scholarly journals Bone marrow donor registries: the relation between registry size and probability of finding complete and partial matches [see comments]

Blood ◽  
1989 ◽  
Vol 74 (7) ◽  
pp. 2569-2578 ◽  
Author(s):  
FA Sonnenberg ◽  
MH Eckman ◽  
SG Pauker
Keyword(s):  
Bone Marrow ◽  
Human Leukocyte ◽  
Haplotype Frequency ◽  
Cost Effective ◽  
Frequency Data ◽  
Maximum Probability ◽  
Donor Pool ◽  
Leukocyte Antigen ◽  
Middle Range

Abstract In a registry of volunteer bone marrow donors, the relation between registry size and probability of finding an exact or partial match for a random recipient cannot be theoretically derived because it depends on specifics of the human leukocyte antigen (HLA) haplotype frequencies in the donor and recipient populations. The relation must be explicitly calculated using empirically determined HLA haplotype frequency data for all possible pairings between a donor and a recipient population. This report describes a general solution to this problem. The method shows that the relation of the probability of matching to registry size is sigmoidal, with small increases in probability at the extremes of registry size and a middle range of registry size within which the probability of matching increases most sharply. This range determines the approximate size of the most cost-effective registry. In addition, for any pairing of donor and recipient populations, there is a maximum probability of identifying a match of a given quality for a random recipient, which cannot be exceeded even if registry size were infinite. This upper limit is a function of the frequency of blank (or unknown) alleles in the donor and recipient populations; the higher that frequency, the lower the maximum probability of achieving any given quality of match. The determinants of the probability of achieving a given quality of match with a given registry size are (1) the genetic heterogeneity within the recipient and donor populations, which increases the registry size required to achieve a given probability of matching, and (2) the degree of genetic homology between the donor and recipient populations, which increases the maximum probability of matching and also lowers registry size requirements. The method described here can be used to estimate donor pool size requirements using any donor and recipient populations for which HLA frequency data are available.

scholarly journals Bone marrow donor registries: the relation between registry size and probability of finding complete and partial matches [see comments]

Blood ◽  
1989 ◽  
Vol 74 (7) ◽  
pp. 2569-2578
Author(s):  
FA Sonnenberg ◽  
MH Eckman ◽  
SG Pauker
Keyword(s):  
Bone Marrow ◽  
Human Leukocyte ◽  
Haplotype Frequency ◽  
Cost Effective ◽  
Frequency Data ◽  
Maximum Probability ◽  
Donor Pool ◽  
Leukocyte Antigen ◽  
Middle Range

In a registry of volunteer bone marrow donors, the relation between registry size and probability of finding an exact or partial match for a random recipient cannot be theoretically derived because it depends on specifics of the human leukocyte antigen (HLA) haplotype frequencies in the donor and recipient populations. The relation must be explicitly calculated using empirically determined HLA haplotype frequency data for all possible pairings between a donor and a recipient population. This report describes a general solution to this problem. The method shows that the relation of the probability of matching to registry size is sigmoidal, with small increases in probability at the extremes of registry size and a middle range of registry size within which the probability of matching increases most sharply. This range determines the approximate size of the most cost-effective registry. In addition, for any pairing of donor and recipient populations, there is a maximum probability of identifying a match of a given quality for a random recipient, which cannot be exceeded even if registry size were infinite. This upper limit is a function of the frequency of blank (or unknown) alleles in the donor and recipient populations; the higher that frequency, the lower the maximum probability of achieving any given quality of match. The determinants of the probability of achieving a given quality of match with a given registry size are (1) the genetic heterogeneity within the recipient and donor populations, which increases the registry size required to achieve a given probability of matching, and (2) the degree of genetic homology between the donor and recipient populations, which increases the maximum probability of matching and also lowers registry size requirements. The method described here can be used to estimate donor pool size requirements using any donor and recipient populations for which HLA frequency data are available.

scholarly journals Genetic diversity among volunteer donors of bone marrow in southeastern Brazil, according to the HLA system

2014 ◽  
Vol 132 (3) ◽  
pp. 158-162
Author(s):  
Letícia Sarni Roque ◽  
Rodolpho Telarolli-Junior ◽  
Leonor Castro Monteiro Loffredo
Keyword(s):  
Bone Marrow ◽  
Human Leukocyte ◽  
Mixed Race ◽  
University Hospital ◽  
Southeastern Brazil ◽  
Leukocyte Antigen ◽  
Hla System ◽  
History Of ◽  
Allelic Group ◽  
Southeastern Region

