scholarly journals Peripheral blood blast cell progenitors in human preleukemia

Blood ◽  
1982 ◽  
Vol 59 (1) ◽  
pp. 106-109 ◽  
Author(s):  
JS Senn ◽  
HA Messner ◽  
PH Pinkerton ◽  
L Chang ◽  
B Nitsch ◽  
...  
Keyword(s):  
Acute Leukemia ◽  
Clinical Significance ◽  
Peripheral Blood ◽  
Tritiated Thymidine ◽  
Blast Cell ◽  
Short Term ◽  
Short Term Exposure

Abstract Progenitors of blast cell colonies have been identified in acute leukemia. The peripheral blood of 18 of 25 patients with preleukemic states yielded low numbers of blast cell colonies, and the colony- forming cells were in an active proliferative state when assessed using short-term exposure to tritiated thymidine. The clinical significance of blast cell colonies is uncertain, but we suggest that further analysis of this cultural abnormality may lead to a better understanding of mechanisms and management in preleukemia.

scholarly journals Peripheral blood blast cell progenitors in human preleukemia

Blood ◽  
1982 ◽  
Vol 59 (1) ◽  
pp. 106-109
Author(s):  
JS Senn ◽  
HA Messner ◽  
PH Pinkerton ◽  
L Chang ◽  
B Nitsch ◽  
...  
Keyword(s):  
Acute Leukemia ◽  
Clinical Significance ◽  
Peripheral Blood ◽  
Tritiated Thymidine ◽  
Blast Cell ◽  
Short Term ◽  
Short Term Exposure

Progenitors of blast cell colonies have been identified in acute leukemia. The peripheral blood of 18 of 25 patients with preleukemic states yielded low numbers of blast cell colonies, and the colony- forming cells were in an active proliferative state when assessed using short-term exposure to tritiated thymidine. The clinical significance of blast cell colonies is uncertain, but we suggest that further analysis of this cultural abnormality may lead to a better understanding of mechanisms and management in preleukemia.

Gene Expression Analysis in Human Peripheral Blood Cells after 900 MHz RF-EMF Short-Term Exposure

2018 ◽  
Vol 189 (5) ◽  
pp. 529-540 ◽  
Author(s):  
Andreas Lamkowski ◽  
Matthias Kreitlow ◽  
Jörg Radunz ◽  
Martin Willenbockel ◽  
Frank Sabath ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Gene Expression Analysis ◽  
Human Peripheral Blood ◽  
Peripheral Blood Cells ◽  
Short Term ◽  
Short Term Exposure

URINARY AMINOIMIDAZOLECARBOXAMIDE LEVELS IN CHILDREN WITH ACUTE LEUKEMIA

PEDIATRICS ◽  
1970 ◽  
Vol 45 (6) ◽  
pp. 983-995
Author(s):  
Gary Lulenski ◽  
Milton Donaldson ◽  
David Newcombe
Keyword(s):  
Acute Leukemia ◽  
Peripheral Blood ◽  
Drug Toxicity ◽  
Blast Cell ◽  
Normal Children ◽  
Cell Content ◽  
Platelet Counts ◽  
Leukemic Relapse

Urinary 5-amino-4-imidazolecarbox-amide (AIC) was studied in 10 leukemic children to determine whether it reliably indicates relapse of the disease. Mean AIC in normal children was 0.92 µg/mg creatinine/24 hours; in leukemic remission it was 1.44, and in relapse it was 5.01. AIC levels correlated significantly with marrow blast cell content. less so with peripheral blood blasts, and inversely with platelet counts. Data suggest AIC may differentiate between leukopenia of relapse and that of drug toxicity, and that it may predict leukemic relapse even before diagnostic morphologic marrow changes occur. AIC is unreliable in patients on methotrexate because the drug causes an elevation of the urinary AIC level.

scholarly journals The Remission and Relapse Status of Acute Leukemia as Studied by the In Vitro Uptake of Tritiated Thymidine by Peripheral Blood Leukocytes

