scholarly journals Eosinophils do respond to fMLP

Blood ◽  
1987 ◽  
Vol 70 (2) ◽  
pp. 379-383
Author(s):  
M Yazdanbakhsh ◽  
CM Eckmann ◽  
L Koenderman ◽  
AJ Verhoeven ◽  
D Roos
Keyword(s):  
Oxygen Consumption ◽  
Maximal Response ◽  
Free Calcium ◽  
O2 Consumption ◽  
Blood Samples ◽  
Nitroblue Tetrazolium Reduction ◽  
Percoll Gradients ◽  
Normal Human ◽  
Tetrazolium Reduction ◽  
Normal Human Blood

Eosinophils were isolated from normal human blood by separation over Percoll gradients, which resulted in eosinophil suspensions of a purity higher than 95% and recoveries of about 65%. Normal human eosinophils were found to respond to formyl-methionyl-leucyl-phenylalanine (fMLP) at concentrations greater than 10(-7) mol/L with an increase in the concentration of intracellular free calcium, oxygen consumption, nitroblue tetrazolium reduction, and chemiluminescence. The maximal response of eosinophils to fMLP was lower than that of neutrophils isolated from the same blood samples and required at least ten times as much fMLP as was needed for neutrophils. Low fMLP concentrations (approximately 10(-8) mol/L), which in themselves did not stimulate O2 consumption by either eosinophils or neutrophils, primed these cells to respond to a suboptimal concentration of another stimulus. Purification of eosinophils after treatment of whole blood with fMLP showed that these eosinophils had lost their ability to respond to fMLP. We conclude that normal eosinophils do respond to fMLP and that therefore fMLP should not be used to isolate eosinophils.

Eosinophils do respond to fMLP

Blood ◽  
1987 ◽  
Vol 70 (2) ◽  
pp. 379-383 ◽  
Author(s):  
M Yazdanbakhsh ◽  
CM Eckmann ◽  
L Koenderman ◽  
AJ Verhoeven ◽  
D Roos
Keyword(s):  
Oxygen Consumption ◽  
Maximal Response ◽  
Free Calcium ◽  
O2 Consumption ◽  
Blood Samples ◽  
Nitroblue Tetrazolium Reduction ◽  
Percoll Gradients ◽  
Normal Human ◽  
Tetrazolium Reduction ◽  
Normal Human Blood

Abstract Eosinophils were isolated from normal human blood by separation over Percoll gradients, which resulted in eosinophil suspensions of a purity higher than 95% and recoveries of about 65%. Normal human eosinophils were found to respond to formyl-methionyl-leucyl-phenylalanine (fMLP) at concentrations greater than 10(-7) mol/L with an increase in the concentration of intracellular free calcium, oxygen consumption, nitroblue tetrazolium reduction, and chemiluminescence. The maximal response of eosinophils to fMLP was lower than that of neutrophils isolated from the same blood samples and required at least ten times as much fMLP as was needed for neutrophils. Low fMLP concentrations (approximately 10(-8) mol/L), which in themselves did not stimulate O2 consumption by either eosinophils or neutrophils, primed these cells to respond to a suboptimal concentration of another stimulus. Purification of eosinophils after treatment of whole blood with fMLP showed that these eosinophils had lost their ability to respond to fMLP. We conclude that normal eosinophils do respond to fMLP and that therefore fMLP should not be used to isolate eosinophils.

