Fast determination of haptoglobin phenotype and calculation of hemoglobin binding capacity using high pressure gel permeation chromatography

2000 ◽  
Vol 291 (1) ◽  
pp. 43-51 ◽  
Author(s):  
J Delanghe ◽  
K Allcock ◽  
M Langlois ◽  
L Claeys ◽  
M De Buyzere
Keyword(s):  
High Pressure ◽  
Binding Capacity ◽  
Gel Permeation Chromatography ◽  
Fast Determination ◽  
Haptoglobin Phenotype ◽  
Hemoglobin Binding ◽  
Gel Permeation

Rapid analysis of bilirubin in neonatal serum. I. The binding of bilirubin to albumin.

1979 ◽  
Vol 25 (9) ◽  
pp. 1608-1612 ◽  
Author(s):  
K C Lu ◽  
K M Gooding ◽  
F E Regnier
Keyword(s):  
Serum Proteins ◽  
Binding Capacity ◽  
Gel Permeation Chromatography ◽  
Exchange Chromatography ◽  
Serum Samples ◽  
Binding Characteristics ◽  
Quenching Analysis ◽  
Gel Permeation ◽  
Bilirubin Binding

Abstract Evaulation of the severity of jaundice in the neonate may be determined by measuring the reserve binding capacity of serum proteins for free bilirubin. Determination of protein-bound bilirubin has been labor intensive, necessitating multiple runs on gel-permeation chromatography columns or, more recently, enzyme assays or fluorescence quenching analysis. We present a method for quantitation of free bilirubin and of bilirubin-binding capacity of serum by liquid chromatography. A gel-permeation column binds free bilirubin while allowing passage and quantitation of protein-bound bilirubin. Subsequent injection of a desorbing agent releases the adsorbed bilirubin from the column, permitting quantitation of free bilirubin. Bound and free serum bilirubin may be determined directly in less than 15 min using 10 microL of serum. The binding of bilirubin to neonatal serum is seen to be quite different from the binding to adult serum. Ion-exchange chromatography of adult and neonatal serum samples shows that their protein profiles are radically different. This difference probably accounts for the binding characteristics.

Determination of Sterigmatocystin in Corn and Oats by Gel Permeation and High-Pressure Liquid Chromatography

1976 ◽  
Vol 59 (5) ◽  
pp. 966-970
Author(s):  
Michael E Stack ◽  
Stanley Nesheim ◽  
Nathan L Brown ◽  
Albert E Pohland
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Coefficient Of Variation ◽  
Gel Permeation Chromatography ◽  
Automatic Instrument ◽  
Average Recovery ◽  
Ultraviolet Detector ◽  
Gel Permeation

Abstract Corn and oats samples are extracted with acetonitrile-water, followed by partition of the extract against hexane, transfer to chloroform, and elution from a silica gel column. The extract is purified by gel permeation chromatography on an automatic instrument. Reverse phase high-pressure liquid chromatography, using a 254 nm ultraviolet detector and 0.1M KH2PO4-acetonitrile (7+5) as the mobile phase, is used for quantitation. The average recovery from 6 samples of corn to which 0, 25, 50, and 100 μg sterigmatocystin/kg had been added was 59%, with a coefficient of variation of 8.4%. The average recovery from oats fortified at the same levels was 74%, with a coefficient of variation of 12%. A confirmation procedure based on hemiacctal derivative formation on a thin layer chromatographic plate is also described.

scholarly journals Molar mass determination of lignins and characterization of their polymeric structures by multi-detector gel permeation chromatography

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Evamaria C. Gaugler ◽  
Wolfgang Radke ◽  
Andrew P. Vogt ◽  
Dawn A. Smith
Keyword(s):  
Molar Mass ◽  
Lithium Bromide ◽  
Gel Permeation Chromatography ◽  
Mass Determination ◽  
Organosolv Lignin ◽  
Gel Permeation ◽  
Softwood Kraft Lignin ◽  
Polymeric Structures

AbstractMolar masses, Mark-Houwink-Sakurada (MHS) exponents, and refractive index increments (dn/dc) for three lignins were determined without derivatization by multi-detector gel permeation chromatography (GPC) in dimethylformamide (DMF) with 0.05 M lithium bromide (LiBr). The lack of effectiveness of fluorescence filters on molar mass determination by GPC-multi-angle laser light scattering (MALS) was confirmed for softwood kraft lignin (Indulin AT) and revealed for mixed hardwood organosolv lignin (Alcell) as well as soda straw/grass lignin (Protobind 1000). GPC with viscometry detection confirmed that these lignins were present as compact molecules. The MHS exponent α for Indulin AT and Alcell was in the order of 0.1. Additionally, the intrinsic viscosity of Protobind 1000 for a given molar mass was much lower than that of either Alcell or Indulin AT. This is the first report of dn/dc values for these three lignins in DMF with 0.05 M LiBr.

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