Use of resuscitation promoting factors to screen for tuberculosis infection in household-exposed children in The Gambia

AbstractThe evidence of an association between diabetes and latent tuberculosis infection (LTBI) remains limited and inconsistent. Thus, the study aims to delineate the role of diabetes in activation of latent tuberculosis infection. Murine model of latent tuberculosis and diabetes was developed, bacillary load and gene expression of resuscitation promoting factors (rpfA-E) along with histopathological changes in the lungs and spleen were studied. Treatment for LTBI [Rifampicin (RIF) + Isoniazid (INH)] was also given to latently infected mice with or without diabetes for 4 weeks. Diabetes was found to activate latent tuberculosis as the colony forming unit (CFU) counts were observed to be > 104 in lungs and spleen. The gene expression of hspX was downregulated and that of rpfB and rpfD was observed to be upregulated in latently infected mice with diabetes compared to those without diabetes. However, no significant reduction in the CFU counts was observed after 4 weeks of treatment with RIF and INH. Diabetes helps in the progression of LTBI to active disease mainly through altered expression of resuscitation promoting factors rpfB and rpfD, which can serve as important targets to reduce the shared burden of tuberculosis and diabetes.

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Commercial Interferon Gamma Release Assays Compared to the Tuberculin Skin Test for Diagnosis of Latent Mycobacterium tuberculosis Infection in Childhood Contacts in the Gambia

The Pediatric Infectious Disease Journal ◽

10.1097/inf.0b013e3181cb45da ◽

2010 ◽

Vol 29 (5) ◽

pp. 439-443 ◽

Cited By ~ 31

Author(s):

Ifedayo M. O. Adetifa ◽

Martin O. C. Ota ◽

David J. Jeffries ◽

Abdulrahman Hammond ◽

Moses D. Lugos ◽

...

Keyword(s):

Mycobacterium Tuberculosis ◽

Tuberculin Skin Test ◽

Skin Test ◽

Interferon Gamma ◽

The Gambia ◽

Tuberculosis Infection ◽

Mycobacterium Tuberculosis Infection ◽

Interferon Gamma Release Assays

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Risk Factors for Tuberculosis Infection in Children in Contact With Infectious Tuberculosis Cases in The Gambia, West Africa

PEDIATRICS ◽

10.1542/peds.111.5.e608 ◽

2003 ◽

Vol 111 (5) ◽

pp. e608-e614 ◽

Cited By ~ 54

Author(s):

C. Lienhardt ◽

J. Sillah ◽

K. Fielding ◽

S. Donkor ◽

K. Manneh ◽

...

Keyword(s):

Risk Factors ◽

West Africa ◽

The Gambia ◽

Tuberculosis Infection

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BCG Vaccine Protection Against Mycobacterium tuberculosis Infection by Level of Exposure in The Gambia

The Journal of Infectious Diseases ◽

10.1093/infdis/jiaa411 ◽

2020 ◽

Author(s):

Nicola Campbell ◽

Ayesha J Verrall ◽

Simon Donkor ◽

Jayne S Sutherland ◽

Philip C Hill

Keyword(s):

Mycobacterium Tuberculosis ◽

Relative Risk ◽

Confidence Interval ◽

Poisson Regression ◽

The Gambia ◽

Bcg Vaccine ◽

Tuberculosis Infection ◽

Mycobacterium Tuberculosis Infection ◽

Vaccine Protection ◽

Pathogen Exposure

Abstract In Indonesia, BCG vaccine protection against Mycobacterium tuberculosis infection decreased with increasing exposure to the pathogen. We aimed to validate these findings in Africa. Poisson regression was used to estimate BCG protection, stratified by pathogen exposure using an exposure score, against enzyme-linked immunospot assay conversion at 3 months in 220 Gambian case contacts. Although the interaction between BCG and exposure was not significant (P = .13), BCG protection was strongest in the lowest-exposure tertile (relative risk, 0.35 [95% confidence interval, .15–.82; P = .02] vs 0.50 [.30–.83; P = .008] and 0.71 (.45–1.13; P = .1] for the middle and highest-exposure tertiles, respectively. These results are consistent with those from Indonesia.

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