scholarly journals Studies on the Serum Haptoglobin Level in Hemoglobinemia and Its Influence on Renal Excretion of Hemoglobin

Blood ◽  
1957 ◽  
Vol 12 (6) ◽  
pp. 493-506 ◽  
Author(s):  
CARL-BERTIL LAURELL ◽  
MARGARETA NYMAN
Keyword(s):  
Intravenous Injection ◽  
Threshold Value ◽  
Free Hemoglobin ◽  
Electrophoretic Method ◽  
Serum Haptoglobin ◽  
Hemoglobin Binding ◽  
Constant Rate ◽  
Haptoglobin Level ◽  
Binding Power

Abstract A short survey is given of the literature on haptoglobin, the hemoglobin-binding serum protein, its properties and biologic variations. The principles of an electrophoretic method for quantitative determination of the serum haptoglobin are described. Electrophoretic studies showed that haptoglobin has a high affinity for hemoglobin at physiologic pH and that every haptoglobin molecule can bind at least 2 hemoglobin molecules. Observations made following the intravenous injection of hemoglobin showed: that hemoglobin administered intravenously is bound by the haptoglobin; that free hemoglobin is not demonstrable until more hemoglobin has been injected than can be bound by the haptoglobin; that the complex hemoglobin-haptoglobin is eliminated from the plasma after intravascular hemolysis or intravenous administration of hemoglobin without being excreted in the urine; that the hemoglobin-haptoglobin complex is removed from the plasma at a constant rate during the major part of the elimination period; that the haptoglobin level will fall to nil within 24 hours, if the amount of hemoglobin injected is sufficient to bind all the haptoglobin available. During the following days the rate of formation of haptoglobin can be studied. From the data available it can be concluded that hemoglobinuria cannot appear until the amount of hemoglobin administered intravenously or the amount liberated intravascularly exceeds the binding power of the haptoglobin and the reabsorption capacity of the tubules. The variation observed by earlier authors in the so-called renal threshold for hemoglobin on intravenous injection of hemoglobin can be explained among other things by the variation in the haptoglobin content in one and the same subject, i.e., if the haptoglobin level is low, the threshold value will also be low, and vice versa.

Evaluation of a nephelometric assay for haptoglobin and its clinical usefulness.

1979 ◽  
Vol 25 (12) ◽  
pp. 2007-2010 ◽  
Author(s):  
F Van Lente ◽  
A Marchand ◽  
R S Galen
Keyword(s):  
Correlation Coefficient ◽  
Coefficient Of Variation ◽  
Binding Capacity ◽  
Clinical Correlation ◽  
Intravascular Hemolysis ◽  
Hemolytic Disease ◽  
Serum Haptoglobin ◽  
Hemoglobin Binding ◽  
Significant Interference

Abstract Serum haptoglobin has been advocated as an indicator of intravascular hemolysis. We have evaluated a nephelometric determination of serum haptoglobin. The assay is sensitive and exhibits within-run precision in the range of 2.5-7.4% coefficient of variation (CV) and between-run precision of 7.0% (CV). In addition, when haptoglobin values determined with the nephelometric assay were compared with hemoglobin-binding capacity determined by electrophoresis, the correlation coefficient was 0.968. The assay is essentially independent of phenotype and free of significant interference by hemolysis. The clinical correlation of haptoglobin values obtained for 100 selected patients with the nephelometric technique correlated well, if less than 250 mg/L, with the presence of hemolytic disease.

Effect of Blood Transfusion On Serum Haptoglobin.

Blood ◽  
2009 ◽  
Vol 114 (22) ◽  
pp. 3138-3138
Author(s):  
Shilpi Gupta ◽  
Fadi Nakhl ◽  
Kera Weiserbs ◽  
Frank J. Forte
Keyword(s):  
Conflicts Of Interest ◽  
Acute Phase Reactant ◽  
Normal Serum ◽  
Free Hemoglobin ◽  
Packed Red Blood Cells ◽  
Serum Haptoglobin ◽  
Haptoglobin Level ◽  
Larger Sample ◽  
Multiple Units ◽  
Stored Blood

