Brief Report: The Di Guglielmo Syndrome: Studies in Hemoglobin Synthesis

Abstract The in vitro synthesis of heme and globin has been studied in bone marrow cell suspensions obtained from five patients with Di Guglielmo syndrome. In all, a defect of heme synthesis was demonstrated, but globin synthesis was greatly reduced in only two of the five; in these two, the clinical course was a rapid one.

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Interleukin-1 (22-K factor) induces release of granulocyte-macrophage colony-stimulating activity from human mononuclear phagocytes

Blood ◽

10.1182/blood.v68.6.1316.1316 ◽

1986 ◽

Vol 68 (6) ◽

pp. 1316-1321 ◽

Cited By ~ 95

Author(s):

WE Fibbe ◽

J van Damme ◽

A Billiau ◽

PJ Voogt ◽

N Duinkerken ◽

...

Keyword(s):

Bone Marrow ◽

Bone Marrow Cell ◽

Marrow Cell ◽

Interleukin 1 ◽

Colony Growth ◽

Cell Suspensions ◽

Mononuclear Phagocytes ◽

Adherent Cells ◽

Macrophage Colony

Abstract An electrophoretically pure preparation of natural human interleukin-1 (IL-1) was shown to stimulate in vitro colony formation in human bone marrow cultures. Day 4 myeloid cluster-forming cells (CFC), as well as early (day 7) and late (day 10) granulocyte-macrophage colony-forming units (CFU-GM) were stimulated in a dose-dependent fashion. At optimal concentrations of IL-1, the number of day 4 CFC reached 72%, the number of day 7 CFU-GM reached 32%, and the number of day 10 CFU-GM reached 80% of the respective numbers of colonies obtained by addition of crude leukocyte-conditioned medium (LCM). The IL-1-induced stimulatory effect on CFU-GM growth could be completely neutralized by a rabbit anti-IL-1 antiserum. Colony growth was abrogated by depleting the marrow cell suspensions of phagocytic cells prior to IL-1 addition. Conversely, the effect could be reintroduced by addition of marrow-derived adherent cells to bone marrow cell suspensions that had been depleted of both phagocytic and E rosetting T cells. Furthermore, media conditioned by bone marrow-derived adherent cells or by peripheral blood mononuclear phagocytes in the presence but not in the absence of IL-1, stimulated in vitro colony growth of phagocyte-depleted bone marrow cell suspensions. These results indicate that IL-1 induces release of granulocyte-macrophage colony-stimulating activity (GM-CSA) from human mononuclear phagocytes.

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Interleukin-1 (22-K factor) induces release of granulocyte-macrophage colony-stimulating activity from human mononuclear phagocytes

Blood ◽

10.1182/blood.v68.6.1316.bloodjournal6861316 ◽

1986 ◽

Vol 68 (6) ◽

pp. 1316-1321

Author(s):

WE Fibbe ◽

J van Damme ◽

A Billiau ◽

PJ Voogt ◽

N Duinkerken ◽

...

Keyword(s):

Bone Marrow ◽

Bone Marrow Cell ◽

Marrow Cell ◽

Interleukin 1 ◽

Colony Growth ◽

Cell Suspensions ◽

Mononuclear Phagocytes ◽

Adherent Cells ◽

Macrophage Colony

An electrophoretically pure preparation of natural human interleukin-1 (IL-1) was shown to stimulate in vitro colony formation in human bone marrow cultures. Day 4 myeloid cluster-forming cells (CFC), as well as early (day 7) and late (day 10) granulocyte-macrophage colony-forming units (CFU-GM) were stimulated in a dose-dependent fashion. At optimal concentrations of IL-1, the number of day 4 CFC reached 72%, the number of day 7 CFU-GM reached 32%, and the number of day 10 CFU-GM reached 80% of the respective numbers of colonies obtained by addition of crude leukocyte-conditioned medium (LCM). The IL-1-induced stimulatory effect on CFU-GM growth could be completely neutralized by a rabbit anti-IL-1 antiserum. Colony growth was abrogated by depleting the marrow cell suspensions of phagocytic cells prior to IL-1 addition. Conversely, the effect could be reintroduced by addition of marrow-derived adherent cells to bone marrow cell suspensions that had been depleted of both phagocytic and E rosetting T cells. Furthermore, media conditioned by bone marrow-derived adherent cells or by peripheral blood mononuclear phagocytes in the presence but not in the absence of IL-1, stimulated in vitro colony growth of phagocyte-depleted bone marrow cell suspensions. These results indicate that IL-1 induces release of granulocyte-macrophage colony-stimulating activity (GM-CSA) from human mononuclear phagocytes.

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INCORPORATION IN VITRO OF LABELED AMINO ACIDS INTO BONE MARROW CELL PROTEINS

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)56315-8 ◽

1950 ◽

Vol 186 (1) ◽

pp. 297-307 ◽

Cited By ~ 8

Author(s):

Henry. Borsook ◽

Clara L. Deasy ◽

A.J. Haagen-Smit ◽

Geoffrey. Keighley ◽

Peter H. Lowy

Keyword(s):

Amino Acids ◽

Bone Marrow ◽

Bone Marrow Cell ◽

Marrow Cell

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Thy 1.2+ Leukemia Cells Eradicated From In Vitro Leukemia-Bone Marrow Cell Mixtures by Antibody-Toxin Conjugates2

JNCI Journal of the National Cancer Institute ◽

10.1093/jnci/72.5.1095 ◽

1984 ◽

Keyword(s):

Bone Marrow ◽

Bone Marrow Cell ◽

Marrow Cell ◽

Leukemia Cells

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Effect of Erythropoietin on RNA Synthesis by Normal and Leukemic Bone Marrow and Spleen Cell Suspensions In Vitro

Blood ◽

10.1182/blood.v44.4.535.535 ◽

1974 ◽

Vol 44 (4) ◽

pp. 535-542 ◽

Cited By ~ 5

Author(s):

Evelyn E. Handler ◽

Naomi Mendelsohn ◽

Eugene S. Handler

Keyword(s):

Bone Marrow ◽

Rna Synthesis ◽

Bone Marrow Cells ◽

Marrow Cell ◽

Cell Suspensions ◽

Myelogenous Leukemia ◽

Leukemic Cells ◽

Spleen Cells ◽

Normal Bone Marrow Cell

Abstract Erythropoietin (EPO) induced a 42% increase in 3H-uridine incorporation into RNA after a 5-hr culture of normal bone marrow cell suspensions. Bone marrow cells obtained from rats 3-5 days after the initiation of a myelogenous leukemia exhibited a decreased responsivity to EPO. At this time incorporation of the isotope into RNA in the presence of EPO was approximately 50% of controls. Rats rendered leukemic 8-10 days prior to culture showed no bone marrow response to EPO even in those instances where leukemic cells comprised a relatively small percentage of the marrow compartment. EPO had little or no effect on RNA synthesis by spleen cells obtained from normal and leukemic rats. This was noted even in those leukemic spleens in which erythropoiesis was observed. The data suggest that the anemia associated with myelogenous leukemia may, in part, be due to a loss of EPO-responsive cells and/or a loss of sensitivity of these elements to normal humoral control.

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