HPV-16 (and possibly HPV-18) is different: implications for screening before and after an HPV vaccine

2005 ◽  
Vol 16 (6) ◽  
pp. 395-396
Author(s):  
Allan Hildesheim
Keyword(s):  
Hpv Vaccine ◽  
Hpv 16 ◽  
Before And After ◽  
Hpv 18

scholarly journals Immunogenicity of Quadrivalent HPV Vaccine in Adolescent Ghanaian Girls 36 Months After Vaccination

2021 ◽  
Author(s):  
Emmanuel Timmy Donkoh ◽  
Edward Tieru Dassah ◽  
Ellis Owusu-Dabo
Keyword(s):  
Adolescent Girls ◽  
Immunoglobulin G ◽  
Hpv Vaccine ◽  
Quadrivalent Vaccine ◽  
Enzyme Linked Immunosorbent Assay ◽  
Antibody Activity ◽  
Primary Role ◽  
Hpv 16 ◽  
Hpv 18

Abstract Introduction Available human papillomavirus (HPV) vaccines could have an important primary role in cervical cancer prevention once their long-term immunogenicity and safety are evaluated at the population level. The aim of this study was to optimize an assay to be used in evaluating the long-term durability of HPV vaccine response following a pilot vaccination of adolescent girls in Ghana. Methods A rapid, high-throughput, indirect enzyme-linked immunosorbent assay (ELISA) was evaluated for the detection and quantitation of anti-HPV L1 (late expression protein: types 6, 11, 16 and 18) immunoglobulin G (IgG) in human serum (n = 89). The performance of the assay was evaluated using serum collected from a cohort of pre-adolescent girls (n = 49) previously vaccinated with a quadrivalent vaccine and non-immune serum obtained from age-matched controls (n = 40). Results The seroprevalence of anti-HPV IgG antibodies was significantly higher among vaccinated than unvaccinated girls for both HPV − 16 (63.3% vs. 12.5%; p < 0.001) and HPV − 18 (34.7% vs. 20.0%; p = 0.042), respectively. Thirty-six months after receiving the third dose of vaccine, significantly higher mean anti–HPV-16 (0.618 vs. 0.145), anti–HPV-18 (0.323 vs. 0.309), and anti–HPV-6 (1.371 vs. 0.981) antibody levels were measured, compared to unvaccinated girls (all p < 0.05). A correlation between optical density and antibody activity indicated assay sensitivity to increasing levels of antibody activity. Conclusion We have successfully developed and implemented a robust and sensitive assay for the evaluation of antibody responses among immunized adolescent girls for monitoring future large-scale HPV vaccination studies in low-income settings. Our results demonstrated greater immunoglobulin G antibody activity within serum drawn from adolescent girls immunized 36 months prior.

scholarly journals Human Papillomavirus 16 (HPV 16) and HPV 18 Antibody Responses Measured by Pseudovirus Neutralization and Competitive Luminex Assays in a Two- versus Three-Dose HPV Vaccine Trial

2011 ◽  
Vol 18 (3) ◽  
pp. 418-423 ◽  
Author(s):  
Mel Krajden ◽  
Darrel Cook ◽  
Amanda Yu ◽  
Ron Chow ◽  
Wendy Mei ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Hpv Vaccine ◽  
Antibody Responses ◽  
Hpv 16 ◽  
Suitable Alternative ◽  
Human Papillomavirus 16 ◽  
Group 3 ◽  
Group 2 ◽  
Hpv 18 ◽  
Group 1

