Identification of Pns6, a putative movement protein of RRSV, as a silencing suppressor

Apple chlorotic leaf spot virus (ACLSV) is the type species of the genus Trichovirus and its single-stranded, plus-sense RNA genome encodes a 216 kDa protein (P216) involved in replication, a 50 kDa movement protein (P50) and a 21 kDa coat protein (CP). In this study, it was investigated whether these proteins might have RNA silencing-suppressor activities by Agrobacterium-mediated transient assay in the green fluorescent protein-expressing Nicotiana benthamiana line 16c. The results indicated that none of these proteins could suppress local silencing in infiltrated leaves. However, systemic silencing in upper leaves induced by both single- and double-stranded RNA could be suppressed by P50, but not by a frame-shift mutant of P50, P216 or CP. Moreover, when P50 was expressed separately from where silencing signals were generated in a leaf, systemic silencing in upper leaves was inhibited. Collectively, our data indicate that P50 acts as a suppressor of systemic silencing without interfering with local silencing, probably by inhibiting the movement of silencing signals.

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RNA3 nucleotide sequence analyses ofpeanut stunt virusMi strain

Chinese Journal of Agricultural Biotechnology ◽

10.1079/cjb200552 ◽

2005 ◽

Vol 2 (1) ◽

pp. 33-38

Author(s):

Xu Ze-Yong ◽

Yan Li-Ying ◽

Chen Kun-Rong ◽

Marcel Prins

Keyword(s):

Nucleotide Sequence ◽

Movement Protein ◽

Amino Acid Identity ◽

Open Reading Frames ◽

Sequence Analyses ◽

Tomato Aspermy Virus ◽

Subgroup Ii ◽

Putative Movement Protein ◽

Peanut Stunt Virus ◽

Reading Frames

AbstractNucleotide sequence of full-length cDNA ofpeanut stunt virus(PSV) Mi strain RNA3 was determined and compared with those of PSV-ER and -J (subgroup I) and PSV-W (subgroup II), strains ofcucumber mosaic virus(CMV) andtomato aspermy virus(TAV). PSV-Mi RNA3 consists of 2170 nt and has two open reading frames, encoding a putative movement protein (3a protein) and a coat protein (CP). PSV-Mi RNA3 is 77.7% and 78.5% identical to those of PSV-ER and -J, whereas it shares 76.6% identity with PSV-W. Nucleotide identity of3aandcpgenes between PSV strains Mi and ER, J and W was 78.3–79.3% and 74.4–77.8%, respectively. Amino acid identity of 3a and CP between PSV-Mi and -ER, -J and -W was 73.9–77.4% and 64.8–77.5%, respectively. RNA3 of PSV-Mi (GenBank accession no. AY775057) had a varied intercistronic and 5′-untranslated region compared with those of PSV strains ER, J and W. Results indicate that PSV-Mi represents a new PSV subgroup from China, designated as subgroup III.

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Pineapple bacilliform CO virus: Diversity, Detection, Distribution, and Transmission

Plant Disease ◽

10.1094/pdis-08-11-0718-re ◽

2012 ◽

Vol 96 (12) ◽

pp. 1798-1804 ◽

Cited By ~ 13

Author(s):

D. M. Sether ◽

M. J. Melzer ◽

W. B. Borth ◽

J. S. Hu

Keyword(s):

