scholarly journals Effects of Hydrocortisone on the Yield and Bactericidal Function of Granulocytes Collected by Continuous-flow Centrifugation

Blood ◽  
1974 ◽  
Vol 44 (3) ◽  
pp. 435-443 ◽  
Author(s):  
Mamoru Shoji ◽  
William R. Vogler
Keyword(s):  
Single Dose ◽  
Flow Rate ◽  
Continuous Flow ◽  
Treated Group ◽  
Flow Cell ◽  
Controlled Study ◽  
Single Intravenous Injection ◽  
Cell Separator ◽  
Bactericidal Function

Abstract The usefulness of granulocyte transfusions is in part dependent upon the number of granulocytes transfused. The invention of the continuous-flow cell separator has made it possible to obtain granulocytes from normal donors. Efforts to improve the yield are under study. This controlled study was undertaken to determine the effect of a single dose of hydrocortisone on granulocyte yield from volunteer donors and on granulocyte bactericidal function. Twenty-two normal volunteers were randomized between no therapy or a single intravenous injection of 120 mg/sq m of hydrocortisone 2 hr prior to initiation of a 4-hr leukapheresis using the Aminco cell separator operated at 750 rpm and a flow rate of 41 ml/min. Significant increases in granulocyte yield and reductions in lymphocyte and monocyte yields were obtained in the hydrocortisone-treated group. Granulocytes from each group were equally effective in the phagocytosis of yeast particles and in vitro bactericidal activity.

Apheresis experience with a continuous flow cell separator

Transfusion ◽  
1988 ◽  
Vol 28 (5) ◽  
pp. 470-473 ◽  
Author(s):  
F Norol ◽  
C Aubert ◽  
F Scotto ◽  
N Duedari
Keyword(s):  
Continuous Flow ◽  
Flow Cell ◽  
Cell Separator

Concentration of Progenitor Cells Collected from Bone Marrow Fluid Using a Continuous Flow Cell Separator

2001 ◽  
Vol 5 (1) ◽  
pp. 46-48 ◽  
Author(s):  
Kazuo Tsubaki ◽  
Takeshi Ariyama ◽  
Toshihisa Ueno ◽  
Yukito Kogi ◽  
Shiho Itoh ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Progenitor Cells ◽  
Continuous Flow ◽  
Flow Cell ◽  
Cell Separator

In vivo-in vitro correlations of salicylate saliva levels and continuous flow cell dissolution rates

1983 ◽  
Vol 15 (2) ◽  
pp. 167-175 ◽  
Author(s):  
Hartmut Derendorf ◽  
Gertrude Drehsen ◽  
Peter Rohdewald
Keyword(s):  
Continuous Flow ◽  
Flow Cell ◽  
Dissolution Rates

Continuous flow cell separator use for bone marrow processing

1991 ◽  
Vol 12 (3) ◽  
pp. 183-187 ◽  
Author(s):  
P.K. Baker ◽  
E.G.H. Rhodes ◽  
J.K.M. Duguid
Keyword(s):  
Bone Marrow ◽  
Continuous Flow ◽  
Flow Cell ◽  
Cell Separator

Plateletapheresis with “Haemonetics 30” through a new three-way reinfusion set®

1987 ◽  
Vol 10 (1) ◽  
pp. 57-60
Author(s):  
S. Pennisi ◽  
E. Del Fabro
Keyword(s):  
Extracorporeal Circulation ◽  
Continuous Flow ◽  
Flow Cell ◽  
Discontinuous Flow ◽  
Filling Stage ◽  
Cell Separator ◽  
Set Up

The main disadvantages of the discontinuous flow cell separator Haemonetics 30 (Haemonetics Corp. Natick, Mass.) compared to continuous flow separators are that there is a longer course procedure and a larger amount of donor's blood in extracorporeal circulation. These drawbacks can be reduced by means of a simple technical set-up. Since plasma reinfusion may commence as soon as the bowl filling stage is completed, the amount of blood in extracorporeal circulation can be greatly reduced simply by substituting a three-way setfor the two-way reinfusion set (BT 293).

scholarly journals Distribution of selenium in sheep treated with dipheny diselenide

2018 ◽  
Vol 70 (4) ◽  
pp. 1017-1022
Author(s):  
M.L.R. Leal ◽  
J.B.T. Rocha ◽  
C.L.D. Corte ◽  
A.R. Aires ◽  
J.F.X. Rocha ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Single Dose ◽  
Intravenous Injection ◽  
Selenium Concentration ◽  
Treated Group ◽  
Post Treatment ◽  
Control Group ◽  
Diphenyl Diselenide ◽  
Single Intravenous Injection

ABSTRACT The aim of the present study was to report the in vivo distribution of selenium in sheep. For this, animals were allocated into two groups (control group and treated group) and kept in metabolic cages for a period of 37 days. The treated group received a single dose (6µmol/kg) of Diphenyl Diselenide, intravenously. Plasma and erythrocytes samples were collected at different times. Adipose tissue, muscles (latissimusdorsi, semitendinosus, and supra-scapular) heart, liver, lung, kidney, intestine and brain were sampled at 30 days post-treatment, in order to determine the selenium concentration. The results demonstrated that the selenium, from the Diphenyl Diselenide group, was higher in erythrocytes (4.8mg/L, six hours post-treatment) when compared with the control sheep. The deposition of selenium occurred in the liver (7.01µg/g), brain (3.53µg/g) and kidney (2.02µg/g). After 30 days of a single intravenous injection of Diphenyl Diselenide, liver was the main organ of selenium deposition.

Large-scale plasmapheresis in equines adapting an IBM continuous-flow cell separator

1987 ◽  
Vol 8 (2) ◽  
pp. 177-183
Author(s):  
Cyrus Poonawalla ◽  
A.C. Namjoshi
Keyword(s):  
Continuous Flow ◽  
Large Scale ◽  
Flow Cell ◽  
Cell Separator
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