scholarly journals In silico evidence of de novo interactions between ribosomal and Epstein - Barr virus proteins

2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Edmund Ui-Hang Sim ◽  
Shruti Prashant Talwar
Keyword(s):  
In Silico ◽  
Epstein Barr Virus ◽  
De Novo ◽  
Barr Virus ◽  
Epstein Barr ◽  
Virus Proteins

scholarly journals BGLF2 Increases Infectivity of Epstein-Barr Virus by Activating AP-1 uponDe NovoInfection

mSphere ◽  
2018 ◽  
Vol 3 (2) ◽  
Author(s):  
Natsuno Konishi ◽  
Yohei Narita ◽  
Fumiya Hijioka ◽  
H. M. Abdullah Al Masud ◽  
Yoshitaka Sato ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Epstein Barr Virus ◽  
De Novo ◽  
Physiological Role ◽  
Significant Loss ◽  
Viral Gene ◽  
Barr Virus ◽  
Cell Functions ◽  
Epstein Barr ◽  
Lytic Genes

ABSTRACTEpstein-Barr virus (EBV) is a human gammaherpesvirus that causes infectious mononucleosis and several malignancies, such as endemic Burkitt lymphoma and nasopharyngeal carcinoma. Herpesviruses carry genes that can modify cell functions, including transcription and ubiquitination, thereby facilitating viral growth and survival in infected cells. Using a reporter screening system, we revealed the involvement of several EBV gene products in such processes. Of these, BGLF2 activated the AP-1 signaling pathway through phosphorylation of p38 and c-Jun N-terminal kinase (JNK). Knockout of the BGLF2 gene did not affect viral gene expression and viral genome DNA replication, but resulted in marked reduction of progeny titer. We also found that the BGLF2 disruption resulted in significant loss of infectivity uponde novoinfection. Interestingly, expression of a binding partner, BKRF4, repressed the activation of AP-1 by BGLF2. These results shed light on the physiological role of the tegument protein BGLF2.IMPORTANCEEpstein-Barr virus (EBV), an oncogenic gammaherpesvirus, carries ~80 genes. While several genes have been investigated extensively, most lytic genes remain largely unexplored. Therefore, we cloned 71 EBV lytic genes into an expression vector and used reporter assays to screen for factors that activate signal transduction pathways, viral and cellular promoters. BGLF2 activated the AP-1 signaling pathway, likely by interacting with p38 and c-Jun N-terminal kinase (JNK), and increased infectivity of the virus. We also revealed that BKRF4 can negatively regulate AP-1 activity. Therefore, it is suggested that EBV exploits and modifies the AP-1 signaling pathway for its replication and survival.

scholarly journals Epstein-Barr Virus Proteins EBNA3A and EBNA3C Together Induce Expression of the Oncogenic MicroRNA Cluster miR-221/miR-222 and Ablate Expression of Its Target p57KIP2

2015 ◽  
Vol 11 (7) ◽  
pp. e1005031 ◽  
Author(s):  
Quentin Bazot ◽  
Kostas Paschos ◽  
Lenka Skalska ◽  
Jens S. Kalchschmidt ◽  
Gillian A. Parker ◽  
...  
Keyword(s):  
Epstein Barr Virus ◽  
Barr Virus ◽  
Microrna Cluster ◽  
Epstein Barr ◽  
Virus Proteins

scholarly journals Impact of donor and recipient Epstein-Barr Virus serostatus on outcomes of allogeneic hematopoietic cell transplantation: a systematic review and meta-analysis

2021 ◽  
Author(s):  
Michalina Kołodziejczak ◽  
◽  
Lidia Gil ◽  
Rafael de la Camara ◽  
Jan Styczyński
Keyword(s):  
Clinical Outcomes ◽  
Graft Versus Host Disease ◽  
Epstein Barr Virus ◽  
De Novo ◽  
Meta Analysis ◽  
Graft Versus Host ◽  
Hematological Diseases ◽  
Barr Virus ◽  
Epstein Barr ◽  
Meta Analyses

