scholarly journals Two-way cell traffic between mother and fetus: biologic and clinical implications

Blood ◽  
1996 ◽  
Vol 88 (11) ◽  
pp. 4390-4395 ◽  
Author(s):  
YM Lo ◽  
ES Lo ◽  
N Watson ◽  
L Noakes ◽  
IL Sargent ◽  
...  
Keyword(s):  
Cord Blood ◽  
Cell Trafficking ◽  
Infectious Agents ◽  
Beta Globin ◽  
Glutathione S Transferase ◽  
Maternal Circulation ◽  
Blood Samples ◽  
Fetal Cells ◽  
Maternal Peripheral Blood ◽  
Polymerase Chain

Abstract The bilateral trafficking of nucleated cells between the fetus and the mother was studied using polymerase chain reaction (PCR)-based systems sensitive enough to detect 1 target cell in 100,000 background cells. Sixty-six mother-baby pairs were recruited; maternal and cord blood samples were collected at delivery for DNA extraction. Cell trafficking was studied in informative cases using PCR-genotyping of polymorphic regions in the beta-globin cluster, the glutathione S-transferase M1 locus and the angiotensin converting enzyme gene. In addition, Y-PCR was also used in conjunction with these systems for the detection of fetal cells in maternal circulation. Fetal cells were detected in maternal peripheral blood in 26 of 51 cases whereas maternal cells were detected in 16 of 38 fetal umbilical cord blood samples. The proportion of umbilical samples with detectable maternal sequences was much higher than previously reported. In the 28 cases informative for both mother and baby, there was no obvious correlation between the cell traffic from mother to baby as compared to that from baby to mother. These findings may have implications for the use of cord blood for bone marrow transplantation, the vertical transmission of infectious agents, and the physiology of the feto-maternal relationship.

scholarly journals Two-way cell traffic between mother and fetus: biologic and clinical implications

Blood ◽  
1996 ◽  
Vol 88 (11) ◽  
pp. 4390-4395 ◽  
Author(s):  
YM Lo ◽  
ES Lo ◽  
N Watson ◽  
L Noakes ◽  
IL Sargent ◽  
...  
Keyword(s):  
Cord Blood ◽  
Cell Trafficking ◽  
Infectious Agents ◽  
Beta Globin ◽  
Glutathione S Transferase ◽  
Maternal Circulation ◽  
Blood Samples ◽  
Fetal Cells ◽  
Maternal Peripheral Blood ◽  
Polymerase Chain

The bilateral trafficking of nucleated cells between the fetus and the mother was studied using polymerase chain reaction (PCR)-based systems sensitive enough to detect 1 target cell in 100,000 background cells. Sixty-six mother-baby pairs were recruited; maternal and cord blood samples were collected at delivery for DNA extraction. Cell trafficking was studied in informative cases using PCR-genotyping of polymorphic regions in the beta-globin cluster, the glutathione S-transferase M1 locus and the angiotensin converting enzyme gene. In addition, Y-PCR was also used in conjunction with these systems for the detection of fetal cells in maternal circulation. Fetal cells were detected in maternal peripheral blood in 26 of 51 cases whereas maternal cells were detected in 16 of 38 fetal umbilical cord blood samples. The proportion of umbilical samples with detectable maternal sequences was much higher than previously reported. In the 28 cases informative for both mother and baby, there was no obvious correlation between the cell traffic from mother to baby as compared to that from baby to mother. These findings may have implications for the use of cord blood for bone marrow transplantation, the vertical transmission of infectious agents, and the physiology of the feto-maternal relationship.

