scholarly journals Gridlock from diagnosis to treatment of multidrug-resistant tuberculosis in Tanzania: low accessibility of molecular diagnostic services and lack of healthcare worker empowerment in 28 districts of 5 high burden TB regions with mixed methods evaluation

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Stellah G. Mpagama ◽  
Peter M. Mbelele ◽  
Anna M. Chongolo ◽  
Isaack A. Lekule ◽  
Johnson J. Lyimo ◽  
...  
Keyword(s):  
Mixed Methods ◽  
Healthcare Worker ◽  
Multidrug Resistant ◽  
Molecular Diagnostic ◽  
High Burden ◽  
Resistant Tuberculosis ◽  
Mixed Methods Evaluation ◽  
Multidrug Resistant Tuberculosis ◽  
Worker Empowerment

scholarly journals Molecular Characterization of Multidrug-Resistant Mycobacterium tuberculosis Isolates from China

2014 ◽  
Vol 58 (4) ◽  
pp. 1997-2005 ◽  
Author(s):  
Li-Li Zhao ◽  
Yan Chen ◽  
Hai-Can Liu ◽  
Qiang Xia ◽  
Xiao-Cui Wu ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Multidrug Resistant ◽  
Diagnostic Methods ◽  
Molecular Diagnostic ◽  
Beijing Family ◽  
Phenotypic Data ◽  
Resistant Tuberculosis ◽  
Multidrug Resistant Tuberculosis ◽  
Mdr Tb

ABSTRACTTo investigate the molecular characterization of multidrug-resistant tuberculosis (MDR-TB) isolates from China and the association of specific mutations conferring drug resistance with strains of different genotypes, we performed spoligotyping and sequenced nine loci (katG,inhA, theoxyR-ahpCintergenic region,rpoB,tlyA,eis,rrs,gyrA, andgyrB) for 128 MDR-TB isolates. Our results showed that 108 isolates (84.4%) were Beijing family strains, 64 (59.3%) of which were identified as modern Beijing strains. Compared with the phenotypic data, the sensitivity and specificity of DNA sequencing were 89.1% and 100.0%, respectively, for isoniazid (INH) resistance, 93.8% and 100.0% for rifampin (RIF) resistance, 60.0% and 99.4% for capreomycin (CAP) resistance, 84.6% and 99.4% for kanamycin (KAN) resistance, and 90.0% and 100.0% for ofloxacin (OFX) resistance. The most prevalent mutations among the MDR-TB isolates werekatG315,inhA15,rpoB531, -526, and -516,rrs1401,eis-10, andgyrA94, -90, and -91. Furthermore, there was no association between specific resistance-conferring mutations and the strain genotype. These findings will be helpful for the establishment of rapid molecular diagnostic methods to be implemented in China.

GeneXpert contribution in the diagnosis of multi-resistant tuberculosis in new cases at the National University Hospital Centre of Pneumo-Phtisiology of Cotonou (Benin)

2021 ◽  
Vol 1 (2) ◽  
pp. 038-042
Author(s):  
Hebert Dedehouanou ◽  
Yazid Taïrou Toure ◽  
Laurence Yehouenou
Keyword(s):  
Pulmonary Tuberculosis ◽  
Multidrug Resistant ◽  
University Hospital ◽  
Molecular Diagnostic ◽  
The Third ◽  
Resistant Tuberculosis ◽  
Multidrug Resistant Tuberculosis ◽  
National University ◽  
Age Range ◽  
Limited Sensitivity

