scholarly journals Puccinia triticina pathotypes THTT and THTS display complex transcript profiles on wheat cultivar Thatcher

BMC Genetics ◽  
2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Jie Wei ◽  
Liping Cui ◽  
Na Zhang ◽  
Dongdong Du ◽  
Qingfang Meng ◽  
...  
Keyword(s):  
Wheat Cultivar ◽  
Puccinia Triticina ◽  
Transcript Profiles

scholarly journals Puccinia triticina pathotypes THTT and THTS display complex transcript profiles on wheat cultivar Thatcher

2020 ◽  
Author(s):  
jie Wei ◽  
Liping Cui ◽  
na Zhang ◽  
dongdong Du ◽  
qingfang Meng ◽  
...  
Keyword(s):  
Transcription Initiation ◽  
Wheat Cultivar ◽  
Early Stage ◽  
Puccinia Triticina ◽  
Differentially Expressed ◽  
Post Inoculation ◽  
Rna Seq ◽  
Initiation Factors ◽  
Transcript Profiles ◽  
Important Disease

Abstract Background: Wheat leaf rust is an important disease worldwide. Understanding the pathogenic molecular mechanism of Puccinia triticina Eriks . ( Pt ) and the inconstant toxic region is critical for managing the disease. The present study aimed to analyze the pathogenic divergence between Pt isolates. Results: Total RNA was extracted from the wheat cultivar Thatcher infected by two Pt isolates, Tc361_1 (THTT) and Tc284_2 (THTS), at 144 hours post inoculation (hpi). The mRNA was then sequenced, and a total of 2,784 differentially expressed genes (DEGs) were detected. Forty-five genes were specifically expressed in THTT; these genes included transcription initiation factors and genes with transmembrane transporter activity and other genes. Twenty-six genes were specifically expressed in THTS, including genes with GTPase activity, ABC transporters and other genes. Fifty-four differentially expressed candidate effectors were screened from the two isolates. Two candidate effectors were chosen and validated on tobacco, and the results showed that they could inhibit necrosis induced by BAX. qRT-PCR of 12 significant DEGs was carried out to validate that the results are similar to those of RNA-seq at 144 hpi, to show the expression levels of these DEGs in the early stage and to elucidate the differences in expression between the two Pt pathotypes. Conclusion: The results obtained in this study showed that although the two pathotypes of THTT and THTS contribute similar virulence to wheat, there are a large number of genes participate in the interaction with the susceptible wheat cultivar Thatcher, and revealed the pathogenicity of rust is very complicated.

scholarly journals Puccinia triticina pathotypes THTT and THTS display complex transcript profiles on wheat cultivar Thatcher

2020 ◽  
Author(s):  
jie Wei ◽  
Liping Cui ◽  
na Zhang ◽  
dongdong Du ◽  
qingfang Meng ◽  
...  
Keyword(s):  
Transcription Initiation ◽  
Wheat Cultivar ◽  
Early Stage ◽  
Puccinia Triticina ◽  
Differentially Expressed ◽  
Post Inoculation ◽  
Rna Seq ◽  
Initiation Factors ◽  
Transcript Profiles ◽  
Important Disease

Abstract Background: Wheat leaf rust is an important disease worldwide. Understanding the pathogenic molecular mechanism of Puccinia triticina Eriks. (Pt) and the inconstant toxic region is critical for managing the disease. The present study aimed to analyze the pathogenic divergence between Pt isolates. Results: Total RNA was extracted from the wheat cultivar Thatcher infected by two Pt isolates, Tc361_1 (THTT) and Tc284_2 (THTS), at 144 hours post inoculation (hpi). The mRNA was then sequenced, and a total of 2,784 differentially expressed genes (DEGs) were detected. Forty-five genes were specifically expressed in THTT; these genes included transcription initiation factors and genes with transmembrane transporter activity and other genes. Twenty-six genes were specifically expressed in THTS, including genes with GTPase activity, ABC transporters and other genes. Fifty-four differentially expressed candidate effectors were screened from the two isolates. Two candidate effectors were chosen and validated on tobacco, and the results showed that they could inhibit necrosis induced by BAX. qRT-PCR of 12 significant DEGs was carried out to validate that the results are similar to those of RNA-seq at 144 hpi, to show the expression levels of these DEGs in the early stage and to elucidate the differences in expression between the two Pt pathotypes. Conclusion: The results obtained in this study showed that although the two pathotypes of THTT and THTS contribute similar virulence to wheat, there are a large number of genes participate in the interaction with the susceptible wheat cultivar Thatcher, and revealed the pathogenicity of rust is very complicated.

