scholarly journals The coexistence of acute myeloblastic leukemia and diffuse histiocytic lymphoma in the same patient as demonstrated by multiparameter analysis

Blood ◽  
1979 ◽  
Vol 54 (6) ◽  
pp. 1428-1433
Author(s):  
DJ Straus ◽  
M Andreeff ◽  
HJ Hansen ◽  
R Mertelsmann ◽  
B Koziner ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Flow Cytometry ◽  
Lymph Node ◽  
Dna Content ◽  
Acute Myeloblastic Leukemia ◽  
Histiocytic Lymphoma ◽  
Cellular Dna ◽  
Multiparameter Analysis ◽  
Diploid Cells ◽  
Diffuse Histiocytic Lymphoma

Measurement of cellular DNA content by flow cytometry demonstrated presence of two distinct aneuploid neoplasms in a patient who developed acute myeloblastic leukemia (AML) 4 mo after diagnosis of a diffuse histiocytic lymphoma (DHL). A lymph node aspirate contained peroxidase- negative, “null,” hyperdiploid (2.6C) DHL cells, while the bone marrow (BM) contained 84% primitive peroxidase-positive tetraploid AML cells (4.0C). Minor populations of hyperdiploid HDL and normal diploid cells could be detected by flow-cytometry in the BM, and all three populations were also seen in the peripheral blood.

scholarly journals The coexistence of acute myeloblastic leukemia and diffuse histiocytic lymphoma in the same patient as demonstrated by multiparameter analysis

Blood ◽  
1979 ◽  
Vol 54 (6) ◽  
pp. 1428-1433 ◽  
Author(s):  
DJ Straus ◽  
M Andreeff ◽  
HJ Hansen ◽  
R Mertelsmann ◽  
B Koziner ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Flow Cytometry ◽  
Lymph Node ◽  
Dna Content ◽  
Acute Myeloblastic Leukemia ◽  
Histiocytic Lymphoma ◽  
Cellular Dna ◽  
Multiparameter Analysis ◽  
Diploid Cells ◽  
Diffuse Histiocytic Lymphoma

Abstract Measurement of cellular DNA content by flow cytometry demonstrated presence of two distinct aneuploid neoplasms in a patient who developed acute myeloblastic leukemia (AML) 4 mo after diagnosis of a diffuse histiocytic lymphoma (DHL). A lymph node aspirate contained peroxidase- negative, “null,” hyperdiploid (2.6C) DHL cells, while the bone marrow (BM) contained 84% primitive peroxidase-positive tetraploid AML cells (4.0C). Minor populations of hyperdiploid HDL and normal diploid cells could be detected by flow-cytometry in the BM, and all three populations were also seen in the peripheral blood.

Flow cytometry, cellular DNA content, and prognosis in human malignancy.

1987 ◽  
Vol 5 (10) ◽  
pp. 1690-1703 ◽  
Author(s):  
D E Merkel ◽  
L G Dressler ◽  
W L McGuire
Keyword(s):  
Flow Cytometry ◽  
Data Base ◽  
Dna Content ◽  
Dna Analysis ◽  
Clinical Decision Making ◽  
Risk Groups ◽  
Clinical Decision ◽  
Tumor Type ◽  
Cellular Dna ◽  
Bladder Carcinomas

The use of flow cytometry to analyze the cellular DNA content of human malignancies has become increasingly commonplace. The relationship between abnormalities in DNA content or proliferative characteristics and prognosis is becoming clear for a variety of malignancies in part through new techniques that permit analysis of archival material. High- and low-risk groups of patients with early breast and bladder carcinomas, non-small-cell lung cancer, and colorectal, ovarian, and cervical carcinoma can be distinguished on the basis of abnormal stemline DNA content. In several hematologic and common pediatric malignancies, the prognostic relevance of DNA content flow cytometry has been similarly established. Though the interpretation of tumor cell cycle analyses is less certain, this characteristic may also be prognostically important. However, generalizations cannot be made when applying flow cytometric DNA analysis to clinical decision making. The prognostic importance of an abnormal DNA histogram for an individual patient must be assessed on the basis of the relevant data base for that particular tumor type. The current extent of this data base for various malignancies is reviewed.

scholarly journals Polyploid Formation via Chromosome Duplication Induced by CTP:Phosphocholine Cytidylyltransferase Deficiency and Bcl-2 Overexpression: Identification of Two Novel Endogenous Factors