ABSTRACTHuman papillomavirus 16 (HPV 16) and HPV 18 antibody responses in a 2- versus 3-dose HPV vaccine (Gardasil) trial were measured by a pseudovirus neutralizing antibody (PsV NAb) assay and by the Merck competitive Luminex immunoassay (cLIA). Eight hundred twenty-four female subjects assigned to three dosing regimens (group 1, 9 to 13 years old; 2 doses, months 0 and 6 [n= 259]; group 2, 9 to 13 years old; 3 doses, months 0, 2, and 6 [n= 260]; group 3, 16 to 26 years old; 3 doses, months 0, 2, and 6 [n= 305]) had postvaccine responses assessed 1 month after the last dose. Of 791 subjects with baseline and 7-month sera, 15 (1.9%) and 9 (1.1%) were baseline seropositive for HPV 16 and HPV 18, respectively. All baseline-seronegative vaccinees seroconverted to both HPV 16 and HPV 18. Mean anti-HPV 16 levels were similar for groups 1 and 2 (for PsV NAb,P= 0.675; for cLIA,P= 0.874), and levels for both groups 1 and 2 were approximately 2-fold higher than that for group 3 (for PsV NAb and cLIA,P< 0.001). Mean anti-HPV 18 levels were approximately 1.4-fold lower in group 1 than in group 2 (for PsV, NAbP= 0.013; for cLIA,P= 0.001), and levels for both groups 1 and 2 were approximately 2.0- to 2.5-fold higher than that for group 3 (for PsV NAb and cLIA,P< 0.001). Pearson correlation coefficients for the assays were 0.672 for HPV 16 and 0.905 for HPV 18. Most of the discordant results were observed at lower cLIA signals. These results suggest that the PsV NAb assay could be a suitable alternative to cLIA for the measurement of postvaccine antibody responses.

Human papilloma virus (HPV) vaccine: Gardasil reactions

2014 ◽  
Vol 2 (3) ◽  
pp. 137-146
Keyword(s):  
United States ◽  
Cervical Cancer ◽  
Hpv Vaccine ◽  
Death Rate ◽  
The United States ◽  
Hpv Infection ◽  
World Health ◽  
Hpv 16 ◽  
Night Sweats ◽  
Hpv 18

The World Health Organization (WHO) as well as public health officials in Australia, Canada, Europe, and the United States recommend vaccination of young women against HPV to prevent cervical cancer, and to reduce the number of treatments for cervical cancer precursors. The death rate from cervical cancer in the United States is 3 per 100,000 and in 2011, about 12,000 women were diagnosed with cervical cancer and 4,000 died. In 2009, there were about 34,000 deaths from car accidents in the U.S. for a death rate of 11 per 100,000. HPV infection is extremely common; most sexually active people will be infected with HPV at some point in life. HPV infection usually causes no symptoms, but can cause genital warts and anal cancer in both women and men. The HPV vaccine prevents infection by the HPV types responsible for most cervical cancers. There are two available forms of the HPV vaccine: Cervarix; prevents infection by HPV-16 and HPV-18 and Gardasil; prevents infection by HPV-16, HPV-18, and also HPV-6 and HPV-11. There are many side events may caused by HPV vaccines such as; seizures, fatigue, strokes, dizziness, weakness, headaches, stomach pains, vomiting, muscle pain and weakness, joint pain, auto-immune problems, chest pains, hair loss, appetite loss, shortness of breath, heart problems, personality changes, insomnia, hand/leg tremors, arm/leg weakness, paralysis, itching, rashes, swelling, aching muscles, pelvic pain, nerve pain, menstrual cycle changes, fainting, swollen lymph nodes, night sweats, nausea, temporary vision/hearing loss. This topic review focused mainly on the side effects and health serious problems with Gardasil vaccine.

HPV 16 und HPV 18 im Risikokollektiv einer Dysplasiesprechstunde in der Steiermark

2008 ◽  
Vol 68 (04) ◽  
Author(s):  
M Ballon ◽  
O Reich ◽  
R Winter ◽  
U Lang
Keyword(s):  
Hpv 16 ◽  
Hpv 18