scholarly journals Puccinia triticina pathotypes- THTT and THTS display complex transcript profiles on wheat cultiver Thatcher

2020 ◽  
Author(s):  
jie Wei ◽  
Liping Cui ◽  
dongdong Du ◽  
na Zhang ◽  
qingfang Meng ◽  
...  
Keyword(s):  
Transcription Initiation ◽  
Early Stage ◽  
Puccinia Triticina ◽  
Differentially Expressed ◽  
Post Inoculation ◽  
Rna Seq ◽  
Initiation Factors ◽  
Transcript Profiles ◽  
Abc Transporter Genes ◽  
Important Disease

Abstract Background: Wheat leaf rust is an important disease worldwide. Understanding the Puccinia triticina f. sp. tritici (Pt) pathogenic molecular mechanism and the inconstant toxic region is critical for managing the disease. The present study aimed to analyze pathogenic divergence between Pt isolates.Results: Total RNA was extracted from wheat cultiver Thatcher infected by two Pt isolates, Tc361_1 (THTT) and Tc284_2 (THTS) at 144 hours post inoculation (hpi). The mRNA was then sequenced, and a total of 2,784 differentially expressed genes (DEGs) were detected. Forty-five genes were specifically expressed in THTT; these genes included transcription initiation factors and genes with transmembrane transporter activity and other genes. Twenty-six genes were specifically expressed in THTS, including genes with GTPase activity, ABC transporter genes and other genes. Fifty-four differentially expressed candidate effectors were screened from the two isolates. Two candidate effectors were chosen and validated on tobacco, and the results showed that they could inhibit necrosis induced by Bax. qRT-PCR of 12 significant DEGs was carried out to validate the results are similar to that of RNA-seq at 144 hpi, to show the expression levels of these DEGs in the early stage and to elucidate the differences in expression between the two Pt pathotypes.Conclusion: The results obtained in this study showed that the two pathtypes of THTT and THTS although contribute similar virulence to wheat, there are a large number of genes participate in the interaction with susceptible wheat cultivar Thatcher, and revealed the pathgenicity of rust is very complicated.

scholarly journals A Combination of Leaf Rust Resistance Genes, Including Lr34 and Lr46, Is the Key to the Durable Resistance of the Canadian Wheat Cultivar, Carberry

2022 ◽  
Vol 12 ◽  
Author(s):  
Firdissa E. Bokore ◽  
Ron E. Knox ◽  
Colin W. Hiebert ◽  
Richard D. Cuthbert ◽  
Ron M. DePauw ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Wheat Cultivar ◽  
Durable Resistance ◽  
Snp Array ◽  
Puccinia Triticina ◽  
Leaf Rust Resistance ◽  
Adult Plant ◽  
Dh Population

The hexaploid spring wheat cultivar, Carberry, was registered in Canada in 2009, and has since been grown over an extensive area on the Canadian Prairies. Carberry has maintained a very high level of leaf rust (Puccinia triticina Eriks.) resistance since its release. To understand the genetic basis of Carberry’s leaf rust resistance, Carberry was crossed with the susceptible cultivar, Thatcher, and a doubled haploid (DH) population of 297 lines was generated. The DH population was evaluated for leaf rust in seven field environments at the adult plant stage. Seedling and adult plant resistance (APR) to multiple virulence phenotypes of P. triticina was evaluated on the parents and the progeny population in controlled greenhouse studies. The population was genotyped with the wheat 90 K iSelect single nucleotide polymorphism (SNP) array, and quantitative trait loci (QTL) analysis was performed. The analysis using field leaf rust response indicated that Carberry contributed nine QTL located on chromosomes 1B, 2B (2 loci), 2D, 4A, 4B, 5A, 5B, and 7D. The QTL located on 1B, 2B, 5B, and 7D chromosomes were observed in two or more environments, whereas the remainder were detected in single environments. The resistance on 1B, detected in five environments, was attributed to Lr46 and on 7D, detected in seven environments to Lr34. The first 2B QTL corresponded with the adult plant gene, Lr13, while the second QTL corresponded with Lr16. The seedling analysis showed that Carberry carries Lr2a, Lr16, and Lr23. Five epistatic effects were identified in the population, with synergistic interactions being observed for Lr34 with Lr46, Lr16, and Lr2a. The durable rust resistance of Carberry is attributed to Lr34 and Lr46 in combination with these other resistance genes, because the resistance has remained effective even though the P. triticina population has evolved virulent to Lr2a, Lr13, Lr16, and Lr23.