Synthesis and solvatochromic studies of 2- amino-3-phenolazo1-(4-sulfophenyl)-3-methyi-5-pyrozolone and use it for the determination of trace amount of Nickel (II) in blood samples

2016 ◽  
Vol 5 (10) ◽  
pp. 4920
Author(s):  
Amar M. Ali ◽  
Hussain. J. Mohammed*
Keyword(s):  
Stability Constant ◽  
Trace Amount ◽  
Benzenesulfonic Acid ◽  
Determination Method ◽  
Blood Samples ◽  
Determination Of Nickel ◽  
Normal Human ◽  
The Stability ◽  
Normal Human Blood

A new, simple, sensitive and rapid spectrophotometric method is proposed for the determination of trace amount of Nickel (II). The method is based on the formation of a 1:2 complex with 4-(4-((2-hydroxy-6-nitrophenyl) diazenyl) -3-methyl-5-oxo-2, 5-dihydro-1H-pyrazol-1-yl) benzenesulfonic acid (2-ANASP) as a new reagent is developed. The complex has a maximum absorption at 516 nm and εmax of 1. 84 X 105 L. mol-1. cm-1. A linear correlation (0. 25 – 4. 0μg. ml-1) was found between absorbance at λmax and concentration. The accuracy and reproducibility of the determination method for various known amounts of Nickel (II) were tested. The results obtained are both precise (RSD was 1. 2 %) and accurate (relative error was 0. 787 %). The effect of diverse ions on the determination of Nickel (II) to investigate the selectivity of the method were also studied. The stability constant of the product was 0. 399 X 106 L. mol-1. The proposed method was successfully applied to the analysis of diabetes blood and normal human blood. 

scholarly journals IMMUNITY REACTIONS OF HUMAN SUBJECTS TO STRAINS OF PNEUMOCOCCI OTHER THAN TYPES I, II AND III

1933 ◽  
Vol 57 (1) ◽  
pp. 95-109 ◽  
Author(s):  
Maxwell Finland ◽  
W. D. Sutliff
Keyword(s):  
Human Serum ◽  
Human Blood ◽  
Human Subjects ◽  
Protective Action ◽  
Group Iv ◽  
Bactericidal Action ◽  
Blood Samples ◽  
Viii And Ix ◽  
Normal Human ◽  
Normal Human Blood

1. A group of 72 human subjects were studied with respect to the immune reactions of their blood and sera to Types I, II and III pneumococci and to 4 other types (V, VI, VIII and IX) previously included in Group IV. 2. The same general relationships were observed for all of these types as were previously demonstrated for Types I, II and III. Each type was specific in relation to the bactericidal action of normal human blood and the protective action of normal human serum. 3. The frequency with which pneumococcidal action for any pair of types was present for both or absent for both in the same blood samples was slightly greater than that calculated from the frequencies with which each of the types was killed separately. 4. No closer correlation could be demonstrated between the reaction of the blood of these subjects to Types II and V or between Types III and VIII pneumococci, types related in their reaction with artificially prepared immune sera, than was observed between unrelated strains.

scholarly journals The biochemical basis of nitroblue tetrazolium reduction in normal human and chronic granulomatous disease polymorphonuclear leukocytes

Blood ◽  
1976 ◽  
Vol 48 (2) ◽  
pp. 309-313 ◽  
Author(s):  
RL Baehner ◽  
LA Boxer ◽  
J Davis
Keyword(s):  
Chronic Granulomatous Disease ◽  
Polymorphonuclear Leukocytes ◽  
Nitroblue Tetrazolium ◽  
Granulomatous Disease ◽  
Biochemical Basis ◽  
Nitroblue Tetrazolium Reduction ◽  
Normal Human ◽  
Nbt Reduction ◽  
Human Polymorphonuclear Leukocytes ◽  
Tetrazolium Reduction

Normal human polymorphonuclear leukocytes (PMN) placed in anaerobic chambers reaching pO2's of less than 5 mm Hg fail to generate O2-, iodinate ingested particles, and stimulate glucose-1–14C oxidation through the hexose monophosphate shunt. The observation that anaerobic cells are incapable of generating O2- or reducing nitroblue tetrazolium (NBT) to formazan supports the idea that NBT reduction in phagocytizing PMN is due exclusively to oxygen-dependent O2- generating oxidase which is deficient in chronic granulomatous disease leukocytes, despite their hyperphagocytic capacity.

