scholarly journals Influence of Sperm Number Per Straw on the Post-Thaw Sperm Viability and Fertility of Swedish Red and White A.I. Bulls

1996 ◽  
Vol 37 (4) ◽  
pp. 461-470
Author(s):  
A. Januskauskas ◽  
L. Söderquist ◽  
M. G. Håård ◽  
M. Ch ◽  
N. Lundeheim ◽  
...  
Keyword(s):  
Sperm Viability ◽  
Sperm Number

scholarly journals Apis mellifera (Hymenoptera: Apidae) drone sperm quality in relation to age, genetic line, and time of breeding

2015 ◽  
Vol 147 (6) ◽  
pp. 702-711 ◽  
Author(s):  
Andrée Rousseau ◽  
Valérie Fournier ◽  
Pierre Giovenazzo
Keyword(s):  
Apis Mellifera ◽  
Honey Bee ◽  
Sperm Quality ◽  
Research Centre ◽  
Sperm Viability ◽  
Sperm Number ◽  
Genetic Line ◽  
Semen Volume ◽  
Time Of Breeding ◽  
Honey Bee Queen

AbstractA honey bee (Apis mellifera Linnaeus; Hymenoptera: Apidae) queen’s life expectancy is strongly dependent on the number of sperm she obtains by mating with drones during nuptial flights. Unexplained replacement of queens by the colony and young queens showing sperm depletions have been reported in North America, and reduced drone fertility has been a suspected cause. The aim of this study was to evaluate drone reproductive qualities during the queen-rearing season, from May to August. Drones from two different genetic lines were reared six times during the 2012 beekeeping season at our research centre in Québec (Canada). Semen volume as well as sperm number and viability were assessed at the ages of 14, 21, and 35 days. Results showed (1) a greater proportion of older drones with semen at the tip of the genitalia after eversion; (2) an influence of rearing date on semen production; and (3) no influence of drone genetic line, age or time of breeding on sperm viability. These results highlight the necessity of better understanding drone rearing and how it can be improved to ensure optimum honey-bee queen mating.

Is sperm viability independent of ejaculate size in the house cricket (Acheta domesticus)?

2011 ◽  
Vol 89 (12) ◽  
pp. 1231-1236 ◽  
Author(s):  
Brian E. Gress ◽  
Clint D. Kelly
Keyword(s):  
Experimental Treatment ◽  
Acheta Domesticus ◽  
Sperm Viability ◽  
Sperm Number ◽  
Physical Damage ◽  
Viability Assay ◽  
Ejaculate Size ◽  
Sperm Counts ◽  
Spurious Results ◽  
Related Mortality

Assessing sperm viability is a popular means of testing hypotheses related to ejaculate quality. This technique has produced interesting results; however, the sperm viability assay itself may kill sperm. This is a serious pitfall, as assay-related mortality could confound results and produce artificially low estimates of viability. To avoid spurious results, it has been recommended that investigators include sperm number in their viability analyses. Unfortunately, studies conducted to date on internal fertilizers have not included sperm counts in their analyses, so it is not possible to assess whether this factor can indeed produce artefactual results. In this paper, we use male house crickets ( Acheta domesticus (L., 1758)) to show that sperm viability is dependent on sperm number and exclusion of this factor from statistical analyses affects our interpretation of experimental treatment results. We show that mechanically rupturing a spermatophore significantly reduces sperm viability, but this does not appear to drive the nonindependent relationship between viability and number. Instead, our study shows that nonindependence is due to processes other than differential physical damage to the sperm during collection. We also show that allowing a spermatophore to evacuate its sperm without rupturing for 10 min maximizes both sperm number and viability.

scholarly journals Protein and carbohydrate intake influence sperm number and fertility in male cockroaches, but not sperm viability

2015 ◽  
Vol 282 (1802) ◽  
pp. 20142144 ◽  
Author(s):  
Harriet Bunning ◽  
James Rapkin ◽  
Laurence Belcher ◽  
C. Ruth Archer ◽  
Kim Jensen ◽  
...  
Keyword(s):  
Recent Work ◽  
Male Fertility ◽  
Nutrient Intake ◽  
Sperm Viability ◽  
Sperm Number ◽  
Sperm Production ◽  
High Intake ◽  
Trade Off ◽  
Numerous Species ◽  
Dietary Choice

It is commonly assumed that because males produce many, tiny sperm, they are cheap to produce. Recent work, however, suggests that sperm production is not cost-free. If sperm are costly to produce, sperm number and/or viability should be influenced by diet, and this has been documented in numerous species. Yet few studies have examined the exact nutrients responsible for mediating these effects. Here, we quantify the effects of protein (P) and carbohydrate (C) intake on sperm number and viability in the cockroach Nauphoeta cinerea , as well as the consequences for male fertility. We found the intake of P and C influenced sperm number, being maximized at a high intake of diets with a P : C ratio of 1 : 2, but not sperm viability. The nutritional landscapes for male fertility and sperm number were closely aligned, suggesting that sperm number is the major determinant of male fertility in N. cinerea . Under dietary choice, males regulate nutrient intake at a P : C ratio of 1 : 4.95, which is midway between the ratios needed to maximize sperm production and pre-copulatory attractiveness in this species. This raises the possibility that males regulate nutrient intake to balance the trade-off between pre- and post-copulatory traits in this species.

scholarly journals Ameliorative effect of Allium atroviolaceum on sperm quality in cyclophosphamide-treated mice

