Microrheologic investigation of erythrocyte deformability in diabetes mellitus

Abstract This study was undertaken to determine whether diabetes alters the viscoelastic properties of erythrocytes. The oldest and youngest 10% fractions of circulating red cells were separated by centrifugation of freshly drawn blood obtained from ten diabetics with disease of one to 20 years' duration and from an equal number of age- and sex-matched control subjects. Cells from each fraction were suspended in phosphate- buffered saline, and their rheologic behavior was examined in a rheoscope. The elongation of cells, the percentage of cells that tank- treaded in response to shear stress, tank-treading frequency, and the rate of recovery of cell shape upon cessation of shear stress were determined in the oldest and youngest 10% of cells for diabetics as well as for controls. All four parameters were virtually identical for diabetics and controls. Additional aliquots of cells were taken for assessment of nonenzymatic glucosylation of hemoglobin and cell membrane protein. The absence of any measurable difference in rheologic behavior of cells from diabetic and control subjects, despite substantial differences in nonenzymatic glucosylation of hemoglobin and cell membrane proteins, suggests that the magnitude of glucosylation observed in these cellular constituents does not alter the viscoelastic properties of the cells. The implication of these observations is that microvascular complications of diabetes are not attributable to altered deformability of red cells.

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Microrheologic investigation of erythrocyte deformability in diabetes mellitus

Blood ◽

10.1182/blood.v65.2.283.bloodjournal652283 ◽

1985 ◽

Vol 65 (2) ◽

pp. 283-288

Author(s):

JR Williamson ◽

RA Gardner ◽

CW Boylan ◽

GL Carroll ◽

K Chang ◽

...

Keyword(s):

Shear Stress ◽

Cell Membrane ◽

Viscoelastic Properties ◽

Microvascular Complications ◽

Red Cells ◽

Control Subjects ◽

Cell Membrane Proteins ◽

Rheologic Behavior ◽

Cell Membrane Protein ◽

And Control

This study was undertaken to determine whether diabetes alters the viscoelastic properties of erythrocytes. The oldest and youngest 10% fractions of circulating red cells were separated by centrifugation of freshly drawn blood obtained from ten diabetics with disease of one to 20 years' duration and from an equal number of age- and sex-matched control subjects. Cells from each fraction were suspended in phosphate- buffered saline, and their rheologic behavior was examined in a rheoscope. The elongation of cells, the percentage of cells that tank- treaded in response to shear stress, tank-treading frequency, and the rate of recovery of cell shape upon cessation of shear stress were determined in the oldest and youngest 10% of cells for diabetics as well as for controls. All four parameters were virtually identical for diabetics and controls. Additional aliquots of cells were taken for assessment of nonenzymatic glucosylation of hemoglobin and cell membrane protein. The absence of any measurable difference in rheologic behavior of cells from diabetic and control subjects, despite substantial differences in nonenzymatic glucosylation of hemoglobin and cell membrane proteins, suggests that the magnitude of glucosylation observed in these cellular constituents does not alter the viscoelastic properties of the cells. The implication of these observations is that microvascular complications of diabetes are not attributable to altered deformability of red cells.

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Shear Stress-Enhanced Internalization of Cell Membrane Proteins Indicated by a Hairpin-Type DNA Probe

Analytical Chemistry ◽

10.1021/acs.analchem.8b00755 ◽

2018 ◽

Vol 90 (9) ◽

pp. 5540-5545 ◽

Cited By ~ 15

Author(s):

Ziyi He ◽

Wanling Zhang ◽

Sifeng Mao ◽

Nan Li ◽

Haifang Li ◽

...

Keyword(s):

Shear Stress ◽

Membrane Proteins ◽

Cell Membrane ◽

Dna Probe ◽

Cell Membrane Proteins

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The viscoelastic properties of monolayers of red cell membrane proteins

Journal of Colloid and Interface Science ◽

10.1016/0021-9797(79)90080-8 ◽

1979 ◽

Vol 69 (1) ◽

pp. 67-73 ◽

Cited By ~ 14

Author(s):

M Blank ◽

R.G King ◽

L Soo ◽

R.E Abbott ◽

S Chien

Keyword(s):

Membrane Proteins ◽

Cell Membrane ◽

Viscoelastic Properties ◽

Red Cell ◽

Red Cell Membrane ◽

Cell Membrane Proteins

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Viscoelastic properties of the human red blood cell membrane. II. Area and volume of individual red cells entering a micropipette

Biophysical Journal ◽

10.1016/s0006-3495(77)85634-8 ◽

1977 ◽

Vol 17 (2) ◽

pp. 169-178 ◽

Cited By ~ 19

Author(s):

A.W. Jay ◽

P.B. Canham

Keyword(s):

Cell Membrane ◽

Blood Cell ◽

Red Blood Cell ◽

Viscoelastic Properties ◽

Red Cells ◽

Red Blood Cell Membrane ◽

Area And Volume

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Candidate screening of host cell membrane proteins involved in SARS-CoV-2 entry