MINOR PROTEINS IN HUMAN ERYTHROCYTES

1961 ◽  
Vol 39 (10) ◽  
pp. 1489-1491 ◽  
Author(s):  
N. Chandrasekhar
Keyword(s):  
Human Serum ◽  
Human Blood ◽  
Agar Plate ◽  
Haemoglobin Concentration ◽  
Blood Samples ◽  
Low Concentrations ◽  
Electrophoresis Method ◽  
Normal Human ◽  
Human Blood Samples ◽  
Normal Human Blood

A study of 200 normal human blood samples, by the agar electrophoresis method, revealed that human haemolyzates contain two minor proteins in addition to the normal adult haemoglobin. The two minor proteins appearing in very low concentrations are non-haemoglobin in character, give a negative reaction to benzidine test, but a positive reaction to amido-schwarz. These two proteins have an electrophoretic mobility similar to that of γ-globulin of human serum, but appear as sharp distinct bands on the agar plate. The concentration of these non-haemoglobin components bear no correlation to the blood groups of the samples or to the haemoglobin concentration itself, but the components have some electrophoretic characteristics similar to some of the abnormal haemoglobins.

scholarly journals Inhibiting Effects of Human Plasma and Serum on Neutrophil Random Migration and Chemotaxis

Blood ◽  
1974 ◽  
Vol 44 (6) ◽  
pp. 843-848 ◽  
Author(s):  
H. U. Keller ◽  
M. W. Hess ◽  
H. Cottier
Keyword(s):  
Neutrophil Granulocytes ◽  
Neutrophil Chemotaxis ◽  
Chemotactic Migration ◽  
Experimental Conditions ◽  
Random Migration ◽  
The Past ◽  
Nitroblue Tetrazolium Reduction ◽  
Normal Human ◽  
Tetrazolium Reduction ◽  
Increased Susceptibility

Abstract Various authors have associated increased susceptibility to infectious diseases in certain patients to inhibitors of neutrophil chemotaxis demonstrable in serum or diluted plasma of these patients. The present experiments showed, however, that serum and diluted plasma but not undiluted plasma from normal human donors consistently inhibited chemotactic migration of autologous human neutrophil granulocytes. Therefore, the presence of such inhibitors in the circulating blood can only be assessed by the evaluation of undiluted plasma. The findings suggest that the experimental conditions which have been used in the past to demonstrate such inhibitors in the circulating blood of patients with increased susceptibility to infections are inadequate, and results need reexamination. The inhibitors affect random locomotion and chemotaxis of neutrophil granulocytes but not phagocytosis or the metabolic burst resulting in nitroblue—tetrazolium reduction. On the other hand, phagocytosis of Staphylococcus albus rendered neutrophils chemotactically unresponsive. The significance of so-called cellular defects of neutrophil chemotaxis in such patients is also considered.

Surface tension measurement of normal human blood samples by pendant drop method

2020 ◽  
Vol 44 (5) ◽  
pp. 227-236 ◽  
Author(s):  
Siddharth Singh Yadav ◽  
Basant Singh Sikarwar ◽  
Priya Ranjan ◽  
Rajiv Janardhanan ◽  
Ayush Goyal
Keyword(s):  
Surface Tension ◽  
Human Blood ◽  
Surface Tension Measurement ◽  
Pendant Drop ◽  
Blood Samples ◽  
Drop Method ◽  
Normal Human ◽  
Human Blood Samples ◽  
Pendant Drop Method ◽  
Normal Human Blood

scholarly journals The biochemical basis of nitroblue tetrazolium reduction in normal human and chronic granulomatous disease polymorphonuclear leukocytes

