scholarly journals Possible Species Differences in RNA Metabolism of Lymph Node Cells

Blood ◽  
1965 ◽  
Vol 25 (6) ◽  
pp. 1009-1013 ◽  
Author(s):  
JOHN J. MILLER ◽  
JUDITH MITCHELL
Keyword(s):  
Lymph Node ◽  
Guinea Pigs ◽  
Species Differences ◽  
Plasma Cells ◽  
Rna Metabolism ◽  
Blast Cells ◽  
Lymph Node Cells

Abstract Differences in the rates of incorporation of tritiated uridine U-H3 of lymph node cells of different species have been found. Plasma cells from humans, guinea pigs, mice, and rabbits have a higher rate of incorporation of U-H3 than do plasma cells from rats, whereas human blast cells have a lower rate of U-H3 incorporation than blast cells from the various rodents.

scholarly journals Passive transfer of experimental autoimmune myasthenia by lymph node cells in inbred guinea pigs.

1975 ◽  
Vol 142 (3) ◽  
pp. 785-789 ◽  
Author(s):  
R Tarrab-Hazdi ◽  
A Aharonov ◽  
O Abramsky ◽  
I Yaar ◽  
S Fuchs
Keyword(s):  
Lymph Node ◽  
Human Disease ◽  
Guinea Pigs ◽  
Cellular Response ◽  
Clinical Signs ◽  
Clinical Manifestations ◽  
Passive Transfer ◽  
Lymph Node Cells ◽  
Autoimmune Myasthenia ◽  
Experimental Autoimmune

Passive transfer of experimental autoimmune myasthenia (EAM) was performed with lymph node cells from donor guinea pigs immunized with purified acetylcholine receptor (AChR) from Torpedo californica. Recipient animals revealed the same clinical signs and electromyographic patterns as observed in actively challenged animals. These phenomena are parallel to the clinical manifestations of the human disease myasthenia gravis, in which cellular response to AChR was recently demonstrated.

scholarly journals OCCURRENCE OF DELAYED HYPERSENSITIVITY DURING THE DEVELOPMENT OF ARTHUS TYPE HYPERSENSITIVITY

1958 ◽  
Vol 107 (1) ◽  
pp. 109-124 ◽  
Author(s):  
S. B. Salvin ◽  
Keyword(s):  
Lymph Node ◽  
Guinea Pig ◽  
Latent Period ◽  
Guinea Pigs ◽  
Specific Antigen ◽  
Egg Albumin ◽  
Lymph Node Cells ◽  
Type Inclusion ◽  
Antibody Determination ◽  
Circulating Antibody

Guinea pigs were injected in the footpads with either purified diphtheria toxoid or recrystallized egg albumin in Freund adjuvant without mycobacteria. Each guinea pig was then skin-tested only once with the specific antigen and bled for antibody determination. After injection of the sensitizing antigen, a latent period occurred during which neither sensitivity nor circulating antibody could be detected. A period of delayed sensitivity followed wherein circulating antibody could not be discerned and which could be transferred by lymph node cells. Ultimately, the Arthus type sensitivity developed, accompanied by circulating antibody. The duration and severity of reactions to homologous antigens during the last 2 phases varied with the antigen and with the dose. An increase in the sensitizing dose decreased the duration of the delayed type of allergy, a decrease in the dose prolonged the delayed type. Inclusion of mycobacterium in the sensitizing inoculum tended to introduce delayed sensitivity earlier and delay the onset of Arthus type sensitivity. When specific precipitate in antibody excess was included with the toxoid in the sensitizing dose, the onset of the Arthus phase was hastened. When lymph nodes from a large number of sensitized donors were removed during the latter part of the latent period, recipients of the cells showed a delayed type sensitivity.

Comparative immunostaining of T-associated plasma cells and other lymph-node cells in paraffin sections

1983 ◽  
Vol 43 (1) ◽  
pp. 31-36 ◽  
Author(s):  
Constantin S. Papadimitriou ◽  
Stella N. Stephanaki-Nikou ◽  
Vasiliki ◽  
D. Malamou-Mitsi
Keyword(s):  
Lymph Node ◽  
Plasma Cells ◽  
Paraffin Sections ◽  
Lymph Node Cells

scholarly journals THE TRANSFER OF LYMPH NODE CELLS IN THE STUDY OF THE IMMUNE RESPONSE TO FOREIGN PROTEINS

1955 ◽  
Vol 102 (4) ◽  
pp. 379-392 ◽  
Author(s):  
James C. Roberts ◽  
Frank J. Dixon
Keyword(s):  
Immune Response ◽  
Lymph Node ◽  
Plasma Cells ◽  
Primary Response ◽  
Secondary Response ◽  
Foreign Protein ◽  
Protein Antigens ◽  
Foreign Proteins ◽  
Lymph Node Cells ◽  
Wet Weight

A secondary immune response to the soluble foreign protein antigens I*BSA and I*BGG has been demonstrated when lymph node cells, largely lymphocytes with a few reticulo-endothelial and plasma cells, from previously immunized rabbits were transferred to x-radiated recipient rabbits, and the recipients then challenged with antigen. The total specific antibody synthesized by the transferred cells during the first 8 days of the secondary response amounted to approximately ⅔ of the wet weight of the transferred cells. In an attempt to elicit a primary response, lymph node cells were obtained from normal, non-immunized donors, and transferred to x-radiated recipients. No immune response was observed upon antigenic stimulation. When normal or previously immunized lymph node cells were incubated with antigen for periods up to 1 hour, washed and injected into recipients, no antibody production was observed.