Fetal cells in the maternal circulation: isolation by multiparameter flow cytometry and confirmation by polymerase chain reaction

1991 ◽  
Vol 6 (10) ◽  
pp. 1466-1469 ◽  
Author(s):  
Stephen Wachtel ◽  
Sherman Elias ◽  
James Price ◽  
Gwendolyn Wachtel ◽  
Owen Phillips ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Polymerase Chain Reaction ◽  
Multiparameter Flow Cytometry ◽  
Chain Reaction ◽  
Maternal Circulation ◽  
Fetal Cells ◽  
Polymerase Chain

scholarly journals Prenatal diagnosis of fetal hemoglobin Lepore-Boston disease on maternal peripheral blood

Blood ◽  
1990 ◽  
Vol 75 (11) ◽  
pp. 2102-2106
Author(s):  
C Camaschella ◽  
A Alfarano ◽  
E Gottardi ◽  
M Travi ◽  
P Primignani ◽  
...  
Keyword(s):  
Prenatal Diagnosis ◽  
Fetal Hemoglobin ◽  
Maternal Blood ◽  
Beta Thalassemia ◽  
Chain Reaction ◽  
Fetal Cells ◽  
Maternal Peripheral Blood ◽  
Polymerase Chain ◽  
Traditional Approaches ◽  
First Time

Molecular diagnosis of hemoglobin (Hb) Lepore-Boston in the fetus was successfully accomplished using maternal blood as a source for fetal cells in three pregnancies at risk for beta-thalassemia/Hb Lepore disease. Taking advantage of the possibility of amplifying Lepore- specific DNA fragments by polymerase chain reaction and of families in which Hb Lepore was inherited by the paternal side, we demonstrated in two cases and excluded in one case the presence of this hemoglobinopathy in the fetus directly on maternal DNA. The diagnosis was concordant with that obtained by traditional approaches in all three cases. Our results unequivocally show that nucleated fetal cells are present in maternal blood during pregnancy, and demonstrate for the first time that prenatal diagnosis of a genetic disease may be feasible without invasive procedures.

scholarly journals Isolation and Enrichment of Circulating Fetal Cells for NIPD: An Overview

Diagnostics ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 2239
Author(s):  
Giulia Sabbatinelli ◽  
Donatella Fantasia ◽  
Chiara Palka ◽  
Elisena Morizio ◽  
Melissa Alfonsi ◽  
...  
Keyword(s):  
Prenatal Diagnosis ◽  
High Efficiency ◽  
Diagnostic Testing ◽  
Genetic Research ◽  
Clinical Genetics ◽  
Maternal Circulation ◽  
Fetal Cells ◽  
Non Invasive ◽  
Prenatal Diagnostic ◽  
Maternal Peripheral Blood

Prenatal diagnosis plays a crucial role in clinical genetics. Non-invasive prenatal diagnosis using fetal cells circulating in maternal peripheral blood has become the goal of prenatal diagnosis, to obtain complete fetal genetic information and avoid risks to mother and fetus. The development of high-efficiency separation technologies is necessary to obtain the scarce fetal cells from the maternal circulation. Over the years, multiple approaches have been applied, including choice of the ideal cell targets, different cell recovering technologies, and refined cell isolation yield procedures. In order to provide a useful tool and to give insights about limitations and advantages of the technologies available today, we review the genetic research on the creation and validation of non-invasive prenatal diagnostic testing protocols based on the rare and labile circulating fetal cells during pregnancy.

Prevalence of t(12;21)[ETV6-RUNX1]–positive cells in healthy neonates

Blood ◽  
2011 ◽  
Vol 117 (1) ◽  
pp. 186-189 ◽  
Author(s):  
Ulrik Lausten-Thomsen ◽  
Hans Ole Madsen ◽  
Therese Risom Vestergaard ◽  
Henrik Hjalgrim ◽  
Jacob Nersting ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Cord Blood ◽  
Mononuclear Cells ◽  
Lymphoblastic Leukemia ◽  
Chromosomal Translocation ◽  
Chain Reaction ◽  
Blood Samples ◽  
Specific Hybridization ◽  
Stored Mrna ◽  
Polymerase Chain