scholarly journals Puccinia triticina pathotypes-THTT and THTS display complex transcript profiles on Thatcher

2019 ◽  
Author(s):  
jie Wei ◽  
dongdong Du ◽  
na Zhang ◽  
qingfang Meng ◽  
hongfei Yan ◽  
...  
Keyword(s):  
Transcription Initiation ◽  
Early Stage ◽  
Puccinia Triticina ◽  
Initiation Factor ◽  
Post Inoculation ◽  
Qrt Pcr ◽  
Transcription Initiation Factor ◽  
Solid Foundation ◽  
The Difference ◽  
Transcript Profiles

Abstract Background: Wheat leaf rust is an important disease worldwide. Understanding the Puccinia triticina (Pt) pathogenic molecular mechanism and inconstant toxic region are highly important for managing the disease. The present study aimed to analyze pathogenic divergences between Pt isolates. Results: Total RNA was extracted from Thatcher infected by two Pt isolates, Tc361_1 (THTT) and Tc284_2 (THTS) at 144 hours post inoculation (hpi). The mRNA then was harvested and sequenced. Results indicated that a total of 2,784 differential expressed genes (DEGs) were detected. Forty-five of genes were specifically expressed in Tc361_1 including transcription initiation factor, transmembrane transporter activity and so on, while 26 of genes were specially expressed in Tc284_2 including GTPase activity, ABC transporter and so on. Sixty-seven differential expressed candidate effectors were screened. qRT-PCR of 14 significantly different expressed genes were carried out to prove that the results of RNA-seq is similar to that of qRT-PCR at 144 hpi and show their expression level in the early stage and the difference between two Pt pathotypes, which revealed the complex transcript profiles. Conclusion: The results obtained in this study might have a solid foundation for the future studies on clarifying the mechanism of pathogenicity differences of Chinese isolates and pathogenic mechanism of Pt.

scholarly journals Puccinia triticina pathotypes-THTT and THTS display complex transcript profiles on Thatcher

2020 ◽  
Author(s):  
jie Wei ◽  
Liping Cui ◽  
dongdong Du ◽  
na Zhang ◽  
qingfang Meng ◽  
...  
Keyword(s):  
Transcription Initiation ◽  
Early Stage ◽  
Puccinia Triticina ◽  
Initiation Factor ◽  
Post Inoculation ◽  
Qrt Pcr ◽  
Transcription Initiation Factor ◽  
Differential Expressed Genes ◽  
The Difference ◽  
Transcript Profiles

Abstract Background: Wheat leaf rust is an important disease worldwide. Understanding the Puccinia triticina f.sp tritici (Pt) pathogenic molecular mechanism and inconstant toxic region are highly important for managing the disease. The present study aimed to analyze pathogenic divergences between Pt isolates. Results: Total RNA was extracted from Thatcher infected by two Pt isolates, Tc361_1 (THTT) and Tc284_2 (THTS) at 144 hours post inoculation (hpi). The mRNA then was harvested and sequenced. Results indicated that a total of 2,784 differential expressed genes (DEGs) were detected. Forty-five genes were specifically expressed in THTT including transcription initiation factor, transmembrane transporter activity and so on, while 26 genes were specially expressed in THTS including GTPase activity, ABC transporter and so on. Fifty-four differential expressed candidate effectors were screened from the tow isolates. Two candidate effectors were chosen and validated on tabacco, and the result showed that they can inhibit the necrosis induced by Bax. qRT-PCR of 12 significantly different expressed genes were carried out to prove that the results of RNA-seq is similar to that of qRT-PCR at 144 hpi and show their expression level in the early stage and the difference between two Pt pathotypes.Conclusion: The results obtained in this study showed that the transcript profiles between THTT or THTS and Thatcher were very complicated.

