Parathyroiditis in the non-obese diabetic mouse – a new finding

1991 ◽  
Vol 131 (2) ◽  
pp. 193-NP ◽  
Author(s):  
J. Krug ◽  
A. J. K. Williams ◽  
P. E. Beales ◽  
I. Doniach ◽  
E. A. M. Gale ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Wide Spectrum ◽  
Lymphocytic Infiltration ◽  
Diabetic Mouse ◽  
Nod Mouse ◽  
Lymphocytic Thyroiditis ◽  
Thyroid Glands ◽  
Autoimmune Polyendocrinopathy ◽  
New Finding ◽  
Infiltrating Lymphocytes

ABSTRACT Autoimmune reactions to parathyroid cells have been observed in human autoimmune polyendocrinopathy, but such findings have not been described in animal models of polyendocrine autoimmunity. We report here three cases of lymphocytic infiltrations in 12 parathyroid glands identified in a total of 18 thyroid glands studied in the non-obese diabetic (NOD) mouse. The majority of parathyroid-infiltrating lymphocytes possessed the helper/inducer phenotype as defined by the L3T4 monoclonal antibody. Parathyroiditis was accompanied by lymphocytic thyroiditis only on one occasion, whereas in other cases of thyroiditis, lymphocytic infiltration of the parathyroid was undetectable. We conclude that parathyroiditis in the NOD mouse is part of the wide spectrum of autoimmunity observed in this animal model of diabetes. Journal of Endocrinology (1991) 131, 193–196

Presence and significance of NK cells in glioblastomas

1989 ◽  
Vol 70 (5) ◽  
pp. 728-731 ◽  
Author(s):  
Jesús Vaquero ◽  
Santiago Coca ◽  
Santiago Oya ◽  
Roberto Martínez ◽  
Josefa Ramiro ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Nk Cells ◽  
Survival Time ◽  
Tumor Cells ◽  
Lymphocytic Infiltration ◽  
Surface Marker ◽  
Content Type ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Staining ◽  
Fine Print

✓ A monoclonal antibody against the surface marker IOT-10 of natural killer (NK) cells was used to investigate the presence of these cells in a series of 25 glioblastomas. In 40% of the tumors, IOT-10-positive NK cells were found in small numbers scattered among the tumor cells. The presence of IOT-10-positive NK cells was not related to the degree of lymphocytic infiltration in the tumor as demonstrated by hematoxylin and eosin staining, nor did it appear to influence the survival time of the patients studied.

JUVENILE LYMPHOCYTIC THYROIDITIS

PEDIATRICS ◽  
1962 ◽  
Vol 30 (6) ◽  
pp. 917-926
Author(s):  
Krishna M. Saxena ◽  
John D. Crawford
Keyword(s):  
Antibody Test ◽  
Lymphocytic Infiltration ◽  
Thyroid Stimulating Hormone ◽  
Test Results ◽  
Lymphocytic Thyroiditis ◽  
Results Of Treatment ◽  
Two Factors ◽  
The Moment ◽  
Tsh Stimulation ◽  
Stimulation Of

Lymphocytic thyroiditis was the most common cause of nontoxic goiters in childhood, comprising about 40% of these and 20% of all goiters seen in our pediatric endocrine clinic. The diagnosis should be considered whenever a nontoxic goiter does not diminish significantly in size within about two weeks in response to thyroid-stimulating-hormone (TSH) suppressive doses of USP thyroid. Observations on 32 children with lymphocytic thyroiditis have been recorded. The diagnosis was proven histologically in 23. In nine patients the diagnosis was presumptive. The condition occurred mostly in preadolescent girls as a slowly developing firm, diffuse, and smooth or nubbly goiter with or without symptoms of anxiety, nervousness and pressure in the neck. The majority of patients were euthyroid when first encountered, though examples both of mild hyperthyroidism and hypothyroidism were also seen. A high protein-bound iodine value, a large discrepancy between protein-bound iodine and butanol-extractable iodine, and positive tanned erythrocyte antibody test results provided the best diagnostic criteria apart from biopsy. The pathological picture is one of hyperplasia with lymphocytic infiltration and atrophy of thyroid follicles and epithelium. Treatment consisted in giving TSH suppressive doses of thyroid for a prolonged period. The results of treatment were not entirely satisfactory. Genetic predisposition, probably manifested in an abnormal cellular hypersensitivity, and excessive TSH stimulation of the thyroid seem for the moment the two factors in pathogenesis best supported by laboratory evidence.