AbstractAllogeneic hematopoietic cell transplant (allo-HCT) is a potentially curative therapeutic strategy that showed encouraging long-term outcomes in hematological diseases. A number of factors can influence post-transplant clinical outcomes. While Epstein-Barr virus (EBV) constitutes a trigger for development of various adverse conditions, no clinical study yet has been powered to assess the effect of EBV serostatus on the clinical outcomes in allo-HCT population. To systematically summarize and analyze the impact of donor and recipient EBV serostatus on transplant outcomes in allo-HCT recipients, meta-analyses were conducted. Selected endpoints were overall survival (OS), relapse-free survival (RFS), relapse incidence (RI), non-relapse mortality (NRM), acute graft-versus-host disease (aGVHD), chronic graft-versus-host disease (cGVHD), and de novo cGVHD. Three studies with 26,650 patients, transplanted for acute leukemias, lymphomas, chronic hematological malignancies, or non-malignant hematological diseases were included in the meta-analysis. In the whole population, with a total of 53,300 donors and recipients, the rate of EBV seropositivity was 85.1%, including 86.6% and 83.6% among transplant recipients and healthy donors, respectively. Donor EBV seropositivity increased the risk of cGVHD by 17%, de novo cGVHD by 14%, and aGHVD by 5%. Recipient EBV seropositivity increased the risk of cGVHD by 12%, de novo cGVHD by 17%; increased NRM by 11%, increased RI by 11%, decreased OS by 14%, and decreased RFS by 11%. In performed meta-analyses, donor and recipient EBV seropositivity was found to have a significant impact on transplant outcomes in patients after allo-HCT.

Role of Cytomegalovirus and Epstein-Barr virus in Patients with de Novo Colon Cancer after Renal Transplantation

2006 ◽  
Vol 92 (3) ◽  
pp. 219-221 ◽  
Author(s):  
Gian Luigi Adani ◽  
Umberto Baccarani ◽  
Dario Lorenzin ◽  
Vittorio Bresadola ◽  
Giuseppe Currò ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Renal Transplantation ◽  
Epstein Barr Virus ◽  
De Novo ◽  
Barr Virus ◽  
Epstein Barr

scholarly journals Epstein-Barr virus modulates de novo protein synthesis in human neutrophils

Blood ◽  
1995 ◽  
Vol 86 (7) ◽  
pp. 2789-2798 ◽  
Author(s):  
AD Beaulieu ◽  
R Paquin ◽  
J Gosselin
Keyword(s):  
Protein Synthesis ◽  
Host Response ◽  
Epstein Barr Virus ◽  
Rna Synthesis ◽  
De Novo ◽  
Interleukin 1 ◽  
Human Neutrophils ◽  
Two Dimensional Gel Electrophoresis ◽  
Barr Virus ◽  
Epstein Barr

Neutrophils and macrophages represent the first line of defense against microbial invaders. However, the role of phagocytes in host response to viral infection is poorly understood. We have previously shown that Epstein-Barr virus (EBV) interacts with human monocytes and modulates cytokine production in this cell type, but its effects on neutrophils are still unknown. In the present study, we investigated the presence of EBV receptor (CR2 or CD21) on neutrophils by cytofluorometry using five different anti-CD21 monoclonal antibodies (MoAbs), as well as fluoroscein isothiocyanate-EBV (FITC-EBV). Whereas no significant amount of neutrophils reacted with anti-CD21 MoAbs, studies with FITC-EBV indicated that viral particles bind to 30% of cells (in some individuals, EBV binds to more than 50% of neutrophils). This interaction is specific as it was completely inhibited by nonconjugated virus or with labeled virus preincubated with neutralizing MoAbs. After EBV treatment, cellular aggregation was observed in neutrophil cultures, an indication that neutrophils were activated. Although EBV did not induce respiratory burst activity in neutrophils, pretreatment with infectious particles enhanced (priming effect) the fMLP-induced O2-release in neutrophils. Instead of restricting our analysis to specific cytokine genes, we investigated the effects of EBV on neutrophil transcriptional events in general. The effect of this virus on de novo synthesis of total cellular RNA was first investigated by measuring the incorporation of [5–3H] uridine into total RNA. The results showed that RNA synthesis in neutrophils was significantly increased (2.3- to 21.3-fold) by EBV compared with the unstimulated controls. Live and UV-inactivated virus markedly induced RNA synthesis, whereas heat-inactivated virus lost this ability. Induction of RNA transcription was EBV specific, as an EBV-neutralizing antiserum abolished this effect. Induction of protein synthesis was also studied by measuring the incorporation of [35S] methionine and [35S] cysteine into secreted and intracellular proteins in neutrophils incubated with EBV. The synthesis of both secreted and cytoplasmic proteins was induced by EBV. One- and two-dimensional gel electrophoresis analysis showed that EBV modulates protein synthesis, because activation of the synthesis of certain proteins was accompanied by the inhibition of others. Interleukin-1 beta (IL-1 beta) and IL-1 receptor antagonist (IL-1Ra) synthesis was found to be induced by EBV. Therefore, modulation of host-response proteins such as IL-1Ra could be one of the many mechanisms by which this virus avoids rejection.(ABSTRACT TRUNCATED AT 400 WORDS)

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