scholarly journals Silver diamine fluoride (SDF) used in childhood caries management has potent antifungal activity against oral Candida species

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Kausar Sadia Fakhruddin ◽  
Hiroshi Egusa ◽  
Hien Chi Ngo ◽  
Chamila Panduwawala ◽  
Siripen Pesee ◽  
...  
2020 ◽  
Author(s):  
Kausar Sadia Fakhruddin ◽  
Hiroshi Egusa ◽  
Hien Chi Ngo ◽  
Chamila Panduwawala ◽  
Siripen Pesee ◽  
...  

Abstract Background: The microbiome of Severe-Early Childhood Caries (S-ECC), is characterized by an ecosystem comprising bacterial and fungal species, with a predominance of Candida species. Hence, an anti-cariogen effective against both bacteria and fungi would be valuable in the management of S-ECC. Here we evaluate the antifungal effect of silver diamine fluoride (SDF) against 35-clinical yeast isolates (Ten-each of C. albicans , C. krusei, C. tropicalis and five C. glabrata strains) from dentinal caries-lesions from S-ECC. Results: Disc-diffusion and time-kill assays as well as MIC 50 and MIC 90 evaluations against therapeutic concentrations confirmed the broad-spectrum anti-candidal potency of SDF. Ultrastructural images revealed morphologic aberrations of yeast-cell walls on exposure to SDF. All C. krusei and C. glabrata isolates were significantly more sensitive to SDF, relative to the standard antifungal fluconazole. Further, SDF appears to effectively abrogate filamentation of C. albicans even at very low concentrations. Conclusions: Our data, for the first time, elucidate the anti-candidal potency of SDF, in addition to its known antibacterial activity, in the management of S-ECC.


2020 ◽  
Author(s):  
Kausar Sadia Fakhruddin ◽  
Lakshman Samaranayake ◽  
Hiroshi Egusa ◽  
Hien Chi Ngo ◽  
Venkatachalam Thenmozhi ◽  
...  

Abstract Background: The microbiome of Severe-Early Childhood Caries (S-ECC), is characterized by an ecosystem comprising bacterial and fungal species, with a predominance of Candida species. Hence, an anti-cariogen effective against both bacteria and fungi would be valuable in the management of S-ECC. Methods : Here we evaluate the antifungal effect of silver diamine fluoride (SDF) against 35-clinical yeast isolates (Ten-each of C. albicans , C. krusei, C. tropicalis and five C. glabrata strains) from dentinal caries-lesions from S-ECC. Results: Disc-diffusion and time-kill assays as well as MIC 50 and MIC 90 evaluations against therapeutic concentrations confirmed the broad-spectrum anti-candidal potency of SDF. Ultrastructural images revealed morphologic aberrations of yeast-cell walls on exposure to SDF. All C. krusei and C. glabrata isolates were significantly more sensitive to SDF, relative to the standard antifungal fluconazole. Further, SDF appears to effectively abrogate filamentation of C . albicans even at very low concentrations. Conclusions: Our data, for the first time, elucidate the antifungal potency of SDF, in addition to its known antibacterial activity, in the management of S-ECC.


2019 ◽  
Author(s):  
Kausar Sadia Fakhruddin ◽  
Lakshman Samaranayake ◽  
Hiroshi Egusa ◽  
Hien Chi Ngo ◽  
Venkatachalam Thenmozhi ◽  
...  

Abstract Background The microbiome of Severe-Early Childhood Caries (S-ECC), is characterized by an ecosystem comprising bacterial and fungal species, with a predominance of Candida species. Hence, an anti-cariogen effective against both bacteria and fungi would be valuable in the management of S-ECC.Methods Here we evaluate the antifungal effect of silver diamine fluoride (SDF) against 35-clinical yeast isolates (Ten-each of C. albicans , C. krusei, C. tropicalis and five C. glabrata strains) from dentinal caries-lesions from S-ECC.Results Disc-diffusion and time-kill assays as well as MIC 50 and MIC 90 evaluations against therapeutic concentrations confirmed the broad-spectrum anti-candidal potency of SDF. Ultrastructural images revealed morphologic aberrations of yeast-cell walls on exposure to SDF. All C. krusei and C. glabrata isolates were significantly more sensitive to SDF, relative to the standard antifungal fluconazole.Conclusions Our data, for the first time, elucidate the antifungal potency of SDF, in addition to its known antibacterial activity, in the management of S-ECC.


