Melatonin and canthaxanthin enhances sperm viability and protect ram spermatozoa from oxidative stress during liquid storage at 4°C

Antioxidants are linked with sperm viability because of their protective effects against cell damage during preservation. In order to enhance the life span of refrigerated buck semen, this study was carried out to determine the effect of fruit-rich antioxidants on spermatozoa viability and lipid peroxidation (LPO) of buck semen during liquid storage. Pooled semen from five Red Sokoto bucks was diluted with Tris-egg yolk based extender and supplemented each with juices from pawpaw tomato and watermelon at 0, 2.5, 5, 7.5 and 10/ 100 ml respectively. Following dilution, the semen samples were assessed subjectively after in vitro storage at 5°C for 24, 48, 72 and 96 hours as regards sperm motility, abnormalities, and acrosome status using a phase-contrast microscope. The concentration of malondialdehyde (MDA) as indices of lipid peroxidation (LPO) in the stored semen was measured in thiobarbituric acid reactive substances (TBARS) at 24, 48, 72 and 96 hours. The results showed highest progressive motility in watermelon juice at 2.5% (P<0.05) during the first 24 hours of storage while the lowest progressive motility was recorded at various levels of pawpaw juice (P<0.05). After 48 hours of storage, extender supplemented with watermelon and tomato juices had better progressive motility compared to control except 7.5% and 10%% of tomato juice (P<0.05). Irrespective of level of juice in the extender, the percentage of intact acrosome was similar among the various juices and control. The results showed that spermatozoa extended with watermelon juice had the lowest (P<0.05) percentage abnormality compared to other extenders at 24, 48, 72 and 96 hours of storage. Higher (P<0.05) percent spermatozoa abnormality compared to other fruit juices and control was observed at 72 and 96 hours of storage in spermatozoa extended with pawpaw juice. Significant reductions of MDA concentrations were achieved by addition of fruit-rich antioxidants to Tris-egg yolk based extender during the first 72 hours and the reduction was much pronounced in extender supplemented with pawpaw juice compared to control (P<0.05). The findings reveal that fruit-rich antioxidants from watermelon and tomato have protective ability to maintain sperm viability and to reduce concentration MDA of buck semen during liquid storage.

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Influence of sperm concentration on the motility, morphology, membrane and DNA integrity along with oxidative stress parameters of ram sperm during liquid storage

Animal Reproduction Science ◽

10.1016/j.anireprosci.2010.08.012 ◽

2010 ◽

Vol 122 (3-4) ◽

pp. 200-207 ◽

Cited By ~ 26

Author(s):

M. Gundogan ◽

D. Yeni ◽

F. Avdatek ◽

A.F. Fidan

Keyword(s):

Oxidative Stress ◽

Sperm Concentration ◽

Dna Integrity ◽

Oxidative Stress Parameters ◽

Liquid Storage ◽

Ram Sperm ◽

Stress Parameters

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Effects of pH during liquid storage of goat semen on sperm viability and fertilizing potential

Animal Reproduction Science ◽

10.1016/j.anireprosci.2015.11.011 ◽

2016 ◽

Vol 164 ◽

pp. 47-56 ◽

Cited By ~ 4

Author(s):

Chang-He Liu ◽

Hai-Bo Dong ◽

Dong-Li Ma ◽

You-Wei Li ◽

Dong Han ◽

...

Keyword(s):

Sperm Viability ◽

Liquid Storage ◽

Effects Of Ph

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Sperm viability, apoptosis, and intracellular reactive oxygen species levels in human spermatozoa before and after induction of oxidative stress

Fertility and Sterility ◽

10.1016/j.fertnstert.2008.10.068 ◽

2010 ◽

Vol 93 (3) ◽

pp. 814-821 ◽

Cited By ~ 78

Author(s):

Reda Z. Mahfouz ◽

Stefan S. du Plessis ◽

Nabil Aziz ◽

Rakesh Sharma ◽

Edmund Sabanegh ◽

...

Keyword(s):

Oxidative Stress ◽

Reactive Oxygen Species ◽

Reactive Oxygen ◽

Intracellular Reactive Oxygen Species ◽

Human Spermatozoa ◽

Oxygen Species ◽

Sperm Viability ◽

Before And After

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The effect of royal jelly on boar sperm viability and motility during liquid storage for 96 hours

Acta Veterinaria Brno ◽

10.2754/avb202089010047 ◽

2020 ◽

Vol 89 (1) ◽

pp. 47-53

Author(s):

Aiste Iljenkaite ◽

Sigita Kerziene ◽

Agila Dauksiene ◽

Zoja Mikniene ◽

Henrikas Žilinskas ◽

...

