Effects of Microwave Fixation and Decalcification on Rodent Tissue

2009 ◽  
Vol 32 (4) ◽  
pp. 190-192 ◽  
Author(s):  
April L. Marr ◽  
Anthony Wong
Keyword(s):  
Microwave Fixation

Opening images of synaptic vesicles and calcium localization by means of microwave fixation method

1990 ◽  
Vol 48 (2) ◽  
pp. 182-183
Author(s):  
Vinci Mizuhira ◽  
Hiroshi Hasegawa
Keyword(s):  
Synaptic Vesicles ◽  
Room Temperature ◽  
Osmium Tetroxide ◽  
Sampling Procedure ◽  
Fixation Method ◽  
Potassium Oxalate ◽  
X Ray ◽  
Cacodylate Buffer ◽  
Ultrathin Sections ◽  
Microwave Fixation

Microwave irradiation (MWI) was applied to 0.3 to 1 cm3 blocks of rat central nervous system at 2.45 GHz/500W for about 20 sec in a fixative, at room temperature. Fixative composed of 2% paraformaldehyde, 0.5% glutaraldehyde in 0.1 M cacodylate buffer at pH 7.4, also contained 2 mM of CaCl2 , 1 mM of MgCl2, and 0.1% of tannic acid for conventional observation; and fuether 30-90 mM of potassium oxalate containing fixative was applied for the detection of calcium ion localization in cells. Tissue blocks were left in the same fixative for 30 to 180 min after MWI at room temperature, then proceeded to the sampling procedure, after postfixed with osmium tetroxide, embedded in Epon. Ultrathin sections were double stained with an useal manner. Oxalate treated sections were devided in two, stained and unstained one. The later oxalate treated unstained sections were analyzed with electron probe X-ray microanalyzer, the EDAX-PU-9800, at 40 KV accelerating voltage for 100 to 200 sec with point or selected area analyzing methods.

A review of rapid microwave fixation technology: Its expanding niche in morphologic studies

Scanning ◽  
1993 ◽  
Vol 15 (2) ◽  
pp. 58-66 ◽  
Author(s):  
Gary R. Login ◽  
Ann M. Dvorak
Keyword(s):  
Microwave Fixation

REGIONAL ?-AMINOBUTYRIC ACID LEVELS IN RAT BRAIN DETERMINED AFTER MICROWAVE FIXATION

1975 ◽  
Vol 24 (4) ◽  
pp. 609-613 ◽  
Author(s):  
G. J. Balcom ◽  
R. H. Lenox ◽  
J. L. Meyerhoff
Keyword(s):  
Rat Brain ◽  
Aminobutyric Acid ◽  
Microwave Fixation

Rapid microwave fixation?a comparative morphometric study

1988 ◽  
Vol 20-20 (6-7) ◽  
pp. 347-352 ◽  
Author(s):  
K. Kayser ◽  
H. Stute ◽  
J. L�bcke ◽  
U. Wazinski
Keyword(s):  
Morphometric Study ◽  
Microwave Fixation

EVIDENCE FOR A NOVEL INHIBITION OF CALCIUM UPTAKE INTO CHICK BONE IN RESPONSE TO BOVINE PARATHYROID HORMONE (1-34) OR 16, 16-DIMETHYL PROSTAGLANDIN E2 IN VIVO

1985 ◽  
Vol 105 (3) ◽  
pp. R5-R8 ◽  
Author(s):  
A.J. Shaw ◽  
C.G. Dacke
Keyword(s):  
Parathyroid Hormone ◽  
Soft Tissue ◽  
Calcium Uptake ◽  
Separate Experiment ◽  
Response Curves ◽  
45Ca Uptake ◽  
Microwave Fixation ◽  
Absolute Basis ◽  
Bovine Parathyroid Hormone

ABSTRACT Mechanisms of initial hypercalcaemic responses to parathyroid hormone (PTH) and 16, 16-dimethyl prostaglandin (PG) E2 have been investigated in 10-to 12-day-old chicks in vivo using a combination of acute 45Ca injection and microwave fixation to stabilize tissue isotope levels. Single i.v. injection of 16, 16-dimethyl PGE2 (20 μg/100 g body wt) caused an approximately 100% increase in soft tissue 45Ca levels compared with vehicle control injected chicks at 30 min. 45Ca levels were lowered in calvarium by 26% and in femur by 60% with this treatment. Bovine PTH (1-34) (3.3 μg/100 g body wt) had no effect on soft tissue 45Ca levels, but in calvarium it had a similar effect to the PG. In femur this dose of PTH lowered 45Ca by 19%. When expressed on an absolute basis (c.p.m./ 100 mg tissue wt), responses to the PG in soft tissue were only 3 and 10% respectively of those in femur and calvarium. The duration of inhibitory responses in bone were examined and those to PTH found to be transient (< 45 min) compared with the responses to the PG (> 135 min). Dose-response curves for PTH- and PG-induced inhibition of 45Ca uptake into femur at 15 min were essentially parallel and indicated that the lowest doses of PTH and PG used (0.74 μg and 1.1 μg/100 body wt respectively) produced significant responses. In a separate experiment it was found that inhibition of 45Ca uptake into femur was evident as early as 3 min following PTH or PG injection. The experiments described in this paper collectively indicate a higherto unrecognized action of PTH and PGE in the regulation of Ca metabolism in chicks which is to inhibit entry of Ca into bone.

Regional glutamate levels in rat brain determined after microwave fixation

1976 ◽  
Vol 26 (2) ◽  
pp. 423-425 ◽  
Author(s):  
G. J. Balcom ◽  
R. H. Lenox ◽  
J. L. Meyerhoff
Keyword(s):  
Rat Brain ◽  
Microwave Fixation

Microwave fixation of cells in culture for scanning electron microscopy

1983 ◽  
Vol 41 ◽  
pp. 630-631
Author(s):  
E.C. Chew ◽  
D.J. Riches ◽  
P.P.L. Tam ◽  
G.S.W. Tsao ◽  
T.K. Lam ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cell Suspension ◽  
Single Cell ◽  
Culture Medium ◽  
Single Cell Suspension ◽  
Electron Microscopic ◽  
Syringe Needle ◽  
Microwave Fixation ◽  
Scanning Electron

The use of microwave irradiation for fixation of human and animal tissue has been proven satisfactory at light microscopic and electron microscopic levels. The present communication reports the study of the same method of fixation of cell cultures for scanning electron microscopy.Trophoblasts were isolated from the placentae of mouse conceptuses at 10.5 days of gestation. The placenta was dissected out from the decidua and placed in Ca and Mg-free PBS, minced and then forced through a gauge-21 syringe needle. The tissue fragments were digested with 0.25% trypsin in Ca and Mg-free PBS for 20 - 30 minutes at 4°C. The digested tissue was then washed with complete PB1 medium. A single-cell suspension was obtained by spinning down the larger fragments by centrifugation. A known volume of the single-cell suspension was added to the culture medium (DCMEM and 20% FCS). The culture medium was changed after 24 hours to remove any unattached cells.

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