
Chemical Structures ◽  
Bv2 Cells ◽  
Inhibitory Activities ◽  
Physical And Chemical

Using various chromatographic methods, two new alkaloids, antidesoic acids A (1) and B (2) along with fourteen known compounds (3-16) were isolated from the leaves of Antidesma ghaesembilla Gaertn. Their chemical structures were elucidated by physical and chemical methods. All the isolated compounds were evaluated for their inhibitory activity on LPS-stimulated nitric oxide (NO) production in BV2 cells and RAW 264.7 macrophages. Bisflavone 8 significantly inhibited LPS-stimulated NO production in BV2 cells and RAW 264.7 macrophages with IC50 values of 5.4 and 8.0 μM, respectively. Compounds 1–3, 7, 10, 12, 14, and 16 showed moderate inhibitory activities with IC50 values ranging from 11.7 to 77.4 μM.

scholarly journals Inhibition of nitric oxide production in lipopolysaccharide-activated RAW 264.7 macrophages by Jeju plant extracts

2009 ◽  
Vol 2 (4) ◽  
pp. 245-249 ◽  
Author(s):  
Eun-Jin Yang ◽  
Eun-Young Yim ◽  
Gwanpil Song ◽  
Gi-Ok Kim ◽  
Chang-Gu Hyun
Keyword(s):  
Nitric Oxide ◽  
Plant Extracts ◽  
Inhibitory Activity ◽  
Inflammatory Diseases ◽  
No Production ◽  
Raw 264.7 ◽  
Nitric Oxide Production ◽  
Raw 264.7 Macrophages ◽  
Oxide Production ◽  
Potent Inhibition

Inhibition of nitric oxide production in lipopolysaccharide-activated RAW 264.7 macrophages by Jeju plant extractsNitric oxide (NO) produced in large amounts by inducible nitric oxide synthase (iNOS) is known to be responsible for the vasodilation and hypotension observed during septic shock and inflammation. Thus, inhibitors of iNOS may be useful candidates for the treatment of inflammatory diseases accompanied by the overproduction of NO. In this study, we prepared alcoholic extracts of Jeju plants and screened them for their inhibitory activity against NO production in lipopolysaccharide (LPS)-activated macrophages. Among the 260 kinds of plant extract tested, 122 extracts showed potent inhibitory activity towards NO production by more than 25% at a concentration of 100 μg/mL. Plants such asMalus sieboldii, Vaccinium oldhamii, Corylus hallaisanensis, Carpinus laxiflora, Styrax obassia, andSecurinega suffruticosashowed the most potent inhibition (above 70%) at a concentration of 100 μg/mL. The cytotoxic effects of the plant extracts were determined by colorimetric MTT assays and most plant extracts exhibited only moderate cytotoxicity at 100 μg/mL. Therefore, these plants should be considered promising candidates for the further purification of bioactive compounds and would be useful for the treatment of inflammatory diseases accompanying overproduction of NO.

IMMUNOMODULATORY EFFECTS OF FAR-INFRARED RAY IRRADIATION VIA INCREASING CALMODULIN AND NITRIC OXIDE PRODUCTION IN RAW 264.7 MACROPHAGES

2009 ◽  
Vol 21 (05) ◽  
pp. 317-323 ◽  
Author(s):  
Ting-Kai Leung ◽  
Yung-Sheng Lin ◽  
Yen-Chou Chen ◽  
Huey-Fang Shang ◽  
Yi-Hsuan Lee ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Scientific Evidence ◽  
Far Infrared ◽  
No Production ◽  
Raw 264.7 ◽  
Nitric Oxide Production ◽  
Immunomodulatory Effects ◽  
Raw 264.7 Macrophages ◽  
Immunomodulatory Properties ◽  
Far Infrared Ray

Far-infrared ray (FIR) radiation has been shown to be beneficial to human health; however, little scientific evidence of its mechanisms has been provided. In the present study, we investigated the effect of nonthermal-enhanced FIR on the expression of calmodulin (Cam) protein and nitric oxide (NO) production by RAW 264.7 macrophages. Results indicated a significant increase in Cam protein in FIR-treated RAW 264.7 macrophages with or without the addition of lipopolysaccharide (LPS). In addition, the amount of NO was slightly higher but increased significantly in FIR plus LPS-treated RAW 264.7 macrophages. Data of the present study provide the first evidence to indicate the immunomodulatory properties of FIR through increasing Cam protein and NO production in RAW 264.7 macrophages.

scholarly journals Bioactive Compounds from an Endophytic Fungi Nigrospora Aurantiaca

2021 ◽  
Author(s):  
Safwan Safwan ◽  
George Hsiao ◽  
Tzong-Huei Lee ◽  
Ching-Kuo Lee
Keyword(s):  
Bioactive Compounds ◽  
Endophytic Fungi ◽  
Inhibitory Activity ◽  
Spectroscopic Data ◽  
Biological Activities ◽  
Fungal Strain ◽  
No Production ◽  
Chemical Structures ◽  
Inhibitory Activities ◽  
Melaleuca Leucadendra

Abstract Background: Many groups of fungi live as an endophyte in plants . Endophytic fungi could produce the bioactive compounds which was new or know compounds. Various biological activities of bioactive compounds from endophytic fungi had been reported, including anti-inflammatory and anticancer. Chemical investigation and biological activities of bioactive compounds from endophytic fungi from Melaleuca leucadendra Linn. have not yet been reportedResults: One new compound, namely nigaurdiol (1), together with five known compounds, xyloketal K (2), bostrycin (3), deoxybostrycin (4), xylanthraquinone (5), and ergosterol (6), were isolated from the Melaleuca leucadendra Linn. associated fungal strain Nigrospora aurantiaca #TMU062. Their chemical structures were elucidated by spectroscopic data and compared with literatures. All isolated compounds were evaluated for NO production inhibitory activity in LPS-activated microglial BV-2 cells. Conclusions: Compound 6 exhibited markedly inhibitory activities on NO production with IC50 values of 7.2 ± 1.4 µM and the survival of the cells was 90.8 ± 6.7% at the concentration of 10 µM.

