scholarly journals Local cytokine transcription in naïve and previously infected sheep and lambs following challenge with Teladorsagia circumcincta

2014 ◽  
Vol 10 (1) ◽  
pp. 87 ◽  
Author(s):  
Nicola M Craig ◽  
David W Smith ◽  
Judith A Pate ◽  
Ivan W Morrison ◽  
Pamela A Knight
Keyword(s):  
Infected Sheep ◽  
Teladorsagia Circumcincta ◽  
Local Cytokine ◽  
Cytokine Transcription

scholarly journals Development and Evaluation of an Indirect ELISA for Detection of Infection with Teladorsagia Circumcincta in Sheep

2021 ◽  
Author(s):  
Jalal Aliabadi ◽  
Ehsan Rakhshandehroo ◽  
Azadeh Yektaseresht
Keyword(s):  
Indirect Elisa ◽  
High Specificity ◽  
Negative Control ◽  
Infected Sheep ◽  
Diagnostic Methods ◽  
Elisa Method ◽  
Specific Product ◽  
Molecular Weights ◽  
Teladorsagia Circumcincta ◽  
The Cross

Abstract Background The gastrointestinal helminth, Teladorsagia circumcincta, is one of the major health risk and production-limiting disease in small ruminant populations, particularly in temperate regions. With the increasing importance of the disease management and recruited anthelminic resistant types, accurate approaches are needed for diagnosis of the infection in the host. Because of uncertain results using faecal examinations, the ELISA method was indicated for detection of the nematode antigenic materials. Despite some promising results, problems were described in terms of the test specificity and the cross-reactions. The aim of this study was therefore to evaluate worm somatic and excretory/secretory (ES) products using western blot analysis and an indirect ELISA for detecting T. circumcincta infection in sheep. Results Based on the immuno-reactivity analysis, immunogenic fractions with molecular weights of approximately 60, 75 and 100 kDa were detected in somatic content and two antigens of about 63 and 75 kDa in ES material. Accordingly, a specific product at 75 kDa had the strongest reaction and appeared as the most common antigenic protein. In ELISA, all sera from the infected sheep revealed the OD rates above the calculated cut-off value with about two-fold greater in average. Negative control samples were also specifically recognized with the mean OD rate of about 1/3 of the estimated cut-off value. The cross-reaction test, using rabbit hyperimmune sera, did not show reactivity with ES antigens of other prevalent nematodes include Haemonchus contortus, Protostrongylus rufescens and Marshallagia marshalli. In contrast, a strong positive reaction was found with the somatic antigens of M. marshalli. Conclusions The results obtained here indicates the indirect ELISA method using the ES content enables distinguishing the T. circumcincta infected sheep with high specificity. Those antigenic ES peptides with 63 and particularly 75 kDa molecular weights should be more investigated due to the potential for serological diagnostic methods and immunoprotective targets in the host.

scholarly journals Development and evaluation of an indirect ELISA for detection of Teladorsagia circumcincta infection in sheep

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Jalal Aliabadi ◽  
Ehsan Rakhshandehroo ◽  
Azadeh Yektaseresht
Keyword(s):  
Indirect Elisa ◽  
High Specificity ◽  
Negative Control ◽  
Infected Sheep ◽  
Diagnostic Methods ◽  
Elisa Method ◽  
Specific Product ◽  
Molecular Weights ◽  
Teladorsagia Circumcincta ◽  
Test Specificity

Abstract Background The gastrointestinal helminth, Teladorsagia circumcincta, is one of the major health risks and production-limiting diseases in small ruminant populations, particularly in temperate regions. With the increasing importance of disease management and recruited anthelmintic resistant types, accurate approaches are needed for the diagnosis of the infection in the host. Due to uncertain results using faecal examinations, the ELISA method was indicated for the detection of nematode antigenic materials. Despite some promising results, problems were described in terms of test specificity and cross-reactions. Therefore, this study aimed to evaluate the IgG response to worm somatic and excretory/secretory (ES) products using western blot analysis and an indirect ELISA for the detection of T. circumcincta infection in sheep. Results Based on the immuno-reactivity analysis, immunogenic fractions with molecular weights (MWs) of approximately 60, 75 and 100 kDa were detected in somatic content and two antigens of about 63 and 75 kDa in ES material. Accordingly, a specific product at 75 kDa had the strongest reaction and appeared as the most common antigenic protein. In ELISA, all the sera from the infected sheep revealed the OD rates above the calculated cut-off value with about two-fold greater average. Negative control samples were also specifically recognized with the mean OD rate of about 1/3 of the estimated cut-off value. The cross-reaction test, using rabbit anti-T. circumcincta IgG, did not show reactivity with the ES antigens of other prevalent nematodes including Haemonchus contortus, Protostrongylus rufescens and Marshallagia marshalli. In contrast, a strong positive reaction was observed with the somatic antigens of M. marshalli. Conclusions The results of this study indicated that the indirect ELISA method using the ES content enables distinguishing the T. circumcincta infected sheep with high specificity. Those antigenic ES peptides with 63 and particularly 75 kDa MWs should be further investigated due to the potential for serological diagnostic methods and immunoprotective targets in the host.

