PCR melting profile (PCR MP) - a new tool for differentiation of Candida albicansstrains

In order to identify the source of infections caused by dermatophytes, as well as the pathogen transmission pathway, there is a need to determine methods that allow detailed genetic differentiation of the strains within the dermatophyte genera. In this work, a PCR melting profile (PCR-MP) technique based on the ligation of adaptors and the difference in melting temperatures of DNA restriction fragments was used for the first time for intraspecies genotyping of dermatophytes. Clinical isolates and reference strains of dermatophytes isolated from skin, scalp, toenails and fingernails were used for this study. PCR-MP and random amplification of polymorphic DNA (RAPD) were used to type 11 isolates of Trichophyton rubrum, 40 isolates of Trichophyton interdigitale and 14 isolates of Microsporum canis. The results distinguished five types (containing one subtype) characteristic for T. rubrum and seven types characteristic for T. interdigitale using the PCR-MP technique. Analysis conducted using RAPD revealed five types for T. rubrum and four types for T. interdigitale isolates. No differentiation was observed for the M. canis isolates with either method. These results demonstrate that PCR-MP is a reliable method for the differentiation of T. rubrum and T. interdigitale strains and yields a discriminatory power that is at least equal to that of RAPD.

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Application of Microsatellite-Primed PCR (MSP-PCR) and PCR Melting Profile (PCR-MP) Method for Intraspecies Differentiation of Dermatophytes

Polish Journal of Microbiology ◽

10.33073/pjm-2014-038 ◽

2014 ◽

Vol 63 (3) ◽

pp. 283-290 ◽

Cited By ~ 3

Author(s):

ANITA CIESIELSKA ◽

MAGDALENA KOZŁOWSKA ◽

MAREK GADZALSKI ◽

MARIUSZ WOREK ◽

ADAM JAWORSKI ◽

...

Keyword(s):

Trichophyton Rubrum ◽

Microsporum Canis ◽

Melting Profile ◽

Trichophyton Interdigitale ◽

Mp Method ◽

Method Analysis ◽

Pcr Mp

In this study, two PCR-based methods (MSP-PCR and PCR-MP) were compared for their abilities to identify intraspecies variations of 23 isolates of Trichophyton rubrum, 78 isolates of Trichophyton interdigitale and 22 isolates of Microsporum canis, obtained mainly from patients in Lódź city. The results allowed to distinguish four types (containing two subtypes) characteristic for T. interdigitale and three types characteristic for T. rubrum using PCR-MP method. Analysis conducted using MSP-PCR with (GACA)4 primer revealed four types for T. rubrum and three types (containing one subtype) for T. interdigitale and with (GTG), primer showed two types (containing one subtype) for T. rubrum and six types (containing one subtype) for T. interdigitale. No differentiation was observed for the M. canis isolates with either method.

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Direct Profiling of Environmental Microbial Populations by Thermal Dissociation Analysis of Native rRNAs Hybridized to Oligonucleotide Microarrays

Applied and Environmental Microbiology ◽

10.1128/aem.69.4.2377-2382.2003 ◽

2003 ◽

Vol 69 (4) ◽

pp. 2377-2382 ◽

Cited By ~ 87

Author(s):

Said El Fantroussi ◽

Hidetoshi Urakawa ◽

Anne E. Bernhard ◽

John J. Kelly ◽

Peter A. Noble ◽

...

Keyword(s):

Thermal Dissociation ◽

Pcr Amplification ◽

Estuarine Sediments ◽

Microbial Populations ◽

Oligonucleotide Microarrays ◽

Dissociation Curve ◽

Melting Profile ◽

Probe Target ◽

Study Sites

ABSTRACT Oligonucleotide microarrays were used to profile directly extracted rRNA from environmental microbial populations without PCR amplification. In our initial inspection of two distinct estuarine study sites, the hybridization patterns were reproducible and varied between estuarine sediments of differing salinities. The determination of a thermal dissociation curve (i.e., melting profile) for each probe-target duplex provided information on hybridization specificity, which is essential for confirming adequate discrimination between target and nontarget sequences.

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Genes Leave Their Characteristic Marks on the Melting Profile of Plasmid DNAs

Zeitschrift für Naturforschung C ◽

10.1515/znc-1995-9-1008 ◽

1995 ◽

Vol 50 (9-10) ◽

pp. 652-655 ◽

Cited By ~ 2

Author(s):

Igor Z. Zubrzycki ◽

Horst H. Klump

Keyword(s):

High Resolution ◽

Resistance Gene ◽

High Resolution Melting ◽

Uv Absorbance ◽

Melting Profile ◽

Plasmid Dnas ◽

Parental Plasmid

Abstract High resolution melting profiles of four linearised plasmids (pUC9, pGV403, pHP2, and pBR322) were recorded by means of UV absorbance vs. teperature scaning. The set of transitions obtained for each plasmid are compared to each another and to the transitions obtained for their excised particular antibiotica resistance gene. It can be shown that each gene leaves a charactersitic mark on the melting profile of its parental plasmid

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