Keyword(s):

Sperm Motility ◽

Storage Time ◽

Royal Jelly ◽

Incubation Temperature ◽

Ph Value ◽

Protective Effects ◽

Sperm Viability ◽

Liquid Storage ◽

Sperm Plasma Membrane ◽

Boar Semen

The current study was carried out to investigate the protective effects of royal jelly supplementation on sperm motility, viability and pH value during the liquid storage of boar semen at 16 °C and 4 °C, at various periods of time (0, 24, 48, 72 and 96 h). Semen samples were collected from 11 boars, diluted with a long-term extender and supplemented with different concentration of royal jelly (0%, 0.5%, 1% and 2%) at a final concentration of 50 × 106 sperm/ml. In the laboratory, the semen was assessed for sperm morphology, viability (eosin-nigrosin staining), subjective motility and objective sperm motility by sperm class analyzer. In total, 396 tests for sperm viability and motility were performed. The longer storage time and the lower incubation temperature showed lower sperm motility and viability results. The results showed that royal jelly supplementation at 1% concentrations protected the functionality of the sperm plasma membrane during the liquid storage time of 96 h at 16 °C. Sperm subjective and objective motility results in samples stored at 4 °C decreased with higher royal jelly concentrations and longer storage time, and differ significantly from the results in samples stored at 16 °C (P < 0.05). Our data showed that royal jelly supplementation at lower concentrations can improve boar semen motility and viability parameters during liquid storage at 16 °C for 96 h.

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269IN VITRO FERTILITY OF BOAR SPERMATOZOA PRESERVED AT 10^°C FOR 22 DAYS

Reproduction Fertility and Development ◽

10.1071/rdv16n1ab269 ◽

2004 ◽

Vol 16 (2) ◽

pp. 255

Author(s):

H. Funahashi

Keyword(s):

Oxidative Stress ◽

Functional Status ◽

Pregnancy Rate ◽

Seminal Plasma ◽

Artificial Insemination ◽

Fluorescence Assay ◽

Live Cells ◽

Sperm Viability ◽

Boar Spermatozoa

Fertility of boar spermatozoa as determined following artificial insemination seems to be maintained during liquid preservation at 10–15°C for several days, although prolonged liquid preservations reduce the pregnancy rate rapidly. However, it is not clear if spermatozoa can penetrate into oocytes in an IVF system even after a prolonged liquid preservation. Oxidative stress could also be one of the possible detrimental factors in liquid preservation of spermatozoa. In the present study, fertility of liquid-preserved spermatozoa was examined using an IVM-IVF system. Whether cysteine can improve the fertility was also determined. Spermatozoa (from four Berkshires) was resuspended at 1×108 cells mL−1 in Modena solution containing 15% (v/v) boar seminal plasma and 0 or 5mM cysteine after washing 3 times. Sperm suspensions (1mL) were then preserved at 10°C for 22 days following a program for cooling down (to 15°C for 4h, keeping at 15°C for 12h and then to 10°C for 6h). At Days 1, 8, 15 and 22 after the start of preservation, spermatozoa (5×105 cells mL−1) were co-cultured with IVM oocytes in an IVM/IVF system (Funahashi et al., 1997 Biol Reprod 57, 49–53). Viability and functional status of spermatozoa were also examined at Days 8 and 15 of preservation by using LIVE/DEAD sperm viability kit and CTC fluorescence assay. Data (mean±SEM) from 4–6 replicates were analyzed by ANOVA and Fisher’s protected LSD test. When spermatozoa that had been preserved without cysteine (Cys−) were used, penetration rates were not different (P>0.05) from those with cysteine (Cys+) at Day 8 of preservation (91.4±3.4% in Cys− and 99.3±0.7% in Cys+), but lower (P<0.02) at Days 15 and 22 (72.6±13.6% and 33.8±8.4% in Cys−; 94.8±2.1% and 71.1±10.8% in Cys+, respectively). Both viability and proportion of uncapacitated live cells were higher (P<0.05) in Cys+ than Cys− at Days 8 and 15. These results demonstrate that boar spermatozoa can penetrate into oocytes in vitro even after a liquid preservation at 10°C for 22 days and that cysteine can improve the viability and penetrability in vitro of spermatozoa during liquid preservation. Supported by the Ito Foundation.

