NO-tryptophan: a new small molecule located in the rat brain

<p>A highly specific monoclonal antibody directed against nitric oxide-tryptophan (NO-W) with good affinity (10<sup>-9 </sup>M) and specificity was developed. In the rat brain, using an indirect immunoperoxidase technique, cell bodies containing NO-W were exclusively found in the intermediate and dorsal parts of the lateral septal nucleus. No immunoreactive fibres were found in the rat brain. This work reports the first visualization and the morphological characteristics of cell bodies containing NO-W in the mammalian brain. The restricted distribution of NO-W in the rat brain suggests that this molecule could be involved in specific physiological mechanisms. </p>

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Immunocytochemical localization of tissue kallikrein in brain ventricular epithelium and hypothalamic cell bodies.

Journal of Histochemistry & Cytochemistry ◽

10.1177/33.9.3894505 ◽

1985 ◽

Vol 33 (9) ◽

pp. 951-953 ◽

Cited By ~ 33

Author(s):

J A Simson ◽

R Dom ◽

J Chao ◽

C Woodley ◽

L Chao ◽

...

Keyword(s):

Monoclonal Antibody ◽

Epithelial Cells ◽

Third Ventricle ◽

Immunoperoxidase Staining ◽

Tissue Kallikrein ◽

Immunocytochemical Localization ◽

Specific Monoclonal Antibody ◽

The Third ◽

Indirect Immunoperoxidase ◽

Rat Tissue

A specific monoclonal antibody against rat tissue kallikrein was used as the primary antibody for indirect immunoperoxidase staining of rat hypothalamus. Kallikrein was localized in the epithelial cells (ependyma) lining the third ventricle as well as in cell bodies of arcuate, supraoptic, paraventricular, and ventromedial nuclei.

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Characterization of a monoclonal antibody (SBU-1) made to the thymic rudiment of sheep.

Journal of Histochemistry & Cytochemistry ◽

10.1177/33.8.2410481 ◽

1985 ◽

Vol 33 (8) ◽

pp. 785-790 ◽

Cited By ~ 3

Author(s):

T R White ◽

C S Lee ◽

P French ◽

D Hewish ◽

M R Brandon

Keyword(s):

Monoclonal Antibody ◽

Epithelial Cells ◽

Immunoperoxidase Technique ◽

Fetal Sheep ◽

Wool Fibers ◽

Indirect Immunoperoxidase ◽

Low Sulfur ◽

Alpha Keratin ◽

Keratinized Epithelium

A monoclonal antibody (SBU-1) was raised to sheep thymic rudiment by fusion of NSI myeloma cells with spleen cells from BALB/c mice immunized with thymic rudiment isolated from fetal sheep between 25-30 days of gestation. By employing the indirect immunoperoxidase technique the antigen recognized by SBU-1 was found to be present in the epithelial reticular cells of the fetal sheep thymus. The intensity of staining decreased as gestation progressed. In the adult thymus the antigen was mainly restricted to Hassall's corpuscles and occasional epithelial cells in the medulla. In addition, the antigen was also shown to be present in epithelial cells of the small intestine, the bronchiole, the keratinized epithelium of the rumen, and the epithelial cells of the kidney tubules. By use of immunofluorescence the antigen was shown to be present in most of the cells of wool follicles and the cortex of developing wool fibers. Western blotting of SBU-1 against the low-sulfur alpha-keratin proteins of wool confirmed that the antigen recognized by SBU-1 belongs to a family of keratins. It was concluded that SBU-1 was raised against alpha-keratin expressed by the epithelial cells of the thymic rudiment and that the expression of this antigen on the reticular network of the thymus declined with advancement of pregnancy.

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