Pluripotent hemopoietic progenitors (CFU-GEMM) in polycythemia vera: analysis of erythropoietin requirement and proliferative activity

Pluripotent hemopoietic progenitors (CFU-GEMM) give rise to multilineage hemopoietic colonies in culture. We have examined the erythropoietin requirements of CFU-GEMM-derived erythroid progeny in patients with polycythemia vera (PV) and studied their proliferative activity by short-term exposure to 3HTdR. Mixed colonies with erythroid components were observed in all bone marrow and peripheral blood samples from patients with PV that were cultured without addition of exogenous erythropoietin. This response is consistent with previously reported growth patterns for CFU-E and BFU-E. The frequency of mixed colonies increased regularly when erythropoietin was added to the cultures. Short-term exposure of peripheral blood specimens to 3HTdR prior to plating yielded a reduction of the plating efficiency by 20%- 70% when compared to cells that were not exposed to 3HTdR. The observation of cycling CFU-GEMM in PV contrasts with the usually quiescent behavior of CFU-GEMM in peripheral blood of normal individuals under steady-state conditions. These results support the view that the increased proliferative rate observed for CFU-GEMM may be responsible for the increased formation of blood cells in PV.

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Pluripotent hemopoietic progenitors (CFU-GEMM) in polycythemia vera: analysis of erythropoietin requirement and proliferative activity

Blood ◽

10.1182/blood.v58.6.1224.1224 ◽

1981 ◽

Vol 58 (6) ◽

pp. 1224-1227 ◽

Cited By ~ 13

Author(s):

AA Fauser ◽

HA Messner

Keyword(s):

Polycythemia Vera ◽

Peripheral Blood ◽

Blood Cells ◽

Proliferative Activity ◽

Growth Patterns ◽

Short Term ◽

Proliferative Rate ◽

Normal Individuals ◽

Short Term Exposure ◽

Blood Specimens

Abstract Pluripotent hemopoietic progenitors (CFU-GEMM) give rise to multilineage hemopoietic colonies in culture. We have examined the erythropoietin requirements of CFU-GEMM-derived erythroid progeny in patients with polycythemia vera (PV) and studied their proliferative activity by short-term exposure to 3HTdR. Mixed colonies with erythroid components were observed in all bone marrow and peripheral blood samples from patients with PV that were cultured without addition of exogenous erythropoietin. This response is consistent with previously reported growth patterns for CFU-E and BFU-E. The frequency of mixed colonies increased regularly when erythropoietin was added to the cultures. Short-term exposure of peripheral blood specimens to 3HTdR prior to plating yielded a reduction of the plating efficiency by 20%- 70% when compared to cells that were not exposed to 3HTdR. The observation of cycling CFU-GEMM in PV contrasts with the usually quiescent behavior of CFU-GEMM in peripheral blood of normal individuals under steady-state conditions. These results support the view that the increased proliferative rate observed for CFU-GEMM may be responsible for the increased formation of blood cells in PV.

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Gene Expression Analysis in Human Peripheral Blood Cells after 900 MHz RF-EMF Short-Term Exposure

Radiation Research ◽

10.1667/rr14909.1 ◽

2018 ◽

Vol 189 (5) ◽

pp. 529-540 ◽

Cited By ~ 4

Author(s):

Andreas Lamkowski ◽

Matthias Kreitlow ◽

Jörg Radunz ◽

Martin Willenbockel ◽

Frank Sabath ◽

...

Keyword(s):

Gene Expression ◽

Expression Analysis ◽

Peripheral Blood ◽

Blood Cells ◽

Gene Expression Analysis ◽

Human Peripheral Blood ◽

Peripheral Blood Cells ◽

Short Term ◽

Short Term Exposure

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Genotoxicity assessment of fipronil (frontline plus®) in Canis familiaris

Pesquisa Veterinária Brasileira ◽

10.1590/s0100-736x2017000300009 ◽

2017 ◽

Vol 37 (3) ◽

pp. 257-260

Author(s):

Liane Ziliotto ◽

Stelio P.L. Luna ◽

Daniel A. A.Filho ◽

Ludimila O. Resende ◽

Aline G. Aun ◽

...

Keyword(s):

Peripheral Blood ◽

Blood Cells ◽

Canis Familiaris ◽

Neck Region ◽

Domestic Animals ◽

Genotoxic Effect ◽

Single Exposure ◽

Short Term ◽

Short Term Exposure ◽

First Time

ABSTRACT: Fipronil is a pesticide widely used for controlling fleas and ticks in domestic animals, and its short-term exposure can lead to serious effects on animals. However, the possible genotoxic effect of this compound has not been investigated in target animals. Based on the hypothesis that fipronil can induce genotoxicity, this study evaluated the effect of fipronil on DNA damage in peripheral blood cells. For that purpose, ten dogs of both sexes were used in the study. The product (6.7mg/kg) was applied on the dorsal neck region of each animal. Peripheral blood samples were collected immediately prior to application of the product, and at 3, 8 and 24 hours after the application. Samples were processed for comet assay. No statistically significant differences were found among the four time points. The current study suggests for the first time that a single exposure to this pesticide does not induce systemic genotoxic effect in dogs.

