scholarly journals The DAVID Gene Functional Classification Tool: a novel biological module-centric algorithm to functionally analyze large gene lists

2007 ◽  
Vol 8 (9) ◽  
pp. R183 ◽  
Author(s):  
Da Huang ◽  
Brad T Sherman ◽  
Qina Tan ◽  
Jack R Collins ◽  
W Gregory Alvord ◽  
...  
2018 ◽  
Vol 2018 ◽  
pp. 1-13 ◽  
Author(s):  
Zonggao Shi ◽  
Chunyan Li ◽  
Laura Tarwater ◽  
Jun Li ◽  
Yang Li ◽  
...  

We performed RNA-seq on an Illumina platform for 7 patients with endometrioid endometrial carcinoma for which both tumor tissue and adjacent noncancer tissue were available. A total of 66 genes were differentially expressed with significance level at adjusted p value < 0.01. Using the gene functional classification tool in the NIH DAVID bioinformatics resource, 5 genes were found to be the only enriched group out of that list of genes. The gene IGSF9 was chosen for further characterization with immunohistochemical staining of a larger cohort of human endometrioid carcinoma tissues. The expression level of IGSF9 in cancer cells was significantly higher than that in control glandular cells in paired tissue samples from the same patients (p=0.008) or in overall comparison between cancer and the control (p=0.003). IGSF9 expression is higher in patients with myometrium invasion relative to those without invasion (p=0.015). Reanalysis of RNA-seq dataset from The Cancer Genome Atlas shows higher expression of IGSF9 in endometrial cancer versus normal control and expression was associated with poor prognosis. These results suggest IGSF9 as a new biomarker in endometrial cancer and warrant further studies on its function, mechanism of action, and potential clinical utility.


Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2200
Author(s):  
Luis Fernando Méndez-López ◽  
Pierluigi Caboni ◽  
Eder Arredondo-Espinoza ◽  
Juan J. J. Carrizales-Castillo ◽  
Isaías Balderas-Rentería ◽  
...  

The bioassay-guided fractionation of a CHCl3-MeOH extract from the stems of Cissus trifoliata identified an active fraction against PC3 prostate cancer cells. The treatment for 24 h showed an 80% reduction in cell viability (p ≤ 0.05) by a WST-1 assay at a concentration of 100 μg/mL. The HPLC-QTOF-MS analysis of the fraction showed the presence of coumaric and isoferulic acids, apigenin, kaempferol, chrysoeriol, naringenin, ursolic and betulinic acids, hexadecadienoic and octadecadienoic fatty acids, and the stilbene resveratrol. The exposure of PC3 cells to resveratrol (IC25 = 23 μg/mL) for 24 h induced significant changes in 847 genes (Z-score ≥ ±2). The functional classification tool of the DAVID v6.8 platform indicates that the underlying molecular mechanisms against the proliferation of PC3 cells were associated (p ≤ 0.05) with the process of differentiation and metabolism. These findings provide experimental evidence suggesting the potential of C. trifoliata as a promising natural source of anticancer compounds.


2012 ◽  
Vol 43 (02) ◽  
Author(s):  
A Riess ◽  
U Grasshoff ◽  
U Hehr ◽  
M Bonin

2013 ◽  
Author(s):  
Carolina Moreno ◽  
Isabel Paiva ◽  
Leonor Gomes ◽  
Luisa Ruas ◽  
Sofia Gouveia ◽  
...  

Physiotherapy ◽  
2013 ◽  
Vol 21 (3) ◽  
Author(s):  
Natalia Uścinowicz ◽  
Wojciech Seidel ◽  
Paweł Zostawa ◽  
Sebastian Klich

AbstractThe recent Olympic Games in London incited much interest in the competition of disabled athletes. Various people connected with swimming, including coaches and athletes, have speculated about the fairness of competitions of disabled athletes. A constant problem are the subjective methods of classification in disabled sport. Originally, athletes with disabilities were classified according to medical diagnosis. Due to the injustice which still affects the competitors, functional classification was created shortly after. In the present review, the authors show the anomalies in the structure of the classification. The presented discovery led to the suggestion to introduce objective methods, thanks to which it would be no longer necessary to rely on the subjective assessment of the classifier. According to the authors, while using objective methods does not completely rule out the possibility of fraud by disabled athletes in the classification process, it would certainly reduce their incidence. Some of the objective methods useful for the classification of disabled athletes are: posturography, evaluation of the muscle parameters, electrogoniometric assessment, surface electromyography, and analysis of kinematic parameters. These methods have provide objective evaluation in the diagnostic sense but only if they are used in tandem. The authors demonstrate the undeniable benefits of using objective methods. Unfortunately, there are not only advantages of such solution, there also several drawbacks to be found. The conclusion of the article is the statement by the authors that it is right to use objective methods which allow to further the most important rule in sport: fair-play.


Sign in / Sign up

Export Citation Format

Share Document