scholarly journals SPECIFIC IMMUNE RESPONSE GENES OF THE GUINEA PIG

1971 ◽  
Vol 134 (6) ◽  
pp. 1538-1544 ◽  
Author(s):  
Harry G. Bluestein ◽  
Ira Green ◽  
Baruj Benacerraf
Keyword(s):  
Immune Response ◽  
Lymph Node ◽  
Guinea Pig ◽  
Guinea Pigs ◽  
Genetic Locus ◽  
Cytolytic Activity ◽  
Immune Response Gene ◽  
Response Gene ◽  
Large Measure ◽  
Lymph Node Cells

The lymph node cells from all L-glutamic acid and L-tyrosine (GT) responder random-bred guinea pigs were susceptible to lysis by strain 2 anti-strain 13 isoantisera in the presence of complement. These same antisera were cytolytic for lymph node cells of only some of the GT nonresponder animals. However, after absorption with cells, from a nonresponder guinea pig, susceptible to lysis, the anti-strain 13 antisera were no longer able to lyse cells from any GT nonresponder guinea pigs while retaining a large measure of their cytolytic activity for cells of all GT responder guinea pigs. Thus, at least two major strain 13 histocompatibility specificities are expressed on the cells of random-bred guinea pigs. The genetic locus controlling the expression of only one of those strain 13 histocompatibility specificities is linked to the GT immune response gene.

Demonstration of delayed hypersensitivity to Neisseria meningitidis

1973 ◽  
Vol 19 (12) ◽  
pp. 1473-1479 ◽  
Author(s):  
James F. Pribnow ◽  
Diana J. Besemer ◽  
Joan M. Hall ◽  
Neylan A. Vedros
Keyword(s):  
Cell Wall ◽  
Lymph Node ◽  
Neisseria Meningitidis ◽  
Guinea Pigs ◽  
Capillary Tube ◽  
Delayed Hypersensitivity ◽  
Somatic Antigen ◽  
Skin Reactions ◽  
Cell Wall Preparation ◽  
Lymph Node Cells

Guinea pigs were sensitized to Neisseria meningitidis group A by subcutaneous injection of viable meningococci. The animals were skin-tested with heat-killed N. meningitidis, a cell wall preparation of N. meningitidis, and a soluble somatic antigen prepared from the meningococci. Control skin-test substances included heat-killed Neisseria gonorrhoeae, heat-killed Escherichia coli, purified protein derivative, and Hank's balanced salt solution. Positive 24-h skin reactions, characterized by induration that measured greater than 5 × 5 mm (25 mm2), were produced only by heat-killed meningococci and with the cell wall preparations. The soluble somatic antigen produced only erythema. The meningococci also caused inhibition of migration of macrophages when peritoneal cells from the sensitized guinea pigs were used in the capillary tube MIF test. No inhibition of migration was produced with the control antigens. The delayed hypersensitivity was transferred with viable lymph node cells from the sensitized guinea pigs, but not with dead lymph node cells or with serum. The tests will be used to determine whether rabbits injected intravitreally with N. meningitidis develop delayed hypersensitivity.

scholarly journals LYMPHOCYTE PROLIFERATION IN VITRO INDUCED BY HAPTEN AUTOLOGOUS PROTEIN CONJUGATES

1974 ◽  
Vol 139 (3) ◽  
pp. 732-753 ◽  
Author(s):  
Bent Rubin ◽  
Hans Wigzell
Keyword(s):  
Lymph Node ◽  
T Lymphocytes ◽  
Dna Synthesis ◽  
Present Article ◽  
Immune Cells ◽  
Guinea Pigs ◽  
Differential Sensitivity ◽  
Specific Response ◽  
Lymph Node Cells

Immune lymph node cells from guinea pigs respond to soluble antigen in vitro by an increase in DNA synthesis. Optimal conditions for this proliferative response were studied in the present article. Under such conditions, immune cells showed increasing responses with increasing antigen concentration in vitro, the threshold dose of activation frequently being as low as 0.02 µg per culture. In contrast, normal lymph node cells (from FCA-stimulated animals) did only respond to antigen at very high doses (20 mg/culture), and immune cell dilution studies could be performed in normal cells without changing the kinetics of the antigen specific response of immune cells. Fractionation on anti-Ig columns indicated that purified, immune T lymphocytes were quite capable of proliferating in vitro upon antigen stimulation. However, our attempts to adsorb the proliferating cells onto chemically defined immunoadsorbants failed despite the fact that immune B cells (as measured by the rosette assay) were retained almost completely by such a procedure. Purified, immune T lymphocytes from guinea pigs immunized with different antigen concentrations in vivo and/or obtained at different times after immunization were tested for a differential sensitivity toward antigen-induced DNA synthesis in vitro. However, we were not able to demonstrate any regular increase in sensitivity to antigen in vitro, and if found, it seemed to be more dependent upon the number of antigen reactive cells in the population studied rather than upon differences in the average avidity of the receptors on the cells proliferating in vitro. The results in the present article are discussed in relation to current knowledge and hypotheses on T-lymphocyte receptors.

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