scholarly journals Physiologic specialization of wheat leaf rust (Puccinia triticina Eriks.) in the Slovak Republic in 2009–2011

2012 ◽  
Vol 48 (No. 3) ◽  
pp. 101-107 ◽  
Author(s):  
A. Hanzalová ◽  
T. Sumíková ◽  
J. Huszár ◽  
P. Bartoš
Keyword(s):  
Winter Wheat ◽  
Leaf Rust ◽  
Slovak Republic ◽  
Wheat Cultivar ◽  
Puccinia Triticina ◽  
Wheat Leaf Rust ◽  
Winter Wheat Cultivar ◽  
Wheat Leaf ◽  
Winter Wheat Cultivars ◽  
Almost All

In 2009–2011 virulence of the wheat leaf rust population was studied on Thatcher near-isogenic lines with Lr1, Lr2a, Lr2b, Lr2c, Lr3a, Lr9, Lr11, Lr13, Lr15, Lr17, Lr19, Lr21, Lr23, Lr24, Lr26 and Lr28. Samples of leaf rust were obtained from different parts of the Slovak Republic. A total of 122 wheat leaf rust isolates were analysed. Resistance gene Lr19 was effective to all tested isolates. Virulence to Lr9 was found, however only in one isolate. Gene Lr24 conditioned resistance to almost all rust collections. A lower frequency of virulence to Lr2a and Lr28 was also observed. Nineteen winter wheat cultivars grown in Slovakia were tested with 8 leaf rust isolates. Winter wheat cultivar Bona Dea was resistant to all isolates applied in the greenhouse test. Presence of Lr genes was estimated according to the reactions of the tested cultivars. Presence of Lr10, Lr26, Lr34 and Lr37 was studied by molecular markers.

Variation of Catechin, epicatechin and their enantiomers concentrations before and after wheat cultivar-Puccinia triticina infection

2011 ◽  
Vol 125 (4) ◽  
pp. 1287-1290 ◽  
Author(s):  
Alireza Ghassempour ◽  
Saeed Mollayi ◽  
Mohsen Farzaneh ◽  
Abbas Sharifi-Tehrani ◽  
Hassan Y. Aboul-Enein
Keyword(s):  
Wheat Cultivar ◽  
Puccinia Triticina ◽  
Before And After

scholarly journals Non-hypersensitive leaf rust resistance of bread wheat cultivar PBW65 conditioned by genes different fromLr34

2009 ◽  
Vol 45 (No. 1) ◽  
pp. 26-30 ◽  
Author(s):  
M.A. Khan ◽  
R.G. Saini
Keyword(s):  
Leaf Rust ◽  
Bread Wheat ◽  
Rust Resistance ◽  
Wheat Cultivar ◽  
Infection Type ◽  
Puccinia Triticina ◽  
Leaf Rust Resistance ◽  
Triticum Aestivum L ◽  
Resistance Level ◽  
Highly Virulent

: The bread wheat (Triticum aestivum L.) cultivar PBW65 has shown hight levels of resistance to the most frequent and highly virulent Indian race 77-5 of leaf rust (Puccinia triticina). The infection type and disease severity indicated a non-hypersensitive type of resistance against the race 77-5 in PBW65. The cultivar PBW65 was crossed with the leaf rust susceptible cultivar WL711 to determine the mode of inheritance of the resistance. The segregation for resistant and susceptible plants in the F<sub>2</sub> and F<sub>3</sub> generations revealed, that two genes, each showing additive effects, were likely to confer resistance to leaf rust in PBW65. Intercrossing of PBW65 with Cook (Lr34), RL6058 (Lr34) and HD2009, possessing a similar resistance level like PBW65, revealed that the genes for leaf rust resistance in PBW65 were non-allelic to Cook (Lr34), RL6058 (Lr34) as well as to the gene(s) in HD2009. It is concluded that the cultivar PBW65 is a novel source of non-hypersensitive leaf rust resistance.

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