CONTEXT AND OBJECTIVE: Checking the histocompatibility of the molecules of the human leukocyte antigen (HLA) system is vital for performing bone marrow transplantation with allogeneic material. The objective of this study was to characterize bone marrow donors according to gender, age, ethnicity and HLA groups at a regional hemotherapy center in Brazil.DESIGN AND SETTING:Descriptive study on registered donors at a regional hemotherapy center in a public university hospital in the southeastern region of Brazil.METHODS: The records of 66,780 donors who were registered between 2005 and June 2011 were consulted, and the variables studied were tabulated.RESULTS:There were equal numbers of male and female donors and 82.8% of them were under 45 years of age. In terms of ethnicity, 77.3% declared themselves to be white, 15.0% mixed race, 5.7% black and 2% others. In terms of immunogenetic characterization, the most frequent HLA-A allelic group was HLA-A*02, with 39.20% of the donors; in the HLA-B allelic group, the most common was HLA-B*35, with 14.18%; while in the HLA-DRB1 allelic group, the most frequent was HLA-DRB1*03, with 17.03%. Comparison between these results and data from the Brazilian Bone Marrow Donor Registry (REDOME) showed that there were demographic and immunogenetic differences due to the history of immigration in the region of Ribeirão Preto, in southeastern Brazil.CONCLUSIONS: The results reinforce the importance of understanding the demographic and immunogenic profile of regions of Brazil, in order to reduce the waiting time for a histocompatible donor.

scholarly journals A path to renal transplantation in Nepal

1970 ◽  
Vol 1 (1) ◽  
pp. 52-55
Author(s):  
J Enns ◽  
G Aryal
Keyword(s):  
Renal Transplantation ◽  
Renal Replacement Therapy ◽  
Human Leukocyte Antigen ◽  
Replacement Therapy ◽  
Human Leukocyte ◽  
Cost Effective ◽  
Leukocyte Antigen ◽  
Post Transplant ◽  
Stage Renal Disease ◽  
End Stage

End Stage Renal Disease affects many people in the world. There are three methods of renal replacement therapy available to patients: Continuous ambulatory peritoneal dialysis, haemodialysis and transplantation. Transplantation is the most viable and cost effective form of renal replacement therapy that is available for these patients. There are 3 factors required to help ensure a successful renal transplantation program: A well legislated donor and recipient program, Human Leukocyte Antigen testing (pre and post transplant), as well as a post transplant follow up program. Keywords: Renal Transplant; South Asia; Nepal; Human Leukocyte Antigen DOI: 10.3126/jpn.v1i1.4453 Journal of Pathology of Nepal (2011) Vol.1, 52-55

Limited engraftment capacity of bone marrow–derived mesenchymal cells following T-cell–depleted hematopoietic stem cell transplantation

Blood ◽  
2000 ◽  
Vol 96 (10) ◽  
pp. 3637-3643 ◽  
Author(s):  
Daniela Cilloni ◽  
Carmelo Carlo-Stella ◽  
Franca Falzetti ◽  
Gabriella Sammarelli ◽  
Ester Regazzi ◽  
...  
Keyword(s):  
Bone Marrow ◽  
T Cell ◽  
Stromal Cells ◽  
Human Leukocyte ◽  
Mesenchymal Cells ◽  
Mixed Chimerism ◽  
Pcr Analysis ◽  
Hematopoietic Stem ◽  
Leukocyte Antigen

The engraftment capacity of bone marrow–derived mesenchymal cells was investigated in 41 patients who had received a sex-mismatched, T-cell–depleted allograft from human leukocyte antigen (HLA)–matched or –mismatched family donors. Polymerase chain reaction (PCR) analysis of the human androgen receptor (HUMARA) or the amelogenin genes was used to detect donor-derived mesenchymal cells. Only 14 marrow samples (34%) from 41 consenting patients generated a marrow stromal layer adequate for PCR analysis. Monocyte-macrophage contamination of marrow stromal layers was reduced below the levels of sensitivity of HUMARA and amelogenin assays (5% and 3%, respectively) by repeated trypsinizations and treatment with the leucyl-leucine (leu-leu) methyl ester. Patients who received allografts from 12 female donors were analyzed by means of the HUMARA assay, and in 5 of 12 cases a partial female origin of stromal cells was demonstrated. Two patients who received allografts from male donors were analyzed by amplifying the amelogenin gene, and in both cases a partial male origin of stromal cells was shown. Fluorescent in situ hybridization analysis using a Y probe confirmed the results of PCR analysis and demonstrated in 2 cases the existence of a mixed chimerism at the stromal cell level. There was no statistical difference detected between the dose of fibroblast progenitors (colony-forming unit–F [CFU-F]) infused to patients with donor- or host-derived stromal cells (1.18 ± 0.13 × 104/kg vs 1.19 ± 0.19 × 104/kg; P ≥ .97). In conclusion, marrow stromal progenitors reinfused in patients receiving a T-cell–depleted allograft have a limited capacity of reconstituting marrow mesenchymal cells.

scholarly journals A Single-Chain Fv Diabody against Human Leukocyte Antigen-A Molecules Specifically Induces Myeloma Cell Death in the Bone Marrow Environment

2007 ◽  
Vol 67 (3) ◽  
pp. 1184-1192 ◽  
Author(s):  
Etsuko Sekimoto ◽  
Shuji Ozaki ◽  
Takashi Ohshima ◽  
Hironobu Shibata ◽  
Toshihiro Hashimoto ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Cell Death ◽  
Human Leukocyte Antigen ◽  
Human Leukocyte ◽  
Myeloma Cell ◽  
Single Chain ◽  
Leukocyte Antigen ◽  
Single Chain Fv ◽  
Antigen A
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