2005 ◽  
Vol 53 (6) ◽  
pp. 725-733 ◽  
Author(s):  
You-Jun Shen ◽  
Cynthia J. DeLong ◽  
Francois Tercé ◽  
Timothy Kute ◽  
Mark C. Willingham ◽  
...  
Keyword(s):  
Dna Content ◽  
Chinese Hamster ◽  
B Cell Lymphoma ◽  
Negative Regulator ◽  
Ovary Cell ◽  
Endogenous Factors ◽  
Ctp:Phosphocholine Cytidylyltransferase ◽  
Polyploid Formation ◽  
Cellular Dna ◽  
Diploid Cells

Polyploidy is a profound phenotype found in tumors and its mechanism is unknown. We report here that when B-cell lymphoma gene-2 (Bcl-2) was overexpressed in a Chinese hamster ovary cell line that was deficient in CTP:phosphocholine cytidylyltransferase (CT), cellular DNA content doubled. The higher DNA content was due to a permanent conversion from diploid cells to tetraploid cells. The mechanism of polyploid formation could be attributed to the duplication of 18 parental chromosomes. The rate of conversion from diploid to tetraploid was Bcl-2 dose dependent. The diploid genome was not affected by Bcl-2 expression or by CT deficiency alone. Endogenous CT or expression of recombinant rat liver CTα prior to Bcl-2 expression prevented the formation of polyploid cells. This conversion was irreversible even when both initiating factors were removed. In this study, we have identified Bcl-2 as a positive regulator and CTα as a negative regulator of polyploid formation.

scholarly journals Method for analysis of cellular DNA content of paraffin-embedded pathological material using flow cytometry.

1983 ◽  
Vol 31 (11) ◽  
pp. 1333-1335 ◽  
Author(s):  
D W Hedley ◽  
M L Friedlander ◽  
I W Taylor ◽  
C A Rugg ◽  
E A Musgrove
Keyword(s):  
Flow Cytometry ◽  
Retrospective Study ◽  
Clinical Outcome ◽  
Dna Content ◽  
Prognostic Significance ◽  
Distilled Water ◽  
Archival Material ◽  
Cellular Dna ◽  
Pathological Material ◽  
Dna Histograms

A method has been developed that allows flow cytometry to be used for measuring the cellular DNA content of paraffin-embedded human tumors. Thick (i.e., 30 micron) sections were cut from tissue blocks using a microtome and dewaxed in xylene. The sections were then rehydrated by sequentially immersing them in 100, 95, 70, and 50% ethanol before finally washing in distilled water. Single cell suspensions were then prepared by incubation in 0.5% pepsin, pH 1.5, at 37 degrees C for 30 min. The cells were counted, washed, and stained with 1 microgram/ml 4',6'-diamidino-2-phenylindole for 30 min, and DNA content was measured using an ICP 22 flow cytometer. There was a good correlation between the DNA histograms produced using this method and those obtained using unfixed tissue from the same tumor stained with ethidium bromide plus mithramycin. This method allows the retrospective study of archival material where the clinical outcome is already known, and it should, therefore, be particularly useful for determining the prognostic significance of abnormal DNA content measured by flow cytometry.

Analysis of Cellular DNA Content by Flow Cytometry

2017 ◽  
Vol 82 (1) ◽  
Author(s):  
Zbigniew Darzynkiewicz ◽  
Xuan Huang ◽  
Hong Zhao
Keyword(s):  
Flow Cytometry ◽  
Dna Content ◽  
Cellular Dna

scholarly journals Flow cytometry of DNA content in human bone marrow: a critical reappraisal

Blood ◽  
1980 ◽  
Vol 55 (5) ◽  
pp. 734-740
Author(s):  
GM Dosik ◽  
B Barlogie ◽  
W Gohde ◽  
D Johnston ◽  
JL Tekell ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Flow Cytometry ◽  
Dna Content ◽  
Bone Marrow Aspirate ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Blood Contamination ◽  
Dna Distribution ◽  
M Phase ◽  
Normal Human

Because cytokinetic studies of the human bone marrow aspirate as a prognostic factor and as a monitor of drug perturbation are frequently inconsistent, we investigated reproducibility of DNA distribution measured by flow cytometry of DNA content in patients with morphologically normal bone marrow. In 15 patients, correlation was noted between DNA distributions simultaneously obtained on right and left iliac crest bone marrow aspirates (r = .588), although considerable variation in individuals was encountered. Much better reproducibility (r = .879) was achieved using bilateral core biopsy of bone marrow in these same patients. In 60 samples, comparison of DNA distribution between bone marrow aspirate and simultaneously obtained biopsy revealed higher relative proportions of S and G2 + M phase cells in biopsies (p less than 0.001), suggesting peripheral blood contamination of aspirate material. Brisk shaking of biopsy specimens in saline expelled a representative sample in the supernatant that could be subjected to simultaneous cytomorphological and cytokinetic analysis. To improve reproducibility of DNA content determinations in normal human bone marrow, bone marrow biopsy should be utilized.

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