scholarly journals Spatial gradients of blood vessels and hematopoietic stem and progenitor cells within the marrow cavities of the human skeleton

Blood ◽  
2009 ◽  
Vol 114 (19) ◽  
pp. 4077-4080 ◽  
Author(s):  
Vincent A. Bourke ◽  
Christopher J. Watchman ◽  
John D. Reith ◽  
Marda L. Jorgensen ◽  
Arnaud Dieudonnè ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Relative Concentration ◽  
Hematopoietic Stem ◽  
Spatial Gradients ◽  
Human Cancellous Bone ◽  
Close Proximity ◽  
Marrow Space ◽  
Stem And Progenitor Cells ◽  
Spatial Niche ◽  
Trabecular Surface

Abstract This report evaluates the spatial profile of blood vessel fragments (BVFs) and CD34+ and CD117+ hematopoietic stem and progenitor cells (HSPCs) in human cancellous bone. Bone specimens were sectioned, immunostained (anti-CD34 and anti-CD117), and digitally imaged. Immunoreactive cells and vessels were then optically and morphometrically identified and labeled on the corresponding digital image. The distance of each BVF, or CD34+ or CD117+ HSPC to the nearest trabecular surface was measured and binned in 50-μm increments. The relative concentration of HSPCs and BVFs within cancellous marrow was observed to diminish with increasing distance in the marrow space. On average, 50% of the CD34+ HSPC population, 60% of the CD117+ HSPC population, and 72% of the BVFs were found within 100 μm of the bone surfaces. HSPCs were also found to exist in close proximity to BVFs, which supports the notion of a shared HSPC and vessel spatial niche.

Flow cytometric enumeration and immunophenotyping of hematopoietic stem and progenitor cells

2001 ◽  
Vol 38 (2) ◽  
pp. 139-147
Author(s):  
Jan W. Gratama ◽  
D. Robert Sutherland ◽  
Michael Keeney
Keyword(s):  
Progenitor Cells ◽  
Hematopoietic Stem ◽  
Flow Cytometric ◽  
Stem And Progenitor Cells

Enhanced thrombin/PAR1 activity promotes G-CSF- and AMD3100-induced mobilization of hematopoietic stem and progenitor cells via NO upregulation

Leukemia ◽  
2021 ◽  
Author(s):  
Neta Nevo ◽  
Lizeth-Alejandra Ordonez-Moreno ◽  
Shiri Gur-Cohen ◽  
Francesca Avemaria ◽  
Suditi Bhattacharya ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Hematopoietic Stem ◽  
Stem And Progenitor Cells

3026 – METABOLIC ADAPTATION IN HEMATOPOIETIC STEM AND PROGENITOR CELLS DURING EMERGENCY MYELOPOIESIS

2020 ◽  
Vol 88 ◽  
pp. S46
Author(s):  
Oakley Olson ◽  
Fernando Calero-Nieto ◽  
Xiaonan Wang ◽  
Bethold Göttgens ◽  
Emmanuelle Passegué
Keyword(s):  
Progenitor Cells ◽  
Metabolic Adaptation ◽  
Hematopoietic Stem ◽  
Stem And Progenitor Cells ◽  
Emergency Myelopoiesis

3008 – OXYGEN TENSION REGULATES CALCIUM SIGNALING AND FUNCTION IN HEMATOPOIETIC STEM AND PROGENITOR CELLS

2020 ◽  
Vol 88 ◽  
pp. S40
Author(s):  
Paige Dausinas ◽  
Jacob Slack ◽  
Christopher Basile ◽  
Anish Karlapudi ◽  
Heather O'Leary
Keyword(s):  
Calcium Signaling ◽  
Progenitor Cells ◽  
Oxygen Tension ◽  
Hematopoietic Stem ◽  
Stem And Progenitor Cells ◽  
And Function

scholarly journals Ex vivo expansion of human hematopoietic stem and progenitor cells

Blood ◽  
2011 ◽  
Vol 117 (23) ◽  
pp. 6083-6090 ◽  
Author(s):  
Ann Dahlberg ◽  
Colleen Delaney ◽  
Irwin D. Bernstein
Keyword(s):  
Stem Cell ◽  
Growth Factors ◽  
Notch Signaling ◽  
Progenitor Cells ◽  
Ex Vivo ◽  
Ex Vivo Expansion ◽  
Hematopoietic Growth Factors ◽  
Self Renewal ◽  
Hematopoietic Stem ◽  
Stem And Progenitor Cells

AbstractDespite progress in our understanding of the growth factors that support the progressive maturation of the various cell lineages of the hematopoietic system, less is known about factors that govern the self-renewal of hematopoietic stem and progenitor cells (HSPCs), and our ability to expand human HSPC numbers ex vivo remains limited. Interest in stem cell expansion has been heightened by the increasing importance of HSCs in the treatment of both malignant and nonmalignant diseases, as well as their use in gene therapy. To date, most attempts to ex vivo expand HSPCs have used hematopoietic growth factors but have not achieved clinically relevant effects. More recent approaches, including our studies in which activation of the Notch signaling pathway has enabled a clinically relevant ex vivo expansion of HSPCs, have led to renewed interest in this arena. Here we briefly review early attempts at ex vivo expansion by cytokine stimulation followed by an examination of our studies investigating the role of Notch signaling in HSPC self-renewal. We will also review other recently developed approaches for ex vivo expansion, primarily focused on the more extensively studied cord blood–derived stem cell. Finally, we discuss some of the challenges still facing this field.

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