Blood ◽  
1968 ◽  
Vol 32 (2) ◽  
pp. 292-304
Author(s):  
THEODORE S. ZIMMERMAN ◽  
HERMAN A. GODWIN ◽  
MARVIN ZELEN ◽  
SEYMOUR PERRY
Keyword(s):  
Acute Leukemia ◽  
Peripheral Blood ◽  
Normal Population ◽  
Tritiated Thymidine ◽  
Additional Parameter ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
Normal Controls ◽  
In Vitro Uptake

Abstract H3TdR uptake by peripheral blood leukocytes has been measured in patients with acute leukemia and in hematologically normal controls. Patients with acute leukemia in remission and relapse generally had elevated uptakes and formed populations distinct from the normal population and from each other, although overlap of values between each population was present. The measurement of H3TdR uptake may prove useful as an additional parameter for studying acute leukemia.

scholarly journals Pluripotent hemopoietic progenitors (CFU-GEMM) in polycythemia vera: analysis of erythropoietin requirement and proliferative activity

Blood ◽  
1981 ◽  
Vol 58 (6) ◽  
pp. 1224-1227 ◽  
Author(s):  
AA Fauser ◽  
HA Messner
Keyword(s):  
Polycythemia Vera ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Proliferative Activity ◽  
Growth Patterns ◽  
Short Term ◽  
Proliferative Rate ◽  
Normal Individuals ◽  
Short Term Exposure ◽  
Blood Specimens

Pluripotent hemopoietic progenitors (CFU-GEMM) give rise to multilineage hemopoietic colonies in culture. We have examined the erythropoietin requirements of CFU-GEMM-derived erythroid progeny in patients with polycythemia vera (PV) and studied their proliferative activity by short-term exposure to 3HTdR. Mixed colonies with erythroid components were observed in all bone marrow and peripheral blood samples from patients with PV that were cultured without addition of exogenous erythropoietin. This response is consistent with previously reported growth patterns for CFU-E and BFU-E. The frequency of mixed colonies increased regularly when erythropoietin was added to the cultures. Short-term exposure of peripheral blood specimens to 3HTdR prior to plating yielded a reduction of the plating efficiency by 20%- 70% when compared to cells that were not exposed to 3HTdR. The observation of cycling CFU-GEMM in PV contrasts with the usually quiescent behavior of CFU-GEMM in peripheral blood of normal individuals under steady-state conditions. These results support the view that the increased proliferative rate observed for CFU-GEMM may be responsible for the increased formation of blood cells in PV.

scholarly journals Brief Note: Tritiated Thymidine as a Cytocidal Agent in Human Leukemia

Blood ◽  
1966 ◽  
Vol 28 (6) ◽  
pp. 851-859 ◽  
Author(s):  
MICHAEL L. GREENBERG ◽  
ARJUN D. CHANANA ◽  
EUGENE P. CRONKITE ◽  
LEWIS M. SCHIFFER ◽  
PIERRE A. STRYCKMANS
Keyword(s):  
Body Weight ◽  
Acute Leukemia ◽  
Peripheral Blood ◽  
Tritiated Thymidine ◽  
Leukemic Cells ◽  
Radiation Doses ◽  
Human Leukemia ◽  
Cytotoxic Effects

Abstract 1. Two patients with acute leukemia had considerable decreases in leukemic cells in the peripheral blood as well as reduction in size of spleen and leukemic masses after 10 injections of 3H-TDR given over a 5-day period. Each injection was 0.25 µc./Gm. body weight. 2. The pertinent aspects of cytotoxic effects of 3H-TDR are reviewed. 3. The radiation doses delivered to the nucleus are estimated from autoradiographic data. 4. Evidence is presented for the observed effects being due to 3H-TDR.

scholarly journals Pluripotent hemopoietic progenitors (CFU-GEMM) in polycythemia vera: analysis of erythropoietin requirement and proliferative activity

