scholarly journals Chemical Composition and Antimicrobial Activity of the Essential Oil From Euphorbia helioscopia L.

2020 ◽  
Vol 15 (9) ◽  
pp. 1934578X2095324
Author(s):  
Qing Zhu ◽  
Mei-Lin Jiang ◽  
Feng Shao ◽  
Guang-Qiang Ma ◽  
Qiang Shi ◽  
...  

The chemical composition and antimicrobial activity of the essential oil from the aerial parts of Euphorbia helioscopia L. were investigated by gas chromatography-mass spectrometry (GC-MS), GC, and microdilution methods. Thirty-five compounds, representing 83.51% of the total oil, were identified. 1,6-Dihydrocarveol (31.39%), carvone (16.79%), menthol (8.23%), and trans-dihydrocarvone (5.53%) were the principal constituents of the oil. The essential oil exhibited strong antimicrobial activity against strains of the bacteria, Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Shigella dysenteriae, and a strain of the fungus Candida albicans with a minimal inhibitory concentration value of 31.25 μg/mL and minimum bactericidal concentration values of 31.25 μg/mL ( S. aureus), 62.50 μg/mL ( E. faecalis), >62.50 μg/mL ( E. coli), >62.50 μg/mL ( S. dysenteriae), and >62.50 μg/mL ( C. albicans). These results indicated that the essential oil from the aerial parts of E. helioscopia could be used to control diseases caused by these microbes.

2015 ◽  
Vol 43 (2) ◽  
pp. 432-438 ◽  
Author(s):  
Aneta WESOŁOWSKA ◽  
Monika GRZESZCZUK ◽  
Dorota JADCZAK ◽  
Paweł NAWROTEK ◽  
Magdalena STRUK

The chemical composition of the essential oils obtained by hydrodistillation from the aerial parts of Thymus serpyllum and Thymus serpyllum‘Aureus’ has been investigated by gas chromatography-mass spectrometry (GC-MS). Forty-seven compounds (99.67% of the total oil) wereidentified in the essential oil of T. serpyllum. The main components found in the oil were carvacrol (37.49%), -terpinene (10.79%), -caryophyllene (6.51%), p-cymene (6.06%), (E)--ocimene (4.63%) and -bisabolene (4.51%). Similarly, carvacrol (44.93%), -terpinene(10.08%), p-cymene (7.39%) and -caryophyllene (6.77%) dominated in the oil of T. serpyllum ‘Aureus’. A total of forty three compounds wereidentified in this oil, representing 99.49% of the total oil content. On the basis of the obtained data it was proved that the content of 1-octen-3-ol,eucalyptol, (Z)--ocimene, (E)--ocimene, -terpinene, carvacrol methyl ether, germacrene D and -bisabolene was significantly higher for T.serpyllum while T. serpyllum ‘Aureus’ was characterized by a significantly higher content of 3-octanone, 3-octanol, p-cymene, borneol andcarvacrol. The isolated essential oils were evaluated for their antimicrobial activity against nine reference strains (Escherichia coli, Staphylococcusaureus, Staphylococcus epidermidis, Streptococcus agalactiae, Enterococcus faecalis, Bacillus cereus, Micrococcus luteus, Proteus vulgaris and Candidaalbicans) by the microdilution technique. Based on this test, the minimum inhibitory concentrations (MIC) of essential oil were calculated. Thevolatile oil obtained from T. serpyllum showed the highest antimicrobial activity relative to the strain of E. coli (MIC=0.025 μL/mL) and to theyeast C. albicans (MIC=0.05 μL/mL). Similarly, a significant antimicrobial activity exhibited T. serpyllum ‘Aureus’ essential oil, although the MICvalues obtained in that case for E. coli and C. albicans strains were twice as high and were respectively 0.05 μL/mL and 0.1 μL/mL.


2014 ◽  
Vol 9 (2) ◽  
pp. 1934578X1400900
Author(s):  
Rajesh K. Joshi

The essential oil obtained from the aerial parts of Croton bonplandianus Baill. was analyzed by gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). A total of 37 compounds have been identified, representing 96.2% of the total oil. The main constituents were identified as β-caryophyllene (16.7%), germacrene D (14.7%), borneol (8.3%), Z-β-damascenone (6.(%), isobornyl acetate (6.2%), α-humulene (6.1%), germacrene A (5.2%) and caryophyllene oxide (4.5%). The oil was rich in sesquiterpene hydrocarbons (60.1%).


2018 ◽  
Vol 19 (45) ◽  
pp. 38-43 ◽  
Author(s):  
Uugangerel Erdenetsogt ◽  
Choijamts Gotov ◽  
Kerstin Voigt ◽  
Stefan Bartram ◽  
Wilhelm Boland ◽  
...  

