scholarly journals Lactate dehydrogenase isoenzymes in normal and malignant human lymphoid cells

Blood ◽  
1982 ◽  
Vol 60 (2) ◽  
pp. 491-494 ◽  
Author(s):  
J Blatt ◽  
RJ Spiegel ◽  
NM Papadopoulos ◽  
SA Lazarou ◽  
IT Magrath ◽  
...  
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Lactate Dehydrogenase ◽  
T Cell ◽  
Lymphoblastic Leukemia ◽  
Diagnostic Category ◽  
Lymphocytic Leukemia ◽  
Lymphoid Cells ◽  
Lymphoid Malignancies ◽  
Cell Acute Lymphoblastic Leukemia ◽  
Isoenzyme Patterns

Abstract Intracellular lactate dehydrogenase (LD) isoenzyme patterns were studied in the malignant cells of patients with a variety of lymphoid malignancies. These were compared with intracellular LD isoenzyme patterns of normal lymphoid cells and were also correlated with immunologic cell surface marker characteristics. Results showed that, in general, the malignant B cells of Burkitt's lymphoma and the lymphoblasts of T-cell acute lymphoblastic leukemia had isoenzyme patterns similar to those of normal B and T cells, respectively. The isoenzyme patterns of malignant lymphoid cells from patients with non- T, and non-B acute lymphoblastic leukemia, cutaneous T-cell lymphoma, and chronic lymphocytic leukemia were more heterogeneous. These data, although based on small numbers of patients, are consistent with the hypothesis that LD isoenzymes may reflect differences in the maturational status of cells within a single diagnostic category.

scholarly journals Lactate dehydrogenase isoenzymes in normal and malignant human lymphoid cells

Blood ◽  
1982 ◽  
Vol 60 (2) ◽  
pp. 491-494
Author(s):  
J Blatt ◽  
RJ Spiegel ◽  
NM Papadopoulos ◽  
SA Lazarou ◽  
IT Magrath ◽  
...  
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Lactate Dehydrogenase ◽  
T Cell ◽  
Lymphoblastic Leukemia ◽  
Diagnostic Category ◽  
Lymphocytic Leukemia ◽  
Lymphoid Cells ◽  
Lymphoid Malignancies ◽  
Cell Acute Lymphoblastic Leukemia ◽  
Isoenzyme Patterns

Intracellular lactate dehydrogenase (LD) isoenzyme patterns were studied in the malignant cells of patients with a variety of lymphoid malignancies. These were compared with intracellular LD isoenzyme patterns of normal lymphoid cells and were also correlated with immunologic cell surface marker characteristics. Results showed that, in general, the malignant B cells of Burkitt's lymphoma and the lymphoblasts of T-cell acute lymphoblastic leukemia had isoenzyme patterns similar to those of normal B and T cells, respectively. The isoenzyme patterns of malignant lymphoid cells from patients with non- T, and non-B acute lymphoblastic leukemia, cutaneous T-cell lymphoma, and chronic lymphocytic leukemia were more heterogeneous. These data, although based on small numbers of patients, are consistent with the hypothesis that LD isoenzymes may reflect differences in the maturational status of cells within a single diagnostic category.

scholarly journals MiR-7 Functions as a Tumor Suppressor by Targeting the Oncogenes TAL1 in T-Cell Acute Lymphoblastic Leukemia

2020 ◽  
Vol 19 ◽  
pp. 153303382093413
Author(s):  
Hongbo Sun ◽  
Zhifu Zhang ◽  
Wei Luo ◽  
Junmin Liu ◽  
Ye Lou ◽  
...  
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
T Cell ◽  
Tumor Suppressor ◽  
Lymphoblastic Leukemia ◽  
Critical Role ◽  
Ectopic Expression ◽  
Lymphocytic Leukemia ◽  
Leukemia Cells ◽  
Cell Acute Lymphoblastic Leukemia ◽  
And Migration

