scholarly journals LN-6: a monoclonal antibody to vimentin expressed in non-hematopoietic mesenchymal cells and derived tumors and reactive in B5-fixed, paraffin-embedded tissues.

1989 ◽  
Vol 37 (9) ◽  
pp. 1363-1370 ◽  
Author(s):  
E Stathopoulos ◽  
G S Naeve ◽  
C R Taylor ◽  
A L Epstein
Keyword(s):  
Monoclonal Antibody ◽  
Human Cancer ◽  
Wide Spectrum ◽  
Mesenchymal Cells ◽  
Lymphoid Cells ◽  
Normal Cells ◽  
Immunohistochemical Diagnosis ◽  
Pathological Tissues

We generated a monoclonal antibody (MAb), designated LN-6, directed against human vimentin, which retains its immunoreactivity in B5-fixed, paraffin-embedded tissues. Like other anti-vimentin MAb, LN-6 was found to be reactive with a wide spectrum of human sarcomas and normal cells of mesenchymal derivation. However, unlike other similar reagents, LN-6 was unreactive with normal and malignant human lymphoid cells and therefore displays a more restricted immunoreactivity. Because of its ability to stain routinely processed pathological tissues and its marked reactivity with human sarcomas, LN-6 is a unique reagent for the immunohistochemical diagnosis of human cancer.

Characterization of C5aR expression on murine myeloid and lymphoid cells by the use of a novel monoclonal antibody

2003 ◽  
Vol 88 (1) ◽  
pp. 47-52 ◽  
Author(s):  
Afsaneh Soruri ◽  
Soyoung Kim ◽  
Ziba Kiafard ◽  
Jörg Zwirner
Keyword(s):  
Monoclonal Antibody ◽  
Lymphoid Cells ◽  
C5ar Expression

scholarly journals Over-Expression of βII-Tubulin and Especially Its Localization in Cell Nuclei Correlates with Poorer Outcomes in Colorectal Cancer

Cells ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 25 ◽  
Author(s):  
Kseniya Ruksha ◽  
Artur Mezheyeuski ◽  
Alexander Nerovnya ◽  
Tatyana Bich ◽  
Gennady Tur ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Monoclonal Antibody ◽  
Cancer Progression ◽  
Nuclear Localization ◽  
Cancer Growth ◽  
Transformed Cells ◽  
Regulatory Pathway ◽  
Cell Nuclei ◽  
Normal Cells ◽  
Over Expression

Tubulin is a heterodimer of α and β subunits, both existing as isotypes differing in amino acid sequence encoded by different genes. Specific isotypes of tubulin have associations with cancer that are not well understood. Previous studies found that βII-tubulin is expressed in a number of transformed cells and that this isotype is found in cell nuclei in non-microtubule form. The association of βII expression and its nuclear localization with cancer progression has not previously been addressed. We here used a monoclonal antibody to βII to examine patients with colorectal cancer and found that patients whose tumors over-express βII have a greatly decreased life expectancy which is even shorter in those patients with nuclear βII. Our results suggest that βII-tubulin may facilitate cancer growth and metastasis and, to accomplish this, may not need to be in microtubule form. Furthermore, βII expression and localization could be a useful prognostic marker. We also found that βII appears in the nuclei of otherwise normal cells adjacent to the tumor. It is possible therefore that cancer cells expressing βII influence nearby cells to do the same and to localize βII in their nuclei by an as yet uncharacterized regulatory pathway.

Parathyroiditis in the non-obese diabetic mouse – a new finding

1991 ◽  
Vol 131 (2) ◽  
pp. 193-NP ◽  
Author(s):  
J. Krug ◽  
A. J. K. Williams ◽  
P. E. Beales ◽  
I. Doniach ◽  
E. A. M. Gale ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Wide Spectrum ◽  
Lymphocytic Infiltration ◽  
Diabetic Mouse ◽  
Nod Mouse ◽  
Lymphocytic Thyroiditis ◽  
Thyroid Glands ◽  
Autoimmune Polyendocrinopathy ◽  
New Finding ◽  
Infiltrating Lymphocytes

