The level of expression of anti-inflammatory cytokines NF-kB, IL-6, IFN-y, Caspasa-3, FC in chickens in the lungs and intestines during the modeling of infectious bronchitis in chickens was studied. To simulate coronavirus pneumonia, the vaccine was administered individually, 10 doses per head orally. The chickens of the 1st experimental group were fed with the Lyumantse preparation at the rate of 3 kg / t of feed, the 2nd experimental group received the Glitsevir drug at the rate of 200 μg / 0.3 ml per head. The chickens of the control group did not receive the preparations. It was revealed that antiviral drugs in the experimental groups suppressed the destruction of epithelial cells in the intestine. This may not always be an indication of a positive character, as in the case of apoptosis, not only the intestinal cells affected by the virus particles but also healthy cells are destroyed. There was a decrease in the number of active macrophages in the intestines of the experimental groups relative to the control. The amount of interferon produced was also below the control, which indicates a decreased activity of the immune system. A higher pro-inflammatory activity in the respiratory system of chickens was detected when Glicevir was used. It consists of increased expression of IL-6, interferon-gamma, macrophage receptor to Fc antibody fragments and inflammatory regulatory factor NF-kB genes compared to Lumantse with anti-inflammatory activity, but also compared to untreated control group chickens. It is concluded that it is possible to predict the risk of an exacerbation of an infectious process in the lungs against the background of a local decrease in the viral load in the intestine. An integrated approach is needed in the treatment of coronavirus infections, including either systemic antiviral drugs or anti-inflammatory drugs.
Reinforcing the Supply Chain of Umifenovir and Other Antiviral Drugs with Retrosynthetic Software