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Peripheral blood blast cell progenitors in human preleukemia

Blood ◽

10.1182/blood.v59.1.106.106 ◽

1982 ◽

Vol 59 (1) ◽

pp. 106-109 ◽

Cited By ~ 12

Author(s):

JS Senn ◽

HA Messner ◽

PH Pinkerton ◽

L Chang ◽

B Nitsch ◽

...

Keyword(s):

Acute Leukemia ◽

Clinical Significance ◽

Peripheral Blood ◽

Tritiated Thymidine ◽

Blast Cell ◽

Short Term ◽

Short Term Exposure

Abstract Progenitors of blast cell colonies have been identified in acute leukemia. The peripheral blood of 18 of 25 patients with preleukemic states yielded low numbers of blast cell colonies, and the colony- forming cells were in an active proliferative state when assessed using short-term exposure to tritiated thymidine. The clinical significance of blast cell colonies is uncertain, but we suggest that further analysis of this cultural abnormality may lead to a better understanding of mechanisms and management in preleukemia.

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Impact of Short-Term Systemic Hypoxia on Phagocytosis, Cytokine Production, and Transcription Factor Activation in Peripheral Blood Cells

Mediators of Inflammation ◽

10.1155/2011/429501 ◽

2011 ◽

Vol 2011 ◽

pp. 1-9 ◽

Cited By ~ 16

Author(s):

Michael Fritzenwanger ◽

Christian Jung ◽

Bjoern Goebel ◽

Alexander Lauten ◽

Hans R. Figulla

Keyword(s):

Peripheral Blood ◽

Blood Cells ◽

Cytokine Production ◽

Interferon Γ ◽

Peripheral Blood Cells ◽

Immune Functions ◽

Cellular Functions ◽

Short Term ◽

Transcription Factor Activation ◽

Systemic Hypoxia

Hypoxia frequently associated with certain physiologic and pathologic conditions influences numerous cellular functions. Because the effects of short-term hypoxia are incompletely understood, we examined phagocytosis and cytokine production as well as the activation of the transcription factors HIF-1 and NFκB in peripheral blood cells of healthy volunteers exposed to an oxygen concentration equivalent to that found at a height of 5500 m. Furthermore, we analysed plasma HIF-1 and serum concentrations of various HIF-1-dependent genes. Results showed that short-term hypoxia increased phagocytosis in neutrophils without affecting monocyte phagocytosis. Hypoxia decreased basal TNFα concentration in monocytes and basal interferon γ concentration in CD4+T lymphocytes. In contrast, plasma HIF and serum VEGF concentrations were not affected by hypoxia, although serum EPO concentration was raised. In PBMC, hypoxia increased cytosolic HIF-1 concentration without affecting nuclear HIF-1 concentration and led to a rise in the nuclear NFκB in PBMC. Our results show that short-term hypoxia affects immune functions in healthy individuals. Furthermore, we speculate that the effects of hypoxia are not due to HIF-1, but are caused by the activation of NFκB .

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Individual BFU-E in polycythemia vera produce both erythropoietin dependent and independent progeny

Blood ◽

10.1182/blood.v61.5.876.876 ◽

1983 ◽

Vol 61 (5) ◽

pp. 876-884 ◽

Cited By ~ 26

Author(s):

J Cashman ◽

D Henkelman ◽

K Humphries ◽

C Eaves ◽

A Eaves

Keyword(s):

Polycythemia Vera ◽

Peripheral Blood ◽

Small Volume ◽

Early Stage ◽

Final Concentration ◽

The Other ◽

Normal Individuals ◽

Additional Control ◽

Minor Population ◽

A Minor

Abstract Erythropoietic progenitors from peripheral blood of normal individuals or patients with polycythemia vera (PV) were cultured in methylcellulose medium containing 2.5 U/ml of erythropoietin (Ep). After 7–9 days, colonies considered to be early stage large bursts were individually removed, resuspended in a small volume of fresh methylcellulose medium, and then divided between 2 dishes. To one of these secondary cultures, sufficient Ep was added to bring the concentration of Ep up to approximately 3 U/ml. To the other was added an equal volume of medium but no Ep. The final concentration of Ep in these cultures was determined to be less than 0.01 U/ml. Nine days later, both types of secondary cultures were scored for the presence of colonies containing 8 or more hemoglobinized erythroblasts. Of 90 primary colonies from 3 normal individuals assessed in this way, 59 gave secondary erythroid colonies in the high Ep cultures, while none gave secondary erythroid colonies in the low Ep cultures. Additional control experiments in which primary colonies from normal individuals were divided into duplicate high Ep cultures showed that on average, the procedure used divided primary colonies equally. Of 109 primary colonies from 5 PV patients that yielded secondary erythroid colonies in the high Ep cultures, 21 yielded no secondary erythroid colonies in the low Ep cultures. The other 88 yielded erythroid colonies in both, but the secondary colonies in the low Ep cultures were consistently smaller in size and significantly fewer in number. Similar results were obtained when primary colonies were generated in cultures to which no Ep was added. These findings indicate that primitive BFU-E in patients with PV can be subdivided into 2 populations: a minor population restricted to the production of erythroid colony-forming cells (Ep- dependent progenitors) that require Ep for their detection, and a major population that is not restricted in this way. In addition, these experiments show that most of the primitive BFU-E that generate Ep- independent progenitors also produce significant numbers of cells that are Ep-dependent.