ABSTRACT Autoimmune reactions to parathyroid cells have been observed in human autoimmune polyendocrinopathy, but such findings have not been described in animal models of polyendocrine autoimmunity. We report here three cases of lymphocytic infiltrations in 12 parathyroid glands identified in a total of 18 thyroid glands studied in the non-obese diabetic (NOD) mouse. The majority of parathyroid-infiltrating lymphocytes possessed the helper/inducer phenotype as defined by the L3T4 monoclonal antibody. Parathyroiditis was accompanied by lymphocytic thyroiditis only on one occasion, whereas in other cases of thyroiditis, lymphocytic infiltration of the parathyroid was undetectable. We conclude that parathyroiditis in the NOD mouse is part of the wide spectrum of autoimmunity observed in this animal model of diabetes. Journal of Endocrinology (1991) 131, 193–196

scholarly journals N-ω-chloroacetyl-l-ornithine, a new competitive inhibitor of ornithine decarboxylase, induces selective growth inhibition and cytotoxicity on human cancer cells versus normal cells

2014 ◽  
Vol 30 (3) ◽  
pp. 345-353 ◽  
Author(s):  
Miriam Marlene Medina-Enríquez ◽  
Verónica Alcántara-Farfán ◽  
Leopoldo Aguilar-Faisal ◽  
José Guadalupe Trujillo-Ferrara ◽  
Lorena Rodríguez-Páez ◽  
...  
Keyword(s):  
Growth Inhibition ◽  
Cancer Cells ◽  
Ornithine Decarboxylase ◽  
Human Cancer ◽  
Competitive Inhibitor ◽  
Selective Growth ◽  
Normal Cells ◽  
Human Cancer Cells

scholarly journals Bladder Cancer in the Genomic Era

2019 ◽  
Vol 143 (6) ◽  
pp. 695-704 ◽  
Author(s):  
Charles C. Guo ◽  
Bogdan Czerniak
Keyword(s):  
Bladder Cancer ◽  
Molecular Mechanisms ◽  
Complex Disease ◽  
Human Cancer ◽  
Wide Spectrum ◽  
Clinicopathologic Features ◽  
Pathologic Features ◽  
Genomic Characterization

Context.— Bladder cancer is a heterogeneous disease that exhibits a wide spectrum of clinical and pathologic features. The classification of bladder cancer has been traditionally based on morphologic assessment with the aid of immunohistochemistry. However, recent genomic studies have revealed that distinct alterations of DNA and RNA in bladder cancer may underlie its diverse clinicopathologic features, leading to a novel molecular classification of this common human cancer. Objective.— To update recent developments in genomic characterization of bladder cancer, which may shed insights on the molecular mechanisms underlying the origin of bladder cancer, dual-track oncogenic pathways, intrinsic molecular subtyping, and development of histologic variants. Data Sources.— Peer-reviewed literature retrieved from PubMed search and authors' own research. Conclusions.— Bladder cancer is likely to arise from different uroprogenitor cells through papillary/luminal and nonpapillary/basal tracks. The intrinsic molecular subtypes of bladder cancer referred to as luminal and basal exhibit distinct expression signatures, clinicopathologic features, and sensitivities to standard chemotherapy. Genomic characterization of bladder cancer provides new insights to understanding the biological nature of this complex disease, which may lead to more effective treatment.

scholarly journals Signal Transduction Mechanisms in Mesenchymal Cells

1994 ◽  
Vol 5 (3) ◽  
pp. 291-310 ◽  
Author(s):  
Bradley S. McAllister ◽  
John D. Walters ◽  
Merle S. Olson
Keyword(s):  
Signal Transduction ◽  
Phospholipase D ◽  
Tyrosine Kinases ◽  
Receptor Tyrosine Kinases ◽  
Wide Spectrum ◽  
Mesenchymal Cells ◽  
Signal Transduction Pathways ◽  
Regenerative Therapy ◽  
Initial Signal ◽  
Transduction Mechanisms

Mesenchymal cells are continually stimulated by a wide spectrum of biological mediators. These mediators bind to receptors on the cell surface and initiate a cascade of signaling events. The initial signal transduction pathways known to be stimulated in mesenchymal cells include phospholipase C, phospholipase D, phospholipase A2, adenylate cyclase, receptor tyrosine kinases, and receptor serine/threonine kinases. These pathways are reviewed and specific applications for therapeutic intervention in wound healing and regenerative therapy in the periodontium are discussed.

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