Lentivirus vectors expressing short hairpin RNAs against the U3-overlapping region of HIV nef inhibit HIV replication and infectivity in primary macrophages

Blood ◽  
2006 ◽  
Vol 108 (10) ◽  
pp. 3305-3312 ◽  
Author(s):  
T. Yamamoto ◽  
H. Miyoshi ◽  
N. Yamamoto ◽  
N. Yamamoto ◽  
J.-i. Inoue ◽  
...  
Keyword(s):  
Hiv Replication ◽  
Short Hairpin ◽  
Lentivirus Vectors ◽  
Short Hairpin Rnas ◽  
Overlapping Region

scholarly journals Large-Scale, High-Throughput Validation of Short Hairpin RNA Sequences for RNA Interference

2006 ◽  
Vol 11 (3) ◽  
pp. 236-246 ◽  
Author(s):  
Laurence H. Lamarcq ◽  
Bradley J. Scherer ◽  
Michael L. Phelan ◽  
Nikolai N. Kalnine ◽  
Yen H. Nguyen ◽  
...  
Keyword(s):  
High Throughput ◽  
Large Scale ◽  
Strong Support ◽  
Gc Content ◽  
Rapid Identification ◽  
Hairpin Rna ◽  
Rna Sequences ◽  
Short Hairpin ◽  
Short Hairpin Rnas ◽  
Interfering Rna

A method for high-throughput cloning and analysis of short hairpin RNAs (shRNAs) is described. Using this approach, 464 shRNAs against 116 different genes were screened for knockdown efficacy, enabling rapid identification of effective shRNAs against 74 genes. Statistical analysis of the effects of various criteria on the activity of the shRNAs confirmed that some of the rules thought to govern small interfering RNA (siRNA) activity also apply to shRNAs. These include moderate GC content, absence of internal hairpins, and asymmetric thermal stability. However, the authors did not find strong support for positionspecific rules. In addition, analysis of the data suggests that not all genes are equally susceptible to RNAinterference (RNAi).

scholarly journals Optimized RNA-targeting CRISPR/Cas13d technology outperforms shRNA in identifying functional circRNAs

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Yang Zhang ◽  
Tuan M. Nguyen ◽  
Xiao-Ou Zhang ◽  
Limei Wang ◽  
Tin Phan ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
High Throughput ◽  
Biological Effect ◽  
Human Hepatocellular Carcinoma ◽  
Guide Rnas ◽  
Rna Targeting ◽  
Short Hairpin ◽  
Short Hairpin Rnas ◽  
Screening Library ◽  
High Throughput Study

AbstractShort hairpin RNAs (shRNAs) are used to deplete circRNAs by targeting back-splicing junction (BSJ) sites. However, frequent discrepancies exist between shRNA-mediated circRNA knockdown and the corresponding biological effect, querying their robustness. By leveraging CRISPR/Cas13d tool and optimizing the strategy for designing single-guide RNAs against circRNA BSJ sites, we markedly enhance specificity of circRNA silencing. This specificity is validated in parallel screenings by shRNA and CRISPR/Cas13d libraries. Using a CRISPR/Cas13d screening library targeting > 2500 human hepatocellular carcinoma-related circRNAs, we subsequently identify a subset of sorafenib-resistant circRNAs. Thus, CRISPR/Cas13d represents an effective approach for high-throughput study of functional circRNAs.

A quick and efficient approach for gene silencing by using triple putative microRNA-based short hairpin RNAs

2008 ◽  
Vol 323 (1-2) ◽  
pp. 81-89 ◽  
Author(s):  
Z. X. Shan ◽  
Q. X. Lin ◽  
M. Yang ◽  
C. Y. Deng ◽  
S. J. Kuang ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Efficient Approach ◽  
Short Hairpin ◽  
Short Hairpin Rnas

scholarly journals Publisher Correction: Gene knockdown via electroporation of short hairpin RNAs in embryos of the marine hydroid Hydractinia symbiolongicarpus

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Gonzalo Quiroga-Artigas ◽  
Alexandrea Duscher ◽  
Katelyn Lundquist ◽  
Justin Waletich ◽  
Christine E. Schnitzler
Keyword(s):  
Gene Knockdown ◽  
Hydractinia Symbiolongicarpus ◽  
Short Hairpin ◽  
Short Hairpin Rnas

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

scholarly journals Generation of human pluripotent stem cell lines with suppressed expression of the Notch ligand DLL4 using short hairpin RNAs

2016 ◽  
Vol 16 (3) ◽  
pp. 735-739
Author(s):  
Federico González-Pozas ◽  
Rosa Montes ◽  
Lourdes López-Onieva ◽  
Tamara Romero ◽  
Joan Domingo-Reinés ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Lines ◽  
Pluripotent Stem Cell ◽  
Human Pluripotent Stem Cell ◽  
Notch Ligand ◽  
Short Hairpin ◽  
Stem Cell Lines ◽  
Short Hairpin Rnas

UP-3.003: Antitumor Activity of Conditionally Replicating Adenoviruses Expressing KI67-Specific Short Hairpin RNAs for Bladder Cancer

Urology ◽  
2009 ◽  
Vol 74 (4) ◽  
pp. S295-S296
Author(s):  
J. Zheng ◽  
J. Liu ◽  
L. Mao ◽  
W. Li ◽  
F. Sun ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Antitumor Activity ◽  
Short Hairpin ◽  
Short Hairpin Rnas

scholarly journals A versatile approach to multiple gene RNA interference using microRNA-based short hairpin RNAs

2007 ◽  
Vol 8 (1) ◽  
pp. 98 ◽  
Author(s):  
Xiaocui Zhu ◽  
Leah A Santat ◽  
Mi Chang ◽  
Jamie Liu ◽  
Joelle R Zavzavadjian ◽  
...  
Keyword(s):  
Rna Interference ◽  
Multiple Gene ◽  
Short Hairpin ◽  
Short Hairpin Rnas

scholarly journals Intravenous liposomal delivery of the short hairpin RNAs against Plk1 controls the growth of established human hepatocellular carcinoma

2011 ◽  
Vol 11 (4) ◽  
pp. 401-409 ◽  
Author(s):  
Hongxin Deng ◽  
Qingyuan Jiang ◽  
Yang Yang ◽  
Shuang Zhang ◽  
Yongping Ma ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Human Hepatocellular Carcinoma ◽  
Short Hairpin ◽  
Liposomal Delivery ◽  
Short Hairpin Rnas
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