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Telomeric length and telomerase activity vary with age in peripheral blood cells obtained from normal individuals

Human Genetics ◽

10.1007/s004390050711 ◽

1998 ◽

Vol 102 (4) ◽

pp. 397-402 ◽

Cited By ~ 189

Author(s):

Hiroshi Iwama ◽

K. Ohyashiki ◽

Junko H. Ohyashiki ◽

Shigifumi Hayashi ◽

Naoyuki Yahata ◽

...

Keyword(s):

Peripheral Blood ◽

Blood Cells ◽

Telomerase Activity ◽

Peripheral Blood Cells ◽

Normal Individuals ◽

Telomeric Length

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Individual BFU-E in polycythemia vera produce both erythropoietin dependent and independent progeny

Blood ◽

10.1182/blood.v61.5.876.bloodjournal615876 ◽

1983 ◽

Vol 61 (5) ◽

pp. 876-884 ◽

Cited By ~ 1

Author(s):

J Cashman ◽

D Henkelman ◽

K Humphries ◽

C Eaves ◽

A Eaves

Keyword(s):

Polycythemia Vera ◽

Peripheral Blood ◽

Small Volume ◽

Early Stage ◽

Final Concentration ◽

The Other ◽

Normal Individuals ◽

Additional Control ◽

Minor Population ◽

A Minor

Erythropoietic progenitors from peripheral blood of normal individuals or patients with polycythemia vera (PV) were cultured in methylcellulose medium containing 2.5 U/ml of erythropoietin (Ep). After 7–9 days, colonies considered to be early stage large bursts were individually removed, resuspended in a small volume of fresh methylcellulose medium, and then divided between 2 dishes. To one of these secondary cultures, sufficient Ep was added to bring the concentration of Ep up to approximately 3 U/ml. To the other was added an equal volume of medium but no Ep. The final concentration of Ep in these cultures was determined to be less than 0.01 U/ml. Nine days later, both types of secondary cultures were scored for the presence of colonies containing 8 or more hemoglobinized erythroblasts. Of 90 primary colonies from 3 normal individuals assessed in this way, 59 gave secondary erythroid colonies in the high Ep cultures, while none gave secondary erythroid colonies in the low Ep cultures. Additional control experiments in which primary colonies from normal individuals were divided into duplicate high Ep cultures showed that on average, the procedure used divided primary colonies equally. Of 109 primary colonies from 5 PV patients that yielded secondary erythroid colonies in the high Ep cultures, 21 yielded no secondary erythroid colonies in the low Ep cultures. The other 88 yielded erythroid colonies in both, but the secondary colonies in the low Ep cultures were consistently smaller in size and significantly fewer in number. Similar results were obtained when primary colonies were generated in cultures to which no Ep was added. These findings indicate that primitive BFU-E in patients with PV can be subdivided into 2 populations: a minor population restricted to the production of erythroid colony-forming cells (Ep- dependent progenitors) that require Ep for their detection, and a major population that is not restricted in this way. In addition, these experiments show that most of the primitive BFU-E that generate Ep- independent progenitors also produce significant numbers of cells that are Ep-dependent.

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Growth of Leukemic Cells in Culture

Blood ◽

10.1182/blood.v40.6.806.806 ◽

1972 ◽

Vol 40 (6) ◽

pp. 806-811 ◽

Cited By ~ 18

Author(s):

M. T. Aye ◽

J. E. Till ◽

E. A. McCulloch

Keyword(s):

Conditioned Medium ◽

Peripheral Blood ◽

Blood Cells ◽

Leukemic Cells ◽

Peripheral Blood Cells ◽

Leukemic Blast ◽

Short Term ◽

New Approach ◽

Cells In Culture ◽

Blast Cells

Abstract Peripheral blood cells from three patients with leukemia in relapse increased in numbers in short-term suspension cultures. This increase was dependent on the presence of either feeder populations containing a high percentage of blast cells or conditioned medium derived from such populations. Aneuploid cells present in direct marrow preparations were also prevalent in the cultures after 10 days. The apparent specificity of the increase for leukemic populations may provide a new approach to the study of leukemic blast cells.

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