Abstract t(12;21)(p13;q22)[ETV6-RUNX1] is the most common chromosomal translocation in childhood acute lymphoblastic leukemia, and it can often be backtracked to Guthrie cards supporting prenatal initiation and high levels of circulating t(12;21)-positive cells at birth. To explore the prevalence of ETV6-RUNX1–positive cells in healthy neonates, mononuclear cells from 1417 umbilical cord blood samples were isolated within 24 hours from birth and subsequently screened for ETV6-RUNX1 transcripts using a highly sensitive real-time reverse transcription polymerase chain reaction assay. In first-run polymerase chain reaction, 14 samples were positive at levels below 10−5, of which specific hybridization reflecting the relevant genetic region was positive in 9 cases. Repeated analyses using stored mRNA and flowcytometric sorting of a CD19+, CD8+, and CD19−/CD8− subpopulations from cryopreserved mononuclear cells from the same cord blood samples (mean sorted: 18 × 106 cells) revealed no positive findings, which demonstrates that the level and/or frequency of ETV6-RUNX1–positive cells is markedly lower than suggested in previous studies.

scholarly journals Prenatal diagnosis of fetal hemoglobin Lepore-Boston disease on maternal peripheral blood

Blood ◽  
1990 ◽  
Vol 75 (11) ◽  
pp. 2102-2106 ◽  
Author(s):  
C Camaschella ◽  
A Alfarano ◽  
E Gottardi ◽  
M Travi ◽  
P Primignani ◽  
...  
Keyword(s):  
Prenatal Diagnosis ◽  
Fetal Hemoglobin ◽  
Maternal Blood ◽  
Beta Thalassemia ◽  
Chain Reaction ◽  
Fetal Cells ◽  
Maternal Peripheral Blood ◽  
Polymerase Chain ◽  
Traditional Approaches ◽  
First Time

Abstract Molecular diagnosis of hemoglobin (Hb) Lepore-Boston in the fetus was successfully accomplished using maternal blood as a source for fetal cells in three pregnancies at risk for beta-thalassemia/Hb Lepore disease. Taking advantage of the possibility of amplifying Lepore- specific DNA fragments by polymerase chain reaction and of families in which Hb Lepore was inherited by the paternal side, we demonstrated in two cases and excluded in one case the presence of this hemoglobinopathy in the fetus directly on maternal DNA. The diagnosis was concordant with that obtained by traditional approaches in all three cases. Our results unequivocally show that nucleated fetal cells are present in maternal blood during pregnancy, and demonstrate for the first time that prenatal diagnosis of a genetic disease may be feasible without invasive procedures.

Exposure of the Gulf of St. Lawrence grey seal Halichoerus grypus population to potentially zoonotic infectious agents

2020 ◽  
Vol 142 ◽  
pp. 105-118
Author(s):  
CC Sauvé ◽  
A Hernández-Ortiz ◽  
E Jenkins ◽  
F Mavrot ◽  
A Schneider ◽  
...  
Keyword(s):  
Meat Products ◽  
Halichoerus Grypus ◽  
Human Consumption ◽  
Infectious Agents ◽  
Erysipelothrix Rhusiopathiae ◽  
Public Health Significance ◽  
Domestic Livestock ◽  
Health Significance ◽  
Commercial Source ◽  
Polymerase Chain

The population of grey seals Halichoerus grypus in Canadian waters is currently used as a commercial source of meat for human consumption. As with domestic livestock, it is important to understand the occurrence in these seals of infectious agents that may be of public health significance and thus ensure appropriate measures are in place to avoid zoonotic transmission. This study examined the prevalence of antibodies against Brucella spp., Erysipelothrix rhusiopathiae, 6 serovars of Leptospira interrogans, and Toxoplasma gondii in 59 grey seals and determined by polymerase chain reaction (PCR) the presence of these potentially zoonotic agents in specific organs and tissues of seropositive animals. The presence of encysted Trichinella spp. larvae was also investigated by digestion of tongue, diaphragm and other muscle samples, but none were detected. Seroprevalence against Brucella spp. and E. rhusiopathiae was low (5 and 3%, respectively). All 59 seals tested had antibodies against L. interrogans, but no carrier of this bacterium was detected by PCR. Seroprevalence against T. gondii was 53%, and DNA of this protozoan was detected by PCR in 11/30 (37%) seropositive animals. Standard sanitary measures mandatory for commercialization of meat products for human consumption should greatly reduce the potential for exposure to these infectious agents. However, special consideration should be given to freezing seal meat for at least 3 d to ensure destruction of tissue cysts of T. gondii.

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