scholarly journals A New Phenylpropanoid from the Roots of Solanum melongena L. and Evaluation of Anti-inflammatory Activity

2021 ◽  
Vol 15 (4) ◽  
pp. 261-266
Author(s):  
Yan Liu ◽  
Xin Yin ◽  
Yanping Sun ◽  
Yuan Liu ◽  
Dongxv Lu ◽  
...  
Keyword(s):  
Solanum Melongena ◽  
Ethanol Extract ◽  
2D Nmr ◽  
No Production ◽  
Raw 264.7 ◽  
Chemical Structures ◽  
Raw 264.7 Cell ◽  
Ic50 Values ◽  
Inhibitory Activities ◽  
Raw 264.7 Cell Line

Fifteen phenylpropanoids were isolated from the ethanol extract of the roots of Solanum melongena L., including a new compound, melongenapanoid A (1), together with fourteen known compounds (2-15). Their chemical structures were elucidated by 1D and 2D NMR and HR-MS data according to those values of the literatures. The fourteen known compounds (2-15) were all firstly isolated from this plant. While, the isolates were evaluated for the inhibitory activities on nitric oxide (NO) production induced by lipopolysaccharide (LPS) in RAW 264.7 cell line. Compounds 2, 4 and 5 showed moderate inhibition of NO production with IC50 values of 28.7, 24.4 and 32.6 μM, respectively.

scholarly journals Synthesis of New Triarylpyrazole Derivatives Possessing Terminal Sulfonamide Moiety and Their Inhibitory Effects on PGE2 and Nitric Oxide Productions in Lipopolysaccharide-Induced RAW 264.7 Macrophages

Molecules ◽  
2018 ◽  
Vol 23 (10) ◽  
pp. 2556 ◽  
Author(s):  
Mohammed S. Abdel-Maksoud ◽  
Mohammed I. El-Gamal ◽  
Mahmoud M. Gamal El-Din ◽  
Yunji Choi ◽  
Jungseung Choi ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Pyrazole Ring ◽  
Inhibitory Effect ◽  
No Production ◽  
Raw 264.7 ◽  
Design Synthesis ◽  
Raw 264.7 Macrophages ◽  
New Compounds ◽  
Oxide Synthase

This article describes the design, synthesis, and in vitro anti-inflammatory screening of new triarylpyrazole derivatives. A total of 34 new compounds were synthesized containing a terminal arylsulfonamide moiety and a different linker between the sulfonamide and pyridine ring at position 4 of the pyrazole ring. All the target compounds were tested for both cytotoxicity and nitric oxide (NO) production inhibition in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages. Compounds 1b, 1d, 1g, 2a, and 2c showed the highest NO inhibition percentages and the lowest cytotoxic effect. The most potent derivatives were tested for their ability to inhibit prostaglandin E2 (PGE2) in LPS-induced RAW 264.7 macrophages. The IC50 for nitric oxide inhibition, PGE2 inhibition, and cell viability were determined. In addition, 1b, 1d, 1g, 2a, and 2c were tested for their inhibitory effect on LPS-induced inducible nitric oxide synthase (iNOS) and Cyclooxygenase 2 (COX-2) protein expression as well as iNOS enzymatic activity.

scholarly journals Identification of Bioactive Natural Product from the Stems and Stem Barks of Cornus walteri: Benzyl Salicylate Shows Potential Anti-Inflammatory Activity in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages

Pharmaceutics ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 443
Author(s):  
Dahae Lee ◽  
Akida Alishir ◽  
Tae Su Jang ◽  
Ki Hyun Kim
Keyword(s):  
Nitric Oxide ◽  
Chemical Constituents ◽  
Stem Bark ◽  
Inflammatory Activity ◽  
No Production ◽  
Raw 264.7 ◽  
Ongoing Research ◽  
Raw 264.7 Macrophages ◽  
Iκb Kinase ◽  
Anti Inflammatory

Cornus walteri (Cornaceae), known as Walter’s dogwood, has been used to treat dermatologic inflammation and diarrheal disease in traditional oriental medicine. As part of an ongoing research project to discover natural products with biological activities, the anti-inflammatory potential of compounds from C. walteri in lipopolysaccharide (LPS)-stimulated mouse RAW 264.7 macrophages were explored. Phytochemical analysis of the methanol extract of the stem and stem bark of C. walteri led to the isolation of 15 chemical constituents. These compounds were evaluated for their inhibitory effects on the production of the proinflammatory mediator nitric oxide (NO) in LPS-stimulated macrophages, as measured by NO assays. The molecular mechanisms underlying the anti-inflammatory activity were investigated using western blotting. Our results demonstrated that among 15 chemical constituents, lupeol and benzyl salicylate inhibited NO production in LPS-activated RAW 264.7 macrophages. Benzyl salicylate was more efficient than NG-monomethyl-L-arginine mono-acetate salt (L-NMMA) in terms of its inhibitory effect. In addition, the mechanism of action of benzyl salicylate consisted of the inhibition of phosphorylation of IκB kinase alpha (IKKα), IκB kinase beta (IKKβ), inhibitor of kappa B alpha (IκBα), and nuclear factor kappa B (NF-κB) in LPS-stimulated macrophages. Furthermore, benzyl salicylate inhibited the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). Taken together, these results suggest that benzyl salicylate present in the stem and stem bark of C. walteri has potential anti-inflammatory activity, supporting the potential application of this compound in the treatment of inflammatory diseases.

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