Ultrastructural and immunocytochemical pathology of polyglandular lymphocytic infiltration in the NOD mouse

1987 ◽  
Vol 3 ◽  
pp. S40-S40
Author(s):  
J MIYAGAWA ◽  
T HANAFUSA ◽  
A MIYAZAKI ◽  
H FUJINOKURIHARA ◽  
H NAKAJIMA ◽  
...  
Keyword(s):  
Lymphocytic Infiltration ◽  
Nod Mouse

scholarly journals LN-6: a monoclonal antibody to vimentin expressed in non-hematopoietic mesenchymal cells and derived tumors and reactive in B5-fixed, paraffin-embedded tissues.

1989 ◽  
Vol 37 (9) ◽  
pp. 1363-1370 ◽  
Author(s):  
E Stathopoulos ◽  
G S Naeve ◽  
C R Taylor ◽  
A L Epstein
Keyword(s):  
Monoclonal Antibody ◽  
Human Cancer ◽  
Wide Spectrum ◽  
Mesenchymal Cells ◽  
Lymphoid Cells ◽  
Normal Cells ◽  
Immunohistochemical Diagnosis ◽  
Pathological Tissues

We generated a monoclonal antibody (MAb), designated LN-6, directed against human vimentin, which retains its immunoreactivity in B5-fixed, paraffin-embedded tissues. Like other anti-vimentin MAb, LN-6 was found to be reactive with a wide spectrum of human sarcomas and normal cells of mesenchymal derivation. However, unlike other similar reagents, LN-6 was unreactive with normal and malignant human lymphoid cells and therefore displays a more restricted immunoreactivity. Because of its ability to stain routinely processed pathological tissues and its marked reactivity with human sarcomas, LN-6 is a unique reagent for the immunohistochemical diagnosis of human cancer.

scholarly journals REAP: A platform to identify autoantibodies that target the human exoproteome

2021 ◽  
Author(s):  
Eric Y. Wang ◽  
Yile Dai ◽  
Connor E. Rosen ◽  
Monica M. Schmitt ◽  
Mei X. Dong ◽  
...  
Keyword(s):  
Lupus Erythematosus ◽  
Wide Spectrum ◽  
Intrinsic Factor ◽  
Clinical Manifestations ◽  
Type I Interferons ◽  
Type I ◽  
Glycoprotein Hormone ◽  
Binding Event ◽  
Extracellular Matrix Components ◽  
Autoimmune Polyendocrinopathy

AbstractAutoantibodies that recognize extracellular protein epitopes (the “exoproteome”) exert potent functional effects that underlie numerous disease processes. Identifying these antibodies can thus provide insights into the pathophysiology of a wide spectrum of illnesses and therapeutic strategies to treat them. Here, we developed Rapid Extracellular Antigen Profiling (REAP) as a technique for comprehensive and high-throughput discovery of exoproteome-targeting autoantibodies. With REAP, patient samples are applied to a genetically-barcoded library containing 2,688 unique members of the human exoproteome displayed on the surface of yeast. Antibody-coated cells are isolated by magnetic selection and deep sequencing of their barcodes is used to identify the displayed antigens, thereby converting an antibody:antigen binding event into a digital sequencing readout. To benchmark the performance of REAP, we screened 77 patients with the rare monogenic autoimmune disease autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED). REAP sensitively and specifically detected known autoantibody reactivities in APECED, including responses against type I interferons, IL-17, IL-22, and gastric intrinsic factor. REAP also identified highly prevalent reactivities that had not been previously described such as those against the glycoprotein hormone GPHB5. We additionally screened 106 patients with systemic lupus erythematosus (SLE) and identified novel autoantibody reactivities against a diverse set of antigens including growth factors, extracellular matrix components, cytokines, and immunomodulatory proteins. Several of these responses were associated with disease severity and specific clinical manifestations of SLE, including autoantibodies that target immunoreceptors, antagonize the pro-inflammatory cytokine IL-33, and recognize endosialin (CD248) and the chemokine CCL8. In summary, these findings demonstrate the utility of REAP to atlas the expansive landscape of exoproteome-targeting autoantibodies in patients.