Reverse Transcriptase ◽

Movement Protein ◽

Rnase H ◽

Open Reading Frames ◽

Ananas Comosus ◽

Genomic Variants ◽

Virus Diversity ◽

Putative Movement Protein ◽

Dysmicoccus Neobrevipes ◽

Reading Frames

Members of the genus Badnavirus (family Caulimovirdae) have been identified in dicots and monocots worldwide. The genome of a pineapple badnavirus, designated Pineapple bacilliform CO virus-HI1 (PBCOV-HI1), and nine genomic variants (A through H) were isolated and sequenced from pineapple, Ananas comosus, in Hawaii. The 7,451-nucleotide genome of PBCOV-HI1 possesses three open reading frames (ORFs) encoding putative proteins of 20 (ORF1), 15 (ORF2), and 211 (ORF3) kDa. ORF3 encodes a polyprotein that includes a putative movement protein and viral aspartyl proteinase, reverse transcriptase, and RNase H regions. Three distinct groups of putative endogenous pineapple pararetroviral sequences and Metaviridae-like retrotransposons encoding long terminal repeat, reverse-transcriptase, RNase H, and integrase regions were also identified from the pineapple genome. Detection assays were developed to distinguish PBCOV-HI1 and genomic variants, putative endogenous pararetrovirus sequences, and Ananas Metaviridae sequences also identified in pineapple. PBCOV-HI1 incidences in two commercially grown pineapple hybrids, PRI 73-114 and PRI 73-50, was 34 to 68%. PBCOV-HI1 was transmitted by gray pineapple mealybugs, Dysmicoccus neobrevipes, to pineapple.

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In VivoDetection, RNA-Binding Properties and Characterization of the RNA-Binding Domain of the p7 Putative Movement Protein from Carnation Mottle Carmovirus (CarMV)

Virology ◽

10.1006/viro.1998.9596 ◽

1999 ◽

Vol 255 (2) ◽

pp. 354-365 ◽

Cited By ~ 48

Author(s):

Jose F. Marcos ◽

Marçal Vilar ◽

Enrique Pérez-Payá ◽

Vicente Pallás

Keyword(s):

Rna Binding ◽

Movement Protein ◽

Binding Domain ◽

Binding Properties ◽

Properties And Characterization ◽

Putative Movement Protein ◽

Rna Binding Domain

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Subcellular localization and in vivo identification of the putative movement protein of olive latent virus 2.

Journal of General Virology ◽

10.1099/0022-1317-80-5-1103 ◽

1999 ◽

Vol 80 (5) ◽

pp. 1103-1109 ◽

Cited By ~ 27

Author(s):

F Grieco ◽

M A Castellano ◽

G P Di Sansebastiano ◽

G Maggipinto ◽

J M Neuhaus ◽

...

Keyword(s):

Subcellular Localization ◽

Movement Protein ◽

Latent Virus ◽

Putative Movement Protein

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Serological Detection of Grapevine Virus A Using Antiserum to a Nonstructural Protein, the Putative Movement Protein

Phytopathology ◽

10.1094/phyto.1997.87.10.1041 ◽

1997 ◽

Vol 87 (10) ◽

pp. 1041-1045 ◽

Cited By ~ 29

Author(s):

E. Rubinson ◽

N. Galiakparov ◽

S. Radian ◽

I. Sela ◽

E. Tanne ◽

...

Keyword(s):

Movement Protein ◽

Early Stage ◽

Nonstructural Protein ◽

Effective Means ◽

Immunoblot Analysis ◽

Enzyme Linked Immunosorbent Assay ◽

Grapevine Virus ◽

Serological Detection ◽

Grapevine Virus A ◽

Putative Movement Protein

Grapevine virus A (GVA) is implicated in the etiology of the rugose wood disease. The coat protein (CP) and the putative movement protein (MP) genes of GVA were cloned and expressed in Escherichia coli and used to produce antisera. Both the CP and the MP were detected with their corresponding antisera in GVA-infected Nicotiana benthamiana. The MP was first detected at an early stage of the infection, 6 to 12 h after inoculation, and the CP was detected 2 to 3 days after inoculation. The CP and MP were detected by immunoblot analysis in rugose wood-affected grapevines. The MP could be detected in GVA-infected grapevines that tested negative for CP, both with CP antiserum and with a commercially available enzyme-linked immunosorbent assay kit. The study shows that detection of the nonstructural MP may be an effective means for serological detection of GVA infection in grapevines.

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