The chemical composition and antimicrobial activity of the essential oil from the aerial parts of Pyrethrum pulchrum Ledeb. were investigated. Dried plant material was hydro-distillated yielding 0.1% of essential oil. The oil was analyzed by GC-MS techniques. Fifty-five compounds were identified representing 99.7% of the total oil composition. Camphor was the predominant compound (33.9%) followed by linalool (21.1%) and α-pinene (9.0%). The antimicrobial activity of the oil was determined using the disk diffusion method against Gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus and Enterococcus faecalis), Gram-negative bacteria (Pseudomonas aeruginosa and Escherichia coli), Mycobacterium vaccae and fungi (Candida albicans, Sporidiobolus salmonicolor and Penicillum notatum). The essential oil of P. pulchrum displays an intermediate activity against selected bacteria.


1970 ◽  
Vol 46 (2) ◽  
pp. 191-194 ◽  
Author(s):  
AK Sarker ◽  
JU Chowdhury ◽  
HR Bhuiyan

Essential oil obtained from hydrodistillation of Adhatoda vasica (Nees.) leaves was analyzed by gas chromatograph-mass spectrometer (GC-MS). Hydrodistillation of the Adhatoda vasica (Nees.) leaves yielded 0.096% (v/w) of essential oil. Eleven compounds comprising about 100% of the total oil were identified. The most abundant components of essential oil were 1,2,3, trimethyl benzene (1.51 %), borneol (58.60 %), ethanonaphthalene (2.82 %), 1,1,4a trimethyl-5,6-dimethylenedecahydro naphthalene (5.28 %), 2,tert-butyl-1,4- dimethoxy benzene (6.50 %), bicyclo[jundec-4-ene,4,11-trimethyl-8-methylene (14.56 %), hexa- methyl dewar benzene (0.87 %), alphacaryophyllene (1.95 %), cycloproplejazulene (1.48 %), caryophyllene oxide (2.35 %) and 2-naphthalenemethanol (1.46 %). The microbial activity of the oil was screened against Bacillus subtilis, Salmonella typhimurium, Staphylococcus aureus, and E. coli. It was found that all mentioned microorganisms were more or less sensitive to this essential oil. Key Words: Adhatoda vasica; Essential oil; Chemical composition; Antimicrobial activity. DOI: http://dx.doi.org/10.3329/bjsir.v46i2.8185 Bangladesh J. Sci. Ind. Res. 46(2), 191-194, 2011  


Author(s):  
Messaoud Ramdani ◽  
RIMA HAICHOUR1 ◽  
TAKIA LOGRADA ◽  
PIERRE CHALARD ◽  
GILLES FIGUEREDO4

Abstract. Haichour R, Lograda T, Ramdani M, Chalard P, Figueredo G. 2020. Chemical composition and antimicrobial activity of Pinus halepensis from Algeria. Biodiversitas 21: 4345-4360. The chemical composition of Pinus halepensis essential oils and their antibacterial activities were investigated. Plant samples were collected in the flowering stage from the East locality of Algeria. The aerial parts of P. halepensis obtained from fifteen localities were subjected to a hydro-distillation, providing pale yellow viscous oils. The obtained yield average was 0.64 ± 0.37%. The analysis of the chemical composition of essential oils was performed by using GC and GC / MS. A total of thirty-seven compounds representing 99.4 ± 0.5% of the total oils were identified in P. halepensis. The hydrocarbon compounds were found to dominate essential oils in P. halepensis. In addition, the major compounds were caryophyllene-E (29.06 ± 8.5%), myrcene (19.14 ± 6.67%), a-pinene (16.86 ± 3.35%), phenyl ethyl 3-methyl butanoate (5.67 ± 2.47%), a-humulene (4.81 ± 1.43%), terpinolene (3.94 ± 1.6%) and sabinene (3.11 ± 1.84%). Based on their chemical composition, essential oil samples were clustered in five groups by UPGMA analysis. The first group includes the Tafrent essential oil population. It is characterized by the phenyl ethyl-3-methyl butanoate – Sabinene ‒ Δ3-carene chemotype. The second group was subdivided into two clusters. The first cluster or chemotype was characterized by the myrcene ‒ α-pinene ‒ caryophyllene-E. The second chemotype was characterized by caryophyllene-E ‒ α-humulene ‒ myrcene. The essential oil of P. halepensis is an effective antimicrobial against Staphylococcus aureus; Klebsiella pneumonia; Salmonella enteric spp arizonae; Listeria innocua; Proteus mirabilis and Bacillus cereus, and no effect on the E. coli and Pseudomonas aeruginosa.