Lymphocyte subsets contribute to the degree of lobulitis and ductitis in sclerosing lymphocytic lobulitis of the breast

2015 ◽  
Vol 69 (6) ◽  
pp. 527-532 ◽  
Author(s):  
Lin-Ying Chen ◽  
Julia Y S Tsang ◽  
Yun-Bi Ni ◽  
Siu-Ki Chan ◽  
Kui-Fat Chan ◽  
...  
Keyword(s):  
T Lymphocyte ◽  
B Lymphocyte ◽  
Lymphocyte Subsets ◽  
Lymphocytic Infiltration ◽  
T Lymphocyte Subsets ◽  
Positive Correlations ◽  
Lymphocytic Lobulitis ◽  
Treg Lymphocytes ◽  
Infiltrating Lymphocytes ◽  
Cd4 Lymphocytes

AimsSclerosing lymphocytic lobulitis (SLL) of the breast is characterised by lymphocytic lobulitis, ductitis, vasculitis and dense keloidal fibrosis with epithelioid fibroblasts. However, the subsets of the infiltrating lymphocytes and their contribution to disease progression have not been fully explored.MethodsCD20, CD3, CD4, CD8 and regulatory T (Treg) lymphocytes were evaluated in the epithelial and vascular areas in SLL. The relationship between the lymphocyte subset in different regions and the degree of inflammation was analysed.ResultsLymphocytic infiltration was mainly located in peri-lobular, peri-ductal and peri-vascular areas. No significant differences between CD20 and CD3 lymphocytes were found in peri-epithelial areas. However, there were more intra-ductal/lobular epithelial CD3 than CD20 lymphocytes (p<0.001). For T lymphocyte subsets, more CD4 than CD8 lymphocytes were found in the peri-lobular/vascular regions (p≤0.026); but an opposite trend was seen in the intra-ductal/lobular regions (p<0.001). In the peri-lobular/vascular regions, generally, different lymphocyte subsets correlated with each other. Interestingly, in the peri-ductal region, only CD4 lymphocytes showed significant correlations with all other subsets (p≤0.020). Regarding their relationship with the degree of inflammation, significant positive correlations were observed for all subsets in peri-vascular/lobular regions (p≤0.045). Only regulatory T cells, but not the others, at the peri-ductal region showed significant correlation with the degree of inflammation at all three regions (p≤0.014).ConclusionsIn addition to B lymphocyte subsets, T lymphocyte subsets could be involved differently in SLL. CD4 lymphocytes may have a pivotal role in recruiting other subsets to the inflamed site, and triggered the cascade of inflammatory changes resulting in fibrosis.

Predominant intraepithelial localization of primed T cells and immunoglobulin-producing lymphocytes in Graves' disease

1991 ◽  
Vol 124 (6) ◽  
pp. 630-636 ◽  
Author(s):  
R. Paschke ◽  
N. Brückner ◽  
R. Schmeidl ◽  
P. Pfiester ◽  
K. H. Usadel
Keyword(s):  
T Cells ◽  
T Lymphocytes ◽  
Lymphocytic Infiltration ◽  
Intraepithelial Lymphocytes ◽  
Pokeweed Mitogen ◽  
Graves Disease ◽  
Immunoglobulin Synthesis ◽  
Thyroid Glands ◽  
Staining Index

Abstract. It has been proposed that intrathyroidal lymphocytes, localized in specific anatomical sites might have distinct, pathophysiologically relevant functions in Graves' disease. However, most studies of intrathyroidal lymphocytes were restricted to two lymphocyte locations and used semiquantitative methods. Therefore we used seven anatomically different lymphoid compartments to classify and evaluate by quantitative representative methods the total intrathyroidal lymphocytic infiltration and the staining indexes for immunoglobulin-producing plasmocytes and primed T cells (CD45RO), which provide maximum help to pokeweed mitogen-stimulated immunoglobulin synthesis in 36 thyroid glands from patients with Graves' disease. We found only 3.4% of all intrathyroidal lymphocytes intraepithelially. However, only intraepithelial lymphocytes showed a significantly higher staining index for primed T cells compared with several other compartments. There was also a high staining index for immunoglobulin-producing lymphocytes in this compartment. Kappa- and lambda-positive plasmocytes were found in a polyclonal distribution (kappa:lambda=64.1: 35.9) in all compartments. This increased incidence of CD45RO-positive T lymphocytes and of immunoglobulin-producing lymphocytes among the intraepithelial lymphocytes suggests a distinct pathophysiological function of lymphocytes in peripolesis in Graves' disease. Furthermore, there is a polyclonal intrathyroidal immunoglobulin synthesis.

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