Convergent differentiation: myeloid and lymphoid pathways to murine plasmacytoid dendritic cells

Blood ◽  
2013 ◽  
Vol 121 (1) ◽  
pp. 11-19 ◽  
Author(s):  
Priyanka Sathe ◽  
David Vremec ◽  
Li Wu ◽  
Lynn Corcoran ◽  
Ken Shortman
Keyword(s):  
Dendritic Cells ◽  
B Cell ◽  
Plasmacytoid Dendritic Cells ◽  
Gene Rearrangements ◽  
Flt3 Ligand ◽  
Cell Potential ◽  
Cpg Oligonucleotides ◽  
Igh Genes ◽  
Transient Intermediate ◽  
Variable Efficiency

Abstract The developmental origin of IFN-producing plasmacytoid dendritic cells (pDCs) has been uncertain. In the present study, we tracked the development of pDCs in cultures of BM precursors stimulated with Flt3 ligand. Common myeloid precursors (CMPs) produced both conventional DCs (cDCs) and pDCs via the DC-restricted common DC precursor. Common lymphoid precursors (CLPs) produced only a few cDCs with variable efficiency, but produced pDCs via a transient intermediate precursor with B-cell potential. The pDCs of both origins produced IFN-α when stimulated with CpG oligonucleotides. The pDCs of CLP origin showed evidence of past RAG1 expression and had D-J rearrangements in IgH genes. Most pDCs and all cDCs of CMP origin lacked these signs of a lymphoid past. However, in these cultures, some pDCs of CMP origin showed evidence of past RAG1 expression and had D-J IgH gene rearrangements; most of these derived from a subset of CMPs already expressing RAG1.

scholarly journals Rational design of new CpG oligonucleotides that combine B cell activation with high IFN-α induction in plasmacytoid dendritic cells

2003 ◽  
Vol 33 (6) ◽  
pp. 1633-1641 ◽  
Author(s):  
Gunther Hartmann ◽  
Julia Battiany ◽  
Hendrik Poeck ◽  
Moritz Wagner ◽  
Miren Kerkmann ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
B Cell ◽  
Rational Design ◽  
Cell Activation ◽  
Plasmacytoid Dendritic Cells ◽  
B Cell Activation ◽  
Cpg Oligonucleotides

scholarly journals Human Cytomegalovirus Differentially Controls B Cell and T Cell Responses through Effects on Plasmacytoid Dendritic Cells

2007 ◽  
Vol 179 (11) ◽  
pp. 7767-7776 ◽  
Author(s):  
Stefania Varani ◽  
Madeleine Cederarv ◽  
Sari Feld ◽  
Charlotte Tammik ◽  
Giada Frascaroli ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
T Cell ◽  
B Cell ◽  
Human Cytomegalovirus ◽  
T Cell Responses ◽  
Plasmacytoid Dendritic Cells ◽  
Cell Responses

scholarly journals Separation of Plasmacytoid Dendritic Cells from B-Cell-Biased Lymphoid Progenitor (BLP) and Pre-Pro B Cells Using PDCA-1

PLoS ONE ◽  
2013 ◽  
Vol 8 (10) ◽  
pp. e78408 ◽  
Author(s):  
Kay L. Medina ◽  
Sarah N. Tangen ◽  
Lauren M. Seaburg ◽  
Puspa Thapa ◽  
Kimberly A. Gwin ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
B Cells ◽  
B Cell ◽  
Plasmacytoid Dendritic Cells

scholarly journals A distinct subset of plasmacytoid dendritic cells induces activation and differentiation of B and T lymphocytes

2017 ◽  
Vol 114 (8) ◽  
pp. 1988-1993 ◽  
Author(s):  
Hong Zhang ◽  
Josh D. Gregorio ◽  
Toru Iwahori ◽  
Xiangyue Zhang ◽  
Okmi Choi ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Cell Activation ◽  
Plasmacytoid Dendritic Cells ◽  
T Cell Proliferation ◽  
Type I ◽  
Type I Ifn ◽  
Immune Functions ◽  
B Cell Activation ◽  
Cpg Oligonucleotides ◽  
B And T Lymphocytes

Plasmacytoid dendritic cells (pDCs) are known mainly for their secretion of type I IFN upon viral encounter. We describe a CD2hiCD5+CD81+pDC subset, distinguished by prominent dendrites and a mature phenotype, in human blood, bone marrow, and tonsil, which can be generated from CD34+progenitors. These CD2hiCD5+CD81+cells express classical pDC markers, as well as the toll-like receptors that enable conventional pDCs to respond to viral infection. However, their gene expression profile is distinct, and they produce little or no type I IFN upon stimulation with CpG oligonucleotides, likely due to their diminished expression of IFN regulatory factor 7. A similar population of CD5+CD81+pDCs is present in mice and also does not produce type I IFN after CpG stimulation. In contrast to conventional CD5−CD81−pDCs, human CD5+CD81+pDCs are potent stimulators of B-cell activation and antibody production and strong inducers of T-cell proliferation and Treg formation. These findings reveal the presence of a discrete pDC population that does not produce type I IFN and yet mediates important immune functions previously attributed to all pDCs.

scholarly journals IFN-α produced by human plasmacytoid dendritic cells enhances T cell-dependent naïve B cell differentiation

2011 ◽  
Vol 89 (6) ◽  
pp. 811-821 ◽  
Author(s):  
Cornelia Gujer ◽  
Kerrie J. Sandgren ◽  
Iyadh Douagi ◽  
William C. Adams ◽  
Christopher Sundling ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Cell Differentiation ◽  
T Cell ◽  
B Cell ◽  
Plasmacytoid Dendritic Cells ◽  
B Cell Differentiation
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