10.1101/2020.09.09.289488 ◽

2020 ◽

Author(s):

Norihiro Kotani ◽

Takanari Nakano

Keyword(s):

Membrane Proteins ◽

Cell Membrane ◽

Host Cell ◽

Viral Entry ◽

Antiviral Agents ◽

Host Cells ◽

Host Cell Membrane ◽

Cell Membrane Proteins ◽

Cell Membrane Protein ◽

Spike Proteins

ABSTRACTCOVID-19 represents a real threat to the global population, and understanding the biological features of the causative virus (SARS-CoV-2) is imperative to aid in mitigating this threat. Analyses of proteins such as primary receptors and co-receptors (co-factors) that are involved in SARS-CoV-2 entry into host cells will provide important clues to help control the virus. Here, we identified host cell membrane protein candidates that were present in proximity to the attachment sites of SARS-CoV-2 spike proteins through the use of proximity labeling and proteomics analysis. The identified proteins represent candidate key factors that may be required for viral entry. Our results indicated that a number of membrane proteins, including DPP4, Cadherin-17, and CD133, were identified to co-localize with cell membrane-bound SARS-CoV-2 spike proteins in Caco-2 cells that were used to expand the SARS-CoV-2 virion. We anticipate that the information regarding these protein candidates will be utilized for the future development of vaccines and antiviral agents against SARS-CoV-2.

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The Effects of a Neutral Stimulus (Buzzer) on Motor Responses and Disfluencies in Normal Speakers

Journal of Speech and Hearing Research ◽

10.1044/jshr.1201.179 ◽

1969 ◽

Vol 12 (1) ◽

pp. 179-184 ◽

Cited By ~ 7

Author(s):

Richard R. Martin ◽

Gerald M. Siegel

Keyword(s):

College Students ◽

Neutral Stimulus ◽

Motor Responses ◽

Control Subjects ◽

And Control ◽

Speaking Task

Seventy-two college students were divided into three groups: Button Push-Speech (BP-S), Speech-Button Push (S-BP), and Control. BP-S subjects pushed one of two buttons on signal for 8 min. During the last 4 min, depression of the criterion button caused a buzzer to sound. After the button-push task, subjects spoke spontaneously for 30 min. During the last 20 min, the buzzer was presented contingent upon each disfluency. S-BP subjects were run under the same procedures, but the order of button-push and speech tasks was reversed. Control subjects followed the same procedures as S-BP subjects, but no buzzer signal was presented at any time. Both S-BP and BP-S subjects emitted significantly fewer disfluencies during the last 20 min (Conditioning) than during the first 10 min (Baserate) of the speaking task. The frequency of disfluencies for Control subjects did not change significantly from Baserate to Conditioning. In none of the three groups did the frequency of pushes on the criterion button change significantly from minute to minute throughout the 8-min button-push session.

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Binding of a novel cell membrane protein to terminal complement complexes

Immunology Letters ◽

10.1016/s0165-2478(97)87277-x ◽

1997 ◽

Vol 56 (1-3) ◽

pp. 110-111

Author(s):

J Tissari

Keyword(s):

Cell Membrane ◽

Membrane Protein ◽

Cell Membrane Protein ◽

Terminal Complement Complexes ◽

Terminal Complement

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Tests for Platelet Changes, Acute Phase Reactants and Serum Lipids in Diabetes mellitus and Peripheral Vascular Disease

Thrombosis and Haemostasis ◽

10.1055/s-0038-1657283 ◽

1982 ◽

Vol 48 (03) ◽

pp. 289-293 ◽

Cited By ~ 17

Author(s):

B A van Oost ◽

B F E Veldhuyzen ◽

H C van Houwelingen ◽

A P M Timmermans ◽

J J Sixma

Keyword(s):

Diabetes Mellitus ◽

Vascular Disease ◽

Peripheral Vascular Disease ◽

Acute Phase ◽

Serum Lipids ◽

Diabetic Patients ◽

Peripheral Vascular ◽

Acute Phase Reactants ◽

Control Subjects ◽

And Control

SummaryPlatelets tests, acute phase reactants and serum lipids were measured in patients with diabetes mellitus and patients with peripheral vascular disease. Patients frequently had abnormal platelet tests and significantly increased acute phase reactants and serum lipids, compared to young healthy control subjects. These differences were compared with multidiscriminant analysis. Patients could be separated in part from the control subjects with variables derived from the measurement of acute phase proteins and serum lipids. Platelet test results improved the separation between diabetics and control subjects, but not between patients with peripheral vascular disease and control subjects. Diabetic patients with severe retinopathy frequently had evidence of platelet activation. They also had increased acute phase reactants and serum lipids compared to diabetics with absent or nonproliferative retinopathy. In patients with peripheral vascular disease, only the fibrinogen concentration was related to the degree of vessel damage by arteriography.

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