scholarly journals Silencing of DND1 in potato and tomato impedes conidial germination, attachment and hyphal growth of Botrytis cinerea

2017 ◽  
Vol 17 (1) ◽  
Author(s):  
Kaile Sun ◽  
Ageeth van Tuinen ◽  
Jan A. L. van Kan ◽  
Anne-Marie A. Wolters ◽  
Evert Jacobsen ◽  
...  
Keyword(s):  
Botrytis Cinerea ◽  
Hyphal Growth ◽  
Conidial Germination

scholarly journals Saprophytic microorganisms with potential for biological control of Botrytis cinerea on Geraldton waxflower flowers

2001 ◽  
Vol 41 (5) ◽  
pp. 697 ◽  
Author(s):  
D. R. Beasley ◽  
D. C. Joyce ◽  
L. M. Coates ◽  
A. H. Wearing
Keyword(s):  
Botrytis Cinerea ◽  
Mycelial Growth ◽  
Germ Tube ◽  
Conidial Germination ◽  
Pseudomonas Sp ◽  
Trichoderma Spp ◽  
Fusarium Sp ◽  
Flower Abscission ◽  
Germ Tube Elongation

Saprophytic bacteria, yeasts and filamentous fungi were isolated from Geraldton waxflower flowers and screened to identify potential antagonism towards Botrytis cinerea. Isolates from other sources (e.g. avocado) were also tested. Isolates were initially screened in vitro for inhibition of B. cinerea conidial germination, germ tube elongation and mycelial growth. The most antagonistic bacteria, yeasts and fungi were selected for further testing on detached waxflower flowers. Conidia of the pathogen were mixed with conidia or cells of the selected antagonists, co-inoculated onto waxflower flowers, and the flowers were sealed in glass jars and incubated at 20˚C. The number of days required for the pathogen to cause flower abscission was determined. The most antagonistic bacterial isolate, Pseudomonas sp. 677, significantly reduced conidial germination and retarded germ tube elongation of B. cinerea. None of the yeast or fungal isolates tested was found to significantly reduce conidial germination or retard germ tube elongation, but several significantly inhibited growth of B. cinerea. Fusarium sp., Epicoccum sp. and Trichoderma spp. were the most antagonistic of these isolates. Of the isolates tested on waxflower, Pseudomonas sp. 677 was highly antagonistic towards B. cinerea and delayed waxflower abscission by about 3 days. Trichoderma harzianum also significantly delayed flower abscission. However, as with most of the fungal antagonists used, inoculation of waxflower flowers with this isolate resulted in unsightly mycelial growth.

Morphological and chemical alterations inBotrytis cinereaexposed to the dicarboximide fungicide vinclozolin

1997 ◽  
Vol 43 (6) ◽  
pp. 552-560 ◽  
Author(s):  
Silvia M. J. C. S. Cabral ◽  
João P. S. Cabral
Keyword(s):  
Growth Rate ◽  
Chemical Composition ◽  
Botrytis Cinerea ◽  
Germ Tube ◽  
Membrane Damage ◽  
Hyphal Growth ◽  
Lower Content ◽  
Cell Debris ◽  
Biochemical Alterations ◽  
Severe Reduction

Treatment of actively growing Botrytis cinerea hyphae with micromolar concentrations of the dicarboximide fungicide vinclozolin resulted in significant alterations in the growth rate, morphology, and chemical composition of the cells. The addition of vinclozolin resulted in an immediate and severe reduction in the hyphal growth rate and a retardation in the emergence of the second germ tube. Cells treated with vinclozolin had a lower content of pool metabolites than control cells, and this difference increased with time of exposure to the fungicide. In contrast, vinclozolin-treated cells had a higher chitin concentration than control cells. These biochemical alterations were followed by the disorganization and clearing of cells, and by the appearance of dense and dark masses outside the hyphae, presumably composed of cell debris. Hyphae exposed to vinclozolin were more curved and branched and had shorter cells than the controls. The results indicate that vinclozolin causes a slow but generalized leakage of pool metabolites; this release precedes cell lysis and is not the result of a rapid and gross damage to the cytoplasmic membrane.Key words: vinclozolin, Botrytis cinerea, pool metabolites, membrane damage.

scholarly journals A Laboratory Simulation for Vectoring of Trichosporon pullulans by Conidia of Botrytis cinerea

2002 ◽  
Vol 92 (12) ◽  
pp. 1293-1299 ◽  
Author(s):  
Darryl W. M. Cook
Keyword(s):  
Botrytis Cinerea ◽  
Hyphal Growth ◽  
Yeast Cells ◽  
Laboratory Simulation ◽  
Disease Development ◽  
Stem Tissue ◽  
Growth Constraints