PECULIARITIES OF PAPILLOMAVIRUS GENOTYPING IN PATIENTS WITH CERVICAL INTRAEPITHELIAL NEOPLASIA

2020 ◽  
pp. 104-111
Author(s):  
N.A. Shmakova ◽  
G.N. Chistyakova ◽  
I.N. Kononova ◽  
I.I. Remizova
Keyword(s):  
Cervical Cancer ◽  
Human Papillomavirus ◽  
Viral Load ◽  
Cervical Intraepithelial Neoplasia ◽  
Intraepithelial Neoplasia ◽  
Squamous Intraepithelial Lesions ◽  
Hpv 16 ◽  
The World ◽  
Intraepithelial Lesions ◽  
Hpv 18

Recently, there has been a steady growth of cervical cancer all over the world, especially in Russia. Patients with cervical cancer have become much younger. At the same time, the human papillomavirus is not only the main factor in the neoplastic process, but it is also one of the most common sexually transmitted infections in the world. The aim of the paper is to assess the prevalence and characteristics of human papillomavirus genotypes in patients with cervical intraepithelial neoplasia. Materials and Methods. During the periodic screening we examined 213 women of a reproductive age with HPV infection. All patients underwent liquid-based cytology and human papillomavirus genotyping by polymerase chain reaction. Results. We revealed that the prevalence of cervical intraepithelial neoplasia among women with papillomavirus infection was 80.3 % (n=171). According to human papillomavirus genotyping, HPV 16 (38 %) and HPV 33 (32 %) prevailed. We also observed positive high correlation between high-grade squamous intraepithelial lesions (HSIL) and HPV 18 (r=+0.759, p=0.001), a negative mean correlation between HPV 45 and low-grade squamous intraepithelial lesions (LSIL) (r=-0.643, p=0.002). A cohort of patients with severe intraepithelial cervical lesions demonstrated high viral load rates. Conclusion. According to the results obtained, we established the dominance of HPV 16 and HPV 33 genotypes in cervical intraepithelial neoplasia. There were significant differences between HSIL and LSIL patients with HPV 18 and HPV 45. There was also a correlation between an increase in the viral load with the severity of the pathological process. Keywords: human papillomavirus, intraepithelial cervical neoplasms, cervical cancer. В последние годы в мире, особенно в России, наблюдается неуклонный рост и «омолаживание» рака шейки матки. При этом вирус папилломы человека является не только основным фактором прогрессирования неопластического процесса, но и одной из наиболее распространенных инфекций, предаваемых половым путем, в мире. Цель. Оценить распространенность и характеристику генотипов папилломавирусной инфекции у пациенток с цервикальными интраэпителиальными неоплазиями. Материалы и методы. Проведено обследование 213 пациенток репродуктивного возраста с ВПЧ-инфекцией, пришедших на профилактический осмотр. Всем женщинам было выполнено цитологическое исследование жидкостным методом и генотипирование вируса папилломы человека методом полимеразной цепной реакции. Результаты. Распространенность цервикальных интраэпителиальных неоплазий среди женщин с папилломавирусной инфекцией составила 80,3 % (171 пациентка). Согласно данным генотипирования вируса папилломы человека превалировал 16-й (38 %) и 33-й типы (32 %). Выявлена положительная высокая корреляционная связь между цервикальными неоплазиями высокой степени онкогенного риска (HSIL) и 18-м типом ВПЧ-инфекции (r=+0,759 при р=0,001), отрицательная средняя корреляционная связь 45-го типа ВПЧ с низкой степенью онкогенного риска (LSIL) (r=-0,643 при р=0,002). Продемонстрированы высокие показатели вирусной нагрузки в когорте пациенток с тяжелыми внутриэпителиальными цервикальными поражениями. Выводы. По результатам полученных данных установлено доминирование 16-го и 33-го генотипов ВПЧ при цервикальных интраэпителиальных неоплазиях с наличием значимых различий между пациентами с HSIL и LSIL в отношении 18-го и 45-го типов, а также связь роста уровня вирусной нагрузки с увеличением степени тяжести патологического процесса. Ключевые слова: вирус папилломы человека, интраэпителиальные новообразования шейки матки, рак шейки матки.

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