Apoptosis induction in gastric mucous cells in vitro: lesser potency of Helicobacter pylori than Escherichia coli lipopolysaccharide, but positive interaction with ibuprofen

In Escherichia coli, DnaC is essential for loading DnaB helicase at oriC (the origin of chromosomal DNA replication). The question arises as to whether this model can be generalized to other species, since many eubacterial species fail to possess dnaC in their genomes. Previously, we have reported the characterization of HpDnaB (Helicobacter pylori DnaB) both in vitro and in vivo. Interestingly, H. pylori does not have a DnaC homologue. Using two different E. coli dnaC (EcdnaC) temperature-sensitive mutant strains, we report here the complementation of EcDnaC function by HpDnaB in vivo. These observations strongly suggest that HpDnaB can bypass EcDnaC activity in vivo.

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Prostaglandin E2 alters terminal glycosylation of high molecular weight glycoproteins, released by pig gastric mucous cells in vitro

Prostaglandins Leukotrienes and Essential Fatty Acids ◽

10.1016/0952-3278(95)90035-7 ◽

1995 ◽

Vol 52 (5) ◽

pp. 333-340 ◽

Cited By ~ 11

Author(s):

M.-L. Enss ◽

U. Schmidt-Wittig ◽

H.-K. Heim ◽

K.-F. Sewing

Keyword(s):

Molecular Weight ◽

Prostaglandin E2 ◽

High Molecular Weight ◽

Mucous Cells ◽

Gastric Mucous

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Helicobacter pylori infection produces expression of secretory component in gastric mucous cells

Gastroenterology ◽

10.1016/s0016-5085(08)83528-7 ◽

2001 ◽

Vol 120 (5) ◽

pp. A708-A709

Author(s):

Taimei Kaneko ◽

Hiroyoshi Ota ◽

Masayoshi Hayama ◽

Kosei Nakajima ◽

Akihiko Yoshizawa ◽

...

Keyword(s):

Helicobacter Pylori ◽

Secretory Component ◽

Helicobacter Pylori Infection ◽

Mucous Cells ◽

Gastric Mucous

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Inhibitory Effect of Heterologous Ribosome Recycling Factor on Growth of Escherichia coli

Journal of Bacteriology ◽

10.1128/jb.182.21.6154-6160.2000 ◽

2000 ◽

Vol 182 (21) ◽

pp. 6154-6160 ◽

Cited By ~ 6

Author(s):

Kenji Atarashi ◽

Akira Kaji

Keyword(s):

Escherichia Coli ◽

Helicobacter Pylori ◽

Thermotoga Maritima ◽

Inhibitory Effect ◽

Dependent Manner ◽

Ribosome Recycling Factor ◽

E Coli ◽

Genes Encoding ◽

Dose Dependent

ABSTRACT Ribosome recycling factor (RRF) of Thermotoga maritimawas expressed in Escherichia coli from the cloned T. maritima RRF gene and purified. Expression of T. maritima RRF inhibited growth of the E. coli host in a dose-dependent manner, an effect counteracted by the overexpression of E. coli RRF. T. maritima RRF also inhibited the E. coli RRF reaction in vitro. Genes encoding RRFs fromStreptococcus pneumoniae and Helicobacter pylori have been cloned, and they also impair growth of E. coli, although the inhibitory effect of these RRFs was less pronounced than that of T. maritima RRF. The amino acid sequence at positions 57 to 62, 74 to 78, 118 to 122, 154 to 160, and 172 to 176 in T. maritima RRF differed totally from that ofE. coli RRF. This suggests that these regions are important for the inhibitory effect of heterologous RRF. We further suggest that bending and stretching of the RRF molecule at the hinge between two domains may be critical for RRF activity and therefore responsible forT. maritima RRF inhibition of the E. coli RRF reaction.

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