Lack of Rhesus antigen expression by human committed erythroid progenitors

The D antigen of the Rhesus blood group, an erythroid-specific cell surface marker, is expressed by all morphologically recognizable human nucleated red blood cell precursors including, in low density, the pronormoblast. The object of the present study was to determine the expression of the D antigen by committed erythroid progenitors. Under conditions that produced complete inhibition of BFU-E and CFU-E by known cytotoxic antisera, no significant inhibition was produced by anti-D. Use of anti-human IgG (rabbit) to increase sensitivity and trypsinization to reveal cryptic Rh determinants were both without inhibitory effect. Erythroid bursts and colonies grew normally in methylcellulose that contained anti-D. The addition of anti-D to day 7 BFU-E did not inhibit their proliferation to mature bursts at day 14. These results suggest that the D antigen is not expressed by human committed erythroid progenitor cells. The D antigen is therefore an erythroid-specific differentiation marker, rather than an erythroid- lineage-specific antigen. The development of expression of the D antigen during erythropoiesis parallels that of band 3 protein, to which anti-D has been reported to bind. Lack of Rh expression by committed erythroid progenitors is consistent with the rarity of red cell aplasia in Rhesus hemolytic disease of the newborn and in idiopathic and drug-induced autoimmune hemolytic anemia in which the autoantibodies have apparent Rh specificity. These results imply that Rh compatibility is not a contraindication to human bone marrow transplantation.

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Lack of Rhesus antigen expression by human committed erythroid progenitors

Blood ◽

10.1182/blood.v61.3.525.525 ◽

1983 ◽

Vol 61 (3) ◽

pp. 525-529 ◽

Cited By ~ 6

Author(s):

A Rearden ◽

P Chiu

Keyword(s):

Specific Antigen ◽

Antigen Expression ◽

Inhibitory Effect ◽

Significant Inhibition ◽

Specific Cell ◽

Erythroid Progenitors ◽

Drug Induced ◽

Erythroid Progenitor ◽

Differentiation Marker ◽

D Antigen

Abstract The D antigen of the Rhesus blood group, an erythroid-specific cell surface marker, is expressed by all morphologically recognizable human nucleated red blood cell precursors including, in low density, the pronormoblast. The object of the present study was to determine the expression of the D antigen by committed erythroid progenitors. Under conditions that produced complete inhibition of BFU-E and CFU-E by known cytotoxic antisera, no significant inhibition was produced by anti-D. Use of anti-human IgG (rabbit) to increase sensitivity and trypsinization to reveal cryptic Rh determinants were both without inhibitory effect. Erythroid bursts and colonies grew normally in methylcellulose that contained anti-D. The addition of anti-D to day 7 BFU-E did not inhibit their proliferation to mature bursts at day 14. These results suggest that the D antigen is not expressed by human committed erythroid progenitor cells. The D antigen is therefore an erythroid-specific differentiation marker, rather than an erythroid- lineage-specific antigen. The development of expression of the D antigen during erythropoiesis parallels that of band 3 protein, to which anti-D has been reported to bind. Lack of Rh expression by committed erythroid progenitors is consistent with the rarity of red cell aplasia in Rhesus hemolytic disease of the newborn and in idiopathic and drug-induced autoimmune hemolytic anemia in which the autoantibodies have apparent Rh specificity. These results imply that Rh compatibility is not a contraindication to human bone marrow transplantation.

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Chimeric Maternal Cells with Tissue-Specific Antigen Expression and Morphology Are Common in Infant Tissues

Pediatric and Developmental Pathology ◽

10.2350/08-07-0499.1 ◽

2009 ◽

Vol 12 (5) ◽

pp. 337-346 ◽

Cited By ~ 26

Author(s):

Anne M. Stevens ◽

Heidi M. Hermes ◽

Meghan M. Kiefer ◽

Joe C. Rutledge ◽

J. Lee Nelson

Keyword(s):

Islet Cells ◽

Tissue Injury ◽

Specific Antigen ◽

Antigen Expression ◽

Cell Types ◽

Target Cells ◽

Specific Cell ◽

Tissue Specific ◽

Renal Tubular Cells ◽

Parenchymal Cells

Maternal microchimerism (MMc) has been purported to play a role in the pathogenesis of autoimmunity, but how a small number of foreign cells could contribute to chronic, systemic inflammation has not been explained. Reports of peripheral blood cells differentiating into tissue-specific cell types may shed light on the problem in that chimeric maternal cells could act as target cells within tissues. We investigated MMc in tissues from 7 male infants. Female cells, presumed maternal, were characterized by simultaneous immunohistochemistry and fluorescence in situ hybridization for X- and Y-chromosomes. Maternal cells constituted 0.017% to 1.9% of parenchymal cells and were found in all infants in liver, pancreas, lung, kidney, bladder, skin, and spleen. Maternal cells were differentiated: maternal hepatocytes in liver, renal tubular cells in kidney, and β-islet cells in pancreas. Maternal cells were not found in areas of tissue injury or inflammatory infiltrate. Maternal hematopoietic cells were found only in hearts from patients with neonatal lupus. Thus, differentiated maternal cells are present in multiple tissue types and occur independently of inflammation or tissue injury. Loss of tolerance to maternal parenchymal cells could lead to organ-specific “auto” inflammatory disease and elimination of maternal cells in areas of inflammation.