Blood ◽  
1981 ◽  
Vol 58 (6) ◽  
pp. 1224-1227 ◽  
Author(s):  
AA Fauser ◽  
HA Messner
Keyword(s):  
Polycythemia Vera ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Proliferative Activity ◽  
Growth Patterns ◽  
Short Term ◽  
Proliferative Rate ◽  
Normal Individuals ◽  
Short Term Exposure ◽  
Blood Specimens

Abstract Pluripotent hemopoietic progenitors (CFU-GEMM) give rise to multilineage hemopoietic colonies in culture. We have examined the erythropoietin requirements of CFU-GEMM-derived erythroid progeny in patients with polycythemia vera (PV) and studied their proliferative activity by short-term exposure to 3HTdR. Mixed colonies with erythroid components were observed in all bone marrow and peripheral blood samples from patients with PV that were cultured without addition of exogenous erythropoietin. This response is consistent with previously reported growth patterns for CFU-E and BFU-E. The frequency of mixed colonies increased regularly when erythropoietin was added to the cultures. Short-term exposure of peripheral blood specimens to 3HTdR prior to plating yielded a reduction of the plating efficiency by 20%- 70% when compared to cells that were not exposed to 3HTdR. The observation of cycling CFU-GEMM in PV contrasts with the usually quiescent behavior of CFU-GEMM in peripheral blood of normal individuals under steady-state conditions. These results support the view that the increased proliferative rate observed for CFU-GEMM may be responsible for the increased formation of blood cells in PV.

scholarly journals Genotoxicity assessment of fipronil (frontline plus®) in Canis familiaris

2017 ◽  
Vol 37 (3) ◽  
pp. 257-260
Author(s):  
Liane Ziliotto ◽  
Stelio P.L. Luna ◽  
Daniel A. A.Filho ◽  
Ludimila O. Resende ◽  
Aline G. Aun ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Blood Cells ◽  
Canis Familiaris ◽  
Neck Region ◽  
Domestic Animals ◽  
Genotoxic Effect ◽  
Single Exposure ◽  
Short Term ◽  
Short Term Exposure ◽  
First Time

ABSTRACT: Fipronil is a pesticide widely used for controlling fleas and ticks in domestic animals, and its short-term exposure can lead to serious effects on animals. However, the possible genotoxic effect of this compound has not been investigated in target animals. Based on the hypothesis that fipronil can induce genotoxicity, this study evaluated the effect of fipronil on DNA damage in peripheral blood cells. For that purpose, ten dogs of both sexes were used in the study. The product (6.7mg/kg) was applied on the dorsal neck region of each animal. Peripheral blood samples were collected immediately prior to application of the product, and at 3, 8 and 24 hours after the application. Samples were processed for comet assay. No statistically significant differences were found among the four time points. The current study suggests for the first time that a single exposure to this pesticide does not induce systemic genotoxic effect in dogs.

scholarly journals Proliferative state of human pluripotent hemopoietic progenitors (CFU- GEMM) in normal individuals and under regenerative conditions after bone marrow transplantation

Blood ◽  
1979 ◽  
Vol 54 (5) ◽  
pp. 1197-1200 ◽  
Author(s):  
AA Fauser ◽  
HA Messner
Keyword(s):  
Bone Marrow ◽  
Tritiated Thymidine ◽  
Marrow Transplant ◽  
Suicide Method ◽  
Short Term ◽  
Normal Bone ◽  
Normal Individuals ◽  
Short Term Exposure ◽  
Bone Marrow Regeneration ◽  
Early Regeneration

Abstract The proliferative state of human pluripotent hemopoietic progenitors (CFU-GEMM) was assessed in normal bone marrow transplant donors and in recipients after engraftment using the tritiated thymidine suicide method. In contrast to BFU-E and CFU-C, CFU-GEMM were found to be quiescent. In samples obtained during early regeneration after transplantation, CFU-GEMM were significantly reduced after short-term exposure to 3H TdR when compared to controls. This observation suggests active participation of CFU-GEMM in bone marrow regeneration.

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