Abstract Abstract 3138 Poster Board III-75 Introduction Haptoglobin, an alpha2 globulin, is an acute phase reactant, which functions to bind the globin portion of free hemoglobin in the blood. The half-life of serum haptoglobin is approximately five days, but in the presence of free hemoglobin, the hemoglobin-haptoglobin complex is rapidly cleared from the system causing a decrease in the measured serum haptoglobin (normal levels 36-195mg/dl). The measurement of serum haptoglobin is used as one of the laboratory markers for the diagnosis of hemolytic anemia. Since stored blood contains a variable amount of free hemoglobin, transfusion of this blood may affect haptoglobin levels and thereby negate the usefulness of haptoglobin measurement when hemolysis is suspected in recently transfused patients. Patients and methods Twenty-0ne patients, who were being transfused with more than one unit of packed red blood cells (PRBC), for non-hemolytic indications, were enrolled in the study and their serum haptoglobin levels were recorded before, immediately after, 24 hours after and 48 hours after PRBC transfusion. Observations and results A total of twenty-one patients were enrolled in the study and these patients received a total of forty-seven units of PRBC. The most common indication for transfusion was anemia secondary to blood loss. Eighteen patients received two units, one patient received three units, and two patients received four units of PRBC. Four patients received PRBC's that were more than 30 days old. When blood less than 30 days old was transfused there was a decrease to below normal in the haptoglobin level of only one patient, and this patient had a low normal serum haptoglobin level prior to transfusion. However, in four patients who received blood that was more than 30 days old, two (fifty percent) had a decrease in serum haptoglobin levels to below normal. Conclusion Serum haptoglobin can be used in the diagnosis of hemolysis in patients receiving multiple units of PRBC transfusions when the age of the transfused blood is less than thirty days. However, when PRBC's more than thirty days old are transfused measurement of serum haptoglobin might not prove to be a reliable indicator of hemolysis. Further studies with larger sample sizes are needed to confirm our findings. Disclosures No relevant conflicts of interest to declare.

scholarly journals Multivariate empirical mode decomposition–based structural damage localization using limited sensors

2021 ◽  
pp. 107754632110069
Author(s):  
Sandeep Sony ◽  
Ayan Sadhu
Keyword(s):  
Empirical Mode Decomposition ◽  
Structural Damage ◽  
Cost Effective ◽  
Threshold Value ◽  
Damage Localization ◽  
Time Frequency ◽  
Multivariate Empirical Mode Decomposition ◽  
Percentage Difference ◽  
Mode Decomposition

In this article, multivariate empirical mode decomposition is proposed for damage localization in structures using limited measurements. Multivariate empirical mode decomposition is first used to decompose the acceleration responses into their mono-component modal responses. The major contributing modal responses are then used to evaluate the modal energy for the respective modes. A damage localization feature is proposed by calculating the percentage difference in the modal energies of damaged and undamaged structures, followed by the determination of the threshold value of the feature. The feature of the specific sensor location exceeding the threshold value is finally used to identify the location of structural damage. The proposed method is validated using a suite of numerical and full-scale studies. The validation is further explored using various limited measurement cases for evaluating the feasibility of using a fewer number of sensors to enable cost-effective structural health monitoring. The results show the capability of the proposed method in identifying as minimal as 2% change in global modal parameters of structures, outperforming the existing time–frequency methods to delineate such minor global damage.

Resistance to Outflow of Cerebrospinal Fluid Determined by Bolus Injection Technique and Constant Rate Steady State Infusion in Humans

Neurosurgery ◽  
1985 ◽  
Vol 16 (3) ◽  
pp. 336-340 ◽  
Author(s):  
Michael Kosteljanetz
Keyword(s):  
Cerebrospinal Fluid ◽  
Steady State ◽  
Bolus Injection ◽  
Point Of View ◽  
Injection Technique ◽  
Communicating Hydrocephalus ◽  
Constant Rate ◽  
High Degree ◽  
Infusion Technique

Abstract Two methods for the determination of resistance to the outflow of cerebrospinal fluid, the bolus injection technique and the constant rate steady state infusion technique, were compared. Thirty-two patients with a variety of intracranial diseases (usually communicating hydrocephalus) were studied. There was a high degree of correlation between the resistance values obtained with the two methods, but values based on the bolus injection technique were systematically and statistically significantly lower than those obtained with the constant rate infusion test. From a practical point of view. both methods were found to be applicable in a clinical setting.

A Rapid Electrophoretic Method for the Determination of the Isoenzymes of Serum Lactate Dehydrogenase

1966 ◽  
Vol 12 (5) ◽  
pp. 308-313 ◽  
Author(s):  
Albert W Opher ◽  
Charles S Collier ◽  
Joseph M Miller
Keyword(s):  
Enzyme Activity ◽  
Lactate Dehydrogenase ◽  
Quantitative Determination ◽  
Serum Lactate ◽  
Serum Lactate Dehydrogenase ◽  
Electrophoretic Method ◽  
The Individual ◽  
Electrophoretic Procedure ◽  
Purple Formazan

Abstract A convenient electrophoretic procedure for the separation and quantitation of lactate dehydrogenase (LDH) isoenzymes is described. The system uses polyacetate Sepraphore III strips.* The areas of activity are shown by incubation with an LDH substrate combined with tetra-nitro-blue-tetrazolium. The reduction of the latter to the purple formazan is quantitatively related to the enzyme activity. Quantitative determination of the individual colored areas is performed by densitometry.

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