Background: T-cell acute lymphoblastic leukemia is a hematologic malignancy characterized by T-cell proliferation, and in many cases, the ectopic expression of the oncogenic transcription factor T-cell acute lymphocytic leukemia protein 1 (TAL1). MicroRNA-7 has been shown to play a critical role in proliferation, migration, and treatment sensitivity in a diverse array of cancers. In this study, we sought to establish a novel link between microRNA-7 and T-cell acute lymphoblastic leukemia oncogenesis. Material and Method: To do so, we characterized gene expression of microRNA-7 as well as TAL1 in both T-cell acute lymphoblastic leukemia patient-derived tissue and cell lines, as well as performing functional luciferase assays to assess microRNA-7 binding to the TAL1 3′-untranslated region. We also performed growth, apoptosis, and migration experiments using 3-(4,5-dimethylthiazol-2-Yl)-2,5-diphenyltetrazolium bromide, Annexin V, and transwell assays in the context of microRNA-7 overexpression. Results: We found that microRNA-7 expression is attenuated and inversely correlated with TAL1 expression in TAL1 + T-cell acute lymphoblastic leukemia cells. Additionally, microRNA-7 directly targets and suppresses TAL1 levels. Finally, microRNA-7 overexpression reduces growth, motility, and migration while inducing apoptosis in T-cell acute lymphoblastic leukemia cells, phenotypes that can be rescued by concomitant overexpression of TAL1. Conclusions: These results indicate that microRNA-7 functions as a potent tumor suppressor by inhibiting the oncogene TAL1 and suggest microRNA-7 could function as a prognostic biomarker and possible therapeutic in the clinical management of T-cell acute lymphoblastic leukemia.

scholarly journals Targeting lactate dehydrogenase A ( LDHA ) exerts antileukemic effects on T‐cell acute lymphoblastic leukemia

2020 ◽  
Vol 40 (10) ◽  
pp. 501-517
Author(s):  
Haizhi Yu ◽  
Yafei Yin ◽  
Yifang Yi ◽  
Zhao Cheng ◽  
Wenyong Kuang ◽  
...  
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Lactate Dehydrogenase ◽  
T Cell ◽  
Lymphoblastic Leukemia ◽  
Cell Acute Lymphoblastic Leukemia

PTPN2 negatively regulates oncogenic JAK1 in T-cell acute lymphoblastic leukemia

Blood ◽  
2011 ◽  
Vol 117 (26) ◽  
pp. 7090-7098 ◽  
Author(s):  
Maria Kleppe ◽  
Jean Soulier ◽  
Vahid Asnafi ◽  
Nicole Mentens ◽  
Tekla Hornakova ◽  
...  
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
T Cell ◽  
Chromosomal Abnormalities ◽  
Lymphoblastic Leukemia ◽  
Tyrosine Phosphatase ◽  
Strong Association ◽  
Gene Mutations ◽  
Lymphoid Cells ◽  
Negative Regulator ◽  
Cell Acute Lymphoblastic Leukemia

We have recently reported inactivation of the tyrosine phosphatase PTPN2 (also known as TC-PTP) through deletion of the entire gene locus in ∼ 6% of T-cell acute lymphoblastic leukemia (T-ALL) cases. T-ALL is an aggressive disease of the thymocytes characterized by the stepwise accumulation of chromosomal abnormalities and gene mutations. In the present study, we confirmed the strong association of the PTPN2 deletion with TLX1 and NUP214-ABL1 expression. In addition, we found cooperation between PTPN2 deletion and activating JAK1 gene mutations. Activating mutations in JAK1 kinase occur in ∼ 10% of human T-ALL cases, and aberrant kinase activity has been shown to confer proliferation and survival advantages. Our results reveal that some JAK1 mutation–positive T-ALLs harbor deletions of the tyrosine phosphatase PTPN2, a known negative regulator of the JAK/STAT pathway. We provide evidence that down-regulation of Ptpn2 sensitizes lymphoid cells to JAK1-mediated transformation and reduces their sensitivity to JAK inhibition.

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