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Positive effects of trehalose and cysteine on ram sperm parameters

Veterinární Medicína ◽

10.17221/131/2016-vetmed ◽

2017 ◽

Vol 62 (No. 5) ◽

pp. 245-252 ◽

Cited By ~ 2

Author(s):

S. Gungor ◽

C. Ozturk ◽

AD Omur

Keyword(s):

Sperm Motility ◽

Sperm Parameters ◽

Control Group ◽

Mitochondrial Activity ◽

Sperm Viability ◽

Significant Protection ◽

Positive Effects ◽

Liquid Storage ◽

Acrosome Integrity ◽

Ram Sperm

The aim of this study was to determine the effects of trehalose and cysteine on sperm motility, viability, mitochondrial activity and acrosome integrity during liquid storage of Merino ram semen. Ejaculates were collected using artificial vaginas from five Merino rams, microscopically evaluated and pooled at 37 °C. The pooled semen samples were diluted in a Tris-based extender, including cysteine (2 mM and 4 mM), trehalose (10 mM and 25 mM) and no antioxidant (control). Diluted semen samples were kept in tubes and cooled from 37 to 5 °C in a cold cabinet, and maintained at 5 °C. Cooled samples were evaluated for sperm motility, viability, mitochondrial activity and acrosome integrity at 0, 24, 48, 72 and 96 h. Extender supplemented with trehalose (10 and 25 mM) and cysteine (2 and 4 mM) led to higher motility in comparison to the control at 24, 48, 72 and 96 h of liquid storage (P < 0.05). Trehalose at the doses of 10 mM, 25 mM and 2 mM cysteine led to higher viability between 24–48–72 h and at 96 h of liquid storage (P < 0.05). Further, 4 mM of cysteine improved sperm viability rates at 24 and 48 h of storage compared to the control group (P < 0.05), and resulted in improved acrosome integrity rates compared to the control group at 72 and 96 h of storage (P < 0.05). Extender supplemented with 10 and 25 mM trehalose at 24 and 72 h and 4 mM cysteine at 24 and 96 h of storage led to higher sperm mitochondrial activity rates when compared to the control group (P < 0.05). In conclusion, the findings of this study show that trehalose and cysteine provided significant protection to ram sperm parameters during liquid storage.

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The addition of docosahexaenoic acid (DHA) and antioxidants (glutathione peroxidase and superoxide dismutase) in extenders to epididymal sperm cryopreservation in bulls

Zygote ◽

10.1017/s0967199418000096 ◽

2018 ◽

Vol 26 (3) ◽

pp. 199-206 ◽

Cited By ~ 5

Author(s):

João D.A. Losano ◽

Daniel S.R. Angrimani ◽

Bruno R. Rui ◽

Luana C. Bicudo ◽

Andressa Dalmazzo ◽

...

Keyword(s):

Oxidative Stress ◽

Superoxide Dismutase ◽

Docosahexaenoic Acid ◽

Glutathione Peroxidase ◽

Genetic Material ◽

Sperm Viability ◽

Epididymal Sperm ◽

Sperm Cryopreservation ◽

Pufa Supplementation ◽

Dependent Effect

SummaryThe cryopreservation of epididymal sperm is an important technique that allows genetic material to be preserved, even post mortem. However, cryopreservation leads to increased oxidative stress and impaired sperm viability. Polyunsaturated fatty acid (PUFA) supplementation may improve certain sperm characteristics, but it also makes sperm more susceptible to oxidative stress, therefore adding antioxidants that counteract oxidative stress has become an option. In this context, this study aimed to evaluate the effect of the interaction between docosahexaenoic acid (DHA) and antioxidants on the quality after the cryopreservation of epididymal bull sperm. Twenty epididymides were collected after slaughter, and epididymal sperm was cryopreserved with bovine extender supplemented with docosahexaenoic acid (DHA), glutathione peroxidase (GPx) and superoxide dismutase (SOD). We verified an improvement in motility in the group that was treated only with DHA 5 µM and a concentration-dependent effect on susceptibility to lipid peroxidation that was associated with DHA concentration (1 µM, 5 µM or 10 µM). Moreover, treatment with DHA (5 µM) and SOD (20 IU/ml) resulted in higher sperm motility. Thus, the association between DHA (5 µM) and SOD (20 IU/ml) appears to be an option for increased epididymal sperm features in bulls.

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