scholarly journals Identification and characterization of long noncoding RNAs and mRNAs expression profiles related to postnatal liver maturation of breeder roosters using Ribo-zero RNA sequencing

BMC Genomics ◽  
2018 ◽  
Vol 19 (1) ◽  
Author(s):  
Shengru Wu ◽  
Yanli Liu ◽  
Wei Guo ◽  
Xi Cheng ◽  
Xiaochun Ren ◽  
...  
Keyword(s):  
Rna Sequencing ◽  
Expression Profiles ◽  
Noncoding Rnas ◽  
Long Noncoding Rnas ◽  
Identification And Characterization

RNA sequencing identifies two novel liver-specific long noncoding RNAs with potential diagnostic value in hepatocellular carcinoma

2021 ◽  
Author(s):  
Chao Tan ◽  
Xi Zeng ◽  
Meile Mo ◽  
Xiaoyun Ma ◽  
Qiuli Liang ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Rna Sequencing ◽  
Reverse Transcription ◽  
Expression Profiles ◽  
Noncoding Rnas ◽  
Area Under The Curve ◽  
Long Noncoding Rnas ◽  
Normal Tissues ◽  
Quantitative Reverse Transcription Pcr ◽  
Reverse Transcription Pcr

Aim: To explore the expression profiles of long noncoding RNAs (lncRNAs) and identify novel lncRNAs as biomarkers for early diagnosis and therapy of hepatocellular carcinoma (HCC). Materials & methods: Expression profiles of lncRNAs and mRNAs in five paired HCC and adjacent normal tissues were obtained by RNA sequencing. Eight lncRNAs, including two novel liver-specific lncRNAs (NONHSAT059247.2 and NONHSAT013897.2), were validated in another 74 pairs of HCC and adjacent normal tissues by quantitative reverse transcription PCR. Results: The results of quantitative reverse transcription PCR showed that NONHSAT252133.1, NONHSAT112116.2 and NONHSAT242657.1 were significantly upregulated in HCC tissues, whereas NONHSAT169790.1, NONHSAT059247.2 and NONHSAT013897.2 were significantly downregulated. Two liver-specific lncRNAs demonstrated excellent diagnostic performance: NONHSAT059247.2 (area under the curve = 0.941, 95% CI: 0.902–0.979, p < 0.0001), NONHSAT013897.2 (area under the curve = 0.944, 95% CI: 0.906–0.983, p < 0.0001). Conclusion: The liver-specific lncRNAs NONHSAT059247.2 and NONHSAT013897.2, may provide new biomarkers for the future study on diagnosis, therapy, and mechanisms of HCC.

scholarly journals Identification and Characterization of Long Noncoding RNAs in Ovine Skeletal Muscle

Animals ◽  
2018 ◽  
Vol 8 (7) ◽  
pp. 127
Author(s):  
Qing Li ◽  
Ruizao Liu ◽  
Huijing Zhao ◽  
Ran Di ◽  
Zengkui Lu ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Gastrocnemius Muscle ◽  
Muscle Development ◽  
Noncoding Rnas ◽  
Long Noncoding Rnas ◽  
Differentially Expressed ◽  
Pairwise Comparisons ◽  
Cellular Processes ◽  
Identification And Characterization

Long noncoding RNAs (lncRNAs) are increasingly being recognized as key regulators in many cellular processes. However, few reports of them in livestock have been published. Here, we describe the identification and characterization of lncRNAs in ovine skeletal muscle. Eight libraries were constructed from the gastrocnemius muscle of fetal (days 85 and 120), newborn and adult Texel and Ujumqin sheep. The 2002 identified transcripts shared some characteristics, such as their number of exons, length and distribution. We also identified some coding genes near these lncRNA transcripts, which are particularly associated with transcriptional regulation- and development-related processes, suggesting that the lncRNAs are associated with muscle development. In addition, in pairwise comparisons between the libraries of the same stage in different breeds, a total of 967 transcripts were differentially expressed but just 15 differentially expressed lncRNAs were common to all stages. Among them, we found that TCONS_00013201 exhibited higher expression in Ujumqin samples, while TCONS_00006187 and TCONS_00083104 were higher in Texel samples. Moreover, TCONS_00044801, TCONS_00008482 and TCONS_00102859 were almost completely absent from Ujumqin samples. Our results suggest that differences in the expression of these lncRNAs may be associated with the muscular differences observed between Texel and Ujumqin sheep breeds.

