scholarly journals An electron stain for elastic fibers using orcein.

1977 ◽  
Vol 25 (4) ◽  
pp. 306-308 ◽  
Author(s):  
H Nakamura ◽  
C Kanai ◽  
V Mizuhira
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Uranyl Acetate ◽  
Elastic Fibers ◽  
Lead Citrate ◽  
Thin Sections ◽  
Electron Microscopes ◽  
Orcein Staining

Orcein was found to be useful as an electron-opaque stain for elastic fibers in epoxy-sections. Ultra-thin sections of aorta were treated with elastica stain containing 0.1-0.3% orcein and counterstained in uranyl acetate and lead citrate. Elastic fibers were densely and specifically demonstrated in the stroma and near smooth muscle cells. The result of orcein staining has a comparable appearance under both light and electron microscopes.

Immunogold localization of HMB-45 antigen in pulmonary lymphangiomyomatosis

1995 ◽  
Vol 53 ◽  
pp. 998-999
Author(s):  
J.M. Minda ◽  
E. Dessy ◽  
G. G. Pietra
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Room Temperature ◽  
Osmium Tetroxide ◽  
Muscle Cells ◽  
Ultrastructural Localization ◽  
Reproductive Age ◽  
Thin Sections ◽  
Smooth Muscle Proliferation ◽  
Hmb 45

Pulmonary lymphangiomyomatosis (PLAM) is a rare disease occurring exclusively in women of reproductive age. It involves the lungs, lymph nodes and lymphatic ducts. In the lungs, it is characterized by the proliferation of smooth muscle cells around lymphatics in the bronchovascular bundles, lobular septa and pleura The nature of smooth muscle proliferation in PLAM is still unclear. Recently, reactivity of the smooth muscle cells for HMB-45, a melanoma-related antigen has been reported by immunohistochemistry. The purpose of this study was the ultrastructural localization of HMB-45 immunoreactivity in these cells using gold-labeled antibodies.Lung tissue from three cases of PLAM, referred to our Institution for lung transplantation, was embedded in either Poly/Bed 812 post-fixed in 1% osmium tetroxide, or in LR White, without osmication. For the immunogold technique, thin sections were placed on Nickel grids and incubated with affinity purified, monoclonal anti-melanoma antibody HMB-45 (1:1) (Enzo Diag. Co) overnight at 4°C. After extensive washing with PBS, grids were treated with Goat-anti-mouse-IgG-Gold (5nm) (1:10) (Amersham Life Sci) for 1 hour, at room temperature.

scholarly journals ULTRASTRUCTURAL INTERRELATIONSHIPS OF NERVE PROCESSES AND SMOOTH MUSCLE CELLS IN THREE DIMENSIONS

1966 ◽  
Vol 28 (1) ◽  
pp. 37-49 ◽  
Author(s):  
J. C. Thaemert
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Osmium Tetroxide ◽  
Three Dimensional ◽  
Muscle Cells ◽  
Uranyl Acetate ◽  
Intestinal Wall ◽  
Three Dimensions ◽  
Serial Sections ◽  
Muscularis Externa

The muscularis externa of the intestinal wall of frogs was fixed in osmium tetroxide, embedded in Vestopal-W, serially sectioned for electron microscopy, and stained with uranyl acetate. A method to obtain individually mounted and properly positioned serial sections is described. The three-dimensional techniques used during the course of this investigation demonstrate that it is possible to examine carefully relatively large areas of tissue on individual serial sections with the electron microscope and subsequently to construct montages of electron micrographs of pertinent areas from each section. Several carefully rendered interrelationships of nerve processes and smooth muscle cells in three dimensions are exhibited and described. Recent studies of other neuro-effector relationships are discussed in relation to the present status of the nature and organization of the autonomic nervous system in visceral organs.

scholarly journals Proteoglycans in primate arteries. I. Ultrastructural localization and distribution in the intima.

1975 ◽  
Vol 67 (3) ◽  
pp. 660-674 ◽  
Author(s):  
T N Wight ◽  
R Ross
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Ultrastructural Localization ◽  
Ruthenium Red ◽  
Basement Membranes ◽  
Macaca Nemestrina ◽  
Elastic Fibers ◽  
Surface Coat ◽  
The Matrix

Proteoglycans were identified and localized histochemically and ultrastructurally in normal and hyperplastic arterial intimas in nonhuman primates (Macaca nemestrina). These regions were consistently more alcianophilic than the adjacent medial layers and this alcianophilia was absent after treatment with glycosaminoglycan-degradative enzymes. Ultrastructurally, the intimal intercellular matrix consisted of numerous, irregularly shaped, 200-500-A diameter granules possessing 30--60-A diameter filamentous projections, and these granules were dispersed between collagen and elastic fibers. The granules exhibited a marked affinity for ruthenium red and were interconnected via their filamentous projections. The ruthenium red-positive granules were intimately associated with the plasma membrane of intimal smooth muscle cells and attached to collagen fibrils and elastic fibers. The matrix granules were completely removed after testicular hyaluronidase or chondroitinase ABC digestion but only partially removed after leech hyaluronidase treatment. These results suggest that the matrix granules contain some hyaluronic acid and one or more isomers of chondroitin sulfate. In addition to the large ruthenium red-positive matrix granules, a smaller class of ruthenium red-positive granule (100--200-A diameter) was present within the basement membranes beneath the endothelium and surrounding the smooth muscle cells. Ruthenium red also exhibited an affinity for the surface coat of the smooth muscle cells. The potential importance of proteoglycans in arterial intimal hyperplasia is discussed.