2015 ◽  
Vol 9 (2) ◽  
pp. 42-47 ◽  
Author(s):  
Becker Onofre Sideney ◽  
Quinteiro dos Santos Zipora Morgana ◽  
Yumi Kagimura Francini ◽  
Paula Mattiello Shaiana

SpringerPlus ◽  
2016 ◽  
Vol 5 (1) ◽  
Author(s):  
Wan Harun Himratul-Aznita ◽  
Che Omran Nor-Zulaila ◽  
Khairuddin Nurul-Fatihah

2020 ◽  
pp. 238008442093069
Author(s):  
G. Kyoon-Achan ◽  
R.J. Schroth ◽  
H. Martin ◽  
M. Bertone ◽  
B.A. Mittermuller ◽  
...  

Background: The use of silver diamine fluoride (SDF) as a nonsurgical caries management product is growing. Evidence suggests that SDF is very successful in arresting caries. However, a common concern with SDF treatment is the unaesthetic black staining. The purpose of this qualitative study was to determine parents’ views following their children’s treatment with SDF to manage severe early childhood caries (ECC). Method: Parents were interviewed as part of a mixed-method study of SDF to arrest severe ECC. Children with caries lesions in primary teeth were treated with 2 applications of 38% SDF, followed by fluoride varnish. Semistructured in-person and phone interviews were conducted with 19 parents of children in the study. Data were transcribed verbatim and manually coded and uploaded to NVivo 12 for further coding analysis. Results: None of the parents had previously heard about SDF, and they learned about it from the study dentist. Although parents trusted the dentist’s information on SDF, they welcomed additional evidence, especially relating to product safety and effectiveness. Some parents were minimally concerned with the black staining caused by SDF treatment. It was more important that SDF arrested caries progression, minimized pain and sensitivity, and prevented dental infection. However, some parents expressed concerns related to the unaesthetic black staining. Interestingly, many parents indicated that their children were not overly concerned with the black staining. A majority of parents said that they would recommend the treatment to others. Conclusion: This is the first qualitative study involving parents of children who were treated with SDF. Most parents were accepting of SDF as a nonsurgical treatment to arrest caries and minimize dentinal sensitivity secondary to caries, although some expressed concern about the black staining in anterior teeth. It is important to adequately inform parents of the negative aesthetic consequences and obtain informed consent before treatment. Knowledge Transfer Statement: This qualitative study revealed that many parents of children with severe ECC are accepting of SDF as a nonrestorative caries management option, despite the black staining of caries lesions. Dental professionals need to be aware of these parental concerns and obtain written informed consent prior to treatment. Parents also requested more information and resources on SDF on its benefits, effectiveness, and any associated risks.


Author(s):  
Sayali Deshpande ◽  
Amit Reche ◽  
Priyanka Paul Madhu ◽  
Kumar Gaurav Chhabra ◽  
Sunaina Mutyalwar

Various studies published in the literature around the world indicate a significant prevalence of early childhood caries (ECC). Treatment of early childhood caries (ECC) using silver diamine fluoride (SDF) has become a viable alternative, particularly for young and anxious children, because traditional restorative treatments is unlikely to combat this prevalent condition. Arrested caries is identified by the hardening of teeth and it changes its color from dark brown to black. More restorative treatments can be performed if necessary. Traditional ECC restorative treatments are not always inexpensive or feasible, as they involve patient cooperation to achieve a satisfactory result. Japan was the first country to promote silver diamine fluoride in 1960. SDF has been used to prevent the spread of caries in various Asian nations, including Japan and China. It was enacted as a fluoride to treat sensitive teeth in the United States by Food and Drug Administration ( FDA). For young children or individuals with specific needs, it is painless and simple to use .The fluoride in SDF stimulates remineralization while the silver ions act as an antimicrobial agent when applied to carious lesions and stop cariogenic biofilms from growing. SDF also prevents future degradation of the dentin's collagen. With a 38 percent use rate, SDF has been utilized in a number of nations. According to a review, SDF is a harmless, economical caries control agent with a wide utilisation in dentistry, and it may meet the WHO Millennium Development Goal (MDG) as well as the United States Institute of Medicine's criteria. The applications of SDF in dental treatment are discussed in this article.


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