Blood ◽  
1976 ◽  
Vol 48 (2) ◽  
pp. 309-313 ◽  
Author(s):  
RL Baehner ◽  
LA Boxer ◽  
J Davis
Keyword(s):  
Chronic Granulomatous Disease ◽  
Polymorphonuclear Leukocytes ◽  
Nitroblue Tetrazolium ◽  
Granulomatous Disease ◽  
Biochemical Basis ◽  
Nitroblue Tetrazolium Reduction ◽  
Normal Human ◽  
Nbt Reduction ◽  
Human Polymorphonuclear Leukocytes ◽  
Tetrazolium Reduction

Abstract Normal human polymorphonuclear leukocytes (PMN) placed in anaerobic chambers reaching pO2's of less than 5 mm Hg fail to generate O2-, iodinate ingested particles, and stimulate glucose-1–14C oxidation through the hexose monophosphate shunt. The observation that anaerobic cells are incapable of generating O2- or reducing nitroblue tetrazolium (NBT) to formazan supports the idea that NBT reduction in phagocytizing PMN is due exclusively to oxygen-dependent O2- generating oxidase which is deficient in chronic granulomatous disease leukocytes, despite their hyperphagocytic capacity.

Monoamine Oxidase and Other Mitochondrial Enzymes in Density Subpopulations of Human Platelets

1988 ◽  
Vol 59 (01) ◽  
pp. 029-033 ◽  
Author(s):  
K G Chamberlain ◽  
D G Penington
Keyword(s):  
Monoamine Oxidase ◽  
Protein Concentration ◽  
Close Correlation ◽  
Human Platelets ◽  
Mitochondrial Enzymes ◽  
Density Fractions ◽  
Percoll Gradients ◽  
Normal Human ◽  
The Mean ◽  
Very High

SummaryNormal human platelets have been separated according to density on continuous Percoll gradients and the platelet distribution divided into five fractions containing approximately equal numbers of platelets. The mean volumes and protein contents of the platelets in each fraction were found to correlate positively with density while the protein concentration did not differ significantly between the fractions. Four mitochondrial enzymes (monoamine oxidase, glutamate dehydrogenase, cytochrome oxidase and NADP-dependent isocitrate dehydrogenase) were assayed and their activities per unit volume were found to increase in a very similar monotonie fashion with platelet density. When MAO and GDH were assayed on the same set of density fractions the correlation between the two activities was very high (r = 0.94–1.00, p <0.001) and a similar close correlation was found between MAO and ICDH. The results support the hypothesis that high density platelets either have a higher concentration of mitochondria or have larger mitochondria than low density platelets.

Oxygen Consumption in Platelets of Newborn Infants before and after Stimulation by Thrombin.

1976 ◽  
Vol 35 (03) ◽  
pp. 712-716 ◽  
Author(s):  
D. Del Principe ◽  
G Mancuso ◽  
A Menichelli ◽  
G Maretto ◽  
G Sabetta
Keyword(s):  
Oxygen Consumption ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Newborn Infant ◽  
Umbilical Cord Blood ◽  
Newborn Infants ◽  
O2 Consumption ◽  
Adult Control ◽  
Healthy Newborn ◽  
Before And After

SummaryThe authors compared the oxygen consumption in platelets from the umbilical cord blood of 36 healthy newborn infants with that of 27 adult subjects, before and after thrombin addition (1.67 U/ml). Oxygen consumption at rest was 6 mμmol/109/min in adult control platelets and 5.26 in newborn infants. The burst in oxygen consumption after thrombin addition was 26.30 mμmol/109/min in adults and 24.90 in infants. Dinitrophenol did not inhibit the burst of O2 consumption in platelets in 8 out of 10 newborn infants, while the same concentration caused a decrease in 9 out of 10 adult subjects. Deoxyglucose inhibited the burst in O2 consumption in newborn infant and adult platelets by about 50%. KCN at the concentration of 10−4 M completely inhibited basal oxygen consumption but did not completely inhibit the burst after thrombin. At the concentration of 10−3 M, it inhibited both basal O2 consumption and the burst in infants and adult subjects.

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