scholarly journals P715Regulation of elastic fibers and smooth muscle cells in the mechanism of fibrosis found in patients with initial and intense varicose lesions: a clinical correlation

2014 ◽  
Vol 103 (suppl 1) ◽  
pp. S130.3-S131 ◽  
Author(s):  
J Regadera ◽  
JP Velasco-Martin ◽  
G Espana-Caparros ◽  
LM Reparaz ◽  
LA Condezo-Hoyos ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Elastic Fibers ◽  
Clinical Correlation

ORGANIZATION AND RELATIVE CONTENT OF SMOOTH MUSCLE CELLS, COLLAGEN AND ELASTIC FIBERS IN THE CORPUS CAVERNOSUM OF RAT PENIS

2000 ◽  
Vol 164 (5) ◽  
pp. 1802-1806 ◽  
Author(s):  
ANA C.A.D. PINHEIRO ◽  
WALDEMAR S. COSTA ◽  
LUIS E.M. CARDOSO ◽  
FRANCISCO J.B. SAMPAIO
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Corpus Cavernosum ◽  
Muscle Cells ◽  
Relative Content ◽  
Elastic Fibers

scholarly journals Extracellular Vesicles Derived from Primary Adipose Stromal Cells Induce Elastin and Collagen Deposition by Smooth Muscle Cells within 3D Fibrin Gel Culture

2021 ◽  
Vol 8 (5) ◽  
pp. 51
Author(s):  
Eoghan M. Cunnane ◽  
Aneesh K. Ramaswamy ◽  
Katherine L. Lorentz ◽  
David A. Vorp ◽  
Justin S. Weinbaum
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Stromal Cells ◽  
Biological Effects ◽  
Muscle Cells ◽  
Arterial Disease ◽  
Protein Quantification ◽  
Elastic Fibers ◽  
Positive Effects ◽  
Adipose Derived Stromal Cells

Macromolecular components of the vascular extracellular matrix (ECM), particularly elastic fibers and collagen fibers, are critical for the proper physiological function of arteries. When the unique biomechanical combination of these fibers is disrupted, or in the ultimate extreme where fibers are completely lost, arterial disease can emerge. Bioengineers in the realms of vascular tissue engineering and regenerative medicine must therefore ideally consider how to create tissue engineered vascular grafts containing the right balance of these fibers and how to develop regenerative treatments for situations such as an aneurysm where fibers have been lost. Previous work has demonstrated that the primary cells responsible for vascular ECM production during development, arterial smooth muscle cells (SMCs), can be induced to make new elastic fibers when exposed to secreted factors from adipose-derived stromal cells. To further dissect how this signal is transmitted, in this study, the factors were partitioned into extracellular vesicle (EV)-rich and EV-depleted fractions as well as unseparated controls. EVs were validated using electron microscopy, dynamic light scattering, and protein quantification before testing for biological effects on SMCs. In 2D culture, EVs promoted SMC proliferation and migration. After 30 days of 3D fibrin construct culture, EVs promoted SMC transcription of the elastic microfibril gene FBN1 as well as SMC deposition of insoluble elastin and collagen. Uniaxial biomechanical properties of strand fibrin constructs were no different after 30 days of EV treatment versus controls. In summary, it is apparent that some of the positive effects of adipose-derived stromal cells on SMC elastogenesis are mediated by EVs, indicating a potential use for these EVs in a regenerative therapy to restore the biomechanical function of vascular ECM in arterial disease.

Basement Membrane of Smooth Muscle Cell and Its Association With Elastic Tissue in Aorta

1967 ◽  
Vol 25 ◽  
pp. 36-37
Author(s):  
S.Y. Yu ◽  
C.N. Sun
Keyword(s):  
Smooth Muscle ◽  
Basement Membrane ◽  
Smooth Muscle Cells ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Muscle Cells ◽  
Uranyl Acetate ◽  
Elastic Tissue ◽  
Present Evidence ◽  
Elastic Unit

Organization and connection between smooth muscle cells and elastic tissues in aortas is not elucidated. Keech believed that the smooth muscle cells anchore on the elastic tissue. Among the fibers of the aortic connective tissue, there are collagen, elastic tissue and microfibril. This communication is to present evidence that the microfibrils which are derived from the basement membrane of the smooth muscle cells, play a role for connection between the smooth muscle cells and the elastic tissues.Thoracic aortas from new born rats were fixed with Dalton's chrom-osmium solution, embedded in Epon 812 and stained with uranyl acetate and lead citrate. Our previous work showed that the smooth muscle cell synthesized the elastic tissue during its differentiation prior to completing the process. At the actively synthesizing site of the cell, the basement membrane was replaced by numerous microfibrils (Fig. 1 MF). The microfibrils extended from the basement membrane toward the preformed elastic lamellae (EL), occasionally surrounded scallop-shaped elastic unit (EU).

A new adhesive protein mediating the interaction between mesenchymal cells and elastin fibers: Elastonectin

1988 ◽  
Vol 53 (1) ◽  
pp. 209-211
Author(s):  
Ladislas Robert ◽  
William Hornebeck
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Atherosclerotic Plaque ◽  
Muscle Cells ◽  
Mesenchymal Cells ◽  
Elastic Fibers ◽  
Elastic Tissue ◽  
Adhesive Protein

An inducible adhesive protein was demonstrated in smooth muscle cells and fibroblasts which mediate the adhesion of mesenchymal cells to elastic fibers. It is proposed to designate it elastonectin. This protein plays probably an important role in the morphogenesis of elastic tissue and its degradation is probably involved in the formation of the atherosclerotic plaque.

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