scholarly journals Analysis of expression profiles of long noncoding RNAs and mRNAs in brains of mice infected by rabies virus by RNA sequencing

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Pingsen Zhao ◽  
Sudong Liu ◽  
Zhixiong Zhong ◽  
Tianqi Jiang ◽  
Ruiqiang Weng ◽  
...  
Keyword(s):  
Rna Sequencing ◽  
Rabies Virus ◽  
Expression Profiles ◽  
Noncoding Rnas ◽  
Long Noncoding Rnas

scholarly journals Identification and characterization of functional long noncoding RNAs in cancer

2020 ◽  
Vol 34 (12) ◽  
pp. 15630-15646
Author(s):  
Christiane E. Olivero ◽  
Nadya Dimitrova
Keyword(s):  
Noncoding Rnas ◽  
Long Noncoding Rnas ◽  
Identification And Characterization

scholarly journals Identification and characterization of long noncoding RNAs and their association with acquisition of blood meal in Culex quinquefasciatus

2020 ◽  
Author(s):  
Azali Azlan ◽  
Mardani Abdul Halim ◽  
Faisal Mohamad ◽  
Ghows Azzam
Keyword(s):  
Culex Quinquefasciatus ◽  
Blood Meal ◽  
Noncoding Rnas ◽  
Gc Content ◽  
Blood Feeding ◽  
Long Noncoding Rnas ◽  
Host Interaction ◽  
Systematic Identification ◽  
Identification And Characterization

AbstractThe Southern house mosquito, Culex quinquefasciatus (Cx. quinquefasciatus) is an important vector that transmit multiple diseases including West Nile encephalitis, Japanese encephalitis, St. Louis encephalitis and lymphatic filariasis. Long noncoding RNAs (lncRNAs) involve in many biological processes such development, infection, and virus-host interaction. However, there is no systematic identification and characterization of lncRNAs in Cx. quinquefasciatus. Here, we report the first ever lncRNA identification in Cx. quinquefasciatus. By using 31 public RNA-seq datasets, a total of 4,763 novel lncRNA transcripts were identified, of which 3,591, 569, and 603 were intergenic, intronic, and antisense respectively. Examination of genomic features revealed that Cx. quinquefasciatus shared similar characteristics with other species such as short in length, low GC content, low sequence conservation, and low coding potential. Furthermore, compared to protein-coding genes, Cx. quinquefasciatus lncRNAs had lower expression values, and tended to be expressed in temporally-specific fashion. In addition, weighted correlation network and functional annotation analyses showed that lncRNAs may have roles in blood meal acquisition of adult female Cx. quinquefasciatus mosquitoes. This study presents the first systematic identification and analysis of Cx. quinquefasciatus lncRNAs and their association with blood feeding. Results generated from this study will facilitate future investigation on the function of Cx. quinquefasciatus lncRNAs.

Identification and characterization of proliferative retinopathy-related long noncoding RNAs

2015 ◽  
Vol 465 (3) ◽  
pp. 324-330 ◽  
Author(s):  
Rong-Mei Zhou ◽  
Xiao-Qun Wang ◽  
Jin Yao ◽  
Yi Shen ◽  
Sai-Nan Chen ◽  
...  
Keyword(s):  
Noncoding Rnas ◽  
Proliferative Retinopathy ◽  
Long Noncoding Rnas ◽  
Identification And Characterization
Sign in / Sign up

Export Citation Format

Share Document