A mechanism that could contribute to the suppression of Botrytis cinerea during pathogen sporulation was examined in this study. Yeasts capable of binding to B. cinerea were formulated with a cellulose carrier and applied to sporulating colonies of the pathogen. The particles from this yeast/cellulose product attached to B. cinerea conidia in the sporulating colony. Inoculum from treated colonies was harvested and applied to tomato stem tissue to test for subsequent pathogenicity. Disease development from inoculum obtained from cultures that had been treated with Trichosporon pullulans was significantly retarded (P = 0.0001) compared with cellulose-only controls. However, between 5 and 11% of conidia applied were attached to yeast cells. The removal of conidia not attached to yeast resulted in inoculum composed of >90% of conidia attached to yeast, and from this inoculum, disease development was significantly retarded (P < 0.05). When inoculum from treated B. cinerea colonies was applied to nutrient limiting agar and then incubated, the B. cinerea conidia germinated, and yeast cells infested the new hyphal growth. Constraints of the formulation of the yeast used in this study, and the implications of this vectoring approach for the suppression of B. cinerea during pathogen sporulation are discussed.

scholarly journals Resistance to Boscalid Fungicide in Alternaria alternata Isolates from Pistachio in California

Plant Disease ◽  
2007 ◽  
Vol 91 (10) ◽  
pp. 1345-1350 ◽  
Author(s):  
Herve F. Avenot ◽  
Themis J. Michailides
Keyword(s):  
Spore Germination ◽  
Alternaria Alternata ◽  
Hyphal Growth ◽  
Conidial Germination ◽  
Prior History ◽  
In Vitro Tests ◽  
Fitness Parameters ◽  
History Of ◽  
The Mean

Boscalid is a new carboxamide fungicide recently introduced in a mixture with pyraclostrobin in the product Pristine for the control of Alternaria late blight of pistachio. In all, 108 isolates of Alternaria alternata were collected from pistachio orchards with (59 isolates) and without (49 isolates) prior exposure to boscalid. The sensitivity to boscalid was determined in conidial germination assays. The majority of isolates from two orchards without a prior history of boscalid usage had effective fungicide concentration to inhibit 50% of spore germination (EC50) values ranging from 0.089 to 3.435 μg/ml, and the mean EC50 was 1.515 μg/ml. Out of 59 isolates collected from an orchard with a history of boscalid usage, 52 isolates had EC50 values ranging from 0.055 to 4.222 μg/ml, and the mean EC50 was 1.214 μg/ml. However, in vitro tests for conidial germination and mycelial growth also revealed that seven A. alternata isolates, originating from the orchard exposed to boscalid were highly resistant (EC50 > 100 μg/ml) to this fungicide. Furthermore, in vitro tests showed no significant differences between wild-type and boscalid-resistant mutants in some fitness parameters such as spore germination, hyphal growth, sporulation, or virulence on pistachio leaves. Experiments on the stability of the boscalid-resistant phenotype showed no reduction of the resistance after the mutants were grown on fungicide-free medium. Preventative applications of a commercial formulation of boscalid (Endura) at a concentration which is effective against naturally sensitive isolates failed to control disease caused by the boscalid-resistant isolates in laboratory tests. To our knowledge, this is first report of field isolates of fungi resistant to boscalid.

Tetrandrine, a potent antifungal agent: inhibits mycelial growth and virulence of Botrytis cinerea

2020 ◽  
Author(s):  
Pingliang Li ◽  
jian zou ◽  
Yanhan Dong ◽  
jintao Jiang ◽  
Wenxing Liang ◽  
...  
Keyword(s):  
Botrytis Cinerea ◽  
Antifungal Agent ◽  
Plant Pathogens ◽  
Efflux Pump ◽  
Hyphal Growth ◽  
Tomato Plants ◽  
Low Concentrations ◽  
Grey Mold

Tetrandrine (TET) is a potent calcium channel blocker used for the treatment of hypertension and inflammation. Currently, TET is predominantly used to treat a variety of human diseases, and there is little information regarding the use of TET against plant pathogens. In this study, we explored the antifungal activity of TET on a plant pathogen, Botrytis cinerea. We show that administration of low concentrations of TET effectively inhibited hyphal growth of fungus grown on potato dextrose agarose, and decreased the virulence of B. cinerea in tomato plants. Real-time PCR revealed that the expression of drug efflux pump related genes (alcohol dehydrogenase 1, multi-drug/pheromone exporter, pleiotropic drug resistance protein 1, and synaptic vesicle transporter) were down-regulated in the presence of TET. Finally, we show that TET acts synergistically with iprodione, resulting in increased inhibition of B. cinerea both in vitro and in vivo. These results indicate that TET might act as an effective antifungal agent in reducing grey mold disease.