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Alireza Hosseini ◽  
Mehrdad Shahrani ◽  
Shirin Asgharian ◽  
Maryam Anjomshoa ◽  
Ayoob Rostamzadeh ◽  
...  
Keyword(s):  
Side Effects ◽  
Alkylating Agent ◽  
Sperm Quality ◽  
Sperm Count ◽  
Reproductive Toxicity ◽  
Herbal Drugs ◽  
Sperm Viability ◽  
Complementary Treatment ◽  
Ameliorative Effect ◽  
Alternative Drugs

Abstract Background Cyclophosphamide (CP) is an anti-neoplastic alkylating agent that is extensively used in different chemotherapy regimens. Adverse effects on the reproductive system, especially spermatogenesis, are one of the most important side effects of this drug. It is medically essential to use complementary and alternative drugs. Herbal drugs have long been used as a complementary treatment. Our purpose was to study the effect of hydroalcoholic Allium atroviolaceum L. extract on spermatogenesis in CP-treated mice. Results CP affected a significant decrease in sperm count, motility, viability, and morphology. Sperm count was significantly higher in the all extract groups than in the group of control (p<0.001) and CP group (p<0.001, p<0.01). Sperm motility was significantly greater in the extract (100 and 200mg/kg) groups than in the group of control (p<0.05 and <0.001). Sperm immotility and rotational movement were significantly higher in the CP group than in the CP+extract groups (p<0.001). The sperm viability was significantly greater in the CP+extract (200mg/kg) group than in the CP group (p<0.001). The number of headless sperm, sperm with initial tail, with coiled tail, and sperm with curved body, was significantly lower in the CP+extract (200mg/kg) group than in the CP group (p<0.001). Conclusion A. atroviolaceum extract treatment significantly improved CP-induced reproductive toxicity.

scholarly journals Viability of Najawa carp (Cyprinus carpio L.) sperm at 4°C temperature storage

2020 ◽  
Vol 147 ◽  
pp. 01015
Author(s):  
Dimas Fendy Pradana ◽  
Ignatius Hardaningsih ◽  
Dini Wahyu Kartika Sari
Keyword(s):  
Low Temperature ◽  
Cyprinus Carpio ◽  
Sperm Viability ◽  
Low Temperature Storage ◽  
Progressive Motility ◽  
C Storage ◽  
Randomized Design ◽  
Significant Difference ◽  
Carp Cyprinus Carpio ◽  
Temperature Storage

The objectives of this study were to evaluate the sperm viability of Najawa carp (Cyprinus carpio L.) in cryopreservation pre-conditions at 4°C. The design used in this study was Complete Randomized Design with 4 treatments, BSS as a control, 10% DMSO, 0,2 M Sucrose, and 5% DMSO + 0,1 M Sucrose; each consist of three replications. The parameters observed were progressive motility of fresh sperm, diluted sperm before low temperature storage, and 2 hours; 3 hours; 4 hours; 5 hours; one day; one week; a month after 4°C storage. The data were analyzed by ANOVA. The data showed that there was no significant difference between treatment (P>0.05). The best viability was 40.56% of sperm motility which survive for one week, it was achieved by 5% DMSO + 0,1 M Sucrose.

Application of MTT reduction assay to evaluate equine sperm viability

2005 ◽  
Vol 64 (6) ◽  
pp. 1350-1356 ◽  
Author(s):  
D.M. Aziz ◽  
L. Ahlswede ◽  
H. Enbergs
Keyword(s):  
Sperm Viability ◽  
Reduction Assay ◽  
Mtt Reduction

scholarly journals Female major histocompatibility complex type affects male testosterone levels and sperm number in the horse ( Equus caballus )

2015 ◽  
Vol 282 (1807) ◽  
pp. 20150407 ◽  
Author(s):  
D. Burger ◽  
G. Dolivo ◽  
E. Marti ◽  
H. Sieme ◽  
C. Wedekind
Keyword(s):  
Major Histocompatibility Complex ◽  
Reproductive Strategies ◽  
Kin Recognition ◽  
Equus Caballus ◽  
Major Histocompatibility ◽  
Sperm Number ◽  
Sperm Velocity ◽  
Testosterone Levels ◽  
Histocompatibility Complex ◽  
Male Reproductive Strategies

Odours of vertebrates often contain information about the major histocompatibility complex (MHC), and are used in kin recognition, mate choice or female investment in pregnancy. It is, however, still unclear whether MHC-linked signals can also affect male reproductive strategies. We used horses ( Equus caballus ) to study this question under experimental conditions. Twelve stallions were individually exposed either to an unfamiliar MHC-similar mare and then to an unfamiliar MHC-dissimilar mare, or vice versa. Each exposure lasted over a period of four weeks. Peripheral blood testosterone levels were determined weekly. Three ejaculates each were collected in the week after exposure to both mares (i.e. in the ninth week) to determine mean sperm number and sperm velocity. We found high testosterone levels when stallions were kept close to MHC-dissimilar mares and significantly lower ones when kept close to MHC-similar mares. Mean sperm number per ejaculate (but not sperm velocity) was positively correlated to mean testosterone levels and also affected by the order of presentation of mares: sperm numbers were higher if MHC-dissimilar mares were presented last than if MHC-similar mares were presented last. We conclude that MHC-linked signals influence testosterone secretion and semen characteristics, two indicators of male reproductive strategies.

Melatonin and canthaxanthin enhances sperm viability and protect ram spermatozoa from oxidative stress during liquid storage at 4°C

Andrologia ◽  
2021 ◽  
Author(s):  
Shivendra Kumar Bhalothia ◽  
Jitendra Singh Mehta ◽  
Tapendra Kumar ◽  
Chandan Prakash ◽  
Thirumala Rao Talluri ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Sperm Viability ◽  
Liquid Storage
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