2012 ◽  
Vol 524-527 ◽  
pp. 2269-2272 ◽  
Author(s):  
Ke Hai Liu ◽  
Qing Zhu ◽  
Jing Jing Zhang ◽  
Jian Feng Xu ◽  
Xi Chang Wang

The chemical composition of the essential oil of Mentha spicata from China was investigated by the Gas chromatography/mass spectrometry (GC/MS) analysis. 21 compounds were identified, representing 97.10% of the total oil. The major compounds in the essential oil were carvone (65.33%), limonene (18.19%), dihydrocarvone (2.97%) and camphene (2.34%). The oil showed antioxidant activity assessed using DPPH assay. The antimicrobial activity was investigated in order to evaluate its efficacy against 5 microorganisms using MIC and MBC methods. The results indicated that spearmint oil showed inhibitory and bactericidal effect on all test microorganisms. It was highly effective against E. coli, S. cerivisiae and P. citrinum. S. cerivisiae was more susceptible to spearmint oil (MIC 0.78125 uL/mL and MBC 6.25 uL/mL). The cytotoxicity of spearmint oil was evaluated on Hela cells using the MTT assay. The oil showed quite strong cytotoxicity on the cell lines with an IC50of about 2.08ug/mL.


2009 ◽  
Vol 4 (7) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Rajesh K. Joshi ◽  
Chitra Pande ◽  
Mohammad H. K. Mujawar ◽  
Sanjiva D. Kholkute

The essential oil composition of the aerial parts of Anaphalis nubigena DC. var. monocephala (DC.) C. B. Clarke collected from Pindari glacier at a height of 3300 m, was analyzed by using GC and GC/MS. Sixty components were identified, accounting for 95.9% of the total oil. The main constituents were α-guaiene (12.3%), γ-muurolene (10.4%), γ-cadinene (8.3%), α-muurolol (7.4%), α-gurjunene (6.0%) and α-bulnesene (5.8%). The oil was found to be rich in sesquiterpene hydrocarbons (60.1%). The oil was active against Escherichia coli (NCIM 2065) and Klebsiella pneumoniae (NCIM 2957), with MIC values of 125 μg/mL and 500 μg/mL, respectively.


2020 ◽  
Vol 42 (3) ◽  
Author(s):  
Hoang Thi Binh ◽  
Tran Thi Bao Tram ◽  
Do Ngoc Dai ◽  
Vuong Thuy Tien ◽  
Le Minh Tam ◽  
...  

In the present study, chemical composition and antibacterial properties of essential oil obtained from the aerial parts of the Melicope pteleifolia (Champ. ex Benth.) T.G Hartley in Dalat were evaluated. Essential oil was isolated through hydro-distillation. Twenty-nine constituents comprising 100% of the essential oil were characterized by gas chromatography/mass spectrometry (GC-MS) techniques. The major compounds in the essential oil were (+)-Sabinene (34.73%), Cis-α-bergamotene (13.15%), Z-α-trans-bergamotol (5.28%), β-mycrene (4.98%), and 1,3,6-octatriene, 3,7-dimethyl-(4.71%). Antibacterial activities of Melicope pteleifolia essential oil were investigated against Gram-positive and-negative bacteria. Results showed significant activities against Streptococcus pyogenes and Escherichia coli using an agar well diffusion method. The application of this essential oil in preventing and eliminating bacteria could be useful in fields as medicine and cosmetics. 


2014 ◽  
Vol 9 (7) ◽  
pp. 1934578X1400900
Author(s):  
Rajesh K. Joshi

The chemical composition of the hydro-distilled essential oil obtained from the flowering aerial parts of Vernonia albicans DC. (Asteraceae) was analyzed by gas chromatography equipped with a flame ionization detector (GC-FID) and gas chromatography coupled with a mass spectrometry (GC/MS). Thirty-nine compounds have been identified, representing 97.5% of the total oil. The major constituents were β-caryophyllene (34.3%), γ-amorphene (19.5%), 9-epi-β-caryophyllene (6.9%), and α-pinene (6.9%). The oil was found to be rich in sesquiterpene hydrocarbons (73.9%).


2007 ◽  
Vol 2 (5) ◽  
pp. 1934578X0700200
Author(s):  
Fadwa El Hanbali ◽  
Ahmed El Hakmaoui ◽  
Fouad Mellouki ◽  
Lahoussine El Rhaffari ◽  
Mohamed Akssira

The essential oil of the aerial parts of Anvillea radiata Coss. & Dur. was examined by GC-MS. Twenty-nine constituents were identified, representing 88.8% of the total. 6-Oxocyclonerolidol (66.6%) and 6-hydroxycyclonerolidol (11.4%) were found to be the major components. The disc diffusion method, used for antimicrobial activity testing, showed that the essential oil had a significant antibacterial activity against all tested microorganisms.


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