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Anti-Human Immunodeficiency Virus (HIV) Activity of the Novel Antiviral Antibiotic Quartromicins which Enhance Inhibitory Effect of 3′-azido-2′,3′-dideoxythymidine (AZT) in vitro

Antiviral Chemistry and Chemotherapy ◽

10.1177/095632029200300604 ◽

1992 ◽

Vol 3 (6) ◽

pp. 345-349 ◽

Cited By ~ 10

Author(s):

A. Tanabe-Tochikura ◽

H. Nakashima ◽

T. Murakami ◽

O. Tenmyo ◽

T. Oki ◽

...

Keyword(s):

Human Immunodeficiency Virus ◽

Specific Antigen ◽

Antigen Expression ◽

Antiviral Effect ◽

Inhibitory Effect ◽

Reverse Transcriptase Activity ◽

The Novel ◽

Avian Myeloblastosis Virus ◽

Immunodeficiency Virus

Novel antiviral antibiotic quartromicins A1 and D1, isolated from Amycolatopsis orientalis, significantly inhibited human immunodeficiency virus (HIV)-induced cytopathic effect and virus specific antigen expression at concentrations of 25–100 μg ml−1 In MT-4 cells infected with HTLV-IIIB. The reverse transcriptase activity of disrupted HTLV-IIIB particles, recombinant HIV-1 enzyme, and purified avian myeloblastosis virus (AMV) enzyme were also significantly inhibited by quartromicins A1 and D1. The combined antiviral effect of quartromicin A1 and AZT on the replication of HIV in MT-4 cells was also examined. Quartromicin A1 synergistically enhanced the inhibitory effect of AZT as revealed by HIV-specific antigen expression.

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A comparative study of the antibacterial activity of Quercus robur (Ethanolic extract) and Zinc oxide nanoparticles on Salmonella (In vitro)

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v9ispl1.1397 ◽

2018 ◽

Vol 9 (SPL1) ◽

Author(s):

Hams H. H. Alfattli ◽

Ghufran Zuhair Jiber ◽

Ghaidaa Gatea Abbass

Keyword(s):

Zinc Oxide ◽

Antibacterial Activity ◽

Zinc Oxide Nanoparticles ◽

Quercus Robur ◽

Ethanolic Extract ◽

Inhibitory Effect ◽

Significant Inhibition ◽

Oxide Nanoparticles ◽

Inhibition Zones

This study which designed to evaluate the inhibitory effect of Ethanolic extract of (Quercusrobur) and Zinc oxide nanoparticles on the growth of one genus of enterobacteriacae (Salmonella). In vitro. For this purpose graduate concentrates for plant extract (50, 100, 200, 400 )mg/ml which prepared and compared with Zinc oxide nanoparticles of different concentration (2, 1, 0.5, 0.25) μg/ml,and examined. The result showed that the studied medicinal plant has antibacterial activity against this bacteria which used. The result showed that the plant has good activity in decrease the growth of this bacteria. The results of the study also showed that the nano-ZnO has very effective antibacterial action against the studied bacteria which was Salmonella,nanoparticles concentrations lead to increasing in the inhibition zones of tested bacterial growth. We also study the effect of three antibiotics Lomefloxacin (LOM), Ciprofloxacin (SIP) and Rifampin (RA) and the result showed,in a comparison within the tested bacteria,Salmonella had a significant inhibition increase in Lomefloxacin ; the ciprofloxacin showed effect on tested bacteria. However,Rifampin does not show any effect on tested bacteria.

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Missense RHD single nucleotide variants induce weakened D antigen expression by altering splicing and/or protein expression

Transfusion ◽

10.1111/trf.16538 ◽

2021 ◽

Author(s):

Loann Raud ◽

Marlène Le Tertre ◽

Léonie Vigneron ◽

Chandran Ka ◽

Gaëlle Richard ◽

...

Keyword(s):

Protein Expression ◽

Antigen Expression ◽

Single Nucleotide Variants ◽

Single Nucleotide ◽

D Antigen

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Fetal calf serum contains activities that induce fetal hemoglobin in adult erythroid cell cultures

Blood ◽

10.1182/blood.v75.9.1862.1862 ◽

1990 ◽

Vol 75 (9) ◽

pp. 1862-1869 ◽

Cited By ~ 13

Author(s):

P Constantoulakis ◽

B Nakamoto ◽

T Papayannopoulou ◽

G Stamatoyannopoulos

Keyword(s):

Cell Cultures ◽

Fetal Calf Serum ◽

Calf Serum ◽

Fetal Hemoglobin ◽

Erythroid Cell ◽

Erythroid Progenitors ◽

Globin Mrna ◽

Erythroid Progenitor ◽

Fetal Sheep ◽

Gamma Globin

Abstract Cultures of peripheral blood or bone marrow erythroid progenitors display stimulated production of fetal hemoglobin. We investigated whether this stimulation is due to factors contained in the sera of the culture medium. Comparisons of gamma/gamma + beta biosynthetic ratios in erythroid colonies grown in fetal calf serum (FCS) or in charcoal treated FCS (C-FCS) showed that FCS-grown cells had significantly higher gamma/gamma + beta ratios. This increase in globin chain biosynthesis was reflected by an increase in relative amounts of steady- state gamma-globin mRNA. In contrast to its effect on adult cells, FCS failed to influence gamma-chain synthesis in fetal burst forming units- erythroid (BFU-E) colonies. There was a high correlation of gamma- globin expression in paired cultures done with C-FCS or fetal sheep serum. Dose-response experiments showed that the induction of gamma- globin expression is dependent on the concentration of FCS. These results indicate that FCS contains an activity that induces gamma- globin expression in adult erythroid progenitor cell cultures.

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