The diagnosis of bacteriologically confirmed pulmonary tuberculosis in Benin and in other developing countries relies on the search for acid-fast bacilli through microscopy despite its limited sensitivity. The objective of this study is to evaluate the contribution of the Xpert test in the diagnosis of multidrug-resistant tuberculosis in all new suspects received at the Centre National Hospitalier Universitaire de Pneumo-Phtisiologie de Cotonou (CNHU-PPC) of Benin. The biological material consisted of sputum from new suspected TB patients received at CNHU-PPC during the third quarter of 2018. A total of two thousand three hundred and seventy-five (2375) suspected patients were included in the study, of which 52% were men and 48% were women. The most representative age range was 15-24 years, 28%. GeneXpert was able to diagnose pulmonary tuberculosis in 10.02% of patients, including 9.7% rifampicin-susceptible and 0.3% rifampicin-resistant patients who could not be detected by microscopy. This molecular diagnostic tool is of great value for the diagnosis of multidrug-resistant tuberculosis in new suspects.

scholarly journals Multidrug-Resistant Tuberculosis Drug Susceptibility and Molecular Diagnostic Testing

2013 ◽  
Vol 345 (2) ◽  
pp. 143-148 ◽  
Author(s):  
Ameeta S. Kalokhe ◽  
James C. Lee ◽  
Susan M. Ray ◽  
Albert M. Anderson ◽  
Minh Ly T. Nguyen ◽  
...  
Keyword(s):  
Diagnostic Testing ◽  
Drug Susceptibility ◽  
Multidrug Resistant ◽  
Molecular Diagnostic ◽  
Resistant Tuberculosis ◽  
Multidrug Resistant Tuberculosis ◽  
Molecular Diagnostic Testing

scholarly journals Contact Investigation of Multidrug-Resistant Tuberculosis Patients: A Mixed-Methods Study from Myanmar

2019 ◽  
Vol 5 (1) ◽  
pp. 3
Author(s):  
Aye Mon Phyo ◽  
Ajay M. V. Kumar ◽  
Kyaw Thu Soe ◽  
Khine Wut Yee Kyaw ◽  
Aung Si Thu ◽  
...  
Keyword(s):  
Health Care ◽  
Mixed Methods ◽  
Health Care Providers ◽  
Multidrug Resistant ◽  
Mixed Methods Study ◽  
Care Providers ◽  
Resistant Tuberculosis ◽  
Multidrug Resistant Tuberculosis ◽  
Contact Investigation ◽  
Mdr Tb

There is no published evidence on contact investigation among multidrug-resistant tuberculosis (MDR-TB) patients from Myanmar. We describe the cascade of contact investigation conducted in 27 townships of Myanmar from January 2018 to June 2019 and its implementation challenges. This was a mixed-methods study involving quantitative (cohort analysis of programme data) and qualitative components (thematic analysis of interviews of 8 contacts and 13 health care providers). There were 556 MDR-TB patients and 1908 contacts, of whom 1134 (59%) reached the health centres for screening (chest radiography and symptoms). Of the latter, 344 (30%) had presumptive TB and of them, 186 (54%) were investigated (sputum microscopy or Xpert MTB/RIF®). A total of 27 TB patients were diagnosed (six bacteriologically-confirmed including five with rifampicin resistance). The key reasons for not reaching township TB centres included lack of knowledge and lack of risk perception owing to wrong beliefs among contacts, financial constraints related to loss of wages and transportation charges, and inconvenient clinic hours. The reasons for not being investigated included inability to produce sputum, health care providers being unaware of or not agreeing to the investigation protocol, fixed clinic days and times, and charges for investigation. The National Tuberculosis Programme needs to note these findings and take necessary action.

scholarly journals A Novel Molecular Strategy for Surveillance of Multidrug Resistant Tuberculosis in High Burden Settings

PLoS ONE ◽  
2016 ◽  
Vol 11 (1) ◽  
pp. e0146106 ◽  
Author(s):  
Halima M. Said ◽  
Nicole Kushner ◽  
Shaheed V. Omar ◽  
Andries W. Dreyer ◽  
Hendrik Koornhof ◽  
...  
Keyword(s):  
Multidrug Resistant ◽  
High Burden ◽  
Resistant Tuberculosis ◽  
Multidrug Resistant Tuberculosis
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