The Effects of Salinity on the Mosquito Pathogenic Fungus Culicinomyces.

1978 ◽  
Vol 26 (1) ◽  
pp. 55 ◽  
Author(s):  
AW Sweeney
Keyword(s):  
Nutrient Medium ◽  
Culture Medium ◽  
Sea Water ◽  
Pathogenic Fungus ◽  
Hyphal Growth ◽  
Conidial Germination ◽  
Australian Isolate ◽  
Mosquito Larvae

An Australian isolate of the fungus Culicinomyces grows best on nutrient medium without additional salt. Conidial germination and hyphal growth are progressively reduced as the salinity of the culture medium is increased, with very restricted growth at 1.5 times the salinity of sea water. In the laboratory, the fungus will kill mosquito larvae reared at a salinity half that of sea water, but not those reared in sea water, and it appears that the ability of conidia to invade the infection sites of the gut is impaired under the latter conditions.

Factors affecting postharvest infection of stored cabbage tissue by Botrytis cinerea

1975 ◽  
Vol 53 (8) ◽  
pp. 691-699 ◽  
Author(s):  
O. C. Yoder ◽  
M. L. Whalen
Keyword(s):  
Botrytis Cinerea ◽  
Nutrient Solution ◽  
Fungal Growth ◽  
Hyphal Growth ◽  
Optimum Temperature ◽  
Single Spore ◽  
Bacterial Populations ◽  
Factors Affecting ◽  
Successful Infection ◽  
Exogenous Source

Single-spore isolates of Botrytis cinerea from commercially stored cabbage exhibited a broad range of virulence. Spore germination and hyphal growth required an exogenous source of nutrients. The ability of either spores or mycelium to cause infection of stored cabbage tissue was also dependent upon exogenous nutrients. Nutrients required for infection could be supplied in the form of glucose, cabbage leaf extract, potato dextrose agar (PDA), or by a wound at the site of inoculation. The concentration of nutrients and (or) spores influenced the ability of B. cinerea to cause tissue decay whereas spore age and bacterial populations did not. Spores on the surface of cabbage leaves germinated within 4 to 8 h in nutrient solution; an additional 8 to 16 h in nutrient solution were required for successful infection. The optimum temperature for fungal growth and for infection was 20 to 25C. The optimum relative humidity for decay was above 97%; decay did not occur at relative humidities below 93%.

scholarly journals Antagonism of Trichoderma harzianum ETS 323 on Botrytis cinerea Mycelium in Culture Conditions

2012 ◽  
Vol 102 (11) ◽  
pp. 1054-1063 ◽  
Author(s):  
Chi-Hua Cheng ◽  
Chia-Ann Yang ◽  
Kou-Cheng Peng
Keyword(s):  
Amino Acid ◽  
Botrytis Cinerea ◽  
Trichoderma Harzianum ◽  
Hyphal Growth ◽  
Extracellular Proteins ◽  
In Vitro Assays ◽  
Acid Oxidase ◽  
Amino Acid Oxidase ◽  
Trichoderma Spp

Previous studies have shown that the extracellular proteins of Trichoderma harzianum ETS 323 grown in the presence of deactivated Botrytis cinerea in culture include a putative l-amino acid oxidase and have suggested the involvement of this enzyme in the antagonistic mechanism. Here, we hypothesized that the mycoparasitic process of Trichoderma spp. against B. cinerea involves two steps; that is, an initial hyphal coiling stage and a subsequent hyphal coiling stage, with different coiling rates. The two-step antagonism of T. harzianum ETS 323 against B. cinerea during the mycoparasitic process in culture was evaluated using a biexponential equation. In addition, an l-amino acid oxidase (Th-l-AAO) was identified from T. harzianum ETS 323. The secretion of Th-l-AAO was increased when T. harzianum ETS 323 was grown with deactivated hyphae of B. cinerea. Moreover, in vitro assays indicated that Th-l-AAO effectively inhibited B. cinerea hyphal growth, caused cytosolic vacuolization in the hyphae, and led to hyphal lysis. Th-l-AAO also showed disease control against the development of B. cinerea on postharvest apple fruit and tobacco leaves. Furthermore, an apoptosis-like response, including the generation of reactive oxygen species, was observed in B. cinerea after treatment with Th-l-AAO, suggesting that Th-l-AAO triggers programmed cell death in B. cinerea. This may be associated with the two-step antagonism of T. harzianum ETS 323 against B. cinerea.

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