The genetic control of IgA activity against Teladorsagia circumcincta and its association with parasite resistance in naturally infected sheep

Parasitology ◽  
2002 ◽  
Vol 124 (5) ◽  
pp. 545-552 ◽  
Author(s):  
S. A. J. STRAIN ◽  
S. C. BISHOP ◽  
N. G. HENDERSON ◽  
A. KERR ◽  
Q. A. MCKELLAR ◽  
...  
Keyword(s):  
Genetic Control ◽  
Adult Female ◽  
Parasite Growth ◽  
Infected Sheep ◽  
Parasite Resistance ◽  
Teladorsagia Circumcincta ◽  
Growth Development ◽  
Low Levels ◽  
Parasite Challenge

Previous studies in deliberately infected sheep have shown an association between IgA activity against 4th-stage larvae of Teladorsagia circumcincta and parasite growth, development and fecundity. The purpose of this research was to determine if these results could be confirmed in naturally infected sheep and to explore the hypothesis that plasma IgA activity could help to identify resistant lambs with shorter adult nematodes. Plasma IgA activity was skewed with most animals having relatively low levels of IgA activity. Plasma IgA activity was repeatable and highly heritable. Animals with increased IgA activity had lower egg counts and shorter adult female T. circumcincta. Therefore, under conditions of natural parasite challenge, plasma IgA activity may help to identify lambs resistant to T. circumcincta.

Evaluation of the Systemic-Local Cytokine Profile in Patients with Pseudoexfoliation Syndrome

2017 ◽  
Vol 2 (3) ◽  
pp. 82-87
Author(s):  
N. Lutsenko ◽  
◽  
O. Isakova ◽  
O. Rudycheva ◽  
T. Mihalchik
Keyword(s):  
Cytokine Profile ◽  
Pseudoexfoliation Syndrome ◽  
Local Cytokine

Transplacental transmission of Neospora caninum to lambs in successive pregnancies of naturally infected sheep in Southern Brazil

2021 ◽  
Vol 23 ◽  
pp. 100537
Author(s):  
Kamila Alcalá Gonçalves Pereira ◽  
Renato Silva de Sousa ◽  
Mary Suzan Varaschin ◽  
Ana Paula Brenner Busch Becker ◽  
Alda Lúcia Gomes Monteiro ◽  
...  
Keyword(s):  
Neospora Caninum ◽  
Infected Sheep ◽  
Transplacental Transmission ◽  
Southern Brazil

scholarly journals An analysis of the transcriptome of Teladorsagia circumcincta: its biological and biotechnological implications

BMC Genomics ◽  
2012 ◽  
Vol 13 (Suppl 7) ◽  
pp. S10 ◽  
Author(s):  
Ranjeeta Menon ◽  
Robin B Gasser ◽  
Makedonka Mitreva ◽  
Shoba Ranganathan
Keyword(s):  
Teladorsagia Circumcincta

scholarly journals Evaluation of a Preclinical Blood Test for Scrapie in Sheep Using Immunocapillary Electrophoresis

2006 ◽  
Vol 89 (3) ◽  
pp. 720-727 ◽  
Author(s):  
Roy Jackman ◽  
David J Everest ◽  
Mary Jo Schmerr ◽  
Mohammed Khawaja ◽  
Pat Keep ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Prion Protein ◽  
Clinical Signs ◽  
Test System ◽  
Buffy Coat ◽  
Infected Sheep ◽  
Test Method ◽  
Peptide Sequence ◽  
Transmissible Spongiform Encephalopathies ◽  
Blood Samples

Abstract An analytical method is described for detection of endogenous disease-associated prion protein in the buffy coat fraction from the blood of sheep infected with scrapie. The method has been improved and evaluated for its performance in the preclinical diagnosis of ovine transmissible spongiform encephalopathies. The test system uses a protocol for sample preparation that includes extraction and concentration and a test method that uses a liquid-phase competitive immunoassay for prion protein. Antibodies directed to a peptide sequence at the C-terminus of the prion protein (PrP) and a fluorescein-labeled peptide conjugate are used in the assay. Free zone capillary electrophoresis with laser-induced fluorescence for detection is used to separate the antibody-bound fluorescently labeled peptide and free labeled peptide. In this assay, the PrP competes with the fluorescently labeled peptide for limited antibody binding sites, which results in a reduction of the peak representing the immunocomplex of the antibody bound to the fluorescently labeled peptide. When blood samples from scrapie-infected sheep aged 712 months and of the scrapie-susceptible PrP genotypes VRQ/VRQ and VRQ/ARQ were analyzed, the abnormal PrP was found in blood samples. These results correlated with the post-mortem diagnosis of scrapie. The sheep were preclinical and appeared normal at the time of testing but later died with clinical disease approximately 12 months after testing. In older animals, and those with clinical signs, a smaller percentage of animals tested positive. This study has demonstrated that this technology can be used as a sensitive, rapid preclinical test to detect the disease-associated PrP in the blood of scrapie-infected sheep. Improvements in the extraction protocol and capillary electrophoresis conditions will enhance the robustness of this test.

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